Ustilaginoidea virens (Rice false smut fungus) (Villosiclava virens)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae;

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8421 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A063BTZ9|A0A063BTZ9_USTVR Thyroid hormone receptor interactor 12 OS=Ustilaginoidea virens OX=1159556 GN=UV8b_4930 PE=4 SV=1
MM1 pKa = 7.52FGTGEE6 pKa = 4.12IILANFDD13 pKa = 3.72VNSQLEE19 pKa = 4.46EE20 pKa = 3.83EE21 pKa = 5.27DD22 pKa = 3.78GANPYY27 pKa = 10.73DD28 pKa = 3.88NQDD31 pKa = 2.94GHH33 pKa = 8.48DD34 pKa = 3.88EE35 pKa = 4.11MGSVSTCLLWANITPGEE52 pKa = 4.11GVEE55 pKa = 4.32ACGSLTRR62 pKa = 11.84LSGTGPVSPIKK73 pKa = 10.4VDD75 pKa = 3.51VMASASDD82 pKa = 3.93VVDD85 pKa = 5.18GYY87 pKa = 9.48QNRR90 pKa = 11.84QHH92 pKa = 7.11DD93 pKa = 3.84

Molecular weight:
9.85 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A063BWQ8|A0A063BWQ8_USTVR Fungal transcriptional regulatory protein OS=Ustilaginoidea virens OX=1159556 GN=UV8b_4025 PE=4 SV=1
MM1 pKa = 7.39QSLTRR6 pKa = 11.84LVRR9 pKa = 11.84PLLRR13 pKa = 11.84TAVSSPRR20 pKa = 11.84PFTTLAPLRR29 pKa = 11.84PSLTPLRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84NGALASFTPSAPDD51 pKa = 3.39TAAGAAEE58 pKa = 4.07TADD61 pKa = 3.81LVSRR65 pKa = 11.84SAVSSHH71 pKa = 6.9PALAGLQLRR80 pKa = 11.84FGPRR84 pKa = 11.84NTMNGHH90 pKa = 5.05TRR92 pKa = 11.84LVQKK96 pKa = 10.2RR97 pKa = 11.84RR98 pKa = 11.84HH99 pKa = 5.48GFLHH103 pKa = 6.07RR104 pKa = 11.84TRR106 pKa = 11.84SRR108 pKa = 11.84SGRR111 pKa = 11.84RR112 pKa = 11.84ILLRR116 pKa = 11.84RR117 pKa = 11.84RR118 pKa = 11.84LKK120 pKa = 10.64GRR122 pKa = 11.84RR123 pKa = 11.84QLAQQ127 pKa = 3.1

Molecular weight:
14.1 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8421

0

8421

3909507

43

5938

464.3

50.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.855 ± 0.033

1.327 ± 0.011

5.882 ± 0.022

5.781 ± 0.035

3.361 ± 0.017

7.264 ± 0.031

2.509 ± 0.011

4.052 ± 0.023

4.635 ± 0.034

8.806 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.143 ± 0.011

3.242 ± 0.015

6.365 ± 0.034

4.187 ± 0.023

6.882 ± 0.029

8.228 ± 0.034

5.482 ± 0.03

6.235 ± 0.022

1.353 ± 0.01

2.41 ± 0.018

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski