Canada goose coronavirus
Average proteome isoelectric point is 7.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 16 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6FUX7|A0A4D6FUX7_9GAMC Envelope protein OS=Canada goose coronavirus OX=2569586 PE=4 SV=1
MM1 pKa = 7.68 SDD3 pKa = 4.81 DD4 pKa = 4.13 SSCCIHH10 pKa = 6.43 NPCLSEE16 pKa = 3.51 WCVYY20 pKa = 10.29 CNPPDD25 pKa = 3.76 EE26 pKa = 5.27 DD27 pKa = 3.82 VPLHH31 pKa = 5.52 VLSFIINSQQEE42 pKa = 4.4 LLEE45 pKa = 5.16 LIDD48 pKa = 6.0 LLFEE52 pKa = 4.37 SS53 pKa = 4.96
Molecular weight: 6.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.935
IPC2_protein 3.694
IPC_protein 3.541
Toseland 3.376
ProMoST 3.745
Dawson 3.554
Bjellqvist 3.719
Wikipedia 3.516
Rodwell 3.401
Grimsley 3.3
Solomon 3.49
Lehninger 3.439
Nozaki 3.706
DTASelect 3.834
Thurlkill 3.465
EMBOSS 3.528
Sillero 3.668
Patrickios 0.006
IPC_peptide 3.49
IPC2_peptide 3.63
IPC2.peptide.svr19 3.665
Protein with the highest isoelectric point:
>tr|A0A4D6FTX3|A0A4D6FTX3_9GAMC ORF3 OS=Canada goose coronavirus OX=2569586 PE=4 SV=1
MM1 pKa = 7.5 NNPFATATARR11 pKa = 11.84 KK12 pKa = 9.54 ARR14 pKa = 11.84 VVCLNRR20 pKa = 11.84 GARR23 pKa = 11.84 SVYY26 pKa = 9.97 FLNKK30 pKa = 9.38 QGLPVPCPHH39 pKa = 6.77 CTTLVVRR46 pKa = 11.84 GLLCEE51 pKa = 3.94 EE52 pKa = 4.7 HH53 pKa = 6.75 QFDD56 pKa = 4.55 DD57 pKa = 4.09 NIYY60 pKa = 10.24 IGWHH64 pKa = 5.92 PEE66 pKa = 3.33 GTLEE70 pKa = 4.56 HH71 pKa = 6.68 ARR73 pKa = 11.84 TRR75 pKa = 11.84 VGNRR79 pKa = 11.84 MLQHH83 pKa = 6.91 RR84 pKa = 11.84 FF85 pKa = 3.16
Molecular weight: 9.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.977
IPC2_protein 8.639
IPC_protein 8.873
Toseland 8.931
ProMoST 9.048
Dawson 9.311
Bjellqvist 9.487
Wikipedia 9.385
Rodwell 9.341
Grimsley 8.873
Solomon 9.589
Lehninger 9.575
Nozaki 9.721
DTASelect 9.268
Thurlkill 9.326
EMBOSS 9.487
Sillero 9.589
Patrickios 4.978
IPC_peptide 9.575
IPC2_peptide 9.121
IPC2.peptide.svr19 7.938
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
16
0
16
13107
53
6510
819.2
91.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.905 ± 0.538
3.449 ± 0.212
5.547 ± 0.274
3.716 ± 0.254
5.936 ± 0.269
6.058 ± 0.323
1.747 ± 0.207
4.738 ± 0.323
5.325 ± 0.398
9.163 ± 0.409
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.915 ± 0.104
5.562 ± 0.218
3.7 ± 0.367
3.441 ± 0.587
3.464 ± 0.526
6.798 ± 0.325
5.638 ± 0.519
10.513 ± 0.933
1.381 ± 0.078
5.005 ± 0.39
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here