Proteus phage vB_PvuS_Pm34
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1P8DTH0|A0A1P8DTH0_9CAUD Prophage repressor protein OS=Proteus phage vB_PvuS_Pm34 OX=1933093 GN=Pm34_25 PE=4 SV=1
MM1 pKa = 7.83 AIVQFYY7 pKa = 9.97 IAGGKK12 pKa = 10.35 GEE14 pKa = 4.57 DD15 pKa = 3.59 PSGISEE21 pKa = 4.55 DD22 pKa = 3.8 NLYY25 pKa = 10.67 EE26 pKa = 5.29 LPDD29 pKa = 3.71 DD30 pKa = 4.88 HH31 pKa = 7.02 NFSADD36 pKa = 4.22 DD37 pKa = 4.8 DD38 pKa = 4.56 LDD40 pKa = 4.09 SCIEE44 pKa = 3.89 ACAEE48 pKa = 4.18 YY49 pKa = 11.06 YY50 pKa = 10.52 HH51 pKa = 7.12 ADD53 pKa = 3.35 CDD55 pKa = 3.64 GWEE58 pKa = 4.4 DD59 pKa = 3.37 RR60 pKa = 11.84 WPLLFMLWIDD70 pKa = 3.79 DD71 pKa = 3.82 QYY73 pKa = 11.76 LGTFEE78 pKa = 4.3 VVRR81 pKa = 11.84 EE82 pKa = 3.93 FDD84 pKa = 3.79 PVFSANKK91 pKa = 9.86 VEE93 pKa = 4.06
Molecular weight: 10.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.806
IPC2_protein 3.63
IPC_protein 3.605
Toseland 3.389
ProMoST 3.757
Dawson 3.605
Bjellqvist 3.821
Wikipedia 3.567
Rodwell 3.439
Grimsley 3.312
Solomon 3.592
Lehninger 3.541
Nozaki 3.732
DTASelect 3.961
Thurlkill 3.465
EMBOSS 3.567
Sillero 3.732
Patrickios 0.362
IPC_peptide 3.579
IPC2_peptide 3.706
IPC2.peptide.svr19 3.727
Protein with the highest isoelectric point:
>tr|A0A1P8DTD2|A0A1P8DTD2_9CAUD Uncharacterized protein OS=Proteus phage vB_PvuS_Pm34 OX=1933093 GN=Pm34_10 PE=4 SV=1
MM1 pKa = 7.86 PPRR4 pKa = 11.84 IPRR7 pKa = 11.84 ACRR10 pKa = 11.84 KK11 pKa = 9.34 QGCAKK16 pKa = 7.67 TTTEE20 pKa = 3.89 RR21 pKa = 11.84 NGYY24 pKa = 9.91 CEE26 pKa = 4.41 DD27 pKa = 3.77 HH28 pKa = 7.16 QNLGWKK34 pKa = 6.13 THH36 pKa = 4.56 QRR38 pKa = 11.84 GKK40 pKa = 10.28 SRR42 pKa = 11.84 HH43 pKa = 3.89 QRR45 pKa = 11.84 GYY47 pKa = 8.14 GTQWDD52 pKa = 4.04 KK53 pKa = 11.49 LRR55 pKa = 11.84 ARR57 pKa = 11.84 ILKK60 pKa = 9.49 RR61 pKa = 11.84 DD62 pKa = 3.24 KK63 pKa = 10.88 YY64 pKa = 10.98 LCQEE68 pKa = 4.19 CLRR71 pKa = 11.84 AGRR74 pKa = 11.84 ATEE77 pKa = 4.1 AKK79 pKa = 9.42 TVDD82 pKa = 3.64 HH83 pKa = 7.27 IIAKK87 pKa = 10.05 AHH89 pKa = 6.35 GGTDD93 pKa = 3.32 AEE95 pKa = 4.66 EE96 pKa = 4.26 NLQSLCWPCHH106 pKa = 4.74 RR107 pKa = 11.84 AKK109 pKa = 9.75 TAKK112 pKa = 9.84 EE113 pKa = 3.88 RR114 pKa = 11.84 IKK116 pKa = 11.11
Molecular weight: 13.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.175
IPC2_protein 9.136
IPC_protein 9.136
Toseland 10.087
ProMoST 9.736
Dawson 10.218
Bjellqvist 9.897
Wikipedia 10.335
Rodwell 10.701
Grimsley 10.262
Solomon 10.262
Lehninger 10.248
Nozaki 10.189
DTASelect 9.853
Thurlkill 10.116
EMBOSS 10.467
Sillero 10.175
Patrickios 10.438
IPC_peptide 10.262
IPC2_peptide 8.946
IPC2.peptide.svr19 7.98
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
70
0
70
12127
21
1274
173.2
19.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.048 ± 0.487
1.27 ± 0.19
5.442 ± 0.311
7.314 ± 0.205
3.414 ± 0.245
6.539 ± 0.311
1.723 ± 0.15
6.399 ± 0.219
6.943 ± 0.341
7.702 ± 0.332
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.101 ± 0.206
5.484 ± 0.242
3.669 ± 0.244
4.585 ± 0.283
5.162 ± 0.261
6.96 ± 0.306
5.459 ± 0.267
6.102 ± 0.245
1.699 ± 0.148
2.985 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here