Devosia sp. Root413D1
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5472 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0Q7DLJ2|A0A0Q7DLJ2_9RHIZ Succinylglutamate desuccinylase OS=Devosia sp. Root413D1 OX=1736531 GN=ASC89_26410 PE=4 SV=1
MM1 pKa = 7.27 NRR3 pKa = 11.84 FSLSLLAGVAVLSMASAASAADD25 pKa = 4.06 LLVQQPSMPGFVDD38 pKa = 3.49 MGSSGAWDD46 pKa = 3.6 GAYY49 pKa = 9.48 IGGFVGYY56 pKa = 9.9 GWGSANDD63 pKa = 3.81 DD64 pKa = 3.47 SGLFILGNDD73 pKa = 3.39 MDD75 pKa = 4.69 LSGWTAGATLGANFTVAQGFVLGAAGDD102 pKa = 4.02 IAWAGIGGFEE112 pKa = 5.24 APSQDD117 pKa = 4.49 VDD119 pKa = 4.06 INWTGSLRR127 pKa = 11.84 GKK129 pKa = 10.49 AGFDD133 pKa = 3.01 AGAFMPYY140 pKa = 8.87 LTAGLAFAGATASNGPTDD158 pKa = 3.62 STQMHH163 pKa = 6.84 FGWTAGAGVEE173 pKa = 4.46 VAVADD178 pKa = 4.58 QISLDD183 pKa = 3.54 LQYY186 pKa = 10.74 RR187 pKa = 11.84 YY188 pKa = 10.3 SDD190 pKa = 3.88 YY191 pKa = 10.89 GTATYY196 pKa = 10.47 DD197 pKa = 3.62 LPTNPEE203 pKa = 4.06 LDD205 pKa = 3.38 ISTHH209 pKa = 5.52 AVTAGVNFKK218 pKa = 10.65 FF219 pKa = 4.41
Molecular weight: 22.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.693
IPC2_protein 3.668
IPC_protein 3.681
Toseland 3.439
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.694
Rodwell 3.503
Grimsley 3.35
Solomon 3.681
Lehninger 3.643
Nozaki 3.834
DTASelect 4.139
Thurlkill 3.528
EMBOSS 3.706
Sillero 3.808
Patrickios 0.896
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.732
Protein with the highest isoelectric point:
>tr|A0A0Q7DSR7|A0A0Q7DSR7_9RHIZ Uncharacterized protein OS=Devosia sp. Root413D1 OX=1736531 GN=ASC89_14175 PE=4 SV=1
MM1 pKa = 7.31 MPTPRR6 pKa = 11.84 LRR8 pKa = 11.84 PKK10 pKa = 10.49 QPSLPNANASAHH22 pKa = 5.08 PTCNNVSPSPQAPPQSSSLRR42 pKa = 11.84 RR43 pKa = 11.84 PPKK46 pKa = 10.13 KK47 pKa = 9.29 PRR49 pKa = 11.84 HH50 pKa = 5.29 RR51 pKa = 11.84 LKK53 pKa = 10.71 SPRR56 pKa = 11.84 RR57 pKa = 11.84 QPRR60 pKa = 11.84 SQRR63 pKa = 11.84 LLLKK67 pKa = 10.27 NRR69 pKa = 11.84 HH70 pKa = 5.41 LPRR73 pKa = 11.84 LRR75 pKa = 11.84 NRR77 pKa = 11.84 PLPLNSLLQQSRR89 pKa = 11.84 PRR91 pKa = 11.84 LLNNPPSRR99 pKa = 11.84 LLPNSPPLPRR109 pKa = 11.84 SSLPLPWSSLQLPLNSRR126 pKa = 11.84 RR127 pKa = 11.84 PAIRR131 pKa = 11.84 RR132 pKa = 3.55
Molecular weight: 15.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 12.998
ProMoST 13.495
Dawson 12.998
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.603
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.495
Sillero 12.998
Patrickios 12.31
IPC_peptide 13.51
IPC2_peptide 12.501
IPC2.peptide.svr19 9.202
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5472
0
5472
1690778
27
9846
309.0
33.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.577 ± 0.049
0.629 ± 0.01
5.644 ± 0.04
5.59 ± 0.032
3.859 ± 0.026
8.805 ± 0.045
1.925 ± 0.017
5.323 ± 0.025
3.171 ± 0.029
10.437 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.347 ± 0.019
2.73 ± 0.028
5.114 ± 0.031
3.074 ± 0.017
6.413 ± 0.039
5.364 ± 0.026
5.526 ± 0.035
7.7 ± 0.025
1.429 ± 0.016
2.341 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here