Amino acid dipepetide frequency for Synechococcus phage ACG-2014d

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.921AlaAla: 5.921 ± 0.521
0.507AlaCys: 0.507 ± 0.112
4.611AlaAsp: 4.611 ± 0.3
3.825AlaGlu: 3.825 ± 0.275
2.585AlaPhe: 2.585 ± 0.239
6.218AlaGly: 6.218 ± 0.505
0.769AlaHis: 0.769 ± 0.111
4.087AlaIle: 4.087 ± 0.238
3.912AlaLys: 3.912 ± 0.458
4.646AlaLeu: 4.646 ± 0.339
1.467AlaMet: 1.467 ± 0.256
3.895AlaAsn: 3.895 ± 0.453
2.585AlaPro: 2.585 ± 0.22
2.847AlaGln: 2.847 ± 0.201
2.725AlaArg: 2.725 ± 0.289
5.275AlaSer: 5.275 ± 0.383
5.257AlaThr: 5.257 ± 0.536
3.703AlaVal: 3.703 ± 0.26
0.961AlaTrp: 0.961 ± 0.154
2.236AlaTyr: 2.236 ± 0.204
0.0AlaXaa: 0.0 ± 0.0
Cys
0.594CysAla: 0.594 ± 0.115
0.105CysCys: 0.105 ± 0.049
0.838CysAsp: 0.838 ± 0.121
0.611CysGlu: 0.611 ± 0.109
0.437CysPhe: 0.437 ± 0.095
0.541CysGly: 0.541 ± 0.105
0.245CysHis: 0.245 ± 0.082
0.681CysIle: 0.681 ± 0.126
0.559CysLys: 0.559 ± 0.097
0.611CysLeu: 0.611 ± 0.116
0.175CysMet: 0.175 ± 0.062
0.472CysAsn: 0.472 ± 0.088
0.367CysPro: 0.367 ± 0.089
0.279CysGln: 0.279 ± 0.079
0.507CysArg: 0.507 ± 0.111
0.803CysSer: 0.803 ± 0.136
0.646CysThr: 0.646 ± 0.126
0.629CysVal: 0.629 ± 0.134
0.14CysTrp: 0.14 ± 0.048
0.489CysTyr: 0.489 ± 0.099
0.0CysXaa: 0.0 ± 0.0
Asp
5.222AspAla: 5.222 ± 0.267
0.611AspCys: 0.611 ± 0.112
4.96AspAsp: 4.96 ± 0.296
4.105AspGlu: 4.105 ± 0.426
2.864AspPhe: 2.864 ± 0.225
6.235AspGly: 6.235 ± 0.477
0.838AspHis: 0.838 ± 0.121
4.244AspIle: 4.244 ± 0.384
3.249AspLys: 3.249 ± 0.285
4.96AspLeu: 4.96 ± 0.336
1.607AspMet: 1.607 ± 0.189
3.458AspAsn: 3.458 ± 0.271
3.563AspPro: 3.563 ± 0.258
2.533AspGln: 2.533 ± 0.214
2.585AspArg: 2.585 ± 0.17
4.244AspSer: 4.244 ± 0.338
4.436AspThr: 4.436 ± 0.39
4.367AspVal: 4.367 ± 0.332
0.891AspTrp: 0.891 ± 0.131
3.004AspTyr: 3.004 ± 0.313
0.0AspXaa: 0.0 ± 0.0
Glu
3.633GluAla: 3.633 ± 0.234
0.629GluCys: 0.629 ± 0.128
4.0GluAsp: 4.0 ± 0.331
4.454GluGlu: 4.454 ± 0.52
3.074GluPhe: 3.074 ± 0.201
3.912GluGly: 3.912 ± 0.257
0.961GluHis: 0.961 ± 0.165
4.629GluIle: 4.629 ± 0.314
3.528GluLys: 3.528 ± 0.503
5.17GluLeu: 5.17 ± 0.412
1.607GluMet: 1.607 ± 0.306
3.196GluAsn: 3.196 ± 0.249
2.009GluPro: 2.009 ± 0.205
2.323GluGln: 2.323 ± 0.207
2.62GluArg: 2.62 ± 0.264
4.017GluSer: 4.017 ± 0.283
3.563GluThr: 3.563 ± 0.28
4.541GluVal: 4.541 ± 0.266
0.821GluTrp: 0.821 ± 0.161
2.725GluTyr: 2.725 ± 0.259
0.0GluXaa: 0.0 ± 0.0
Phe
2.725PheAla: 2.725 ± 0.275
0.541PheCys: 0.541 ± 0.105
3.476PheAsp: 3.476 ± 0.257
2.55PheGlu: 2.55 ± 0.22
1.642PhePhe: 1.642 ± 0.215
2.655PheGly: 2.655 ± 0.285
0.559PheHis: 0.559 ± 0.125
2.969PheIle: 2.969 ± 0.237
2.48PheLys: 2.48 ± 0.21
2.69PheLeu: 2.69 ± 0.294
0.961PheMet: 0.961 ± 0.158
3.074PheAsn: 3.074 ± 0.232
1.555PhePro: 1.555 ± 0.17
1.729PheGln: 1.729 ± 0.166
1.712PheArg: 1.712 ± 0.141
3.284PheSer: 3.284 ± 0.243
3.336PheThr: 3.336 ± 0.266
2.62PheVal: 2.62 ± 0.233
0.349PheTrp: 0.349 ± 0.099
1.904PheTyr: 1.904 ± 0.189
0.0PheXaa: 0.0 ± 0.0
Gly
5.781GlyAla: 5.781 ± 0.525
0.716GlyCys: 0.716 ± 0.116
5.432GlyAsp: 5.432 ± 0.525
4.349GlyGlu: 4.349 ± 0.241
3.336GlyPhe: 3.336 ± 0.253
8.052GlyGly: 8.052 ± 0.701
0.961GlyHis: 0.961 ± 0.127
4.524GlyIle: 4.524 ± 0.4
4.122GlyLys: 4.122 ± 0.468
4.559GlyLeu: 4.559 ± 0.344
1.275GlyMet: 1.275 ± 0.233
4.227GlyAsn: 4.227 ± 0.457
2.131GlyPro: 2.131 ± 0.221
2.62GlyGln: 2.62 ± 0.186
2.987GlyArg: 2.987 ± 0.234
6.41GlySer: 6.41 ± 0.888
7.056GlyThr: 7.056 ± 0.745
4.594GlyVal: 4.594 ± 0.291
1.17GlyTrp: 1.17 ± 0.153
3.808GlyTyr: 3.808 ± 0.384
0.0GlyXaa: 0.0 ± 0.0
His
0.821HisAla: 0.821 ± 0.13
0.192HisCys: 0.192 ± 0.069
0.838HisAsp: 0.838 ± 0.157
0.891HisGlu: 0.891 ± 0.155
0.856HisPhe: 0.856 ± 0.135
1.065HisGly: 1.065 ± 0.139
0.367HisHis: 0.367 ± 0.097
0.926HisIle: 0.926 ± 0.134
0.891HisLys: 0.891 ± 0.157
0.734HisLeu: 0.734 ± 0.129
0.279HisMet: 0.279 ± 0.071
0.681HisAsn: 0.681 ± 0.135
0.908HisPro: 0.908 ± 0.115
0.594HisGln: 0.594 ± 0.107
0.699HisArg: 0.699 ± 0.148
0.786HisSer: 0.786 ± 0.137
0.873HisThr: 0.873 ± 0.116
0.803HisVal: 0.803 ± 0.104
0.175HisTrp: 0.175 ± 0.058
0.838HisTyr: 0.838 ± 0.156
0.017HisXaa: 0.017 ± 0.017
Ile
4.332IleAla: 4.332 ± 0.239
0.681IleCys: 0.681 ± 0.135
4.821IleAsp: 4.821 ± 0.481
4.559IleGlu: 4.559 ± 0.331
2.358IlePhe: 2.358 ± 0.205
4.035IleGly: 4.035 ± 0.32
0.454IleHis: 0.454 ± 0.116
3.493IleIle: 3.493 ± 0.305
3.86IleLys: 3.86 ± 0.358
4.367IleLeu: 4.367 ± 0.29
0.978IleMet: 0.978 ± 0.175
3.755IleAsn: 3.755 ± 0.261
3.354IlePro: 3.354 ± 0.216
2.795IleGln: 2.795 ± 0.215
2.288IleArg: 2.288 ± 0.217
4.943IleSer: 4.943 ± 0.478
5.38IleThr: 5.38 ± 0.695
4.279IleVal: 4.279 ± 0.344
0.576IleTrp: 0.576 ± 0.084
2.201IleTyr: 2.201 ± 0.186
0.0IleXaa: 0.0 ± 0.0
Lys
3.546LysAla: 3.546 ± 0.4
0.576LysCys: 0.576 ± 0.115
3.738LysAsp: 3.738 ± 0.447
3.965LysGlu: 3.965 ± 0.474
2.655LysPhe: 2.655 ± 0.233
3.249LysGly: 3.249 ± 0.4
1.048LysHis: 1.048 ± 0.202
3.476LysIle: 3.476 ± 0.263
4.96LysLys: 4.96 ± 0.664
4.367LysLeu: 4.367 ± 0.38
1.589LysMet: 1.589 ± 0.206
2.934LysAsn: 2.934 ± 0.384
2.166LysPro: 2.166 ± 0.293
2.026LysGln: 2.026 ± 0.308
2.096LysArg: 2.096 ± 0.255
3.843LysSer: 3.843 ± 0.389
3.685LysThr: 3.685 ± 0.243
4.367LysVal: 4.367 ± 0.361
0.716LysTrp: 0.716 ± 0.128
2.795LysTyr: 2.795 ± 0.312
0.0LysXaa: 0.0 ± 0.0
Leu
4.489LeuAla: 4.489 ± 0.289
0.751LeuCys: 0.751 ± 0.143
5.449LeuAsp: 5.449 ± 0.318
4.629LeuGlu: 4.629 ± 0.386
2.934LeuPhe: 2.934 ± 0.265
4.733LeuGly: 4.733 ± 0.325
1.275LeuHis: 1.275 ± 0.21
4.419LeuIle: 4.419 ± 0.279
4.332LeuLys: 4.332 ± 0.44
5.205LeuLeu: 5.205 ± 0.411
1.467LeuMet: 1.467 ± 0.235
4.768LeuAsn: 4.768 ± 0.267
2.795LeuPro: 2.795 ± 0.291
2.969LeuGln: 2.969 ± 0.22
2.882LeuArg: 2.882 ± 0.21
5.537LeuSer: 5.537 ± 0.319
5.292LeuThr: 5.292 ± 0.578
3.912LeuVal: 3.912 ± 0.229
0.699LeuTrp: 0.699 ± 0.129
3.126LeuTyr: 3.126 ± 0.257
0.0LeuXaa: 0.0 ± 0.0
Met
1.485MetAla: 1.485 ± 0.261
0.21MetCys: 0.21 ± 0.071
1.17MetAsp: 1.17 ± 0.16
1.45MetGlu: 1.45 ± 0.243
0.751MetPhe: 0.751 ± 0.114
1.397MetGly: 1.397 ± 0.248
0.332MetHis: 0.332 ± 0.082
1.065MetIle: 1.065 ± 0.156
1.52MetLys: 1.52 ± 0.283
1.694MetLeu: 1.694 ± 0.226
0.576MetMet: 0.576 ± 0.124
1.31MetAsn: 1.31 ± 0.234
0.803MetPro: 0.803 ± 0.15
0.996MetGln: 0.996 ± 0.199
0.978MetArg: 0.978 ± 0.158
1.485MetSer: 1.485 ± 0.248
1.362MetThr: 1.362 ± 0.183
1.135MetVal: 1.135 ± 0.185
0.314MetTrp: 0.314 ± 0.084
0.734MetTyr: 0.734 ± 0.139
0.0MetXaa: 0.0 ± 0.0
Asn
3.947AsnAla: 3.947 ± 0.373
0.681AsnCys: 0.681 ± 0.13
2.917AsnAsp: 2.917 ± 0.189
3.144AsnGlu: 3.144 ± 0.231
2.568AsnPhe: 2.568 ± 0.221
4.524AsnGly: 4.524 ± 0.438
0.629AsnHis: 0.629 ± 0.095
4.332AsnIle: 4.332 ± 0.361
3.319AsnLys: 3.319 ± 0.327
5.083AsnLeu: 5.083 ± 0.407
0.926AsnMet: 0.926 ± 0.185
3.616AsnAsn: 3.616 ± 0.33
3.196AsnPro: 3.196 ± 0.223
1.904AsnGln: 1.904 ± 0.194
2.271AsnArg: 2.271 ± 0.16
3.738AsnSer: 3.738 ± 0.254
4.646AsnThr: 4.646 ± 0.468
3.982AsnVal: 3.982 ± 0.313
0.664AsnTrp: 0.664 ± 0.113
2.69AsnTyr: 2.69 ± 0.231
0.017AsnXaa: 0.017 ± 0.018
Pro
2.568ProAla: 2.568 ± 0.217
0.454ProCys: 0.454 ± 0.129
2.864ProAsp: 2.864 ± 0.249
2.882ProGlu: 2.882 ± 0.295
1.851ProPhe: 1.851 ± 0.175
3.493ProGly: 3.493 ± 0.273
0.838ProHis: 0.838 ± 0.13
2.445ProIle: 2.445 ± 0.242
2.183ProLys: 2.183 ± 0.303
2.271ProLeu: 2.271 ± 0.225
0.699ProMet: 0.699 ± 0.157
2.148ProAsn: 2.148 ± 0.191
1.782ProPro: 1.782 ± 0.27
1.345ProGln: 1.345 ± 0.141
1.642ProArg: 1.642 ± 0.156
3.266ProSer: 3.266 ± 0.281
3.319ProThr: 3.319 ± 0.218
2.463ProVal: 2.463 ± 0.247
0.541ProTrp: 0.541 ± 0.125
1.782ProTyr: 1.782 ± 0.18
0.0ProXaa: 0.0 ± 0.0
Gln
2.271GlnAla: 2.271 ± 0.196
0.332GlnCys: 0.332 ± 0.082
2.306GlnAsp: 2.306 ± 0.189
2.375GlnGlu: 2.375 ± 0.248
1.712GlnPhe: 1.712 ± 0.204
2.795GlnGly: 2.795 ± 0.239
0.576GlnHis: 0.576 ± 0.085
2.41GlnIle: 2.41 ± 0.195
2.533GlnLys: 2.533 ± 0.311
3.039GlnLeu: 3.039 ± 0.279
1.223GlnMet: 1.223 ± 0.238
2.078GlnAsn: 2.078 ± 0.206
1.467GlnPro: 1.467 ± 0.149
1.345GlnGln: 1.345 ± 0.16
1.293GlnArg: 1.293 ± 0.189
2.428GlnSer: 2.428 ± 0.183
2.34GlnThr: 2.34 ± 0.246
2.533GlnVal: 2.533 ± 0.217
0.489GlnTrp: 0.489 ± 0.124
1.904GlnTyr: 1.904 ± 0.169
0.0GlnXaa: 0.0 ± 0.0
Arg
2.62ArgAla: 2.62 ± 0.285
0.419ArgCys: 0.419 ± 0.09
2.271ArgAsp: 2.271 ± 0.217
2.288ArgGlu: 2.288 ± 0.242
1.956ArgPhe: 1.956 ± 0.199
2.777ArgGly: 2.777 ± 0.251
0.699ArgHis: 0.699 ± 0.116
2.707ArgIle: 2.707 ± 0.202
2.358ArgLys: 2.358 ± 0.316
3.004ArgLeu: 3.004 ± 0.266
0.838ArgMet: 0.838 ± 0.157
1.851ArgAsn: 1.851 ± 0.206
1.397ArgPro: 1.397 ± 0.147
1.712ArgGln: 1.712 ± 0.19
1.589ArgArg: 1.589 ± 0.233
2.533ArgSer: 2.533 ± 0.196
2.41ArgThr: 2.41 ± 0.228
3.092ArgVal: 3.092 ± 0.241
0.454ArgTrp: 0.454 ± 0.116
1.799ArgTyr: 1.799 ± 0.205
0.0ArgXaa: 0.0 ± 0.0
Ser
5.415SerAla: 5.415 ± 0.317
0.559SerCys: 0.559 ± 0.098
4.489SerAsp: 4.489 ± 0.31
3.93SerGlu: 3.93 ± 0.274
3.388SerPhe: 3.388 ± 0.216
7.283SerGly: 7.283 ± 0.87
0.926SerHis: 0.926 ± 0.157
4.017SerIle: 4.017 ± 0.383
3.86SerLys: 3.86 ± 0.335
4.995SerLeu: 4.995 ± 0.227
1.555SerMet: 1.555 ± 0.251
4.908SerAsn: 4.908 ± 0.318
2.672SerPro: 2.672 ± 0.235
2.498SerGln: 2.498 ± 0.2
2.777SerArg: 2.777 ± 0.203
7.196SerSer: 7.196 ± 0.789
4.978SerThr: 4.978 ± 0.373
4.821SerVal: 4.821 ± 0.412
0.786SerTrp: 0.786 ± 0.122
3.161SerTyr: 3.161 ± 0.247
0.0SerXaa: 0.0 ± 0.0
Thr
5.153ThrAla: 5.153 ± 0.559
0.541ThrCys: 0.541 ± 0.104
4.332ThrAsp: 4.332 ± 0.409
4.244ThrGlu: 4.244 ± 0.245
3.161ThrPhe: 3.161 ± 0.332
6.602ThrGly: 6.602 ± 0.633
0.996ThrHis: 0.996 ± 0.121
5.362ThrIle: 5.362 ± 0.569
3.214ThrLys: 3.214 ± 0.263
6.131ThrLeu: 6.131 ± 0.526
1.031ThrMet: 1.031 ± 0.12
4.436ThrAsn: 4.436 ± 0.529
2.864ThrPro: 2.864 ± 0.236
2.306ThrGln: 2.306 ± 0.21
2.271ThrArg: 2.271 ± 0.19
5.292ThrSer: 5.292 ± 0.481
5.38ThrThr: 5.38 ± 0.744
6.008ThrVal: 6.008 ± 0.712
0.699ThrTrp: 0.699 ± 0.121
2.795ThrTyr: 2.795 ± 0.248
0.0ThrXaa: 0.0 ± 0.0
Val
4.297ValAla: 4.297 ± 0.302
0.454ValCys: 0.454 ± 0.072
5.03ValAsp: 5.03 ± 0.388
4.157ValGlu: 4.157 ± 0.254
2.568ValPhe: 2.568 ± 0.23
5.118ValGly: 5.118 ± 0.465
0.908ValHis: 0.908 ± 0.109
4.244ValIle: 4.244 ± 0.482
3.616ValLys: 3.616 ± 0.356
4.402ValLeu: 4.402 ± 0.256
1.205ValMet: 1.205 ± 0.185
4.192ValAsn: 4.192 ± 0.398
2.934ValPro: 2.934 ± 0.253
2.148ValGln: 2.148 ± 0.198
2.428ValArg: 2.428 ± 0.209
5.432ValSer: 5.432 ± 0.407
5.153ValThr: 5.153 ± 0.452
4.838ValVal: 4.838 ± 0.376
0.576ValTrp: 0.576 ± 0.089
2.725ValTyr: 2.725 ± 0.241
0.0ValXaa: 0.0 ± 0.0
Trp
0.699TrpAla: 0.699 ± 0.098
0.175TrpCys: 0.175 ± 0.056
0.891TrpAsp: 0.891 ± 0.118
0.541TrpGlu: 0.541 ± 0.109
0.384TrpPhe: 0.384 ± 0.099
0.734TrpGly: 0.734 ± 0.096
0.245TrpHis: 0.245 ± 0.073
0.716TrpIle: 0.716 ± 0.158
0.699TrpLys: 0.699 ± 0.123
0.734TrpLeu: 0.734 ± 0.108
0.454TrpMet: 0.454 ± 0.113
0.926TrpAsn: 0.926 ± 0.149
0.367TrpPro: 0.367 ± 0.083
0.664TrpGln: 0.664 ± 0.103
0.594TrpArg: 0.594 ± 0.127
0.891TrpSer: 0.891 ± 0.142
0.681TrpThr: 0.681 ± 0.096
0.734TrpVal: 0.734 ± 0.124
0.14TrpTrp: 0.14 ± 0.053
0.384TrpTyr: 0.384 ± 0.097
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.515TyrAla: 2.515 ± 0.237
0.594TyrCys: 0.594 ± 0.077
3.546TyrAsp: 3.546 ± 0.263
2.463TyrGlu: 2.463 ± 0.282
1.729TyrPhe: 1.729 ± 0.188
2.707TyrGly: 2.707 ± 0.225
0.611TyrHis: 0.611 ± 0.11
2.76TyrIle: 2.76 ± 0.2
2.602TyrLys: 2.602 ± 0.253
3.004TyrLeu: 3.004 ± 0.247
0.838TyrMet: 0.838 ± 0.142
2.864TyrAsn: 2.864 ± 0.264
1.904TyrPro: 1.904 ± 0.192
1.816TyrGln: 1.816 ± 0.174
1.834TyrArg: 1.834 ± 0.217
2.812TyrSer: 2.812 ± 0.237
3.022TyrThr: 3.022 ± 0.281
3.004TyrVal: 3.004 ± 0.241
0.489TyrTrp: 0.489 ± 0.137
1.851TyrTyr: 1.851 ± 0.178
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.017XaaPro: 0.017 ± 0.018
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.017XaaTyr: 0.017 ± 0.017
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 218 proteins (57254 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski