Ustilaginoidea virens (Rice false smut fungus) (Villosiclava virens)
Average proteome isoelectric point is 6.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8421 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A063BTZ9|A0A063BTZ9_USTVR Thyroid hormone receptor interactor 12 OS=Ustilaginoidea virens OX=1159556 GN=UV8b_4930 PE=4 SV=1
MM1 pKa = 7.52 FGTGEE6 pKa = 4.12 IILANFDD13 pKa = 3.72 VNSQLEE19 pKa = 4.46 EE20 pKa = 3.83 EE21 pKa = 5.27 DD22 pKa = 3.78 GANPYY27 pKa = 10.73 DD28 pKa = 3.88 NQDD31 pKa = 2.94 GHH33 pKa = 8.48 DD34 pKa = 3.88 EE35 pKa = 4.11 MGSVSTCLLWANITPGEE52 pKa = 4.11 GVEE55 pKa = 4.32 ACGSLTRR62 pKa = 11.84 LSGTGPVSPIKK73 pKa = 10.4 VDD75 pKa = 3.51 VMASASDD82 pKa = 3.93 VVDD85 pKa = 5.18 GYY87 pKa = 9.48 QNRR90 pKa = 11.84 QHH92 pKa = 7.11 DD93 pKa = 3.84
Molecular weight: 9.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.751
IPC2_protein 3.808
IPC_protein 3.745
Toseland 3.554
ProMoST 3.846
Dawson 3.745
Bjellqvist 3.961
Wikipedia 3.681
Rodwell 3.579
Grimsley 3.465
Solomon 3.719
Lehninger 3.681
Nozaki 3.872
DTASelect 4.075
Thurlkill 3.617
EMBOSS 3.694
Sillero 3.872
Patrickios 1.875
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.817
Protein with the highest isoelectric point:
>tr|A0A063BWQ8|A0A063BWQ8_USTVR Fungal transcriptional regulatory protein OS=Ustilaginoidea virens OX=1159556 GN=UV8b_4025 PE=4 SV=1
MM1 pKa = 7.39 QSLTRR6 pKa = 11.84 LVRR9 pKa = 11.84 PLLRR13 pKa = 11.84 TAVSSPRR20 pKa = 11.84 PFTTLAPLRR29 pKa = 11.84 PSLTPLRR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 NGALASFTPSAPDD51 pKa = 3.39 TAAGAAEE58 pKa = 4.07 TADD61 pKa = 3.81 LVSRR65 pKa = 11.84 SAVSSHH71 pKa = 6.9 PALAGLQLRR80 pKa = 11.84 FGPRR84 pKa = 11.84 NTMNGHH90 pKa = 5.05 TRR92 pKa = 11.84 LVQKK96 pKa = 10.2 RR97 pKa = 11.84 RR98 pKa = 11.84 HH99 pKa = 5.48 GFLHH103 pKa = 6.07 RR104 pKa = 11.84 TRR106 pKa = 11.84 SRR108 pKa = 11.84 SGRR111 pKa = 11.84 RR112 pKa = 11.84 ILLRR116 pKa = 11.84 RR117 pKa = 11.84 RR118 pKa = 11.84 LKK120 pKa = 10.64 GRR122 pKa = 11.84 RR123 pKa = 11.84 QLAQQ127 pKa = 3.1
Molecular weight: 14.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 10.921
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.237
Grimsley 12.735
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 11.959
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.145
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8421
0
8421
3909507
43
5938
464.3
50.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.855 ± 0.033
1.327 ± 0.011
5.882 ± 0.022
5.781 ± 0.035
3.361 ± 0.017
7.264 ± 0.031
2.509 ± 0.011
4.052 ± 0.023
4.635 ± 0.034
8.806 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.143 ± 0.011
3.242 ± 0.015
6.365 ± 0.034
4.187 ± 0.023
6.882 ± 0.029
8.228 ± 0.034
5.482 ± 0.03
6.235 ± 0.022
1.353 ± 0.01
2.41 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here