Mucilaginibacter xinganensis
Average proteome isoelectric point is 7.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4924 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A223NVG2|A0A223NVG2_9SPHI Glyco_trans_2-like domain-containing protein OS=Mucilaginibacter xinganensis OX=1234841 GN=MuYL_1957 PE=4 SV=1
MM1 pKa = 7.19 YY2 pKa = 7.77 WTLEE6 pKa = 4.21 LASHH10 pKa = 7.01 LEE12 pKa = 4.08 DD13 pKa = 4.93 APWPATKK20 pKa = 10.54 DD21 pKa = 3.44 EE22 pKa = 5.26 LIDD25 pKa = 3.48 YY26 pKa = 8.56 AIRR29 pKa = 11.84 SGAPVEE35 pKa = 4.53 VIEE38 pKa = 4.56 NLQALEE44 pKa = 4.69 DD45 pKa = 4.28 DD46 pKa = 4.31 GEE48 pKa = 4.31 PYY50 pKa = 11.05 EE51 pKa = 4.65 NIEE54 pKa = 4.81 EE55 pKa = 4.08 IWPDD59 pKa = 3.85 YY60 pKa = 7.47 PTKK63 pKa = 10.94 DD64 pKa = 3.05 DD65 pKa = 4.15 FFFNEE70 pKa = 4.51 DD71 pKa = 3.17 EE72 pKa = 4.46 YY73 pKa = 11.99
Molecular weight: 8.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.818
IPC2_protein 3.592
IPC_protein 3.528
Toseland 3.338
ProMoST 3.694
Dawson 3.516
Bjellqvist 3.681
Wikipedia 3.439
Rodwell 3.363
Grimsley 3.261
Solomon 3.478
Lehninger 3.439
Nozaki 3.643
DTASelect 3.795
Thurlkill 3.401
EMBOSS 3.452
Sillero 3.656
Patrickios 0.693
IPC_peptide 3.478
IPC2_peptide 3.617
IPC2.peptide.svr19 3.629
Protein with the highest isoelectric point:
>tr|A0A223P0V7|A0A223P0V7_9SPHI Deaminase OS=Mucilaginibacter xinganensis OX=1234841 GN=MuYL_3583 PE=4 SV=1
MM1 pKa = 7.55 LHH3 pKa = 7.34 RR4 pKa = 11.84 LPDD7 pKa = 3.39 HH8 pKa = 6.63 TFGQVRR14 pKa = 11.84 WFKK17 pKa = 10.5 IPVRR21 pKa = 11.84 EE22 pKa = 3.97 GSVVHH27 pKa = 5.85 SRR29 pKa = 11.84 WSIVYY34 pKa = 9.25 GLSMFAKK41 pKa = 9.76 IFRR44 pKa = 11.84 RR45 pKa = 11.84 LIMITAVYY53 pKa = 9.54 LAAAPAHH60 pKa = 6.33 IIGNGFVCSDD70 pKa = 3.04 INIPAEE76 pKa = 4.05 YY77 pKa = 9.64 FVPEE81 pKa = 4.13 TLRR84 pKa = 11.84 TGVPAPHH91 pKa = 6.74 AFVRR95 pKa = 11.84 LQGITGWWCHH105 pKa = 4.95 WVIGLAVHH113 pKa = 6.65 GPWSMVHH120 pKa = 6.75 RR121 pKa = 11.84 RR122 pKa = 11.84 ILQQ125 pKa = 3.41
Molecular weight: 14.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.336
IPC2_protein 9.736
IPC_protein 10.745
Toseland 10.496
ProMoST 10.394
Dawson 10.672
Bjellqvist 10.482
Wikipedia 10.95
Rodwell 10.687
Grimsley 10.76
Solomon 10.818
Lehninger 10.76
Nozaki 10.54
DTASelect 10.467
Thurlkill 10.555
EMBOSS 10.921
Sillero 10.613
Patrickios 10.452
IPC_peptide 10.804
IPC2_peptide 9.809
IPC2.peptide.svr19 8.313
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4924
0
4924
1573549
37
2417
319.6
35.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.769 ± 0.036
0.824 ± 0.012
5.371 ± 0.025
5.23 ± 0.042
4.966 ± 0.026
6.955 ± 0.04
1.892 ± 0.016
7.383 ± 0.031
7.119 ± 0.033
9.418 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.282 ± 0.019
5.711 ± 0.035
3.934 ± 0.023
3.608 ± 0.027
3.704 ± 0.022
6.203 ± 0.028
5.851 ± 0.035
6.452 ± 0.026
1.179 ± 0.016
4.148 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here