Tabrizicola piscis
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4119 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S8UBC0|A0A3S8UBC0_9RHOB Uncharacterized protein OS=Tabrizicola piscis OX=2494374 GN=EI545_20005 PE=4 SV=1
MM1 pKa = 7.57 SKK3 pKa = 10.31 HH4 pKa = 6.37 FSATRR9 pKa = 11.84 NNIAVPMPVLEE20 pKa = 4.35 TWTLPAALDD29 pKa = 4.23 DD30 pKa = 5.22 PGLTMADD37 pKa = 4.57 FGPVAAPSVTVEE49 pKa = 4.04 ATFATSPGCGCVGCSIGTTTEE70 pKa = 4.0 TSSSDD75 pKa = 3.09 TSGYY79 pKa = 10.87 AEE81 pKa = 4.49 NGSLATTTSVGLSGNAGIDD100 pKa = 3.39 ALLSGIRR107 pKa = 11.84 WASTISYY114 pKa = 9.85 SDD116 pKa = 3.42 PDD118 pKa = 3.46 SRR120 pKa = 11.84 FDD122 pKa = 3.7 YY123 pKa = 10.88 QSGYY127 pKa = 10.8 YY128 pKa = 9.72 VDD130 pKa = 4.8 SNGNGSSAQSEE141 pKa = 4.72 GFSQISPAMLAALRR155 pKa = 11.84 FALDD159 pKa = 3.04 ADD161 pKa = 4.25 AGVSAARR168 pKa = 11.84 GFAIEE173 pKa = 4.45 GFTNIKK179 pKa = 9.32 IDD181 pKa = 3.73 YY182 pKa = 8.98 AGSGSGAGTIRR193 pKa = 11.84 VANSSDD199 pKa = 3.31 APTAYY204 pKa = 10.31 AFYY207 pKa = 9.71 PDD209 pKa = 3.03 GSMYY213 pKa = 10.98 GGDD216 pKa = 3.48 SFFGTFARR224 pKa = 11.84 SPTAAGNYY232 pKa = 8.39 SWHH235 pKa = 6.03 TMLHH239 pKa = 5.47 EE240 pKa = 4.76 MGHH243 pKa = 5.57 SLGLKK248 pKa = 9.4 HH249 pKa = 6.11 GHH251 pKa = 5.65 EE252 pKa = 4.59 FSFGGALPANIDD264 pKa = 3.72 SNEE267 pKa = 3.95 YY268 pKa = 10.77 SVMTYY273 pKa = 10.4 RR274 pKa = 11.84 SFVGGTTQYY283 pKa = 10.16 ATWEE287 pKa = 4.21 TWGAPQTYY295 pKa = 9.18 MMYY298 pKa = 10.51 DD299 pKa = 3.5 IAALQHH305 pKa = 6.13 MYY307 pKa = 10.77 GADD310 pKa = 3.34 FSTNSGNTTYY320 pKa = 9.5 TWNPTNGQTLINGAVAINPGANRR343 pKa = 11.84 IFATIWDD350 pKa = 3.91 GGGIDD355 pKa = 4.27 TYY357 pKa = 11.67 NLSNYY362 pKa = 7.38 STNLLIDD369 pKa = 4.04 LTPGGHH375 pKa = 5.86 SVFSSAQLADD385 pKa = 4.39 LNDD388 pKa = 4.14 TNWIGLGPQYY398 pKa = 11.49 ARR400 pKa = 11.84 GNIFNALQFNGDD412 pKa = 3.45 SRR414 pKa = 11.84 SLIEE418 pKa = 4.06 NANGGSGNDD427 pKa = 3.3 MLLGNTANNLLNGGAGNDD445 pKa = 3.91 TLVGGAGNDD454 pKa = 3.66 TYY456 pKa = 11.4 VVSSGGDD463 pKa = 3.12 QVFEE467 pKa = 4.47 TTTTTSTTDD476 pKa = 2.67 AGGIDD481 pKa = 4.03 TVQSSISFRR490 pKa = 11.84 LDD492 pKa = 2.85 TTDD495 pKa = 4.28 GIRR498 pKa = 11.84 FVEE501 pKa = 4.05 NLTLTGSGNLSGTGNSLANILTGNAGNNTLFGGLGNDD538 pKa = 4.04 TLNGGAGNDD547 pKa = 3.84 TLNGGEE553 pKa = 5.33 DD554 pKa = 4.25 GDD556 pKa = 4.46 TMVGGAGNDD565 pKa = 3.65 LYY567 pKa = 11.44 VVSSTGDD574 pKa = 3.36 LVFEE578 pKa = 4.58 TTTTTSTTDD587 pKa = 2.67 AGGIDD592 pKa = 3.75 TVEE595 pKa = 4.24 SSVSFNLNANAGVRR609 pKa = 11.84 FVEE612 pKa = 3.97 NLTLTGTSAVEE623 pKa = 3.85 GTGNSRR629 pKa = 11.84 ANILTGNSGDD639 pKa = 3.83 NILRR643 pKa = 11.84 GAAGNDD649 pKa = 3.27 TLYY652 pKa = 11.48 GLDD655 pKa = 5.2 GNDD658 pKa = 3.79 TLNGGADD665 pKa = 3.82 SDD667 pKa = 4.39 TMVGGAGNDD676 pKa = 3.56 YY677 pKa = 11.11 YY678 pKa = 11.39 LVNSTGDD685 pKa = 3.18 RR686 pKa = 11.84 VFEE689 pKa = 4.42 TTTTTSTINAGGIDD703 pKa = 3.99 TVQSSVTFSLNTNAGVRR720 pKa = 11.84 FVEE723 pKa = 3.88 NLTLSGASAVNGTGNSLANTITGNTGANQLNGGLGSDD760 pKa = 3.31 ILTGGRR766 pKa = 11.84 GADD769 pKa = 2.87 SFVFNTALGSGNVDD783 pKa = 4.33 RR784 pKa = 11.84 ITDD787 pKa = 4.22 FDD789 pKa = 3.98 VLADD793 pKa = 4.18 SIRR796 pKa = 11.84 LEE798 pKa = 3.79 NAIFIGLSGGTLAASAFAANMTGDD822 pKa = 3.37 ATDD825 pKa = 3.13 ARR827 pKa = 11.84 DD828 pKa = 3.7 RR829 pKa = 11.84 IIYY832 pKa = 10.23 EE833 pKa = 3.9 SDD835 pKa = 2.87 TGNLYY840 pKa = 10.53 FDD842 pKa = 4.0 SDD844 pKa = 4.28 GTGSAAKK851 pKa = 10.17 VHH853 pKa = 6.5 FATVDD858 pKa = 3.22 TRR860 pKa = 11.84 VALTNADD867 pKa = 3.41 FFVFF871 pKa = 4.35
Molecular weight: 89.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.692
IPC2_protein 3.834
IPC_protein 3.884
Toseland 3.643
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.834
Rodwell 3.706
Grimsley 3.554
Solomon 3.884
Lehninger 3.846
Nozaki 3.986
DTASelect 4.291
Thurlkill 3.706
EMBOSS 3.846
Sillero 4.012
Patrickios 1.507
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.875
Protein with the highest isoelectric point:
>tr|A0A3S8U5E6|A0A3S8U5E6_9RHOB Serine/threonine protein phosphatase OS=Tabrizicola piscis OX=2494374 GN=EI545_07865 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.35 NGRR28 pKa = 11.84 LVLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.21 GRR39 pKa = 11.84 HH40 pKa = 5.51 KK41 pKa = 10.9 LSAA44 pKa = 3.8
Molecular weight: 5.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4119
0
4119
1303656
28
2823
316.5
34.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.256 ± 0.049
0.839 ± 0.01
5.837 ± 0.033
5.34 ± 0.031
3.628 ± 0.023
8.937 ± 0.041
2.033 ± 0.019
4.865 ± 0.026
2.834 ± 0.028
10.533 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.707 ± 0.02
2.28 ± 0.02
5.456 ± 0.03
3.036 ± 0.019
6.871 ± 0.039
4.836 ± 0.024
5.603 ± 0.026
7.561 ± 0.031
1.483 ± 0.019
2.065 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here