Amino acid dipepetide frequency for Achromatium sp. WMS2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.053AlaAla: 9.053 ± 0.152
1.013AlaCys: 1.013 ± 0.05
4.298AlaAsp: 4.298 ± 0.1
4.953AlaGlu: 4.953 ± 0.1
2.952AlaPhe: 2.952 ± 0.078
6.24AlaGly: 6.24 ± 0.133
1.776AlaHis: 1.776 ± 0.055
6.653AlaIle: 6.653 ± 0.114
4.51AlaLys: 4.51 ± 0.094
9.244AlaLeu: 9.244 ± 0.158
2.388AlaMet: 2.388 ± 0.07
4.334AlaAsn: 4.334 ± 0.085
3.398AlaPro: 3.398 ± 0.08
3.871AlaGln: 3.871 ± 0.105
4.423AlaArg: 4.423 ± 0.102
5.064AlaSer: 5.064 ± 0.103
5.501AlaThr: 5.501 ± 0.119
6.16AlaVal: 6.16 ± 0.128
1.248AlaTrp: 1.248 ± 0.044
2.314AlaTyr: 2.314 ± 0.065
0.0AlaXaa: 0.0 ± 0.0
Cys
0.816CysAla: 0.816 ± 0.04
0.178CysCys: 0.178 ± 0.019
0.528CysAsp: 0.528 ± 0.03
0.454CysGlu: 0.454 ± 0.031
0.367CysPhe: 0.367 ± 0.024
0.965CysGly: 0.965 ± 0.039
0.322CysHis: 0.322 ± 0.033
0.655CysIle: 0.655 ± 0.038
0.471CysLys: 0.471 ± 0.032
1.142CysLeu: 1.142 ± 0.043
0.226CysMet: 0.226 ± 0.019
0.499CysAsn: 0.499 ± 0.032
0.583CysPro: 0.583 ± 0.033
0.554CysGln: 0.554 ± 0.033
0.581CysArg: 0.581 ± 0.038
0.766CysSer: 0.766 ± 0.038
0.718CysThr: 0.718 ± 0.042
0.679CysVal: 0.679 ± 0.034
0.139CysTrp: 0.139 ± 0.016
0.398CysTyr: 0.398 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
4.313AspAla: 4.313 ± 0.094
0.537AspCys: 0.537 ± 0.031
2.196AspAsp: 2.196 ± 0.069
2.172AspGlu: 2.172 ± 0.061
2.059AspPhe: 2.059 ± 0.055
3.677AspGly: 3.677 ± 0.099
0.994AspHis: 0.994 ± 0.042
3.765AspIle: 3.765 ± 0.08
2.244AspLys: 2.244 ± 0.067
5.841AspLeu: 5.841 ± 0.106
1.143AspMet: 1.143 ± 0.044
2.402AspAsn: 2.402 ± 0.07
2.777AspPro: 2.777 ± 0.07
2.217AspGln: 2.217 ± 0.065
2.33AspArg: 2.33 ± 0.063
3.009AspSer: 3.009 ± 0.087
2.978AspThr: 2.978 ± 0.084
3.082AspVal: 3.082 ± 0.08
0.744AspTrp: 0.744 ± 0.036
1.834AspTyr: 1.834 ± 0.06
0.0AspXaa: 0.0 ± 0.0
Glu
4.447GluAla: 4.447 ± 0.111
0.477GluCys: 0.477 ± 0.03
2.326GluAsp: 2.326 ± 0.067
2.379GluGlu: 2.379 ± 0.07
1.85GluPhe: 1.85 ± 0.06
2.626GluGly: 2.626 ± 0.078
1.515GluHis: 1.515 ± 0.061
3.838GluIle: 3.838 ± 0.096
1.786GluLys: 1.786 ± 0.061
6.388GluLeu: 6.388 ± 0.119
1.054GluMet: 1.054 ± 0.043
1.85GluAsn: 1.85 ± 0.067
2.162GluPro: 2.162 ± 0.066
3.257GluGln: 3.257 ± 0.093
3.533GluArg: 3.533 ± 0.09
2.733GluSer: 2.733 ± 0.075
2.748GluThr: 2.748 ± 0.082
3.556GluVal: 3.556 ± 0.079
0.682GluTrp: 0.682 ± 0.034
1.639GluTyr: 1.639 ± 0.057
0.0GluXaa: 0.0 ± 0.0
Phe
3.242PheAla: 3.242 ± 0.076
0.417PheCys: 0.417 ± 0.025
2.105PheAsp: 2.105 ± 0.057
1.653PheGlu: 1.653 ± 0.056
1.49PhePhe: 1.49 ± 0.061
2.81PheGly: 2.81 ± 0.073
0.708PheHis: 0.708 ± 0.032
2.604PheIle: 2.604 ± 0.075
1.416PheLys: 1.416 ± 0.045
3.487PheLeu: 3.487 ± 0.094
0.883PheMet: 0.883 ± 0.043
1.611PheAsn: 1.611 ± 0.056
1.519PhePro: 1.519 ± 0.05
1.347PheGln: 1.347 ± 0.049
1.683PheArg: 1.683 ± 0.058
2.707PheSer: 2.707 ± 0.075
2.369PheThr: 2.369 ± 0.063
2.314PheVal: 2.314 ± 0.064
0.547PheTrp: 0.547 ± 0.03
1.258PheTyr: 1.258 ± 0.046
0.0PheXaa: 0.0 ± 0.0
Gly
5.467GlyAla: 5.467 ± 0.123
0.909GlyCys: 0.909 ± 0.043
3.172GlyAsp: 3.172 ± 0.079
3.067GlyGlu: 3.067 ± 0.068
2.892GlyPhe: 2.892 ± 0.071
4.891GlyGly: 4.891 ± 0.123
1.654GlyHis: 1.654 ± 0.056
5.561GlyIle: 5.561 ± 0.108
3.415GlyLys: 3.415 ± 0.075
7.486GlyLeu: 7.486 ± 0.13
1.831GlyMet: 1.831 ± 0.057
3.19GlyAsn: 3.19 ± 0.104
2.424GlyPro: 2.424 ± 0.068
2.844GlyGln: 2.844 ± 0.07
3.497GlyArg: 3.497 ± 0.074
4.337GlySer: 4.337 ± 0.106
4.32GlyThr: 4.32 ± 0.115
4.68GlyVal: 4.68 ± 0.096
0.965GlyTrp: 0.965 ± 0.046
2.638GlyTyr: 2.638 ± 0.082
0.0GlyXaa: 0.0 ± 0.0
His
1.776HisAla: 1.776 ± 0.059
0.298HisCys: 0.298 ± 0.026
1.099HisAsp: 1.099 ± 0.041
0.922HisGlu: 0.922 ± 0.038
0.847HisPhe: 0.847 ± 0.042
1.778HisGly: 1.778 ± 0.059
0.612HisHis: 0.612 ± 0.034
1.656HisIle: 1.656 ± 0.056
1.123HisLys: 1.123 ± 0.051
2.397HisLeu: 2.397 ± 0.061
0.487HisMet: 0.487 ± 0.031
1.224HisAsn: 1.224 ± 0.047
1.265HisPro: 1.265 ± 0.055
1.193HisGln: 1.193 ± 0.043
1.241HisArg: 1.241 ± 0.05
1.425HisSer: 1.425 ± 0.049
1.377HisThr: 1.377 ± 0.049
1.169HisVal: 1.169 ± 0.04
0.439HisTrp: 0.439 ± 0.029
0.802HisTyr: 0.802 ± 0.039
0.0HisXaa: 0.0 ± 0.0
Ile
6.864IleAla: 6.864 ± 0.122
0.852IleCys: 0.852 ± 0.038
3.76IleAsp: 3.76 ± 0.096
3.727IleGlu: 3.727 ± 0.086
2.721IlePhe: 2.721 ± 0.073
5.035IleGly: 5.035 ± 0.111
1.481IleHis: 1.481 ± 0.051
4.973IleIle: 4.973 ± 0.116
3.302IleLys: 3.302 ± 0.081
7.589IleLeu: 7.589 ± 0.126
1.443IleMet: 1.443 ± 0.048
3.454IleAsn: 3.454 ± 0.082
3.595IlePro: 3.595 ± 0.077
2.904IleGln: 2.904 ± 0.086
3.424IleArg: 3.424 ± 0.082
4.581IleSer: 4.581 ± 0.087
4.769IleThr: 4.769 ± 0.106
4.48IleVal: 4.48 ± 0.101
0.785IleTrp: 0.785 ± 0.04
1.893IleTyr: 1.893 ± 0.058
0.0IleXaa: 0.0 ± 0.0
Lys
3.677LysAla: 3.677 ± 0.089
0.357LysCys: 0.357 ± 0.026
2.319LysAsp: 2.319 ± 0.059
2.109LysGlu: 2.109 ± 0.068
1.673LysPhe: 1.673 ± 0.057
2.33LysGly: 2.33 ± 0.064
1.125LysHis: 1.125 ± 0.046
3.473LysIle: 3.473 ± 0.077
1.814LysLys: 1.814 ± 0.062
5.198LysLeu: 5.198 ± 0.101
0.963LysMet: 0.963 ± 0.037
2.071LysAsn: 2.071 ± 0.062
2.169LysPro: 2.169 ± 0.061
2.199LysGln: 2.199 ± 0.073
2.544LysArg: 2.544 ± 0.07
2.58LysSer: 2.58 ± 0.074
2.71LysThr: 2.71 ± 0.066
2.945LysVal: 2.945 ± 0.071
0.449LysTrp: 0.449 ± 0.026
1.505LysTyr: 1.505 ± 0.054
0.0LysXaa: 0.0 ± 0.0
Leu
10.896LeuAla: 10.896 ± 0.166
1.083LeuCys: 1.083 ± 0.044
6.103LeuAsp: 6.103 ± 0.097
6.286LeuGlu: 6.286 ± 0.133
3.684LeuPhe: 3.684 ± 0.102
7.754LeuGly: 7.754 ± 0.133
2.611LeuHis: 2.611 ± 0.069
7.217LeuIle: 7.217 ± 0.113
4.694LeuLys: 4.694 ± 0.086
11.275LeuLeu: 11.275 ± 0.214
2.223LeuMet: 2.223 ± 0.06
4.492LeuAsn: 4.492 ± 0.098
5.419LeuPro: 5.419 ± 0.106
4.853LeuGln: 4.853 ± 0.11
6.187LeuArg: 6.187 ± 0.125
6.343LeuSer: 6.343 ± 0.121
6.496LeuThr: 6.496 ± 0.101
6.991LeuVal: 6.991 ± 0.136
1.334LeuTrp: 1.334 ± 0.055
2.959LeuTyr: 2.959 ± 0.078
0.0LeuXaa: 0.0 ± 0.0
Met
2.253MetAla: 2.253 ± 0.063
0.183MetCys: 0.183 ± 0.019
1.245MetAsp: 1.245 ± 0.052
1.241MetGlu: 1.241 ± 0.047
0.766MetPhe: 0.766 ± 0.038
1.67MetGly: 1.67 ± 0.056
0.603MetHis: 0.603 ± 0.03
1.263MetIle: 1.263 ± 0.046
0.746MetLys: 0.746 ± 0.033
2.889MetLeu: 2.889 ± 0.069
0.427MetMet: 0.427 ± 0.023
0.888MetAsn: 0.888 ± 0.033
1.145MetPro: 1.145 ± 0.04
1.209MetGln: 1.209 ± 0.045
1.419MetArg: 1.419 ± 0.051
1.313MetSer: 1.313 ± 0.048
1.181MetThr: 1.181 ± 0.048
1.702MetVal: 1.702 ± 0.059
0.24MetTrp: 0.24 ± 0.02
0.545MetTyr: 0.545 ± 0.036
0.0MetXaa: 0.0 ± 0.0
Asn
3.573AsnAla: 3.573 ± 0.102
0.586AsnCys: 0.586 ± 0.038
1.821AsnAsp: 1.821 ± 0.054
1.697AsnGlu: 1.697 ± 0.062
1.812AsnPhe: 1.812 ± 0.061
2.808AsnGly: 2.808 ± 0.086
0.975AsnHis: 0.975 ± 0.045
3.528AsnIle: 3.528 ± 0.094
2.086AsnLys: 2.086 ± 0.058
5.273AsnLeu: 5.273 ± 0.111
1.212AsnMet: 1.212 ± 0.048
2.513AsnAsn: 2.513 ± 0.078
2.734AsnPro: 2.734 ± 0.079
2.169AsnGln: 2.169 ± 0.068
2.242AsnArg: 2.242 ± 0.072
3.11AsnSer: 3.11 ± 0.082
2.87AsnThr: 2.87 ± 0.079
2.527AsnVal: 2.527 ± 0.076
0.789AsnTrp: 0.789 ± 0.04
1.539AsnTyr: 1.539 ± 0.053
0.0AsnXaa: 0.0 ± 0.0
Pro
3.749ProAla: 3.749 ± 0.095
0.394ProCys: 0.394 ± 0.025
2.808ProAsp: 2.808 ± 0.07
3.355ProGlu: 3.355 ± 0.079
1.574ProPhe: 1.574 ± 0.052
3.288ProGly: 3.288 ± 0.084
1.008ProHis: 1.008 ± 0.036
3.161ProIle: 3.161 ± 0.071
2.405ProLys: 2.405 ± 0.068
4.33ProLeu: 4.33 ± 0.099
1.03ProMet: 1.03 ± 0.043
2.542ProAsn: 2.542 ± 0.073
2.319ProPro: 2.319 ± 0.09
2.319ProGln: 2.319 ± 0.068
1.889ProArg: 1.889 ± 0.056
2.532ProSer: 2.532 ± 0.065
2.865ProThr: 2.865 ± 0.085
3.362ProVal: 3.362 ± 0.077
0.667ProTrp: 0.667 ± 0.029
1.392ProTyr: 1.392 ± 0.051
0.0ProXaa: 0.0 ± 0.0
Gln
4.927GlnAla: 4.927 ± 0.127
0.427GlnCys: 0.427 ± 0.031
2.373GlnAsp: 2.373 ± 0.065
2.599GlnGlu: 2.599 ± 0.081
1.473GlnPhe: 1.473 ± 0.056
2.607GlnGly: 2.607 ± 0.078
1.442GlnHis: 1.442 ± 0.053
3.321GlnIle: 3.321 ± 0.081
1.593GlnLys: 1.593 ± 0.065
5.002GlnLeu: 5.002 ± 0.114
0.924GlnMet: 0.924 ± 0.039
1.783GlnAsn: 1.783 ± 0.056
2.254GlnPro: 2.254 ± 0.07
3.041GlnGln: 3.041 ± 0.109
2.971GlnArg: 2.971 ± 0.081
2.283GlnSer: 2.283 ± 0.066
2.484GlnThr: 2.484 ± 0.077
3.389GlnVal: 3.389 ± 0.075
0.53GlnTrp: 0.53 ± 0.038
1.311GlnTyr: 1.311 ± 0.049
0.0GlnXaa: 0.0 ± 0.0
Arg
4.06ArgAla: 4.06 ± 0.091
0.571ArgCys: 0.571 ± 0.031
2.662ArgAsp: 2.662 ± 0.066
2.871ArgGlu: 2.871 ± 0.086
1.973ArgPhe: 1.973 ± 0.055
3.202ArgGly: 3.202 ± 0.079
1.221ArgHis: 1.221 ± 0.05
4.046ArgIle: 4.046 ± 0.083
2.256ArgLys: 2.256 ± 0.074
6.237ArgLeu: 6.237 ± 0.125
1.323ArgMet: 1.323 ± 0.047
2.402ArgAsn: 2.402 ± 0.069
2.184ArgPro: 2.184 ± 0.068
2.487ArgGln: 2.487 ± 0.085
3.079ArgArg: 3.079 ± 0.082
3.017ArgSer: 3.017 ± 0.077
2.604ArgThr: 2.604 ± 0.076
3.288ArgVal: 3.288 ± 0.086
0.903ArgTrp: 0.903 ± 0.036
1.942ArgTyr: 1.942 ± 0.063
0.0ArgXaa: 0.0 ± 0.0
Ser
4.605SerAla: 4.605 ± 0.097
0.732SerCys: 0.732 ± 0.039
2.777SerAsp: 2.777 ± 0.078
2.77SerGlu: 2.77 ± 0.069
2.143SerPhe: 2.143 ± 0.061
5.128SerGly: 5.128 ± 0.124
1.342SerHis: 1.342 ± 0.049
4.464SerIle: 4.464 ± 0.099
3.108SerLys: 3.108 ± 0.075
6.22SerLeu: 6.22 ± 0.101
1.598SerMet: 1.598 ± 0.054
2.962SerAsn: 2.962 ± 0.081
2.582SerPro: 2.582 ± 0.059
2.702SerGln: 2.702 ± 0.069
2.746SerArg: 2.746 ± 0.077
4.029SerSer: 4.029 ± 0.107
3.595SerThr: 3.595 ± 0.09
3.617SerVal: 3.617 ± 0.085
0.955SerTrp: 0.955 ± 0.047
1.882SerTyr: 1.882 ± 0.052
0.0SerXaa: 0.0 ± 0.0
Thr
5.899ThrAla: 5.899 ± 0.118
0.699ThrCys: 0.699 ± 0.043
3.084ThrAsp: 3.084 ± 0.067
3.04ThrGlu: 3.04 ± 0.076
1.802ThrPhe: 1.802 ± 0.062
4.802ThrGly: 4.802 ± 0.119
1.157ThrHis: 1.157 ± 0.043
4.087ThrIle: 4.087 ± 0.086
2.794ThrLys: 2.794 ± 0.063
6.372ThrLeu: 6.372 ± 0.113
1.286ThrMet: 1.286 ± 0.046
2.878ThrAsn: 2.878 ± 0.09
3.319ThrPro: 3.319 ± 0.088
2.583ThrGln: 2.583 ± 0.085
2.508ThrArg: 2.508 ± 0.069
3.566ThrSer: 3.566 ± 0.096
3.739ThrThr: 3.739 ± 0.105
4.164ThrVal: 4.164 ± 0.096
0.847ThrTrp: 0.847 ± 0.044
1.569ThrTyr: 1.569 ± 0.067
0.0ThrXaa: 0.0 ± 0.0
Val
6.334ValAla: 6.334 ± 0.112
0.749ValCys: 0.749 ± 0.039
3.526ValAsp: 3.526 ± 0.088
3.64ValGlu: 3.64 ± 0.09
2.275ValPhe: 2.275 ± 0.063
4.576ValGly: 4.576 ± 0.104
1.421ValHis: 1.421 ± 0.05
4.678ValIle: 4.678 ± 0.095
2.681ValLys: 2.681 ± 0.076
7.061ValLeu: 7.061 ± 0.125
1.574ValMet: 1.574 ± 0.063
2.753ValAsn: 2.753 ± 0.069
2.904ValPro: 2.904 ± 0.081
2.445ValGln: 2.445 ± 0.068
3.338ValArg: 3.338 ± 0.081
3.694ValSer: 3.694 ± 0.086
4.545ValThr: 4.545 ± 0.11
4.773ValVal: 4.773 ± 0.108
0.768ValTrp: 0.768 ± 0.034
1.79ValTyr: 1.79 ± 0.052
0.0ValXaa: 0.0 ± 0.0
Trp
0.813TrpAla: 0.813 ± 0.039
0.206TrpCys: 0.206 ± 0.016
0.65TrpAsp: 0.65 ± 0.034
0.658TrpGlu: 0.658 ± 0.036
0.538TrpPhe: 0.538 ± 0.032
0.792TrpGly: 0.792 ± 0.039
0.406TrpHis: 0.406 ± 0.026
1.013TrpIle: 1.013 ± 0.048
0.492TrpLys: 0.492 ± 0.025
1.959TrpLeu: 1.959 ± 0.063
0.357TrpMet: 0.357 ± 0.026
0.559TrpAsn: 0.559 ± 0.034
0.588TrpPro: 0.588 ± 0.034
0.898TrpGln: 0.898 ± 0.039
0.825TrpArg: 0.825 ± 0.04
0.871TrpSer: 0.871 ± 0.04
0.603TrpThr: 0.603 ± 0.037
0.809TrpVal: 0.809 ± 0.037
0.219TrpTrp: 0.219 ± 0.022
0.468TrpTyr: 0.468 ± 0.028
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.63TyrAla: 2.63 ± 0.071
0.374TyrCys: 0.374 ± 0.027
1.462TyrAsp: 1.462 ± 0.056
1.253TyrGlu: 1.253 ± 0.043
1.281TyrPhe: 1.281 ± 0.049
2.263TyrGly: 2.263 ± 0.064
0.754TyrHis: 0.754 ± 0.038
1.673TyrIle: 1.673 ± 0.056
1.274TyrLys: 1.274 ± 0.049
3.511TyrLeu: 3.511 ± 0.09
0.554TyrMet: 0.554 ± 0.031
1.459TyrAsn: 1.459 ± 0.048
1.651TyrPro: 1.651 ± 0.056
1.641TyrGln: 1.641 ± 0.061
1.869TyrArg: 1.869 ± 0.055
1.997TyrSer: 1.997 ± 0.07
1.723TyrThr: 1.723 ± 0.061
1.838TyrVal: 1.838 ± 0.055
0.475TyrTrp: 0.475 ± 0.03
1.186TyrTyr: 1.186 ± 0.052
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2198 proteins (583320 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski