Chitinophaga niabensis
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5774 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1N6D0D4|A0A1N6D0D4_9BACT HopJ type III effector protein OS=Chitinophaga niabensis OX=536979 GN=SAMN04488055_0019 PE=4 SV=1
NN1 pKa = 7.68 DD2 pKa = 3.29 TTITQVIHH10 pKa = 5.98 VQDD13 pKa = 3.28 TTAPVFAVAAPADD26 pKa = 3.88 TTVDD30 pKa = 3.98 CNSVPAQPVITATDD44 pKa = 3.54 NCSVTPNITVTRR56 pKa = 11.84 NEE58 pKa = 3.4 VRR60 pKa = 11.84 TNGTCANSYY69 pKa = 10.4 ILTRR73 pKa = 11.84 TWTATDD79 pKa = 3.12 EE80 pKa = 4.66 CGNDD84 pKa = 3.29 TTITQVIHH92 pKa = 5.86 VQDD95 pKa = 3.75 TVAPRR100 pKa = 11.84 FNAIAPADD108 pKa = 3.83 TTVDD112 pKa = 3.98 CNSVPAQPVINATDD126 pKa = 3.42 NCSATGNITITRR138 pKa = 11.84 NEE140 pKa = 3.45 VRR142 pKa = 11.84 TNGTCANTYY151 pKa = 8.84 TLTRR155 pKa = 11.84 TWTAVDD161 pKa = 3.25 EE162 pKa = 4.86 CGNDD166 pKa = 3.16 TTIRR170 pKa = 11.84 QIIHH174 pKa = 5.59 VQDD177 pKa = 3.16 TAAPVFSVVIPADD190 pKa = 3.47 TTVDD194 pKa = 3.97 CNSVPAQAVITATDD208 pKa = 3.33 NCSATGNITITRR220 pKa = 11.84 NEE222 pKa = 3.45 VRR224 pKa = 11.84 TNGTCANSYY233 pKa = 8.89 TLTRR237 pKa = 11.84 TWTAVDD243 pKa = 3.25 EE244 pKa = 4.86 CGNDD248 pKa = 3.34 TTITQVVHH256 pKa = 5.9 VQDD259 pKa = 3.33 TTAPVVTTVIPAARR273 pKa = 11.84 TVDD276 pKa = 3.7 CDD278 pKa = 3.45 AVPVQEE284 pKa = 6.18 DD285 pKa = 3.08 ITATDD290 pKa = 3.14 NCSAVPNISVVKK302 pKa = 10.94 NEE304 pKa = 3.25 VRR306 pKa = 11.84 TNGACANTYY315 pKa = 8.82 TLTRR319 pKa = 11.84 TWVVSDD325 pKa = 3.29 EE326 pKa = 4.65 CGNDD330 pKa = 3.31 TTITQVLTVQDD341 pKa = 3.42 TTAPVVTVVIPATRR355 pKa = 11.84 TVDD358 pKa = 3.41 CDD360 pKa = 3.36 AVPVQEE366 pKa = 6.18 DD367 pKa = 3.17 ITATDD372 pKa = 3.15 NN373 pKa = 3.56
Molecular weight: 39.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.8
IPC2_protein 3.948
IPC_protein 3.973
Toseland 3.745
ProMoST 4.151
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.935
Rodwell 3.795
Grimsley 3.643
Solomon 3.973
Lehninger 3.935
Nozaki 4.088
DTASelect 4.38
Thurlkill 3.795
EMBOSS 3.935
Sillero 4.101
Patrickios 0.718
IPC_peptide 3.961
IPC2_peptide 4.075
IPC2.peptide.svr19 3.969
Protein with the highest isoelectric point:
>tr|A0A1N6DP39|A0A1N6DP39_9BACT Glucose-1-phosphate adenylyltransferase OS=Chitinophaga niabensis OX=536979 GN=SAMN04488055_0952 PE=3 SV=1
MM1 pKa = 7.6 VLIMNWLVLGIILACLNALTLAKK24 pKa = 9.95 FYY26 pKa = 11.45 NPDD29 pKa = 3.39 NPKK32 pKa = 11.35 DD33 pKa = 3.52 MIATVRR39 pKa = 11.84 AKK41 pKa = 10.2 IAEE44 pKa = 4.27 TTLRR48 pKa = 11.84 AWFYY52 pKa = 11.04 FWLTSLRR59 pKa = 11.84 SVSRR63 pKa = 11.84 ISFSRR68 pKa = 11.84 KK69 pKa = 7.92 GLRR72 pKa = 11.84 II73 pKa = 3.63
Molecular weight: 8.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.332
IPC2_protein 9.853
IPC_protein 10.643
Toseland 10.804
ProMoST 10.482
Dawson 10.891
Bjellqvist 10.599
Wikipedia 11.096
Rodwell 11.169
Grimsley 10.935
Solomon 11.008
Lehninger 10.979
Nozaki 10.789
DTASelect 10.599
Thurlkill 10.789
EMBOSS 11.199
Sillero 10.818
Patrickios 10.95
IPC_peptide 11.023
IPC2_peptide 9.619
IPC2.peptide.svr19 8.433
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5774
0
5774
2185197
27
8665
378.5
42.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.015 ± 0.038
0.784 ± 0.012
5.126 ± 0.02
5.541 ± 0.033
4.725 ± 0.024
7.198 ± 0.031
1.883 ± 0.017
6.632 ± 0.024
6.386 ± 0.037
9.413 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.416 ± 0.016
5.289 ± 0.031
4.21 ± 0.02
3.868 ± 0.02
4.48 ± 0.021
6.038 ± 0.027
6.045 ± 0.053
6.464 ± 0.028
1.357 ± 0.014
4.13 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here