Salmonella phage SSE121
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 242 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K4I5N2|K4I5N2_9CAUD Uncharacterized protein OS=Salmonella phage SSE121 OX=1204529 PE=4 SV=1
MM1 pKa = 7.31 QNFITISNNDD11 pKa = 3.59 KK12 pKa = 10.35 LQSLIGILNDD22 pKa = 3.04 VSPFIVKK29 pKa = 9.81 NLYY32 pKa = 10.32 AHH34 pKa = 7.18 LKK36 pKa = 8.49 NVKK39 pKa = 8.79 PAIWSAYY46 pKa = 10.06 CHH48 pKa = 6.42 DD49 pKa = 4.04 QDD51 pKa = 4.31 EE52 pKa = 4.55 SAAAYY57 pKa = 8.42 MLEE60 pKa = 3.89 MLKK63 pKa = 10.95 DD64 pKa = 3.66 SMQFCLSQSVINEE77 pKa = 3.59 VDD79 pKa = 3.36 SRR81 pKa = 11.84 LSLAMEE87 pKa = 4.18 YY88 pKa = 9.95 QAQAVYY94 pKa = 10.27 NQLDD98 pKa = 3.47 IEE100 pKa = 4.32 EE101 pKa = 4.51 QQDD104 pKa = 3.61 QDD106 pKa = 4.63 LFWDD110 pKa = 3.89 RR111 pKa = 11.84 ADD113 pKa = 3.72 AQSAFDD119 pKa = 5.22 DD120 pKa = 4.07 RR121 pKa = 11.84 LAMYY125 pKa = 10.55 ANEE128 pKa = 4.25 YY129 pKa = 10.16
Molecular weight: 14.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.841
IPC2_protein 4.101
IPC_protein 4.062
Toseland 3.846
ProMoST 4.215
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.986
Rodwell 3.884
Grimsley 3.757
Solomon 4.037
Lehninger 3.999
Nozaki 4.164
DTASelect 4.406
Thurlkill 3.897
EMBOSS 3.999
Sillero 4.177
Patrickios 1.964
IPC_peptide 4.037
IPC2_peptide 4.151
IPC2.peptide.svr19 4.068
Protein with the highest isoelectric point:
>tr|K4I3L5|K4I3L5_9CAUD Uncharacterized protein OS=Salmonella phage SSE121 OX=1204529 PE=4 SV=1
MM1 pKa = 7.53 KK2 pKa = 10.48 NYY4 pKa = 10.0 FNSRR8 pKa = 11.84 ALARR12 pKa = 11.84 TAAKK16 pKa = 10.68 ANGGKK21 pKa = 10.55 VIDD24 pKa = 4.7 NGTAAPAGKK33 pKa = 9.06 RR34 pKa = 11.84 WQVIPGVMVEE44 pKa = 4.99 AIQDD48 pKa = 3.72 TADD51 pKa = 2.94 QRR53 pKa = 11.84 AAMEE57 pKa = 4.28 SAPALVVTLEE67 pKa = 3.85 IKK69 pKa = 10.2 AGYY72 pKa = 9.74 RR73 pKa = 11.84 NRR75 pKa = 11.84 QAARR79 pKa = 11.84 VSYY82 pKa = 10.98 VHH84 pKa = 7.27 DD85 pKa = 4.8 ANGKK89 pKa = 8.24 PIPVTHH95 pKa = 6.8 KK96 pKa = 9.92 RR97 pKa = 11.84 SQIAARR103 pKa = 11.84 LAAHH107 pKa = 6.73 MARR110 pKa = 4.5
Molecular weight: 11.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.314
IPC2_protein 9.897
IPC_protein 10.774
Toseland 10.95
ProMoST 11.023
Dawson 11.023
Bjellqvist 10.745
Wikipedia 11.257
Rodwell 11.257
Grimsley 11.067
Solomon 11.184
Lehninger 11.14
Nozaki 10.921
DTASelect 10.745
Thurlkill 10.935
EMBOSS 11.359
Sillero 10.95
Patrickios 11.008
IPC_peptide 11.184
IPC2_peptide 9.575
IPC2.peptide.svr19 8.611
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
242
0
242
44064
27
929
182.1
20.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.133 ± 0.228
1.262 ± 0.097
6.631 ± 0.142
6.658 ± 0.221
4.237 ± 0.121
7.466 ± 0.299
1.847 ± 0.106
5.721 ± 0.108
6.854 ± 0.243
7.875 ± 0.156
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.914 ± 0.095
4.777 ± 0.15
3.701 ± 0.141
3.529 ± 0.133
4.827 ± 0.143
5.846 ± 0.151
5.857 ± 0.221
7.174 ± 0.135
1.743 ± 0.093
3.947 ± 0.117
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here