Pseudonocardia sp.

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Pseudonocardia; unclassified Pseudonocardia

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 635 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A519DPX7|A0A519DPX7_9PSEU Mycofactocin system transcriptional regulator OS=Pseudonocardia sp. OX=60912 GN=mftR PE=4 SV=1
MM1 pKa = 7.41YY2 pKa = 9.84IAPLAQYY9 pKa = 10.55DD10 pKa = 3.76FLYY13 pKa = 10.71TEE15 pKa = 4.29VLGTDD20 pKa = 2.84ILEE23 pKa = 4.34TVTNGEE29 pKa = 4.0IKK31 pKa = 10.62AADD34 pKa = 3.5AAAVLTTAADD44 pKa = 3.71FAIEE48 pKa = 4.05MLHH51 pKa = 6.51PLNTVGDD58 pKa = 3.94RR59 pKa = 11.84AGSVLSDD66 pKa = 3.55GAVTTPVGYY75 pKa = 9.67KK76 pKa = 8.37QAYY79 pKa = 9.33RR80 pKa = 11.84SFAEE84 pKa = 4.29AGWVGFGLDD93 pKa = 3.66PAIDD97 pKa = 3.84GGGAPFLL104 pKa = 4.37

Molecular weight: 10.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A519DQ00|A0A519DQ00_9PSEU Uncharacterized protein OS=Pseudonocardia sp. OX=60912 GN=EOP16_03495 PE=4 SV=1
KKK2 pKa = 9.37TDDD5 pKa = 3.42DD6 pKa = 3.55KKK8 pKa = 11.14FKKK11 pKa = 10.02LPRR14 pKa = 11.84RR15 pKa = 11.84WVVEEE20 pKa = 3.93TFAWICKKK28 pKa = 9.07HH29 pKa = 5.22RR30 pKa = 11.84CVRR33 pKa = 11.84DDD35 pKa = 3.32YY36 pKa = 7.95EE37 pKa = 4.38RR38 pKa = 11.84PDDD41 pKa = 2.97HH42 pKa = 6.63HH43 pKa = 6.6EE44 pKa = 3.9MVYYY48 pKa = 9.83AMIATMSRR56 pKa = 11.84RR57 pKa = 11.84LARR60 pKa = 11.84TA

Molecular weight: 7.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

635

0

635

174812

27

1193

275.3

29.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.066 ± 0.128

0.922 ± 0.034

6.171 ± 0.091

5.492 ± 0.077

3.129 ± 0.062

8.694 ± 0.082

2.44 ± 0.055

4.707 ± 0.067

2.154 ± 0.055

9.587 ± 0.128

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.102 ± 0.039

2.489 ± 0.05

5.316 ± 0.072

2.996 ± 0.049

7.513 ± 0.113

6.004 ± 0.068

6.443 ± 0.074

8.108 ± 0.092

1.537 ± 0.038

2.13 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski