Sulfolobus monocaudavirus SMV1
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W0UUX6|W0UUX6_9VIRU Conserved archaeal viral protein OS=Sulfolobus monocaudavirus SMV1 OX=1351702 PE=4 SV=1
MM1 pKa = 7.65 GIIEE5 pKa = 5.02 EE6 pKa = 4.29 IQNALAYY13 pKa = 8.01 YY14 pKa = 10.17 QKK16 pKa = 9.75 TAVDD20 pKa = 4.14 EE21 pKa = 4.31 NDD23 pKa = 3.1 TLYY26 pKa = 11.31 LHH28 pKa = 7.04 NIYY31 pKa = 10.38 SQLYY35 pKa = 8.26 YY36 pKa = 10.13 ILSVEE41 pKa = 4.05 NRR43 pKa = 11.84 PLNNTEE49 pKa = 4.15 FDD51 pKa = 4.1 SLMKK55 pKa = 10.8 ALGINDD61 pKa = 3.72 DD62 pKa = 3.87 TLLVQEE68 pKa = 4.49 YY69 pKa = 10.71 QNMTDD74 pKa = 3.46 EE75 pKa = 4.31 QKK77 pKa = 10.87 KK78 pKa = 9.17 QLLMQILQMIDD89 pKa = 3.25 NLMKK93 pKa = 10.7 NIHH96 pKa = 5.51 TMVNYY101 pKa = 10.42
Molecular weight: 11.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.115
IPC2_protein 4.406
IPC_protein 4.279
Toseland 4.101
ProMoST 4.406
Dawson 4.24
Bjellqvist 4.393
Wikipedia 4.139
Rodwell 4.113
Grimsley 4.024
Solomon 4.228
Lehninger 4.19
Nozaki 4.355
DTASelect 4.533
Thurlkill 4.139
EMBOSS 4.151
Sillero 4.393
Patrickios 0.947
IPC_peptide 4.228
IPC2_peptide 4.38
IPC2.peptide.svr19 4.283
Protein with the highest isoelectric point:
>tr|W0UUC6|W0UUC6_9VIRU Uncharacterized protein OS=Sulfolobus monocaudavirus SMV1 OX=1351702 PE=4 SV=1
MM1 pKa = 7.59 RR2 pKa = 11.84 SKK4 pKa = 9.79 MRR6 pKa = 11.84 QGGKK10 pKa = 7.07 EE11 pKa = 3.76 ARR13 pKa = 11.84 GKK15 pKa = 9.71 GMRR18 pKa = 11.84 WSSINNISCSVNKK31 pKa = 10.35 LFGQPPVQLLFTLQLMGG48 pKa = 4.35
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.176
IPC2_protein 10.028
IPC_protein 11.374
Toseland 11.652
ProMoST 12.032
Dawson 11.667
Bjellqvist 11.579
Wikipedia 12.062
Rodwell 11.725
Grimsley 11.696
Solomon 12.076
Lehninger 11.989
Nozaki 11.637
DTASelect 11.579
Thurlkill 11.637
EMBOSS 12.12
Sillero 11.637
Patrickios 11.506
IPC_peptide 12.091
IPC2_peptide 11.038
IPC2.peptide.svr19 9.234
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
51
0
51
14287
48
2028
280.1
31.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.774 ± 0.428
0.434 ± 0.121
4.011 ± 0.43
5.942 ± 0.912
4.613 ± 0.385
5.236 ± 0.586
1.043 ± 0.128
8.588 ± 0.397
6.278 ± 0.728
10.023 ± 0.515
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.862 ± 0.24
6.481 ± 0.499
3.976 ± 0.389
5.089 ± 0.765
2.898 ± 0.445
8.238 ± 0.754
6.628 ± 0.596
7.041 ± 0.42
0.798 ± 0.188
5.04 ± 0.359
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here