Burkholderia phage FLC5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Kisquattuordecimvirus; unclassified Kisquattuordecimvirus

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G1GMM5|A0A7G1GMM5_9CAUD Tail protein (X) OS=Burkholderia phage FLC5 OX=2716322 GN=ORF37 PE=4 SV=1
MM1 pKa = 7.64RR2 pKa = 11.84VYY4 pKa = 10.43AQQGDD9 pKa = 4.2TVDD12 pKa = 3.81ALCFRR17 pKa = 11.84YY18 pKa = 10.02LGRR21 pKa = 11.84TRR23 pKa = 11.84SVVEE27 pKa = 3.79LTLEE31 pKa = 4.26LNAGLADD38 pKa = 4.11YY39 pKa = 11.17GPVLPLGLAVDD50 pKa = 4.93LPDD53 pKa = 4.8PPSDD57 pKa = 3.08QSTIQLVNLFDD68 pKa = 3.64

Molecular weight: 7.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G1GM70|A0A7G1GM70_9CAUD Tail fiber assembly protein OS=Burkholderia phage FLC5 OX=2716322 GN=ORF23 PE=3 SV=1
MM1 pKa = 7.46PTMNTQQSTAKK12 pKa = 9.72RR13 pKa = 11.84VPIPLSAEE21 pKa = 4.19EE22 pKa = 3.97IDD24 pKa = 3.76ALQRR28 pKa = 11.84LAKK31 pKa = 9.65QDD33 pKa = 3.63QRR35 pKa = 11.84SEE37 pKa = 3.89AQMARR42 pKa = 11.84IIYY45 pKa = 9.71LAGLEE50 pKa = 4.05QYY52 pKa = 11.33ARR54 pKa = 11.84VIKK57 pKa = 10.44KK58 pKa = 10.46RR59 pKa = 11.84SRR61 pKa = 3.16

Molecular weight: 7.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

9698

30

923

215.5

23.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.776 ± 0.754

0.722 ± 0.172

6.414 ± 0.303

5.62 ± 0.297

3.279 ± 0.224

7.569 ± 0.494

1.939 ± 0.231

4.815 ± 0.166

4.362 ± 0.344

9.053 ± 0.238

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.495 ± 0.182

3.186 ± 0.188

4.238 ± 0.35

3.465 ± 0.236

6.857 ± 0.378

5.362 ± 0.238

6.496 ± 0.356

6.548 ± 0.274

1.279 ± 0.145

2.526 ± 0.209
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski