Okra enation leaf curl virus [India:Munthal EL37:2006]
Average proteome isoelectric point is 8.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E7BNF4|E7BNF4_9GEMI AC4 protein OS=Okra enation leaf curl virus [India:Munthal EL37:2006] OX=908127 GN=AC4 PE=3 SV=1
MM1 pKa = 7.32 PPKK4 pKa = 10.41 RR5 pKa = 11.84 FLINSKK11 pKa = 10.69 NYY13 pKa = 8.83 FLTYY17 pKa = 9.04 PKK19 pKa = 10.47 CSLTRR24 pKa = 11.84 EE25 pKa = 4.12 EE26 pKa = 5.1 ALSQIKK32 pKa = 10.16 NFQTPTSKK40 pKa = 10.57 KK41 pKa = 8.61 YY42 pKa = 10.77 IKK44 pKa = 9.74 ICRR47 pKa = 11.84 EE48 pKa = 3.53 LHH50 pKa = 6.26 EE51 pKa = 4.93 NGEE54 pKa = 4.28 PHH56 pKa = 6.63 LHH58 pKa = 5.78 VLIQIEE64 pKa = 4.64 GKK66 pKa = 9.93 YY67 pKa = 9.76 KK68 pKa = 10.4 CQNQRR73 pKa = 11.84 FFDD76 pKa = 3.97 LVSPSRR82 pKa = 11.84 SAHH85 pKa = 4.9 FHH87 pKa = 6.45 PNIQGAKK94 pKa = 9.25 SSSDD98 pKa = 3.11 VKK100 pKa = 11.24 SYY102 pKa = 10.79 IDD104 pKa = 3.7 KK105 pKa = 11.33 DD106 pKa = 3.49 GDD108 pKa = 3.67 TLEE111 pKa = 4.14 WGEE114 pKa = 3.84 FQIDD118 pKa = 3.21 GRR120 pKa = 11.84 SARR123 pKa = 11.84 GGQQTANDD131 pKa = 4.05 AYY133 pKa = 10.69 AAALNAGSKK142 pKa = 10.29 SEE144 pKa = 3.84 ALRR147 pKa = 11.84 VIKK150 pKa = 10.21 EE151 pKa = 4.18 LAPKK155 pKa = 10.3 DD156 pKa = 3.74 YY157 pKa = 11.2 VLQFHH162 pKa = 6.76 NLNANLDD169 pKa = 4.29 RR170 pKa = 11.84 IFTPPVEE177 pKa = 4.62 VYY179 pKa = 10.26 VSPFSSSSFDD189 pKa = 3.41 QVPEE193 pKa = 3.98 EE194 pKa = 4.13 LEE196 pKa = 3.58 EE197 pKa = 3.9 WAAEE201 pKa = 4.15 NVVDD205 pKa = 5.03 AAARR209 pKa = 11.84 PLRR212 pKa = 11.84 PQSIVIEE219 pKa = 4.44 GDD221 pKa = 3.12 SRR223 pKa = 11.84 TGKK226 pKa = 8.52 TMWARR231 pKa = 11.84 SLGPHH236 pKa = 6.71 NYY238 pKa = 10.18 LCGHH242 pKa = 7.37 LDD244 pKa = 4.41 LSPKK248 pKa = 9.97 IYY250 pKa = 10.86 SNEE253 pKa = 3.09 AWYY256 pKa = 10.82 NVIDD260 pKa = 4.88 DD261 pKa = 4.33 VDD263 pKa = 3.73 PHH265 pKa = 6.08 FLKK268 pKa = 10.67 HH269 pKa = 5.9 FKK271 pKa = 10.69 EE272 pKa = 4.43 FMGAQRR278 pKa = 11.84 DD279 pKa = 3.81 WQSNTKK285 pKa = 9.6 YY286 pKa = 10.57 GKK288 pKa = 9.12 PVQIKK293 pKa = 10.41 GGIPTIFLCNPGPNSSYY310 pKa = 11.37 KK311 pKa = 10.66 EE312 pKa = 3.86 FLDD315 pKa = 3.54 EE316 pKa = 5.43 DD317 pKa = 4.07 KK318 pKa = 11.59 NSALKK323 pKa = 10.39 NWAVKK328 pKa = 10.27 NAIFVTLEE336 pKa = 3.9 GPLYY340 pKa = 10.86 SGTNQSTAQGSEE352 pKa = 3.63 EE353 pKa = 4.06 SAQEE357 pKa = 4.0 EE358 pKa = 4.71 EE359 pKa = 4.57 SRR361 pKa = 11.84 SS362 pKa = 3.66
Molecular weight: 40.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.804
IPC2_protein 5.779
IPC_protein 5.83
Toseland 6.224
ProMoST 6.16
Dawson 6.084
Bjellqvist 6.097
Wikipedia 6.097
Rodwell 6.071
Grimsley 6.376
Solomon 6.097
Lehninger 6.084
Nozaki 6.351
DTASelect 6.547
Thurlkill 6.547
EMBOSS 6.517
Sillero 6.453
Patrickios 4.177
IPC_peptide 6.109
IPC2_peptide 6.415
IPC2.peptide.svr19 6.43
Protein with the highest isoelectric point:
>tr|E7BNF1|E7BNF1_9GEMI Replication enhancer OS=Okra enation leaf curl virus [India:Munthal EL37:2006] OX=908127 GN=AC3 PE=3 SV=1
MM1 pKa = 7.71 SKK3 pKa = 10.35 RR4 pKa = 11.84 PADD7 pKa = 3.52 IVISTPASKK16 pKa = 10.29 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 3.11 SPYY27 pKa = 8.62 STRR30 pKa = 11.84 AVAPTVRR37 pKa = 11.84 VTKK40 pKa = 10.7 SRR42 pKa = 11.84 MWANRR47 pKa = 11.84 PMYY50 pKa = 9.66 RR51 pKa = 11.84 KK52 pKa = 8.9 PRR54 pKa = 11.84 MYY56 pKa = 10.28 RR57 pKa = 11.84 IYY59 pKa = 10.41 RR60 pKa = 11.84 SPDD63 pKa = 3.0 VPRR66 pKa = 11.84 GCEE69 pKa = 4.34 GPCKK73 pKa = 10.35 VQSFDD78 pKa = 3.26 AKK80 pKa = 11.06 NDD82 pKa = 3.27 IGHH85 pKa = 6.11 MGKK88 pKa = 10.22 VICLSDD94 pKa = 3.33 VTRR97 pKa = 11.84 GIGLTHH103 pKa = 6.67 RR104 pKa = 11.84 VGKK107 pKa = 9.78 RR108 pKa = 11.84 FCVKK112 pKa = 10.55 SLICRR117 pKa = 11.84 EE118 pKa = 3.98 NIKK121 pKa = 9.48 TKK123 pKa = 10.41 NHH125 pKa = 5.77 TNSVMFWIVRR135 pKa = 11.84 DD136 pKa = 3.82 RR137 pKa = 11.84 RR138 pKa = 11.84 PTGTPYY144 pKa = 11.16 DD145 pKa = 3.75 FQQVFNVYY153 pKa = 10.67 DD154 pKa = 4.09 NEE156 pKa = 4.37 PSTATVKK163 pKa = 10.59 NDD165 pKa = 3.0 QRR167 pKa = 11.84 DD168 pKa = 3.36 RR169 pKa = 11.84 FQVLRR174 pKa = 11.84 RR175 pKa = 11.84 FQATVTGGQYY185 pKa = 10.56 ACKK188 pKa = 9.3 EE189 pKa = 3.82 QVPIRR194 pKa = 11.84 KK195 pKa = 9.13 FYY197 pKa = 10.68 RR198 pKa = 11.84 VNNYY202 pKa = 7.79 VVYY205 pKa = 10.32 NHH207 pKa = 6.0 QEE209 pKa = 3.43 AGKK212 pKa = 10.41 YY213 pKa = 8.39 EE214 pKa = 4.09 NHH216 pKa = 6.52 TEE218 pKa = 3.99 NALLLYY224 pKa = 7.29 MACTHH229 pKa = 7.07 ASNPVYY235 pKa = 9.99 ATLKK239 pKa = 8.67 VRR241 pKa = 11.84 SYY243 pKa = 10.98 FYY245 pKa = 11.25 DD246 pKa = 3.13 SVTNN250 pKa = 3.98
Molecular weight: 29.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.301
IPC2_protein 9.575
IPC_protein 9.94
Toseland 10.058
ProMoST 9.853
Dawson 10.306
Bjellqvist 10.043
Wikipedia 10.511
Rodwell 10.584
Grimsley 10.394
Solomon 10.335
Lehninger 10.292
Nozaki 10.116
DTASelect 10.028
Thurlkill 10.145
EMBOSS 10.482
Sillero 10.233
Patrickios 9.882
IPC_peptide 10.335
IPC2_peptide 9.063
IPC2.peptide.svr19 8.494
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1117
100
362
186.2
21.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.924 ± 0.928
2.507 ± 0.67
5.013 ± 0.242
4.834 ± 1.094
4.029 ± 0.489
5.013 ± 0.366
3.312 ± 0.65
5.103 ± 0.768
5.551 ± 0.836
7.341 ± 1.073
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.611 ± 0.427
5.819 ± 0.319
6.088 ± 0.61
5.192 ± 0.544
7.431 ± 1.418
8.953 ± 1.571
5.819 ± 1.3
5.998 ± 1.219
1.253 ± 0.188
4.208 ± 0.588
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here