Mycobacteriophage ElTiger69

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 5.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I6WIH4|I6WIH4_9CAUD DNA binding protein OS=Mycobacteriophage ElTiger69 OX=1195069 GN=50 PE=4 SV=1
MM1 pKa = 7.41SRR3 pKa = 11.84EE4 pKa = 3.95TMQMAKK10 pKa = 9.84IYY12 pKa = 10.49VADD15 pKa = 4.18LAAYY19 pKa = 10.41NEE21 pKa = 4.31GSLVGRR27 pKa = 11.84WIDD30 pKa = 3.45LDD32 pKa = 3.56KK33 pKa = 11.27FSAVEE38 pKa = 4.44DD39 pKa = 3.4IVGEE43 pKa = 4.18IKK45 pKa = 9.1THH47 pKa = 5.02VLLAGHH53 pKa = 6.25EE54 pKa = 4.18EE55 pKa = 4.45WVIHH59 pKa = 5.83DD60 pKa = 4.53LEE62 pKa = 4.79GFGDD66 pKa = 3.68IRR68 pKa = 11.84ISEE71 pKa = 4.27YY72 pKa = 11.04EE73 pKa = 4.1DD74 pKa = 4.49LGTLLDD80 pKa = 3.98LSEE83 pKa = 5.71AIEE86 pKa = 4.04DD87 pKa = 3.69HH88 pKa = 6.8GDD90 pKa = 3.59PFVAWISEE98 pKa = 4.33VIGDD102 pKa = 3.6IGYY105 pKa = 8.54FANVADD111 pKa = 5.05AVDD114 pKa = 3.74QFEE117 pKa = 4.32DD118 pKa = 4.74RR119 pKa = 11.84YY120 pKa = 9.75VGPMTLEE127 pKa = 4.42DD128 pKa = 3.62YY129 pKa = 10.46AYY131 pKa = 9.72EE132 pKa = 4.09YY133 pKa = 11.37AEE135 pKa = 4.28EE136 pKa = 4.09VLGLKK141 pKa = 8.88DD142 pKa = 3.13TALDD146 pKa = 3.52YY147 pKa = 11.35FNYY150 pKa = 10.44EE151 pKa = 3.78KK152 pKa = 10.56FANDD156 pKa = 3.38LSMSGDD162 pKa = 3.42MTEE165 pKa = 3.85VYY167 pKa = 10.89YY168 pKa = 11.34NGVDD172 pKa = 3.47YY173 pKa = 11.07VFRR176 pKa = 11.84AWW178 pKa = 3.34

Molecular weight: 20.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I6XCX6|I6XCX6_9CAUD Uncharacterized protein OS=Mycobacteriophage ElTiger69 OX=1195069 GN=4 PE=4 SV=1
MM1 pKa = 7.51NKK3 pKa = 7.95QQKK6 pKa = 10.01VYY8 pKa = 10.41IDD10 pKa = 3.3GKK12 pKa = 10.78VYY14 pKa = 10.28VCRR17 pKa = 11.84GNNIVRR23 pKa = 11.84IRR25 pKa = 3.43

Molecular weight: 3.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

16017

25

937

174.1

19.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.847 ± 0.283

0.88 ± 0.11

6.999 ± 0.22

7.28 ± 0.309

3.309 ± 0.193

8.135 ± 0.379

2.042 ± 0.175

5.301 ± 0.176

4.664 ± 0.27

8.291 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.36 ± 0.122

3.484 ± 0.174

5.12 ± 0.252

3.378 ± 0.197

6.118 ± 0.326

5.656 ± 0.176

5.881 ± 0.239

7.224 ± 0.211

2.004 ± 0.135

3.028 ± 0.224
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski