Nocardioides szechwanensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides

Average proteome isoelectric point is 5.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4039 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H0CHA8|A0A1H0CHA8_9ACTN Putative Holin-X holin superfamily III OS=Nocardioides szechwanensis OX=1005944 GN=SAMN05192576_2425 PE=4 SV=1
MM1 pKa = 7.92RR2 pKa = 11.84RR3 pKa = 11.84QLQFAAVAATCLAAGACGSDD23 pKa = 3.84EE24 pKa = 4.53EE25 pKa = 4.71PVAAADD31 pKa = 4.63DD32 pKa = 4.56APSHH36 pKa = 6.37NLTAHH41 pKa = 6.94PALPDD46 pKa = 3.95APHH49 pKa = 5.79WTYY52 pKa = 11.78DD53 pKa = 3.68EE54 pKa = 4.37APEE57 pKa = 4.33WGDD60 pKa = 4.61LDD62 pKa = 3.84EE63 pKa = 5.66QYY65 pKa = 11.12AACGTGEE72 pKa = 4.07RR73 pKa = 11.84QSPVDD78 pKa = 3.51LAGAADD84 pKa = 4.62EE85 pKa = 5.39DD86 pKa = 4.41LADD89 pKa = 4.5PVLDD93 pKa = 3.75YY94 pKa = 11.38QPFEE98 pKa = 4.25LTVTDD103 pKa = 4.0TDD105 pKa = 3.46HH106 pKa = 7.07SIQVGYY112 pKa = 8.13EE113 pKa = 3.86TGSTLTLDD121 pKa = 3.66DD122 pKa = 3.8AAYY125 pKa = 10.32EE126 pKa = 3.9LVQLHH131 pKa = 5.6FHH133 pKa = 6.76APSEE137 pKa = 4.15HH138 pKa = 6.48TIDD141 pKa = 4.09GKK143 pKa = 9.94PAAAEE148 pKa = 3.61IHH150 pKa = 6.13FVHH153 pKa = 7.08GDD155 pKa = 3.07HH156 pKa = 7.66DD157 pKa = 4.5GPFVVLGVLVEE168 pKa = 4.32EE169 pKa = 5.39GPPEE173 pKa = 3.88AAMNDD178 pKa = 3.94VLDD181 pKa = 4.45HH182 pKa = 6.62LPVGTGDD189 pKa = 3.58ADD191 pKa = 3.41AGTFFDD197 pKa = 5.04AAEE200 pKa = 4.56LLPDD204 pKa = 3.35SLTAYY209 pKa = 9.91RR210 pKa = 11.84YY211 pKa = 8.79EE212 pKa = 4.65GSLTTPPCTEE222 pKa = 3.81DD223 pKa = 3.57VTWLVLDD230 pKa = 4.93EE231 pKa = 4.38PVTWSAEE238 pKa = 3.69QLARR242 pKa = 11.84LTARR246 pKa = 11.84YY247 pKa = 8.17DD248 pKa = 3.46ANSRR252 pKa = 11.84PLQDD256 pKa = 3.34LHH258 pKa = 8.48DD259 pKa = 5.37RR260 pKa = 11.84DD261 pKa = 4.68LLLDD265 pKa = 3.46QDD267 pKa = 3.99

Molecular weight: 28.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H0BYT2|A0A1H0BYT2_9ACTN UDP-glucose 4-epimerase OS=Nocardioides szechwanensis OX=1005944 GN=SAMN05192576_2273 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.24TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4039

0

4039

1303589

24

2147

322.8

34.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.648 ± 0.052

0.746 ± 0.011

6.625 ± 0.034

5.817 ± 0.039

2.865 ± 0.024

9.143 ± 0.038

2.177 ± 0.019

3.553 ± 0.027

2.074 ± 0.025

10.309 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.907 ± 0.017

1.852 ± 0.02

5.606 ± 0.032

2.764 ± 0.017

7.319 ± 0.042

5.392 ± 0.022

6.242 ± 0.035

9.422 ± 0.036

1.512 ± 0.013

2.027 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski