Oak-Vale virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Sripuvirus; Almpiwar sripuvirus

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G1BWF1|G1BWF1_9RHAB Putative SH protein OS=Oak-Vale virus OX=318852 GN=SH PE=4 SV=1
MM1 pKa = 7.27NLSPEE6 pKa = 4.11QLTEE10 pKa = 3.82LSSFVTQHH18 pKa = 5.6QLEE21 pKa = 4.55DD22 pKa = 4.66DD23 pKa = 3.96EE24 pKa = 7.18DD25 pKa = 4.17SLAEE29 pKa = 4.48KK30 pKa = 10.0VAKK33 pKa = 9.98SCSISEE39 pKa = 4.33KK40 pKa = 10.36VFPPNPSDD48 pKa = 3.64TEE50 pKa = 4.29GEE52 pKa = 4.01IMDD55 pKa = 3.96YY56 pKa = 10.59QSSGEE61 pKa = 4.11SDD63 pKa = 3.41GQANPSLEE71 pKa = 3.92WDD73 pKa = 3.53HH74 pKa = 6.99SVLARR79 pKa = 11.84AGDD82 pKa = 3.9SDD84 pKa = 3.55HH85 pKa = 6.48TRR87 pKa = 11.84PGGSLPTPGPSGTGGPSGLIGGGSGLIAIPQTPPAIIHH125 pKa = 5.66QPPVDD130 pKa = 4.11ASPPLIPAIPSQVTLTLADD149 pKa = 3.5AMSLFNSVLAKK160 pKa = 10.2SDD162 pKa = 3.3EE163 pKa = 4.14GSFYY167 pKa = 11.27LEE169 pKa = 4.35AGQIKK174 pKa = 10.26YY175 pKa = 10.38LSKK178 pKa = 10.78KK179 pKa = 9.01KK180 pKa = 9.62QQNLTRR186 pKa = 11.84SVLSGPDD193 pKa = 3.16SPLPGMEE200 pKa = 4.76SSPKK204 pKa = 8.4PQAVSRR210 pKa = 11.84GCQTTSSCIATSKK223 pKa = 10.63PPTPPPRR230 pKa = 11.84RR231 pKa = 11.84TLAANFSTPLAPPEE245 pKa = 4.0KK246 pKa = 9.67SKK248 pKa = 11.48SSGEE252 pKa = 4.07RR253 pKa = 11.84EE254 pKa = 3.99AVIPPTGIAWNPFCIFPEE272 pKa = 4.28PPGNKK277 pKa = 9.17DD278 pKa = 3.07GKK280 pKa = 9.26EE281 pKa = 3.8ASPEE285 pKa = 3.92STHH288 pKa = 8.14IEE290 pKa = 3.9EE291 pKa = 5.14HH292 pKa = 6.62PPGDD296 pKa = 3.23TGGNPFVQNPEE307 pKa = 3.51GAQIVDD313 pKa = 4.22RR314 pKa = 11.84PTAPPLGRR322 pKa = 11.84DD323 pKa = 3.64GAISNYY329 pKa = 10.07AGSPPNVPRR338 pKa = 11.84YY339 pKa = 10.19SLTEE343 pKa = 3.75LRR345 pKa = 11.84VKK347 pKa = 10.31SEE349 pKa = 3.84KK350 pKa = 10.48GFKK353 pKa = 10.04IKK355 pKa = 10.5GLNKK359 pKa = 9.83ILTIYY364 pKa = 10.32DD365 pKa = 3.72FRR367 pKa = 11.84WDD369 pKa = 3.72EE370 pKa = 4.21MTSHH374 pKa = 6.22QGKK377 pKa = 9.24FPLKK381 pKa = 9.36FWLRR385 pKa = 11.84FANII389 pKa = 3.42

Molecular weight: 41.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G1BWE8|G1BWE8_9RHAB Putative phosphoprotein OS=Oak-Vale virus OX=318852 GN=P PE=4 SV=1
MM1 pKa = 7.43NLLKK5 pKa = 10.47FFSSGDD11 pKa = 3.61PLAVSSAARR20 pKa = 11.84RR21 pKa = 11.84NARR24 pKa = 11.84ISYY27 pKa = 10.35SLDD30 pKa = 2.75ITLDD34 pKa = 3.62LSRR37 pKa = 11.84INVSLDD43 pKa = 3.06HH44 pKa = 6.83KK45 pKa = 11.19NVIEE49 pKa = 4.54AIKK52 pKa = 10.42RR53 pKa = 11.84VISPPLEE60 pKa = 3.86IRR62 pKa = 11.84SLICYY67 pKa = 9.36LLTSIRR73 pKa = 11.84TFRR76 pKa = 11.84VIKK79 pKa = 10.79GEE81 pKa = 3.9TRR83 pKa = 11.84TVGIKK88 pKa = 9.48WEE90 pKa = 4.16LSKK93 pKa = 10.7LTVPTDD99 pKa = 3.64FPEE102 pKa = 4.98RR103 pKa = 11.84FDD105 pKa = 4.05KK106 pKa = 10.74RR107 pKa = 11.84WIVHH111 pKa = 6.25RR112 pKa = 11.84EE113 pKa = 4.0GILPGFDD120 pKa = 3.0RR121 pKa = 11.84PIMYY125 pKa = 9.62KK126 pKa = 9.14VHH128 pKa = 6.78ICIEE132 pKa = 4.35PGPGGTAPDD141 pKa = 4.16FKK143 pKa = 10.62ILSLMSEE150 pKa = 4.14EE151 pKa = 3.96EE152 pKa = 3.83RR153 pKa = 11.84RR154 pKa = 11.84SNFTQRR160 pKa = 11.84DD161 pKa = 3.78RR162 pKa = 11.84GWVFNN167 pKa = 4.48

Molecular weight: 19.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

1

7

3738

60

2051

534.0

60.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.735 ± 0.874

2.06 ± 0.259

5.056 ± 0.244

6.26 ± 0.203

4.334 ± 0.284

7.143 ± 0.445

2.327 ± 0.304

7.598 ± 0.523

6.18 ± 1.047

9.524 ± 0.823

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.622 ± 0.378

4.361 ± 0.562

5.297 ± 1.453

3.264 ± 0.544

5.752 ± 0.911

7.571 ± 0.945

5.805 ± 0.332

5.778 ± 0.455

1.418 ± 0.132

2.916 ± 0.427
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski