Estero Real virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Nairoviridae; Orthonairovirus; Estero Real orthonairovirus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A346JIY0|A0A346JIY0_9VIRU Replicase OS=Estero Real virus OX=2170057 PE=4 SV=1
MM1 pKa = 6.51STRR4 pKa = 11.84RR5 pKa = 11.84GDD7 pKa = 3.13YY8 pKa = 10.49DD9 pKa = 3.61YY10 pKa = 11.82ALDD13 pKa = 4.4FPTIKK18 pKa = 10.41DD19 pKa = 3.13WEE21 pKa = 4.07DD22 pKa = 2.98WYY24 pKa = 10.23TEE26 pKa = 4.06FKK28 pKa = 11.51GEE30 pKa = 3.98FAYY33 pKa = 10.86LGSNDD38 pKa = 3.58TNFDD42 pKa = 3.62SFFNGLPDD50 pKa = 3.52LTNFRR55 pKa = 11.84NEE57 pKa = 3.76MEE59 pKa = 4.34VARR62 pKa = 11.84NQSNGLKK69 pKa = 10.53DD70 pKa = 3.99SIVAKK75 pKa = 10.63AIVEE79 pKa = 4.11ATKK82 pKa = 10.67YY83 pKa = 10.27AGPVKK88 pKa = 10.04EE89 pKa = 4.5AAYY92 pKa = 10.01LVNKK96 pKa = 10.39SYY98 pKa = 9.21VTKK101 pKa = 10.33ALKK104 pKa = 10.08WFDD107 pKa = 3.12INRR110 pKa = 11.84NMKK113 pKa = 10.24EE114 pKa = 3.98FTSWDD119 pKa = 3.46ADD121 pKa = 3.61YY122 pKa = 10.76KK123 pKa = 11.46ALMSEE128 pKa = 4.78LPSLDD133 pKa = 3.04QVEE136 pKa = 4.32NYY138 pKa = 9.61KK139 pKa = 10.8LCAQKK144 pKa = 10.21FRR146 pKa = 11.84KK147 pKa = 8.65DD148 pKa = 3.18TEE150 pKa = 4.36LPKK153 pKa = 10.27TPCFRR158 pKa = 11.84VLTGEE163 pKa = 3.88VLTYY167 pKa = 8.46YY168 pKa = 9.56TVNRR172 pKa = 11.84RR173 pKa = 11.84DD174 pKa = 2.98IDD176 pKa = 4.09TINEE180 pKa = 3.85LMKK183 pKa = 10.87DD184 pKa = 3.62MKK186 pKa = 10.53QKK188 pKa = 10.11RR189 pKa = 11.84DD190 pKa = 3.24KK191 pKa = 10.65VAPGGANRR199 pKa = 11.84NKK201 pKa = 10.13EE202 pKa = 3.84QSQANGDD209 pKa = 3.52WVAGFVKK216 pKa = 10.93GEE218 pKa = 3.94ISYY221 pKa = 10.51KK222 pKa = 10.32EE223 pKa = 3.94PPVWGGWLKK232 pKa = 10.89KK233 pKa = 10.36NKK235 pKa = 9.91NPGRR239 pKa = 11.84AMLLCSTALQNAVSRR254 pKa = 11.84RR255 pKa = 11.84LITDD259 pKa = 3.61DD260 pKa = 5.38EE261 pKa = 4.4IALCIQEE268 pKa = 4.4LEE270 pKa = 4.36QMLEE274 pKa = 4.19TPGFDD279 pKa = 4.54GDD281 pKa = 4.05GLTMKK286 pKa = 10.81NIIEE290 pKa = 4.0AMKK293 pKa = 10.78NFEE296 pKa = 4.06QDD298 pKa = 2.93KK299 pKa = 10.91AYY301 pKa = 10.69MNAKK305 pKa = 9.5SAGGVGFQAQSAALDD320 pKa = 3.79SVFSSFYY327 pKa = 10.03WVWKK331 pKa = 10.32SGATVDD337 pKa = 4.89SFPGLAKK344 pKa = 10.22FCHH347 pKa = 6.17EE348 pKa = 4.53LGQRR352 pKa = 11.84SVGKK356 pKa = 10.64VKK358 pKa = 9.65LTEE361 pKa = 4.1VLSNTGWRR369 pKa = 11.84WGKK372 pKa = 10.7GLVNNMTKK380 pKa = 10.39RR381 pKa = 11.84FNIDD385 pKa = 4.01PIHH388 pKa = 5.78MHH390 pKa = 6.54PAVLTAGRR398 pKa = 11.84VITEE402 pKa = 3.72MTACFGVVPPHH413 pKa = 6.41EE414 pKa = 4.62PYY416 pKa = 10.35KK417 pKa = 10.6AAEE420 pKa = 4.54GCGSLRR426 pKa = 11.84NLLNLEE432 pKa = 3.98TGVRR436 pKa = 11.84NVCASAICDD445 pKa = 3.59LFNVYY450 pKa = 9.9KK451 pKa = 10.31QGYY454 pKa = 7.54PRR456 pKa = 11.84YY457 pKa = 8.71EE458 pKa = 4.0DD459 pKa = 5.26LIVPMEE465 pKa = 4.31HH466 pKa = 6.71MLHH469 pKa = 6.34QSFLGKK475 pKa = 10.35ASPYY479 pKa = 10.3QYY481 pKa = 11.39AGLLDD486 pKa = 3.87GDD488 pKa = 3.99AFKK491 pKa = 10.95IEE493 pKa = 4.14IVPGQGLRR501 pKa = 11.84GSEE504 pKa = 4.07VV505 pKa = 2.89

Molecular weight:
56.77 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A346JIY6|A0A346JIY6_9VIRU Glycoprotein G2 OS=Estero Real virus OX=2170057 PE=4 SV=1
MM1 pKa = 7.92DD2 pKa = 4.14RR3 pKa = 11.84HH4 pKa = 6.2DD5 pKa = 4.21HH6 pKa = 7.07LDD8 pKa = 3.42RR9 pKa = 11.84ALLLFVCLLLFYY21 pKa = 11.04LPFILKK27 pKa = 10.19DD28 pKa = 3.28MCNCGLTQTCSVSHH42 pKa = 7.37DD43 pKa = 3.18LMFYY47 pKa = 9.72RR48 pKa = 11.84FAMAEE53 pKa = 4.08EE54 pKa = 4.86LGPTPTNPAEE64 pKa = 4.38KK65 pKa = 10.31ASGASPTNHH74 pKa = 5.73VPLTTKK80 pKa = 10.4GQVIMSRR87 pKa = 11.84DD88 pKa = 3.36QTVSLEE94 pKa = 4.08EE95 pKa = 4.08TLPPEE100 pKa = 4.49APSQVRR106 pKa = 11.84FTIEE110 pKa = 3.29RR111 pKa = 11.84SRR113 pKa = 11.84RR114 pKa = 11.84MIEE117 pKa = 3.67NRR119 pKa = 11.84GLRR122 pKa = 11.84EE123 pKa = 4.09EE124 pKa = 4.94LDD126 pKa = 3.54MVLDD130 pKa = 3.98KK131 pKa = 11.1AVKK134 pKa = 10.21QLDD137 pKa = 3.69NYY139 pKa = 11.1AVIISKK145 pKa = 8.47PTLPKK150 pKa = 9.98RR151 pKa = 11.84EE152 pKa = 4.06SSVEE156 pKa = 3.7VDD158 pKa = 3.42STHH161 pKa = 7.45LEE163 pKa = 4.03LAISADD169 pKa = 4.4LDD171 pKa = 4.36DD172 pKa = 5.31EE173 pKa = 5.53DD174 pKa = 5.85LFEE177 pKa = 7.04DD178 pKa = 4.79LMEE181 pKa = 4.49EE182 pKa = 3.75SSAYY186 pKa = 10.29VFAASKK192 pKa = 9.26DD193 pKa = 3.64TSKK196 pKa = 11.34LKK198 pKa = 10.53IVVKK202 pKa = 10.72KK203 pKa = 10.53EE204 pKa = 3.71NNTTALVNRR213 pKa = 11.84NITFGYY219 pKa = 9.3LQGGDD224 pKa = 3.93GMYY227 pKa = 10.72LLCHH231 pKa = 5.61EE232 pKa = 4.98RR233 pKa = 11.84AWDD236 pKa = 3.58EE237 pKa = 4.54DD238 pKa = 4.24SNNLVNMSVVKK249 pKa = 9.88QGCDD253 pKa = 2.64RR254 pKa = 11.84GYY256 pKa = 9.19GCCGVEE262 pKa = 4.33EE263 pKa = 4.42PQVIWVEE270 pKa = 3.99DD271 pKa = 3.61LDD273 pKa = 4.17YY274 pKa = 11.24PNLFMSSPKK283 pKa = 10.33GSNFTIINYY292 pKa = 6.58LTSYY296 pKa = 11.14RR297 pKa = 11.84LFSYY301 pKa = 9.45QGCSMRR307 pKa = 11.84IEE309 pKa = 4.51AGGCLMKK316 pKa = 10.68TSVDD320 pKa = 3.76NNWNPFYY327 pKa = 10.3MYY329 pKa = 10.3YY330 pKa = 10.0NEE332 pKa = 5.22KK333 pKa = 9.72ILPVAHH339 pKa = 6.59ILVPRR344 pKa = 11.84TKK346 pKa = 10.82EE347 pKa = 3.9NINCRR352 pKa = 11.84IRR354 pKa = 11.84MCAIMHH360 pKa = 5.77GRR362 pKa = 11.84EE363 pKa = 4.36SKK365 pKa = 11.06NGIHH369 pKa = 5.76TSKK372 pKa = 10.43FRR374 pKa = 11.84TVEE377 pKa = 3.78VKK379 pKa = 9.99IFKK382 pKa = 9.4GQEE385 pKa = 3.23VRR387 pKa = 11.84TRR389 pKa = 11.84KK390 pKa = 9.68LLSVDD395 pKa = 3.65PRR397 pKa = 11.84ISSYY401 pKa = 10.21KK402 pKa = 8.28WQHH405 pKa = 5.13SCNTRR410 pKa = 11.84TMMFPYY416 pKa = 9.98QKK418 pKa = 10.75SLVHH422 pKa = 5.06TVGRR426 pKa = 11.84SIAGDD431 pKa = 3.01KK432 pKa = 7.99MTFCNGTRR440 pKa = 11.84HH441 pKa = 5.78TSLEE445 pKa = 3.75LGGPYY450 pKa = 9.95NCYY453 pKa = 9.27TVGHH457 pKa = 5.55QQTLFQCPGLKK468 pKa = 9.32RR469 pKa = 11.84HH470 pKa = 6.38RR471 pKa = 11.84KK472 pKa = 8.37GEE474 pKa = 4.13KK475 pKa = 9.42VDD477 pKa = 3.86VNCTVDD483 pKa = 3.99LHH485 pKa = 5.52PHH487 pKa = 5.93KK488 pKa = 9.88CNHH491 pKa = 6.99GICIKK496 pKa = 10.51IKK498 pKa = 9.13MEE500 pKa = 3.81GSGFVKK506 pKa = 10.7LSGTSWSAIKK516 pKa = 10.61KK517 pKa = 9.76CDD519 pKa = 3.84KK520 pKa = 10.32EE521 pKa = 4.26CNAYY525 pKa = 10.19FGQGDD530 pKa = 4.01NMVVQCPDD538 pKa = 2.96GKK540 pKa = 10.2LHH542 pKa = 6.55HH543 pKa = 6.31VHH545 pKa = 7.18ANRR548 pKa = 11.84IDD550 pKa = 3.79FDD552 pKa = 4.7CPFVDD557 pKa = 3.91YY558 pKa = 10.48TKK560 pKa = 10.86GASLYY565 pKa = 9.67VCRR568 pKa = 11.84ATSRR572 pKa = 11.84PKK574 pKa = 10.56LLYY577 pKa = 10.68ALIFWAFGGVPLMITVLTVLRR598 pKa = 11.84FICYY602 pKa = 9.3LASLVCIYY610 pKa = 10.47LKK612 pKa = 10.07SVRR615 pKa = 11.84DD616 pKa = 3.67RR617 pKa = 11.84SRR619 pKa = 11.84KK620 pKa = 8.14HH621 pKa = 6.92CEE623 pKa = 3.63GCGCFVNSCYY633 pKa = 9.92EE634 pKa = 3.98WQRR637 pKa = 11.84HH638 pKa = 4.57EE639 pKa = 3.98LCKK642 pKa = 10.89NGSCPFCKK650 pKa = 9.91NRR652 pKa = 11.84YY653 pKa = 8.28SVSGLLEE660 pKa = 4.26HH661 pKa = 7.35APKK664 pKa = 10.76CLDD667 pKa = 3.29RR668 pKa = 11.84KK669 pKa = 10.03RR670 pKa = 11.84VEE672 pKa = 4.17SSDD675 pKa = 4.01LEE677 pKa = 4.56VVNLEE682 pKa = 3.99RR683 pKa = 11.84LPRR686 pKa = 11.84PLRR689 pKa = 11.84QLGTLCSKK697 pKa = 10.84ARR699 pKa = 11.84GRR701 pKa = 11.84TVKK704 pKa = 10.66ALWVLIMIISFMMVISPASSTMIEE728 pKa = 4.68FKK730 pKa = 11.04QNGLWTEE737 pKa = 3.89EE738 pKa = 4.11LEE740 pKa = 4.18EE741 pKa = 4.29VEE743 pKa = 4.41ICRR746 pKa = 11.84SEE748 pKa = 4.87CEE750 pKa = 4.0FLEE753 pKa = 4.66DD754 pKa = 5.08KK755 pKa = 10.74CRR757 pKa = 11.84CLAKK761 pKa = 10.36HH762 pKa = 5.5EE763 pKa = 4.21QLSRR767 pKa = 11.84TLLALMPKK775 pKa = 8.42EE776 pKa = 3.99TSTYY780 pKa = 9.1TVDD783 pKa = 3.84VQAPWGTVRR792 pKa = 11.84IGDD795 pKa = 4.18TIKK798 pKa = 10.49PRR800 pKa = 11.84YY801 pKa = 8.0SPKK804 pKa = 10.41AITMSWTTVDD814 pKa = 3.71SLPDD818 pKa = 3.46SEE820 pKa = 5.65HH821 pKa = 6.68IEE823 pKa = 3.72LSGRR827 pKa = 11.84SEE829 pKa = 4.18TYY831 pKa = 10.08IPLLEE836 pKa = 4.24KK837 pKa = 10.26TGITWTLSDD846 pKa = 3.75HH847 pKa = 6.23SAAEE851 pKa = 3.89KK852 pKa = 9.04RR853 pKa = 11.84TLVINILDD861 pKa = 3.84FTQVYY866 pKa = 6.6EE867 pKa = 4.22TRR869 pKa = 11.84FLYY872 pKa = 9.28LTGDD876 pKa = 3.54RR877 pKa = 11.84TVGNWMHH884 pKa = 6.07GTCTGDD890 pKa = 3.97CPDD893 pKa = 4.16KK894 pKa = 11.05CGCDD898 pKa = 3.4TSSCTYY904 pKa = 10.76ARR906 pKa = 11.84WRR908 pKa = 11.84YY909 pKa = 8.73SRR911 pKa = 11.84NWHH914 pKa = 6.64CNPGWCWAMDD924 pKa = 3.91SGCTCCALDD933 pKa = 3.36INKK936 pKa = 9.32FYY938 pKa = 10.93TDD940 pKa = 3.09WLVSKK945 pKa = 9.75WAVTYY950 pKa = 10.7KK951 pKa = 9.72GTEE954 pKa = 3.7VLACVEE960 pKa = 4.28YY961 pKa = 10.82NSDD964 pKa = 3.54YY965 pKa = 10.74RR966 pKa = 11.84SCDD969 pKa = 3.18VFHH972 pKa = 7.94AGMTISHH979 pKa = 6.86GPFKK983 pKa = 11.11VQFSEE988 pKa = 4.36PKK990 pKa = 10.55NIGKK994 pKa = 9.72KK995 pKa = 10.21LPEE998 pKa = 4.67TILLVHH1004 pKa = 6.27GAKK1007 pKa = 10.19GASDD1011 pKa = 4.01GLDD1014 pKa = 2.7LHH1016 pKa = 6.87MIHH1019 pKa = 6.75EE1020 pKa = 4.75VTSGVQACRR1029 pKa = 11.84LQSCTHH1035 pKa = 6.3GSVGDD1040 pKa = 3.86MQILNLNDD1048 pKa = 3.62MIGNDD1053 pKa = 3.91VYY1055 pKa = 11.06FEE1057 pKa = 4.76HH1058 pKa = 7.11YY1059 pKa = 7.56FTNEE1063 pKa = 3.46QKK1065 pKa = 10.74KK1066 pKa = 10.25RR1067 pKa = 11.84GAPKK1071 pKa = 10.43ADD1073 pKa = 3.61GEE1075 pKa = 4.3DD1076 pKa = 3.45DD1077 pKa = 4.17RR1078 pKa = 11.84VWMSWDD1084 pKa = 3.16GVVQRR1089 pKa = 11.84YY1090 pKa = 8.7HH1091 pKa = 6.71CVATKK1096 pKa = 10.05WPDD1099 pKa = 3.35CEE1101 pKa = 3.97ASGIVTRR1108 pKa = 11.84NHH1110 pKa = 6.89ASFQNLIKK1118 pKa = 10.17TSFNYY1123 pKa = 8.81SAEE1126 pKa = 4.3YY1127 pKa = 10.0FFHH1130 pKa = 7.09ALHH1133 pKa = 6.3VSLNQSVPTLEE1144 pKa = 4.73LEE1146 pKa = 4.38ARR1148 pKa = 11.84PHH1150 pKa = 5.71EE1151 pKa = 4.33MAGGLDD1157 pKa = 3.15IFLEE1161 pKa = 4.24VDD1163 pKa = 3.04KK1164 pKa = 11.47LRR1166 pKa = 11.84LKK1168 pKa = 10.67KK1169 pKa = 10.45KK1170 pKa = 9.42EE1171 pKa = 3.86ASLTDD1176 pKa = 3.59LQLLVEE1182 pKa = 4.49SCEE1185 pKa = 4.02GCYY1188 pKa = 10.84GCVSGIDD1195 pKa = 3.63CTVSIVMSGISEE1207 pKa = 4.23IYY1209 pKa = 9.92FHH1211 pKa = 6.88LKK1213 pKa = 10.07SKK1215 pKa = 9.53TEE1217 pKa = 3.8HH1218 pKa = 5.85FVISSSSIPVTDD1230 pKa = 3.63QEE1232 pKa = 4.59KK1233 pKa = 10.69KK1234 pKa = 9.17RR1235 pKa = 11.84ATVKK1239 pKa = 10.62GFTPIPLKK1247 pKa = 9.77QVCLEE1252 pKa = 3.96IEE1254 pKa = 4.07EE1255 pKa = 4.81SGLCKK1260 pKa = 10.22KK1261 pKa = 10.68CSPTVSCIPVSLSEE1275 pKa = 4.23PKK1277 pKa = 10.43AVALEE1282 pKa = 4.13HH1283 pKa = 7.06RR1284 pKa = 11.84SMLHH1288 pKa = 5.57VQLKK1292 pKa = 10.12DD1293 pKa = 2.99NCTSVISCIGGGLQGFLSNLSALFGSFFGPWFTGIFMTALFFIVAVLTVLYY1344 pKa = 10.39GPTAFRR1350 pKa = 11.84LAKK1353 pKa = 9.73VVCCSKK1359 pKa = 10.57SRR1361 pKa = 11.84KK1362 pKa = 9.15LKK1364 pKa = 9.41SARR1367 pKa = 11.84GILKK1371 pKa = 10.25LYY1373 pKa = 10.37QKK1375 pKa = 10.86AEE1377 pKa = 4.07KK1378 pKa = 10.11EE1379 pKa = 3.94RR1380 pKa = 11.84LLSGKK1385 pKa = 9.53GQAVDD1390 pKa = 3.66SDD1392 pKa = 4.19DD1393 pKa = 3.53LATYY1397 pKa = 9.22FKK1399 pKa = 10.64KK1400 pKa = 10.79DD1401 pKa = 3.02KK1402 pKa = 11.08

Molecular weight:
158.01 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

5832

505

3925

1944.0

220.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.75 ± 0.587

2.898 ± 0.739

5.556 ± 0.166

6.842 ± 0.402

4.27 ± 0.113

5.195 ± 0.831

2.16 ± 0.379

6.241 ± 0.831

7.51 ± 0.357

10.219 ± 0.537

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.401 ± 0.247

4.63 ± 0.364

3.481 ± 0.231

3.429 ± 0.308

4.733 ± 0.129

8.762 ± 0.765

6.07 ± 0.157

6.361 ± 0.309

1.286 ± 0.256

3.206 ± 0.166

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski