Odonata-associated circular virus-2

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 8.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UH49|A0A0B4UH49_9VIRU Replication-associated protein OS=Odonata-associated circular virus-2 OX=1592120 PE=3 SV=1
MM1 pKa = 7.69PFVINARR8 pKa = 11.84KK9 pKa = 8.93FLLTYY14 pKa = 7.3PQCQASKK21 pKa = 10.64EE22 pKa = 4.11EE23 pKa = 4.06VLARR27 pKa = 11.84LQSLGEE33 pKa = 4.14IKK35 pKa = 10.01WYY37 pKa = 8.65TVAIEE42 pKa = 3.95LHH44 pKa = 6.68EE45 pKa = 5.4DD46 pKa = 4.18GSPHH50 pKa = 6.3LHH52 pKa = 6.16SACHH56 pKa = 4.96YY57 pKa = 9.2SKK59 pKa = 10.84KK60 pKa = 10.04IHH62 pKa = 5.24TTNCRR67 pKa = 11.84FFDD70 pKa = 5.3FGATHH75 pKa = 7.13CNIQTLKK82 pKa = 8.65TQQDD86 pKa = 3.69FEE88 pKa = 4.46RR89 pKa = 11.84AEE91 pKa = 4.4SYY93 pKa = 10.23CRR95 pKa = 11.84KK96 pKa = 9.85HH97 pKa = 7.13GNFISNYY104 pKa = 6.04DD105 pKa = 3.44TKK107 pKa = 10.91VPKK110 pKa = 10.18RR111 pKa = 11.84VLLAKK116 pKa = 10.44KK117 pKa = 10.33LIDD120 pKa = 4.3HH121 pKa = 6.19GQIDD125 pKa = 4.21YY126 pKa = 11.43DD127 pKa = 4.44FIQEE131 pKa = 3.89NPEE134 pKa = 4.01IIFFNYY140 pKa = 10.18SSLNSYY146 pKa = 10.67LSLVPKK152 pKa = 10.56KK153 pKa = 10.0KK154 pKa = 10.69VKK156 pKa = 10.57LNYY159 pKa = 7.91PTMKK163 pKa = 9.74QRR165 pKa = 11.84HH166 pKa = 5.16IWLHH170 pKa = 5.6GPSNSGKK177 pKa = 6.14TTWLRR182 pKa = 11.84DD183 pKa = 3.34YY184 pKa = 10.62LTDD187 pKa = 4.05RR188 pKa = 11.84NHH190 pKa = 7.26SEE192 pKa = 3.89IPTNNDD198 pKa = 2.67WCCNEE203 pKa = 3.83ATNVLWIDD211 pKa = 3.87EE212 pKa = 4.45YY213 pKa = 11.27KK214 pKa = 10.97GCLTIQQLNKK224 pKa = 10.49LCDD227 pKa = 3.51GDD229 pKa = 3.88TQLNRR234 pKa = 11.84KK235 pKa = 9.02GGSTRR240 pKa = 11.84ILFPTIVILSNFNISAVYY258 pKa = 10.74NNASDD263 pKa = 4.25DD264 pKa = 4.06MKK266 pKa = 10.6ATLYY270 pKa = 10.94NRR272 pKa = 11.84FIEE275 pKa = 4.36YY276 pKa = 10.08DD277 pKa = 3.58SSVSFPPVAA286 pKa = 4.41

Molecular weight: 33.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UH49|A0A0B4UH49_9VIRU Replication-associated protein OS=Odonata-associated circular virus-2 OX=1592120 PE=3 SV=1
MM1 pKa = 7.88CYY3 pKa = 10.4GRR5 pKa = 11.84SKK7 pKa = 10.28FKK9 pKa = 10.1RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84FIRR15 pKa = 11.84RR16 pKa = 11.84YY17 pKa = 9.27KK18 pKa = 9.16KK19 pKa = 8.72TGTRR23 pKa = 11.84FMARR27 pKa = 11.84AVKK30 pKa = 9.15RR31 pKa = 11.84TMYY34 pKa = 10.04RR35 pKa = 11.84QSEE38 pKa = 4.48TKK40 pKa = 10.36FFIVNVNSTITNAANYY56 pKa = 8.7EE57 pKa = 4.31SGWANGINQGTGNEE71 pKa = 3.93QRR73 pKa = 11.84IGNQIAVKK81 pKa = 10.56NIGFNFTFNFNTGNSGASGSFVRR104 pKa = 11.84VLVVWPRR111 pKa = 11.84KK112 pKa = 9.28GVSTTSLIGYY122 pKa = 7.58FTGTNPGIAARR133 pKa = 11.84PDD135 pKa = 3.61PKK137 pKa = 11.27LMLTLYY143 pKa = 9.99DD144 pKa = 3.65QKK146 pKa = 11.57FMLAANTAGNGLAFKK161 pKa = 9.42TCRR164 pKa = 11.84FTKK167 pKa = 8.11YY168 pKa = 10.81CKK170 pKa = 8.45MAKK173 pKa = 8.99WNYY176 pKa = 9.72DD177 pKa = 3.26SNGICDD183 pKa = 4.25SQPVVYY189 pKa = 9.79FATDD193 pKa = 3.31ASAVTTANISIRR205 pKa = 11.84GYY207 pKa = 9.73ATISYY212 pKa = 10.55KK213 pKa = 10.75DD214 pKa = 3.27VV215 pKa = 3.14

Molecular weight: 24.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

501

215

286

250.5

28.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.387 ± 1.52

2.595 ± 0.456

4.391 ± 0.993

3.194 ± 1.116

5.788 ± 0.737

5.988 ± 1.768

2.196 ± 1.362

6.587 ± 0.335

7.186 ± 0.13

6.587 ± 2.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.796 ± 0.617

8.184 ± 0.406

3.393 ± 0.662

3.593 ± 0.498

5.589 ± 1.15

6.986 ± 0.294

7.984 ± 1.107

4.99 ± 0.655

1.597 ± 0.125

4.99 ± 0.078
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski