Proteus phage vB_PvuS_Pm34

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1P8DTH0|A0A1P8DTH0_9CAUD Prophage repressor protein OS=Proteus phage vB_PvuS_Pm34 OX=1933093 GN=Pm34_25 PE=4 SV=1
MM1 pKa = 7.83AIVQFYY7 pKa = 9.97IAGGKK12 pKa = 10.35GEE14 pKa = 4.57DD15 pKa = 3.59PSGISEE21 pKa = 4.55DD22 pKa = 3.8NLYY25 pKa = 10.67EE26 pKa = 5.29LPDD29 pKa = 3.71DD30 pKa = 4.88HH31 pKa = 7.02NFSADD36 pKa = 4.22DD37 pKa = 4.8DD38 pKa = 4.56LDD40 pKa = 4.09SCIEE44 pKa = 3.89ACAEE48 pKa = 4.18YY49 pKa = 11.06YY50 pKa = 10.52HH51 pKa = 7.12ADD53 pKa = 3.35CDD55 pKa = 3.64GWEE58 pKa = 4.4DD59 pKa = 3.37RR60 pKa = 11.84WPLLFMLWIDD70 pKa = 3.79DD71 pKa = 3.82QYY73 pKa = 11.76LGTFEE78 pKa = 4.3VVRR81 pKa = 11.84EE82 pKa = 3.93FDD84 pKa = 3.79PVFSANKK91 pKa = 9.86VEE93 pKa = 4.06

Molecular weight:
10.68 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1P8DTD2|A0A1P8DTD2_9CAUD Uncharacterized protein OS=Proteus phage vB_PvuS_Pm34 OX=1933093 GN=Pm34_10 PE=4 SV=1
MM1 pKa = 7.86PPRR4 pKa = 11.84IPRR7 pKa = 11.84ACRR10 pKa = 11.84KK11 pKa = 9.34QGCAKK16 pKa = 7.67TTTEE20 pKa = 3.89RR21 pKa = 11.84NGYY24 pKa = 9.91CEE26 pKa = 4.41DD27 pKa = 3.77HH28 pKa = 7.16QNLGWKK34 pKa = 6.13THH36 pKa = 4.56QRR38 pKa = 11.84GKK40 pKa = 10.28SRR42 pKa = 11.84HH43 pKa = 3.89QRR45 pKa = 11.84GYY47 pKa = 8.14GTQWDD52 pKa = 4.04KK53 pKa = 11.49LRR55 pKa = 11.84ARR57 pKa = 11.84ILKK60 pKa = 9.49RR61 pKa = 11.84DD62 pKa = 3.24KK63 pKa = 10.88YY64 pKa = 10.98LCQEE68 pKa = 4.19CLRR71 pKa = 11.84AGRR74 pKa = 11.84ATEE77 pKa = 4.1AKK79 pKa = 9.42TVDD82 pKa = 3.64HH83 pKa = 7.27IIAKK87 pKa = 10.05AHH89 pKa = 6.35GGTDD93 pKa = 3.32AEE95 pKa = 4.66EE96 pKa = 4.26NLQSLCWPCHH106 pKa = 4.74RR107 pKa = 11.84AKK109 pKa = 9.75TAKK112 pKa = 9.84EE113 pKa = 3.88RR114 pKa = 11.84IKK116 pKa = 11.11

Molecular weight:
13.41 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

12127

21

1274

173.2

19.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.048 ± 0.487

1.27 ± 0.19

5.442 ± 0.311

7.314 ± 0.205

3.414 ± 0.245

6.539 ± 0.311

1.723 ± 0.15

6.399 ± 0.219

6.943 ± 0.341

7.702 ± 0.332

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.101 ± 0.206

5.484 ± 0.242

3.669 ± 0.244

4.585 ± 0.283

5.162 ± 0.261

6.96 ± 0.306

5.459 ± 0.267

6.102 ± 0.245

1.699 ± 0.148

2.985 ± 0.191

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski