Staphylococcus phage SN13
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A223LG77|A0A223LG77_9CAUD Uncharacterized protein OS=Staphylococcus phage SN13 OX=2024212 PE=4 SV=1
MM1 pKa = 7.48 LKK3 pKa = 8.95 STAQQALFDD12 pKa = 4.53 YY13 pKa = 10.26 IYY15 pKa = 10.92 SALEE19 pKa = 4.19 GYY21 pKa = 10.36 GFDD24 pKa = 4.27 VIDD27 pKa = 4.18 FKK29 pKa = 11.47 EE30 pKa = 4.32 LNTQISYY37 pKa = 10.27 PFFVVRR43 pKa = 11.84 NVDD46 pKa = 3.35 INKK49 pKa = 8.74 TKK51 pKa = 11.19 YY52 pKa = 11.07 NMDD55 pKa = 3.45 NFGGEE60 pKa = 3.97 LTATIDD66 pKa = 3.13 FWTYY70 pKa = 9.65 ADD72 pKa = 3.57 DD73 pKa = 4.5 RR74 pKa = 11.84 GRR76 pKa = 11.84 HH77 pKa = 5.41 DD78 pKa = 4.51 SIVYY82 pKa = 10.41 SVDD85 pKa = 3.4 TEE87 pKa = 4.0 FSNIDD92 pKa = 3.56 SVEE95 pKa = 4.31 GYY97 pKa = 10.74 QLMIDD102 pKa = 4.12 DD103 pKa = 5.04 MDD105 pKa = 3.62 IKK107 pKa = 10.07 TLNEE111 pKa = 3.99 VEE113 pKa = 4.16 NSDD116 pKa = 3.69 RR117 pKa = 11.84 QLLHH121 pKa = 5.95 TVFIANYY128 pKa = 10.15 KK129 pKa = 10.32 LFF131 pKa = 4.2
Molecular weight: 15.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.126
IPC2_protein 4.164
IPC_protein 4.126
Toseland 3.91
ProMoST 4.291
Dawson 4.113
Bjellqvist 4.266
Wikipedia 4.05
Rodwell 3.948
Grimsley 3.821
Solomon 4.113
Lehninger 4.062
Nozaki 4.228
DTASelect 4.482
Thurlkill 3.961
EMBOSS 4.062
Sillero 4.24
Patrickios 3.185
IPC_peptide 4.101
IPC2_peptide 4.228
IPC2.peptide.svr19 4.158
Protein with the highest isoelectric point:
>tr|A0A223LHW6|A0A223LHW6_9CAUD Uncharacterized protein OS=Staphylococcus phage SN13 OX=2024212 PE=4 SV=1
MM1 pKa = 7.44 ILSNSVRR8 pKa = 11.84 QRR10 pKa = 11.84 YY11 pKa = 8.35 RR12 pKa = 11.84 ADD14 pKa = 3.41 TAGKK18 pKa = 10.29 SPTEE22 pKa = 3.84 LQKK25 pKa = 10.28 EE26 pKa = 3.95 LRR28 pKa = 11.84 MRR30 pKa = 11.84 GVKK33 pKa = 10.39 GFVVNVNHH41 pKa = 6.25 NRR43 pKa = 11.84 VTMLVDD49 pKa = 3.12 RR50 pKa = 11.84 RR51 pKa = 11.84 DD52 pKa = 3.27 VKK54 pKa = 10.88 RR55 pKa = 11.84 NKK57 pKa = 9.93 EE58 pKa = 4.02 CMRR61 pKa = 4.66
Molecular weight: 7.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.239
IPC2_protein 9.853
IPC_protein 11.067
Toseland 11.301
ProMoST 11.725
Dawson 11.316
Bjellqvist 11.199
Wikipedia 11.681
Rodwell 11.33
Grimsley 11.345
Solomon 11.696
Lehninger 11.608
Nozaki 11.286
DTASelect 11.199
Thurlkill 11.286
EMBOSS 11.754
Sillero 11.286
Patrickios 11.096
IPC_peptide 11.696
IPC2_peptide 10.599
IPC2.peptide.svr19 9.234
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
71
0
71
12654
49
1039
178.2
20.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.061 ± 0.401
0.395 ± 0.074
6.575 ± 0.339
7.563 ± 0.547
4.307 ± 0.23
5.002 ± 0.27
1.667 ± 0.168
7.468 ± 0.316
9.357 ± 0.405
7.934 ± 0.324
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.363 ± 0.127
6.678 ± 0.232
2.584 ± 0.212
3.864 ± 0.245
4.117 ± 0.234
6.085 ± 0.307
6.014 ± 0.299
6.559 ± 0.255
1.019 ± 0.121
4.386 ± 0.351
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here