Beihai shrimp virus 3
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KPD6|A0A1L3KPD6_9VIRU Uncharacterized protein OS=Beihai shrimp virus 3 OX=1922669 PE=4 SV=1
MM1 pKa = 7.9 AEE3 pKa = 3.9 TFLAILLLLLWCSPLPVLAKK23 pKa = 10.28 GYY25 pKa = 9.14 SPHH28 pKa = 5.11 VTADD32 pKa = 3.07 KK33 pKa = 9.66 EE34 pKa = 4.38 RR35 pKa = 11.84 NMVRR39 pKa = 11.84 VGAFTPRR46 pKa = 11.84 SEE48 pKa = 4.37 CTVTIKK54 pKa = 10.78 PSGPCAWTADD64 pKa = 3.89 GVGHH68 pKa = 6.95 FDD70 pKa = 3.54 GKK72 pKa = 10.21 NVSWCPAYY80 pKa = 9.77 PGNCDD85 pKa = 3.07 LRR87 pKa = 11.84 KK88 pKa = 7.15 YY89 pKa = 9.59 TEE91 pKa = 4.12 MNGMRR96 pKa = 11.84 TWSGCKK102 pKa = 9.56 RR103 pKa = 11.84 YY104 pKa = 9.99 AVGNKK109 pKa = 9.36 VGMCDD114 pKa = 3.78 VEE116 pKa = 5.74 GEE118 pKa = 4.58 TFDD121 pKa = 5.18 HH122 pKa = 6.63 MEE124 pKa = 4.41 GVCKK128 pKa = 10.41 SVTEE132 pKa = 4.23 MEE134 pKa = 4.62 NDD136 pKa = 3.31 PCLLFWGDD144 pKa = 3.25 GTANEE149 pKa = 4.15 ILAATEE155 pKa = 3.88 QLEE158 pKa = 4.3 PIYY161 pKa = 10.57 TGSNYY166 pKa = 10.67 KK167 pKa = 9.7 MFVIEE172 pKa = 4.05 WKK174 pKa = 9.03 KK175 pKa = 10.78 VKK177 pKa = 8.92 WQFHH181 pKa = 5.86 ISDD184 pKa = 3.95 GGSLFRR190 pKa = 11.84 MCRR193 pKa = 11.84 TAWDD197 pKa = 4.3 HH198 pKa = 7.15 SCGTPCCRR206 pKa = 11.84 AVPEE210 pKa = 4.42 GEE212 pKa = 4.21 RR213 pKa = 11.84 LVCLITDD220 pKa = 4.02 DD221 pKa = 5.81 LYY223 pKa = 11.43 EE224 pKa = 4.28 KK225 pKa = 10.33 CQYY228 pKa = 10.41 QIRR231 pKa = 11.84 QEE233 pKa = 4.0 DD234 pKa = 4.13 EE235 pKa = 3.57 KK236 pKa = 11.03 LQRR239 pKa = 11.84 YY240 pKa = 8.72 PEE242 pKa = 4.75 DD243 pKa = 3.67 LDD245 pKa = 3.46 KK246 pKa = 11.35 TEE248 pKa = 4.25 PSGYY252 pKa = 10.53 LPVDD256 pKa = 4.6 LDD258 pKa = 3.94 TKK260 pKa = 10.81 DD261 pKa = 3.95 LQHH264 pKa = 6.75 HH265 pKa = 5.82 SEE267 pKa = 4.15 VHH269 pKa = 5.68 FSEE272 pKa = 5.25 LDD274 pKa = 3.45 EE275 pKa = 5.19 LVDD278 pKa = 5.6 SEE280 pKa = 5.13 ISCQRR285 pKa = 11.84 PPLICGSRR293 pKa = 11.84 RR294 pKa = 11.84 YY295 pKa = 8.22 TDD297 pKa = 3.3 YY298 pKa = 10.94 KK299 pKa = 9.69 VSEE302 pKa = 4.37 RR303 pKa = 11.84 EE304 pKa = 3.5 VWAPSTPADD313 pKa = 3.92 SPNGAFKK320 pKa = 9.81 CTPTDD325 pKa = 3.56 PQTTSVEE332 pKa = 4.16 CHH334 pKa = 5.57 NFCLVKK340 pKa = 10.77 ASGLCSGSEE349 pKa = 4.05 SFGKK353 pKa = 10.05 FFKK356 pKa = 10.53 IGGGFSCHH364 pKa = 6.89 CDD366 pKa = 2.65 WVTKK370 pKa = 10.53 ISTEE374 pKa = 4.03 TIYY377 pKa = 10.74 TVGTSIYY384 pKa = 9.68 YY385 pKa = 10.31 GEE387 pKa = 4.66 HH388 pKa = 6.44 EE389 pKa = 4.68 CTVGAACAAANNTMRR404 pKa = 11.84 RR405 pKa = 11.84 IGRR408 pKa = 11.84 RR409 pKa = 11.84 LHH411 pKa = 6.6 GVTEE415 pKa = 3.98 EE416 pKa = 4.49 LKK418 pKa = 11.0 CKK420 pKa = 9.9 STDD423 pKa = 3.37 GQVDD427 pKa = 3.73 CGIAEE432 pKa = 4.41 CMFLVSAGNSSAVGRR447 pKa = 11.84 AGDD450 pKa = 3.79 QIKK453 pKa = 10.78 LSVGLRR459 pKa = 11.84 DD460 pKa = 3.7 RR461 pKa = 11.84 LRR463 pKa = 11.84 GYY465 pKa = 9.85 YY466 pKa = 10.17 LQVKK470 pKa = 7.62 LLCDD474 pKa = 3.83 TPVMAGLKK482 pKa = 7.95 TLKK485 pKa = 10.19 KK486 pKa = 10.1 QRR488 pKa = 11.84 NEE490 pKa = 3.55 NHH492 pKa = 5.37 GSYY495 pKa = 10.67 RR496 pKa = 11.84 LFVPPEE502 pKa = 4.03 EE503 pKa = 5.08 LCDD506 pKa = 3.93 CLGLRR511 pKa = 11.84 SLLLDD516 pKa = 3.56 DD517 pKa = 4.85 CRR519 pKa = 11.84 SLRR522 pKa = 11.84 SAIVAMGTSAVVEE535 pKa = 4.56 FLAILWCIVLIVWILSVVKK554 pKa = 10.51 KK555 pKa = 9.86 FLYY558 pKa = 10.25 RR559 pKa = 11.84 AGFLLDD565 pKa = 3.51 GRR567 pKa = 11.84 SVSNEE572 pKa = 3.45 LLEE575 pKa = 4.32 CRR577 pKa = 11.84 EE578 pKa = 4.1 TGEE581 pKa = 5.06 LRR583 pKa = 11.84 DD584 pKa = 3.64 EE585 pKa = 4.01 QVRR588 pKa = 11.84 IIKK591 pKa = 10.34 LYY593 pKa = 10.88 FEE595 pKa = 4.9 DD596 pKa = 6.23 AITKK600 pKa = 10.4 HH601 pKa = 5.63 MFCSLGWWTRR611 pKa = 11.84 RR612 pKa = 11.84 QQRR615 pKa = 11.84 ALKK618 pKa = 9.42 KK619 pKa = 9.95 VCLSYY624 pKa = 11.15 YY625 pKa = 10.21 LYY627 pKa = 10.9 LIIEE631 pKa = 5.17 GYY633 pKa = 9.86 GKK635 pKa = 10.18 NDD637 pKa = 2.27 AWQRR641 pKa = 11.84 SCKK644 pKa = 9.25 IWKK647 pKa = 8.65 QAAAGNEE654 pKa = 4.19 LKK656 pKa = 11.11 DD657 pKa = 3.91 EE658 pKa = 4.17 VLKK661 pKa = 11.24 DD662 pKa = 3.68 LVTSGSRR669 pKa = 11.84 KK670 pKa = 8.95 IGARR674 pKa = 11.84 SDD676 pKa = 3.51 QKK678 pKa = 9.89 TLNPASLSGVLVTLVYY694 pKa = 10.77 CFTLMRR700 pKa = 11.84 LVGGSCEE707 pKa = 4.22 TGSVDD712 pKa = 4.15 VSSSVCTMASEE723 pKa = 4.53 DD724 pKa = 3.86 TMSCSVKK731 pKa = 8.32 TTILSTIDD739 pKa = 3.31 VVGKK743 pKa = 7.39 STCFNLQNKK752 pKa = 5.87 TTGDD756 pKa = 2.79 IVGQVEE762 pKa = 4.46 VEE764 pKa = 3.85 YY765 pKa = 11.69 SMLEE769 pKa = 4.22 RR770 pKa = 11.84 AWSLNNLYY778 pKa = 8.69 YY779 pKa = 10.05 TGHH782 pKa = 6.68 WSLMTRR788 pKa = 11.84 EE789 pKa = 4.97 DD790 pKa = 3.53 SSCYY794 pKa = 9.64 NKK796 pKa = 10.2 GWCTSKK802 pKa = 10.85 CDD804 pKa = 4.38 GKK806 pKa = 10.33 MKK808 pKa = 9.98 PAGYY812 pKa = 10.18 RR813 pKa = 11.84 NQLCVNGICKK823 pKa = 8.67 VQPVSSACCYY833 pKa = 9.33 WYY835 pKa = 9.18 VTIFTCGSSYY845 pKa = 11.1 KK846 pKa = 10.32 CGGVTSMIRR855 pKa = 11.84 TEE857 pKa = 3.87 TDD859 pKa = 3.23 GNHH862 pKa = 5.11 GWVKK866 pKa = 10.38 GVGRR870 pKa = 11.84 GEE872 pKa = 4.29 ALTTVAVSVRR882 pKa = 11.84 TNGGDD887 pKa = 3.44 LKK889 pKa = 11.11 EE890 pKa = 4.29 SDD892 pKa = 3.96 GVIKK896 pKa = 10.75 GVGTHH901 pKa = 6.36 KK902 pKa = 10.6 SGPITVRR909 pKa = 11.84 VKK911 pKa = 10.78 GVANLPQINFGGHH924 pKa = 6.86 KK925 pKa = 9.75 IYY927 pKa = 9.98 ATDD930 pKa = 3.25 TATYY934 pKa = 7.93 LTLASDD940 pKa = 3.73 INNPVVGTIGDD951 pKa = 3.91 VQASSAGAWTNPPEE965 pKa = 4.9 AITGEE970 pKa = 3.91 EE971 pKa = 4.01 HH972 pKa = 7.27 LEE974 pKa = 4.0 HH975 pKa = 6.84 FRR977 pKa = 11.84 TADD980 pKa = 3.66 VVDD983 pKa = 5.51 LSCSSWDD990 pKa = 3.44 MSILQSEE997 pKa = 4.24 PGINLLHH1004 pKa = 6.75 DD1005 pKa = 3.83 QFSDD1009 pKa = 3.37 TQLPVVFQGSAFKK1022 pKa = 10.47 EE1023 pKa = 4.28 VSNEE1027 pKa = 3.65 RR1028 pKa = 11.84 LIEE1031 pKa = 4.13 EE1032 pKa = 4.46 LTGSRR1037 pKa = 11.84 PLSVEE1042 pKa = 3.52 IAISGTFAVKK1052 pKa = 10.15 HH1053 pKa = 5.54 VIKK1056 pKa = 10.11 EE1057 pKa = 4.37 CKK1059 pKa = 9.5 PEE1061 pKa = 3.86 MEE1063 pKa = 4.2 FVEE1066 pKa = 4.43 MKK1068 pKa = 10.82 GCFSCYY1074 pKa = 10.01 NPGAILTVRR1083 pKa = 11.84 TRR1085 pKa = 11.84 SNNPYY1090 pKa = 9.94 SCVAALTTNSEE1101 pKa = 4.35 HH1102 pKa = 6.35 FNCPGSLEE1110 pKa = 4.15 FEE1112 pKa = 4.8 PGDD1115 pKa = 3.48 FVEE1118 pKa = 5.55 RR1119 pKa = 11.84 KK1120 pKa = 8.87 VSCISSEE1127 pKa = 4.12 EE1128 pKa = 4.05 EE1129 pKa = 3.42 NDD1131 pKa = 3.52 IVLTAQTRR1139 pKa = 11.84 TKK1141 pKa = 10.8 VSVEE1145 pKa = 3.85 VTGTLLADD1153 pKa = 3.92 RR1154 pKa = 11.84 LLAANEE1160 pKa = 4.16 LEE1162 pKa = 4.22 GAEE1165 pKa = 4.26 PDD1167 pKa = 3.59 EE1168 pKa = 4.93 SSSVLNPLGLSDD1180 pKa = 5.83 LNITLISIGSVTTFVVIFILLACFCGPFRR1209 pKa = 11.84 DD1210 pKa = 4.17 CLVFACPCLRR1220 pKa = 11.84 SLSRR1224 pKa = 11.84 KK1225 pKa = 9.99 LPDD1228 pKa = 3.58 KK1229 pKa = 11.24 LSGFKK1234 pKa = 10.79 DD1235 pKa = 3.44 KK1236 pKa = 10.56 IDD1238 pKa = 3.79 RR1239 pKa = 11.84 SAEE1242 pKa = 3.86 KK1243 pKa = 10.66 AKK1245 pKa = 10.97 VSGIKK1250 pKa = 10.24 AKK1252 pKa = 9.96 SEE1254 pKa = 4.21 KK1255 pKa = 10.15 EE1256 pKa = 3.36 WGEE1259 pKa = 3.79 AKK1261 pKa = 10.26 KK1262 pKa = 10.71 AQDD1265 pKa = 3.27 NHH1267 pKa = 6.37 DD1268 pKa = 3.49 KK1269 pKa = 10.19 VVRR1272 pKa = 11.84 MRR1274 pKa = 11.84 KK1275 pKa = 9.6 KK1276 pKa = 10.44 FGQAA1280 pKa = 2.79
Molecular weight: 141.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.619
IPC2_protein 5.766
IPC_protein 5.792
Toseland 5.931
ProMoST 6.135
Dawson 6.046
Bjellqvist 6.084
Wikipedia 6.033
Rodwell 6.033
Grimsley 5.97
Solomon 6.046
Lehninger 6.033
Nozaki 6.313
DTASelect 6.478
Thurlkill 6.465
EMBOSS 6.427
Sillero 6.415
Patrickios 3.757
IPC_peptide 6.059
IPC2_peptide 6.364
IPC2.peptide.svr19 6.381
Protein with the highest isoelectric point:
>tr|A0A1L3KPD6|A0A1L3KPD6_9VIRU Uncharacterized protein OS=Beihai shrimp virus 3 OX=1922669 PE=4 SV=1
MM1 pKa = 7.69 SSRR4 pKa = 11.84 ISIKK8 pKa = 10.25 YY9 pKa = 9.49 LPEE12 pKa = 3.9 VTFKK16 pKa = 10.97 AANGEE21 pKa = 4.23 ALFSPKK27 pKa = 9.9 FEE29 pKa = 4.23 RR30 pKa = 11.84 TGVTSRR36 pKa = 11.84 EE37 pKa = 4.25 SLSGAVNRR45 pKa = 11.84 ALNCMVRR52 pKa = 11.84 VKK54 pKa = 10.61 DD55 pKa = 3.94 FQVVGHH61 pKa = 6.66 AFITKK66 pKa = 10.12 LIAAGHH72 pKa = 6.62 DD73 pKa = 3.75 PYY75 pKa = 11.72 VDD77 pKa = 3.35 AVVEE81 pKa = 3.99 ALIQGDD87 pKa = 4.41 DD88 pKa = 4.27 DD89 pKa = 3.86 NTLTTKK95 pKa = 11.04 YY96 pKa = 8.9 MVAMDD101 pKa = 4.27 KK102 pKa = 10.99 VAILSGIPKK111 pKa = 8.41 EE112 pKa = 4.4 TGSVATEE119 pKa = 3.94 LKK121 pKa = 10.54 VAASQLNSVNDD132 pKa = 4.93 LISAMKK138 pKa = 9.9 QNSSAGMSSTHH149 pKa = 5.81 VVDD152 pKa = 4.95 VYY154 pKa = 11.48 TNVLNLSACCNGSTHH169 pKa = 7.16 SYY171 pKa = 10.91 LPDD174 pKa = 3.8 LATNSKK180 pKa = 7.43 GQKK183 pKa = 9.15 MIVTAMVSRR192 pKa = 11.84 PRR194 pKa = 11.84 LNQRR198 pKa = 11.84 DD199 pKa = 4.16 RR200 pKa = 11.84 DD201 pKa = 4.01 LLDD204 pKa = 3.8 AALNLIGLNSPDD216 pKa = 4.7 HH217 pKa = 5.88 IALYY221 pKa = 11.22 NEE223 pKa = 4.26 MQQMAGSGAAVVANNSKK240 pKa = 10.36 NICYY244 pKa = 9.46 PFYY247 pKa = 10.45 ISLVAAMMFRR257 pKa = 11.84 DD258 pKa = 3.96 NKK260 pKa = 10.2 TDD262 pKa = 3.26 TDD264 pKa = 3.93 VQSFLIPFVKK274 pKa = 9.51 MAKK277 pKa = 8.11 TKK279 pKa = 10.48 RR280 pKa = 11.84 GAATKK285 pKa = 10.21 SHH287 pKa = 7.06 NILQAYY293 pKa = 7.39 NFPGYY298 pKa = 9.74 IQGGKK303 pKa = 9.83 DD304 pKa = 2.77 IHH306 pKa = 7.15 LNPCYY311 pKa = 9.16 LTPYY315 pKa = 9.68 RR316 pKa = 11.84 MCRR319 pKa = 11.84 ALMAAAPMPLVTNARR334 pKa = 11.84 GIMMTPEE341 pKa = 4.33 SIAFVLRR348 pKa = 11.84 LYY350 pKa = 10.71 EE351 pKa = 4.75 IDD353 pKa = 3.83 VEE355 pKa = 4.71 LGGDD359 pKa = 3.66 RR360 pKa = 11.84 NINDD364 pKa = 3.4 ATSIFHH370 pKa = 6.57 HH371 pKa = 6.29 MLVGGIGSYY380 pKa = 9.29 ATTHH384 pKa = 6.04 NKK386 pKa = 8.28 TGNALNVTVKK396 pKa = 10.64 LSGQNYY402 pKa = 7.87 TPAPPMTQVMTVVAQPQPQRR422 pKa = 11.84 AAAPMAPTAPPTAAVAPPPAPAPATDD448 pKa = 3.29 RR449 pKa = 11.84 LGNIVPTAPAPVPGTVPAPPAIAPLPGAIATSATTTVTVPQQQQPQQQQAPAQAPATTAAATPTVAVPGATAGISTNTATPSNAGNNSNAA539 pKa = 3.65
Molecular weight: 56.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.924
IPC2_protein 8.068
IPC_protein 7.98
Toseland 8.009
ProMoST 8.463
Dawson 8.712
Bjellqvist 8.873
Wikipedia 8.726
Rodwell 8.77
Grimsley 7.98
Solomon 8.829
Lehninger 8.814
Nozaki 8.975
DTASelect 8.682
Thurlkill 8.741
EMBOSS 8.887
Sillero 9.004
Patrickios 4.304
IPC_peptide 8.829
IPC2_peptide 7.717
IPC2.peptide.svr19 7.83
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
5671
312
3540
1417.8
158.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.018 ± 1.056
2.451 ± 0.866
5.414 ± 0.47
6.648 ± 0.977
3.527 ± 0.242
5.678 ± 0.908
2.028 ± 0.075
4.761 ± 0.083
6.048 ± 0.362
9.646 ± 0.869
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.839 ± 0.305
4.109 ± 0.378
4.303 ± 0.783
2.998 ± 0.258
5.537 ± 0.716
8.852 ± 0.431
6.524 ± 0.393
7.371 ± 0.523
1.111 ± 0.279
3.139 ± 0.277
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here