Synechococcus phage ACG-2014f

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Atlauavirus; Synechococcus virus AC2014fSyn7803C8

Average proteome isoelectric point is 5.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 286 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3HK52|A0A0E3HK52_9CAUD Uncharacterized protein OS=Synechococcus phage ACG-2014f OX=1493511 GN=Syn7803US3_62 PE=4 SV=1
MM1 pKa = 6.87QRR3 pKa = 11.84SGVNYY8 pKa = 9.72KK9 pKa = 10.42VSGLDD14 pKa = 3.63LINRR18 pKa = 11.84DD19 pKa = 3.58TSILNAGDD27 pKa = 3.37VFLVEE32 pKa = 4.8RR33 pKa = 11.84SGTAYY38 pKa = 10.01KK39 pKa = 9.37FTVSLDD45 pKa = 3.13AGGDD49 pKa = 3.38VDD51 pKa = 4.1TSSIQDD57 pKa = 3.54DD58 pKa = 4.0DD59 pKa = 5.77LFVCFIDD66 pKa = 5.03GSEE69 pKa = 3.99KK70 pKa = 10.53KK71 pKa = 10.52INGLEE76 pKa = 4.03FSALVSPNVWEE87 pKa = 4.82TKK89 pKa = 10.52DD90 pKa = 3.31SWTHH94 pKa = 6.37IITDD98 pKa = 4.49IEE100 pKa = 4.47YY101 pKa = 9.19EE102 pKa = 3.99WQSVYY107 pKa = 10.95GYY109 pKa = 10.7DD110 pKa = 3.13HH111 pKa = 7.45DD112 pKa = 4.25GVYY115 pKa = 10.68RR116 pKa = 11.84LDD118 pKa = 3.61GTLIAAAASSTEE130 pKa = 3.96LTTEE134 pKa = 3.94SEE136 pKa = 4.62FVITGNSSTVSAGGIDD152 pKa = 4.43VRR154 pKa = 11.84VAWLSNIGTRR164 pKa = 11.84TDD166 pKa = 3.39LSRR169 pKa = 11.84AFQDD173 pKa = 5.22LPDD176 pKa = 3.68LTLFDD181 pKa = 5.54GYY183 pKa = 10.72WDD185 pKa = 3.68MTNVTNMAQMFYY197 pKa = 11.06NVGPFTADD205 pKa = 2.82VSSWNTSNVTNMSDD219 pKa = 2.71MFYY222 pKa = 10.69RR223 pKa = 11.84SGWGDD228 pKa = 3.12YY229 pKa = 8.89TFNVTGLSSWDD240 pKa = 3.73TTNVTCMSFMFEE252 pKa = 4.89GIDD255 pKa = 3.48LTEE258 pKa = 6.38DD259 pKa = 2.78ITGWDD264 pKa = 3.57VSNVTNMAGMFEE276 pKa = 4.38FGIFNQNIGGWDD288 pKa = 3.51VSNVTNMSYY297 pKa = 9.57MFHH300 pKa = 7.19EE301 pKa = 5.01SNTFNQNIGGWDD313 pKa = 3.5VANVTNMDD321 pKa = 3.07AMFYY325 pKa = 9.8YY326 pKa = 10.86YY327 pKa = 10.65SGGQFAGQFNQNLSGWCVTNIGSKK351 pKa = 10.84PNDD354 pKa = 3.25FDD356 pKa = 4.45TNSGFAGQTALQPQWGTCPP375 pKa = 3.39

Molecular weight: 41.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3HGP1|A0A0E3HGP1_9CAUD Uncharacterized protein OS=Synechococcus phage ACG-2014f OX=1493511 GN=Syn7803C12_50 PE=4 SV=1
MM1 pKa = 7.33TKK3 pKa = 9.29ATQRR7 pKa = 11.84PSTQSRR13 pKa = 11.84YY14 pKa = 7.1TSQVAMDD21 pKa = 4.51RR22 pKa = 11.84KK23 pKa = 10.07AAALQRR29 pKa = 11.84AEE31 pKa = 4.2EE32 pKa = 4.0RR33 pKa = 11.84RR34 pKa = 11.84LTGSADD40 pKa = 3.02VDD42 pKa = 3.78QVNRR46 pKa = 11.84LYY48 pKa = 10.68TVTLL52 pKa = 3.77

Molecular weight: 5.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

286

0

286

68578

40

4225

239.8

26.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.846 ± 0.212

0.949 ± 0.085

6.648 ± 0.138

6.486 ± 0.28

4.445 ± 0.124

8.09 ± 0.345

1.627 ± 0.127

6.483 ± 0.185

5.607 ± 0.345

7.294 ± 0.139

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.145 ± 0.188

5.72 ± 0.169

3.963 ± 0.123

3.366 ± 0.093

4.198 ± 0.141

7.301 ± 0.187

7.357 ± 0.371

7.15 ± 0.234

1.111 ± 0.108

4.214 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski