Aspergillus fumigatus partitivirus 2
Average proteome isoelectric point is 8.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A344X3Q1|A0A344X3Q1_9VIRU Coat protein OS=Aspergillus fumigatus partitivirus 2 OX=2250452 PE=4 SV=1
MM1 pKa = 7.76 LLTEE5 pKa = 4.83 IPNVYY10 pKa = 9.55 QRR12 pKa = 11.84 AVALEE17 pKa = 3.5 RR18 pKa = 11.84 RR19 pKa = 11.84 IRR21 pKa = 11.84 LRR23 pKa = 11.84 EE24 pKa = 3.99 LYY26 pKa = 9.99 PEE28 pKa = 4.45 GVVPYY33 pKa = 9.87 LAKK36 pKa = 10.25 QEE38 pKa = 4.0 YY39 pKa = 9.73 DD40 pKa = 3.32 GAIAGPHH47 pKa = 6.09 GPHH50 pKa = 6.66 GSVAPSVTTTFQAKK64 pKa = 10.11 LPLDD68 pKa = 3.5 QYY70 pKa = 11.09 PDD72 pKa = 2.91 RR73 pKa = 11.84 KK74 pKa = 9.18 YY75 pKa = 9.2 TLEE78 pKa = 4.19 CKK80 pKa = 9.11 EE81 pKa = 4.02 TMIYY85 pKa = 9.18 LGKK88 pKa = 9.38 MDD90 pKa = 5.39 GYY92 pKa = 9.36 PFSSSIPKK100 pKa = 9.62 FDD102 pKa = 2.79 PWFRR106 pKa = 11.84 KK107 pKa = 8.56 VLKK110 pKa = 10.45 DD111 pKa = 3.41 KK112 pKa = 11.22 DD113 pKa = 3.5 PAIVKK118 pKa = 10.11 DD119 pKa = 4.17 LEE121 pKa = 4.31 DD122 pKa = 3.03 TFMRR126 pKa = 11.84 DD127 pKa = 2.47 PCTPDD132 pKa = 3.26 RR133 pKa = 11.84 VMKK136 pKa = 10.68 HH137 pKa = 5.03 MSLFDD142 pKa = 3.87 RR143 pKa = 11.84 TWKK146 pKa = 10.46 RR147 pKa = 11.84 MPTGRR152 pKa = 11.84 LMSRR156 pKa = 11.84 AKK158 pKa = 10.72 DD159 pKa = 3.15 IVAKK163 pKa = 9.7 MFEE166 pKa = 4.78 PIGKK170 pKa = 9.2 VEE172 pKa = 4.81 PIDD175 pKa = 3.94 FNFAGWHH182 pKa = 6.16 KK183 pKa = 10.4 ILPNLDD189 pKa = 3.54 MTSSPGLPLRR199 pKa = 11.84 RR200 pKa = 11.84 EE201 pKa = 3.99 YY202 pKa = 9.28 QTQGEE207 pKa = 4.78 CLGHH211 pKa = 7.8 IYY213 pKa = 10.92 DD214 pKa = 3.46 KK215 pKa = 10.74 TKK217 pKa = 10.79 RR218 pKa = 11.84 LNHH221 pKa = 6.11 FAKK224 pKa = 9.98 FLHH227 pKa = 6.44 PAAVRR232 pKa = 11.84 APPCMIGVRR241 pKa = 11.84 PGLIRR246 pKa = 11.84 RR247 pKa = 11.84 DD248 pKa = 3.54 EE249 pKa = 4.2 YY250 pKa = 11.46 NEE252 pKa = 3.93 KK253 pKa = 10.5 VKK255 pKa = 10.98 ARR257 pKa = 11.84 GVWAYY262 pKa = 8.57 PAEE265 pKa = 4.33 VKK267 pKa = 10.51 VMEE270 pKa = 4.24 MRR272 pKa = 11.84 FVQPLIEE279 pKa = 4.34 RR280 pKa = 11.84 MSLNFMKK287 pKa = 10.19 IPYY290 pKa = 9.09 PVGRR294 pKa = 11.84 NMTKK298 pKa = 10.48 ALPMLIDD305 pKa = 4.84 HH306 pKa = 6.8 MLHH309 pKa = 6.61 DD310 pKa = 4.34 KK311 pKa = 11.08 KK312 pKa = 10.95 KK313 pKa = 10.71 GFVTDD318 pKa = 3.47 VSNLDD323 pKa = 3.44 ASVGPDD329 pKa = 3.44 YY330 pKa = 11.06 IDD332 pKa = 3.08 WAFSLMKK339 pKa = 9.47 TWFNFGITQSSEE351 pKa = 3.55 TRR353 pKa = 11.84 NANVFDD359 pKa = 3.88 FLHH362 pKa = 6.77 YY363 pKa = 9.89 YY364 pKa = 10.23 FKK366 pKa = 10.31 RR367 pKa = 11.84 TPILLPSGQLVKK379 pKa = 10.69 KK380 pKa = 10.69 AGGVPSGSGFTQLVDD395 pKa = 3.52 TLVTILATVYY405 pKa = 9.84 TLLRR409 pKa = 11.84 MGWDD413 pKa = 3.28 EE414 pKa = 4.02 DD415 pKa = 4.11 QIIKK419 pKa = 10.81 GYY421 pKa = 7.41 MQVVGDD427 pKa = 4.4 DD428 pKa = 3.71 MAVSVPRR435 pKa = 11.84 DD436 pKa = 3.38 FDD438 pKa = 3.81 PEE440 pKa = 4.04 EE441 pKa = 3.86 FVYY444 pKa = 10.86 LMGSLGFTINLKK456 pKa = 10.55 KK457 pKa = 11.25 VMFSDD462 pKa = 4.23 KK463 pKa = 10.96 GIEE466 pKa = 4.11 LKK468 pKa = 10.83 FLGYY472 pKa = 9.85 SKK474 pKa = 11.18 YY475 pKa = 10.23 GGSVYY480 pKa = 10.49 RR481 pKa = 11.84 PLDD484 pKa = 3.69 EE485 pKa = 5.16 LLQTAFFPEE494 pKa = 4.37 KK495 pKa = 10.36 FVGNPEE501 pKa = 3.98 RR502 pKa = 11.84 SRR504 pKa = 11.84 MRR506 pKa = 11.84 IAGQTLASGMTNAFFSKK523 pKa = 9.6 VNYY526 pKa = 9.05 WMEE529 pKa = 4.19 EE530 pKa = 4.11 LVSLATEE537 pKa = 4.63 LDD539 pKa = 3.55 PDD541 pKa = 3.94 EE542 pKa = 5.23 VFIPQRR548 pKa = 11.84 RR549 pKa = 11.84 WLRR552 pKa = 11.84 IVLGLDD558 pKa = 3.22 EE559 pKa = 4.94 MPSTTLAFDD568 pKa = 3.74 MFPLVV573 pKa = 3.42
Molecular weight: 65.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.105
IPC2_protein 8.185
IPC_protein 8.083
Toseland 8.609
ProMoST 8.58
Dawson 8.99
Bjellqvist 8.873
Wikipedia 9.136
Rodwell 9.121
Grimsley 8.96
Solomon 9.063
Lehninger 9.033
Nozaki 8.916
DTASelect 8.785
Thurlkill 8.873
EMBOSS 9.121
Sillero 9.048
Patrickios 4.584
IPC_peptide 9.063
IPC2_peptide 7.512
IPC2.peptide.svr19 7.599
Protein with the highest isoelectric point:
>tr|A0A344X3Q1|A0A344X3Q1_9VIRU Coat protein OS=Aspergillus fumigatus partitivirus 2 OX=2250452 PE=4 SV=1
MM1 pKa = 7.41 PRR3 pKa = 11.84 KK4 pKa = 8.91 DD5 pKa = 3.39 TAEE8 pKa = 4.31 KK9 pKa = 11.11 DD10 pKa = 3.57 PVTKK14 pKa = 10.52 DD15 pKa = 3.12 DD16 pKa = 3.87 TKK18 pKa = 10.59 GQKK21 pKa = 9.57 DD22 pKa = 3.38 AAADD26 pKa = 3.68 STDD29 pKa = 3.17 KK30 pKa = 11.59 GKK32 pKa = 11.17 GNKK35 pKa = 9.34 PKK37 pKa = 8.99 TQKK40 pKa = 10.56 QKK42 pKa = 9.72 TEE44 pKa = 4.01 SSASKK49 pKa = 8.23 YY50 pKa = 9.09 TPGFAEE56 pKa = 4.84 GNSVLQGYY64 pKa = 8.47 NLSVSGRR71 pKa = 11.84 MEE73 pKa = 5.38 RR74 pKa = 11.84 ILSPFKK80 pKa = 10.52 GDD82 pKa = 3.25 KK83 pKa = 10.25 FFHH86 pKa = 6.32 FVSDD90 pKa = 3.07 AWSEE94 pKa = 4.09 FTRR97 pKa = 11.84 LKK99 pKa = 10.27 PHH101 pKa = 4.62 ITEE104 pKa = 3.99 RR105 pKa = 11.84 FSYY108 pKa = 11.28 AEE110 pKa = 4.02 FRR112 pKa = 11.84 HH113 pKa = 5.59 MSALQLYY120 pKa = 9.33 NRR122 pKa = 11.84 LEE124 pKa = 4.02 AVKK127 pKa = 10.21 FDD129 pKa = 3.95 ALGVKK134 pKa = 9.86 QPAPTRR140 pKa = 11.84 IPLPRR145 pKa = 11.84 DD146 pKa = 3.13 TRR148 pKa = 11.84 VFQPIWSVLANIGVVEE164 pKa = 4.7 DD165 pKa = 3.84 PDD167 pKa = 3.64 LRR169 pKa = 11.84 AIYY172 pKa = 9.86 IPDD175 pKa = 4.78 GIVPKK180 pKa = 10.73 SKK182 pKa = 10.58 DD183 pKa = 3.47 LSHH186 pKa = 7.52 PDD188 pKa = 4.12 DD189 pKa = 4.08 IEE191 pKa = 4.52 NLLSCTLYY199 pKa = 10.75 DD200 pKa = 4.19 WEE202 pKa = 4.73 SSWKK206 pKa = 9.48 EE207 pKa = 3.57 VVKK210 pKa = 10.85 ARR212 pKa = 11.84 EE213 pKa = 3.82 ARR215 pKa = 11.84 KK216 pKa = 9.51 QYY218 pKa = 7.72 QQRR221 pKa = 11.84 TGLDD225 pKa = 2.94 ATTDD229 pKa = 3.65 SNEE232 pKa = 4.12 TTVQLSKK239 pKa = 11.41 EE240 pKa = 3.94 EE241 pKa = 4.73 LIAKK245 pKa = 9.25 IQFFRR250 pKa = 11.84 KK251 pKa = 9.2 EE252 pKa = 3.37 ITRR255 pKa = 11.84 AEE257 pKa = 4.09 EE258 pKa = 4.13 RR259 pKa = 11.84 EE260 pKa = 3.91 KK261 pKa = 11.06 SPEE264 pKa = 3.81 YY265 pKa = 10.55 EE266 pKa = 3.92 LVKK269 pKa = 10.81 GVLRR273 pKa = 11.84 KK274 pKa = 9.7 IKK276 pKa = 10.51 AVTAPSSPSSSRR288 pKa = 11.84 SGKK291 pKa = 10.43 DD292 pKa = 2.77 NGTEE296 pKa = 3.62 ATSPKK301 pKa = 10.02 KK302 pKa = 10.41 EE303 pKa = 4.16 YY304 pKa = 10.12 EE305 pKa = 3.98 QEE307 pKa = 4.43 TYY309 pKa = 7.56 WTVSGAKK316 pKa = 9.14 NQLEE320 pKa = 4.81 AYY322 pKa = 7.1 MQQAKK327 pKa = 10.0 KK328 pKa = 10.59 LKK330 pKa = 9.88 SQMIAPKK337 pKa = 10.18 FDD339 pKa = 3.38 VTHH342 pKa = 6.89 KK343 pKa = 10.03 IEE345 pKa = 4.23 SYY347 pKa = 10.17 RR348 pKa = 11.84 ISDD351 pKa = 3.69 GTITTSPGSYY361 pKa = 9.68 GEE363 pKa = 3.96 WMRR366 pKa = 11.84 WDD368 pKa = 3.35 PQLYY372 pKa = 8.74 IDD374 pKa = 4.34 YY375 pKa = 9.47 EE376 pKa = 4.39 NFVTEE381 pKa = 4.29 VTPMALFSLSMPAEE395 pKa = 4.33 SKK397 pKa = 9.45 GTYY400 pKa = 9.76 AWILPVEE407 pKa = 4.22 KK408 pKa = 10.62 RR409 pKa = 11.84 EE410 pKa = 4.45 DD411 pKa = 3.89 DD412 pKa = 4.07 DD413 pKa = 5.53 SSVSARR419 pKa = 11.84 MPRR422 pKa = 11.84 ASIPTATWVLALLLQSSTLPLHH444 pKa = 7.0 RR445 pKa = 11.84 RR446 pKa = 11.84 STWYY450 pKa = 9.69 TEE452 pKa = 3.67 TDD454 pKa = 2.88 RR455 pKa = 11.84 LQNVLGVRR463 pKa = 11.84 RR464 pKa = 11.84 RR465 pKa = 11.84 YY466 pKa = 9.52 IKK468 pKa = 10.63 AAIKK472 pKa = 10.62 DD473 pKa = 3.73 PTAVEE478 pKa = 4.13 QYY480 pKa = 9.63 GTII483 pKa = 3.71
Molecular weight: 54.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.257
IPC2_protein 8.273
IPC_protein 8.185
Toseland 8.916
ProMoST 8.697
Dawson 9.209
Bjellqvist 8.902
Wikipedia 9.37
Rodwell 9.428
Grimsley 9.268
Solomon 9.268
Lehninger 9.224
Nozaki 8.946
DTASelect 8.887
Thurlkill 9.033
EMBOSS 9.341
Sillero 9.136
Patrickios 4.774
IPC_peptide 9.268
IPC2_peptide 7.395
IPC2.peptide.svr19 7.558
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1056
483
573
528.0
60.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.345 ± 0.767
0.473 ± 0.184
6.345 ± 0.092
6.25 ± 0.832
4.64 ± 1.061
5.682 ± 0.779
1.515 ± 0.189
4.735 ± 0.019
7.67 ± 0.709
8.428 ± 0.96
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.598 ± 1.2
2.462 ± 0.128
6.723 ± 0.497
3.409 ± 0.649
6.155 ± 0.039
6.818 ± 1.584
6.534 ± 0.922
6.439 ± 0.587
1.799 ± 0.187
3.977 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here