Cervus elaphus papillomavirus 2
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I9KHZ0|A0A1I9KHZ0_9PAPI Replication protein E1 OS=Cervus elaphus papillomavirus 2 OX=1747359 GN=E1 PE=3 SV=1
MM1 pKa = 7.73 RR2 pKa = 11.84 GDD4 pKa = 4.04 KK5 pKa = 9.39 PTISDD10 pKa = 3.28 IALDD14 pKa = 4.36 LEE16 pKa = 4.68 QIVCPVSLEE25 pKa = 4.31 CNEE28 pKa = 4.07 TMSPEE33 pKa = 3.94 EE34 pKa = 4.8 AEE36 pKa = 4.34 AALQFPYY43 pKa = 10.3 RR44 pKa = 11.84 VATSCSGCNKK54 pKa = 9.41 EE55 pKa = 3.98 LHH57 pKa = 6.45 LFVAATEE64 pKa = 3.97 EE65 pKa = 4.93 GIRR68 pKa = 11.84 SLEE71 pKa = 3.81 VSLIGDD77 pKa = 4.25 KK78 pKa = 11.13 LSLLCGRR85 pKa = 11.84 CARR88 pKa = 11.84 VIFSNGQRR96 pKa = 3.41
Molecular weight: 10.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.714
IPC2_protein 4.851
IPC_protein 4.673
Toseland 4.546
ProMoST 4.762
Dawson 4.596
Bjellqvist 4.749
Wikipedia 4.444
Rodwell 4.52
Grimsley 4.457
Solomon 4.596
Lehninger 4.546
Nozaki 4.711
DTASelect 4.8
Thurlkill 4.533
EMBOSS 4.457
Sillero 4.787
Patrickios 3.605
IPC_peptide 4.609
IPC2_peptide 4.774
IPC2.peptide.svr19 4.781
Protein with the highest isoelectric point:
>tr|A0A1I9KHZ8|A0A1I9KHZ8_9PAPI Regulatory protein E2 OS=Cervus elaphus papillomavirus 2 OX=1747359 GN=E2 PE=3 SV=1
MM1 pKa = 7.79 EE2 pKa = 4.97 NLQARR7 pKa = 11.84 FDD9 pKa = 4.01 VVQEE13 pKa = 3.98 KK14 pKa = 10.65 LMEE17 pKa = 4.76 IYY19 pKa = 10.46 EE20 pKa = 4.48 SDD22 pKa = 3.41 VQTIDD27 pKa = 3.15 KK28 pKa = 10.63 QIEE31 pKa = 3.74 HH32 pKa = 6.42 WQTVRR37 pKa = 11.84 LEE39 pKa = 4.02 QVLLFVARR47 pKa = 11.84 KK48 pKa = 9.85 KK49 pKa = 11.0 GITRR53 pKa = 11.84 MGLQVVPPTAVSEE66 pKa = 4.19 ARR68 pKa = 11.84 AKK70 pKa = 10.55 NAIEE74 pKa = 3.89 MVLVLEE80 pKa = 4.28 RR81 pKa = 11.84 LKK83 pKa = 11.19 DD84 pKa = 3.52 SAYY87 pKa = 9.45 GTEE90 pKa = 4.48 KK91 pKa = 8.78 WTLTDD96 pKa = 4.21 SSRR99 pKa = 11.84 EE100 pKa = 4.09 TYY102 pKa = 9.83 KK103 pKa = 10.85 AAPEE107 pKa = 3.96 DD108 pKa = 4.04 TFKK111 pKa = 10.97 KK112 pKa = 10.01 RR113 pKa = 11.84 PASVTVIYY121 pKa = 10.94 DD122 pKa = 3.51 GDD124 pKa = 4.1 AYY126 pKa = 11.64 NSMIYY131 pKa = 9.5 TMWRR135 pKa = 11.84 DD136 pKa = 2.91 IYY138 pKa = 11.51 YY139 pKa = 10.51 EE140 pKa = 5.5 DD141 pKa = 3.34 DD142 pKa = 3.98 TGWHH146 pKa = 6.18 KK147 pKa = 11.07 VPSQVDD153 pKa = 3.77 YY154 pKa = 11.51 YY155 pKa = 11.05 GIYY158 pKa = 10.09 YY159 pKa = 9.81 VDD161 pKa = 4.0 HH162 pKa = 6.82 EE163 pKa = 4.86 GEE165 pKa = 3.79 KK166 pKa = 10.2 KK167 pKa = 10.82 YY168 pKa = 11.19 YY169 pKa = 10.57 VLFEE173 pKa = 4.74 KK174 pKa = 10.92 DD175 pKa = 2.72 ADD177 pKa = 4.0 LYY179 pKa = 11.26 SRR181 pKa = 11.84 NGQWEE186 pKa = 4.25 VQFEE190 pKa = 4.17 NMQFSAPVTSSTTGSSRR207 pKa = 11.84 HH208 pKa = 5.32 GPEE211 pKa = 3.7 TASTRR216 pKa = 11.84 EE217 pKa = 4.05 DD218 pKa = 3.61 PQASSAGPPAARR230 pKa = 11.84 PRR232 pKa = 11.84 QPASPTRR239 pKa = 11.84 RR240 pKa = 11.84 RR241 pKa = 11.84 HH242 pKa = 5.13 RR243 pKa = 11.84 APLVSSLLSHH253 pKa = 6.4 RR254 pKa = 11.84 RR255 pKa = 11.84 SRR257 pKa = 11.84 SRR259 pKa = 11.84 SGSSRR264 pKa = 11.84 SSRR267 pKa = 11.84 SSRR270 pKa = 11.84 SSRR273 pKa = 11.84 SRR275 pKa = 11.84 SPAKK279 pKa = 9.81 RR280 pKa = 11.84 PRR282 pKa = 11.84 RR283 pKa = 11.84 RR284 pKa = 11.84 GRR286 pKa = 11.84 RR287 pKa = 11.84 GRR289 pKa = 11.84 RR290 pKa = 11.84 VARR293 pKa = 11.84 SGSRR297 pKa = 11.84 GSSSDD302 pKa = 3.09 SSRR305 pKa = 11.84 TEE307 pKa = 3.69 PGGQSPPSPEE317 pKa = 3.86 QVGEE321 pKa = 4.08 RR322 pKa = 11.84 SRR324 pKa = 11.84 LPPRR328 pKa = 11.84 EE329 pKa = 3.8 ASSRR333 pKa = 11.84 LARR336 pKa = 11.84 LISEE340 pKa = 4.38 ARR342 pKa = 11.84 DD343 pKa = 3.54 PPVLLVKK350 pKa = 10.59 GSPNVLRR357 pKa = 11.84 CWRR360 pKa = 11.84 RR361 pKa = 11.84 RR362 pKa = 11.84 CRR364 pKa = 11.84 LRR366 pKa = 11.84 YY367 pKa = 8.78 SAYY370 pKa = 8.34 FQSMTTTFTWISGHH384 pKa = 4.82 STDD387 pKa = 4.15 RR388 pKa = 11.84 LGGNRR393 pKa = 11.84 MLVGFADD400 pKa = 4.7 TIQRR404 pKa = 11.84 NEE406 pKa = 4.06 FLNKK410 pKa = 9.02 VKK412 pKa = 10.01 MPKK415 pKa = 9.85 RR416 pKa = 11.84 VTWAYY421 pKa = 10.37 GALDD425 pKa = 3.61 TFF427 pKa = 4.81
Molecular weight: 48.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.298
IPC2_protein 9.575
IPC_protein 10.262
Toseland 10.248
ProMoST 10.028
Dawson 10.452
Bjellqvist 10.175
Wikipedia 10.672
Rodwell 10.643
Grimsley 10.54
Solomon 10.511
Lehninger 10.467
Nozaki 10.233
DTASelect 10.175
Thurlkill 10.306
EMBOSS 10.672
Sillero 10.379
Patrickios 10.145
IPC_peptide 10.511
IPC2_peptide 9.019
IPC2.peptide.svr19 8.682
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2297
96
601
382.8
43.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.834 ± 0.637
2.264 ± 0.81
5.921 ± 0.338
6.182 ± 0.401
4.615 ± 0.482
6.487 ± 0.692
1.785 ± 0.093
4.441 ± 0.675
5.05 ± 0.612
8.489 ± 0.807
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.48 ± 0.227
4.179 ± 0.614
6.313 ± 0.847
4.136 ± 0.331
7.357 ± 1.216
8.098 ± 0.872
6.356 ± 0.556
6.051 ± 0.284
1.524 ± 0.311
3.439 ± 0.306
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here