Salmonella phage 29485

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V0E5P3|A0A1V0E5P3_9CAUD Uncharacterized protein OS=Salmonella phage 29485 OX=1970747 PE=4 SV=1
MM1 pKa = 7.39ACEE4 pKa = 4.24AGAFTGRR11 pKa = 11.84DD12 pKa = 3.3VVVYY16 pKa = 8.43YY17 pKa = 10.72AISCPEE23 pKa = 3.91VQPSNGDD30 pKa = 3.44YY31 pKa = 10.51TRR33 pKa = 11.84LGMMRR38 pKa = 11.84GKK40 pKa = 7.4TTGAEE45 pKa = 3.97WEE47 pKa = 4.46TADD50 pKa = 3.92ATADD54 pKa = 3.26MSAAFTQEE62 pKa = 3.52NLVTYY67 pKa = 10.57KK68 pKa = 10.79NISFSGDD75 pKa = 2.77GVTRR79 pKa = 11.84KK80 pKa = 10.06EE81 pKa = 4.22DD82 pKa = 3.48VYY84 pKa = 11.51AQNALKK90 pKa = 10.32RR91 pKa = 11.84HH92 pKa = 5.61VYY94 pKa = 10.05NPPAEE99 pKa = 4.4TSNQPYY105 pKa = 9.62VWLKK109 pKa = 10.44IISPNDD115 pKa = 3.09ITEE118 pKa = 4.39GPFMVTSWQDD128 pKa = 3.25EE129 pKa = 4.34APHH132 pKa = 7.19DD133 pKa = 5.3DD134 pKa = 3.43VATWSIEE141 pKa = 3.7ASSAGSVDD149 pKa = 3.34VRR151 pKa = 11.84DD152 pKa = 3.79VGAVITITAQPQNRR166 pKa = 11.84TLTVGDD172 pKa = 4.18ALNLSVSATVSDD184 pKa = 4.79GSALAYY190 pKa = 9.0QWKK193 pKa = 10.03HH194 pKa = 6.23DD195 pKa = 3.89GEE197 pKa = 5.38DD198 pKa = 3.13ISGATSSTYY207 pKa = 9.93TKK209 pKa = 11.0ASVTEE214 pKa = 4.43DD215 pKa = 3.27DD216 pKa = 4.25AGAYY220 pKa = 6.92TCQITSTTAGSLTSGAATVIVNAEE244 pKa = 3.8

Molecular weight: 25.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V0E5M5|A0A1V0E5M5_9CAUD EaA protein OS=Salmonella phage 29485 OX=1970747 PE=4 SV=1
MM1 pKa = 7.31YY2 pKa = 8.23LTLPEE7 pKa = 4.12WNQRR11 pKa = 11.84QPRR14 pKa = 11.84PRR16 pKa = 11.84SLEE19 pKa = 3.86TVRR22 pKa = 11.84RR23 pKa = 11.84WVRR26 pKa = 11.84EE27 pKa = 3.89CRR29 pKa = 11.84ISPPPLKK36 pKa = 10.49DD37 pKa = 2.91GRR39 pKa = 11.84EE40 pKa = 4.06YY41 pKa = 11.08LFHH44 pKa = 6.97EE45 pKa = 4.65NAVKK49 pKa = 10.06IDD51 pKa = 3.83VKK53 pKa = 11.05NKK55 pKa = 6.87PTGRR59 pKa = 11.84LLKK62 pKa = 10.31RR63 pKa = 11.84IRR65 pKa = 11.84DD66 pKa = 3.8GKK68 pKa = 9.38KK69 pKa = 10.12AKK71 pKa = 9.82PP72 pKa = 3.43

Molecular weight: 8.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

87

0

87

15324

37

1056

176.1

19.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.234 ± 0.382

1.227 ± 0.134

5.769 ± 0.198

6.604 ± 0.319

3.113 ± 0.195

7.237 ± 0.331

1.644 ± 0.148

6.056 ± 0.175

5.951 ± 0.284

7.55 ± 0.298

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.969 ± 0.163

4.803 ± 0.286

3.811 ± 0.182

4.137 ± 0.226

6.18 ± 0.309

6.519 ± 0.246

5.455 ± 0.355

6.787 ± 0.277

1.69 ± 0.131

3.263 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski