Bombiscardovia coagulans
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1438 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A261ESU0|A0A261ESU0_9BIFI Uridylate kinase OS=Bombiscardovia coagulans OX=686666 GN=pyrH PE=3 SV=1
MM1 pKa = 7.9 DD2 pKa = 4.92 NEE4 pKa = 4.42 GKK6 pKa = 10.07 NLGKK10 pKa = 10.51 VFAIYY15 pKa = 10.39 NSEE18 pKa = 4.05 YY19 pKa = 9.82 PVFGIEE25 pKa = 4.65 FIRR28 pKa = 11.84 VEE30 pKa = 4.95 DD31 pKa = 3.6 EE32 pKa = 3.9 DD33 pKa = 3.74 QIYY36 pKa = 10.17 EE37 pKa = 4.21 VAQQSSEE44 pKa = 4.06 LFNDD48 pKa = 3.42 EE49 pKa = 4.52 EE50 pKa = 4.92 IDD52 pKa = 3.96 VIRR55 pKa = 11.84 EE56 pKa = 3.51 RR57 pKa = 11.84 LIAIRR62 pKa = 11.84 VEE64 pKa = 4.0 YY65 pKa = 10.7 LDD67 pKa = 5.65 DD68 pKa = 5.04 YY69 pKa = 11.64 EE70 pKa = 6.37 DD71 pKa = 4.95 LDD73 pKa = 4.45 SPDD76 pKa = 4.17 AIKK79 pKa = 10.4 AYY81 pKa = 10.02 SQYY84 pKa = 10.63 GWQTSEE90 pKa = 4.59 SEE92 pKa = 4.24 LVPDD96 pKa = 5.34 GSLSDD101 pKa = 4.11 GDD103 pKa = 4.72 SISNSDD109 pKa = 3.42 LSTYY113 pKa = 10.61 QEE115 pKa = 4.1 DD116 pKa = 3.96 QYY118 pKa = 11.94 DD119 pKa = 4.06 RR120 pKa = 11.84 CEE122 pKa = 3.65 TTYY125 pKa = 10.7 PDD127 pKa = 4.07 KK128 pKa = 11.15 YY129 pKa = 11.25 NPGAVEE135 pKa = 3.76 AA136 pKa = 4.85
Molecular weight: 15.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.749
IPC2_protein 3.681
IPC_protein 3.668
Toseland 3.465
ProMoST 3.821
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.567
Rodwell 3.503
Grimsley 3.376
Solomon 3.643
Lehninger 3.592
Nozaki 3.77
DTASelect 3.961
Thurlkill 3.516
EMBOSS 3.579
Sillero 3.783
Patrickios 0.693
IPC_peptide 3.643
IPC2_peptide 3.757
IPC2.peptide.svr19 3.719
Protein with the highest isoelectric point:
>tr|A0A261EQP2|A0A261EQP2_9BIFI Thioredoxin reductase OS=Bombiscardovia coagulans OX=686666 GN=BOCO_0989 PE=3 SV=1
MM1 pKa = 7.52 AVLTGALNMKK11 pKa = 9.16 RR12 pKa = 11.84 TFQPNNRR19 pKa = 11.84 RR20 pKa = 11.84 RR21 pKa = 11.84 HH22 pKa = 4.79 MKK24 pKa = 9.36 HH25 pKa = 5.81 GFRR28 pKa = 11.84 ARR30 pKa = 11.84 MRR32 pKa = 11.84 TRR34 pKa = 11.84 SGRR37 pKa = 11.84 ALINRR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 AKK46 pKa = 10.08 GRR48 pKa = 11.84 KK49 pKa = 8.87 SLSAA53 pKa = 3.86
Molecular weight: 6.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1438
0
1438
505083
29
3148
351.2
38.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.439 ± 0.056
0.95 ± 0.022
5.893 ± 0.054
5.466 ± 0.06
3.389 ± 0.038
7.477 ± 0.063
2.368 ± 0.033
5.717 ± 0.059
4.187 ± 0.056
9.329 ± 0.08
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.455 ± 0.029
3.551 ± 0.04
4.609 ± 0.041
4.797 ± 0.049
5.345 ± 0.054
7.314 ± 0.055
5.876 ± 0.05
7.67 ± 0.061
1.411 ± 0.028
2.757 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here