Salarchaeum sp. JOR-1
Average proteome isoelectric point is 4.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2527 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A518S9F0|A0A518S9F0_9EURY Plasmid stabilization protein OS=Salarchaeum sp. JOR-1 OX=2599399 GN=FQU85_03685 PE=4 SV=1
MM1 pKa = 7.72 NDD3 pKa = 3.55 DD4 pKa = 3.55 TNDD7 pKa = 3.46 RR8 pKa = 11.84 QLYY11 pKa = 7.99 NLSRR15 pKa = 11.84 RR16 pKa = 11.84 KK17 pKa = 10.02 VLGGLGAVGLASAGAGLGTSAYY39 pKa = 8.79 FTDD42 pKa = 3.96 TEE44 pKa = 4.72 SFTGNTLAAGEE55 pKa = 4.67 LDD57 pKa = 4.13 LKK59 pKa = 11.04 LDD61 pKa = 3.8 YY62 pKa = 10.94 KK63 pKa = 10.22 STYY66 pKa = 8.55 TGGPGRR72 pKa = 11.84 LEE74 pKa = 5.57 DD75 pKa = 3.98 IQAMGYY81 pKa = 9.76 PDD83 pKa = 5.61 AEE85 pKa = 4.32 DD86 pKa = 4.69 LGDD89 pKa = 3.77 GRR91 pKa = 11.84 YY92 pKa = 9.82 LLAQTPRR99 pKa = 11.84 PGDD102 pKa = 3.37 GMGWEE107 pKa = 4.77 DD108 pKa = 4.34 YY109 pKa = 11.08 LPEE112 pKa = 5.14 FDD114 pKa = 5.22 FCAPEE119 pKa = 3.86 NDD121 pKa = 3.47 QYY123 pKa = 11.84 LVNGDD128 pKa = 4.18 GISIFHH134 pKa = 6.97 LEE136 pKa = 4.11 DD137 pKa = 3.43 VKK139 pKa = 11.26 PGDD142 pKa = 3.82 SGEE145 pKa = 4.24 MTVSIHH151 pKa = 6.67 ICDD154 pKa = 3.52 NPAWLEE160 pKa = 3.9 MGGEE164 pKa = 4.02 LSEE167 pKa = 4.6 NADD170 pKa = 3.38 NTMSEE175 pKa = 4.54 PEE177 pKa = 4.28 SEE179 pKa = 4.28 VDD181 pKa = 3.47 STPDD185 pKa = 3.15 AGEE188 pKa = 3.89 LADD191 pKa = 5.0 AIEE194 pKa = 4.15 VTVWYY199 pKa = 10.19 DD200 pKa = 3.41 EE201 pKa = 5.36 DD202 pKa = 4.4 CDD204 pKa = 4.11 NVYY207 pKa = 10.8 EE208 pKa = 4.09 PTGTGEE214 pKa = 4.02 QQEE217 pKa = 4.62 LEE219 pKa = 4.31 VALVSDD225 pKa = 4.32 VSGSMSQEE233 pKa = 3.55 IGDD236 pKa = 4.38 LKK238 pKa = 10.83 SAAKK242 pKa = 10.58 GFVDD246 pKa = 3.95 NLSSPDD252 pKa = 3.19 EE253 pKa = 4.02 AAAISFSDD261 pKa = 4.64 GSSLDD266 pKa = 3.51 QQLTTNYY273 pKa = 7.86 QTVKK277 pKa = 10.79 DD278 pKa = 5.02 AIDD281 pKa = 3.49 QYY283 pKa = 10.94 TSGGGTDD290 pKa = 3.48 MDD292 pKa = 3.91 QGISTAEE299 pKa = 3.89 TEE301 pKa = 4.91 LISGANATSGASKK314 pKa = 11.08 VMIVLTDD321 pKa = 3.95 GQPSSQQAATTAAMNAKK338 pKa = 9.38 QAGIRR343 pKa = 11.84 IFTIALGSGAATSYY357 pKa = 11.09 LEE359 pKa = 4.22 NNIASSPGDD368 pKa = 3.45 AYY370 pKa = 11.07 VAPDD374 pKa = 4.33 PSDD377 pKa = 4.09 LDD379 pKa = 3.71 TVYY382 pKa = 11.06 SEE384 pKa = 3.93 IAQVVLAGEE393 pKa = 3.96 QLIAQGSLADD403 pKa = 4.18 VMALLAGGVEE413 pKa = 4.22 LDD415 pKa = 3.55 GDD417 pKa = 3.75 RR418 pKa = 11.84 TEE420 pKa = 5.17 EE421 pKa = 4.09 GRR423 pKa = 11.84 QPYY426 pKa = 9.28 PGNTTQCIGFEE437 pKa = 4.26 WVLPTDD443 pKa = 3.73 VEE445 pKa = 4.65 NEE447 pKa = 4.1 VQTDD451 pKa = 3.65 SVAFNVDD458 pKa = 3.85 FTATQYY464 pKa = 10.93 RR465 pKa = 11.84 HH466 pKa = 6.06 NPQTTTTAPQQ476 pKa = 3.04
Molecular weight: 50.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.605
IPC_protein 3.643
Toseland 3.414
ProMoST 3.795
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.554
Rodwell 3.465
Grimsley 3.325
Solomon 3.63
Lehninger 3.579
Nozaki 3.732
DTASelect 3.973
Thurlkill 3.465
EMBOSS 3.567
Sillero 3.757
Patrickios 1.316
IPC_peptide 3.617
IPC2_peptide 3.732
IPC2.peptide.svr19 3.701
Protein with the highest isoelectric point:
>tr|A0A518S9T5|A0A518S9T5_9EURY Polymer-forming cytoskeletal protein OS=Salarchaeum sp. JOR-1 OX=2599399 GN=FQU85_04265 PE=4 SV=1
MM1 pKa = 7.42 TLLPSRR7 pKa = 11.84 STLRR11 pKa = 11.84 AAAFVVLGTAGFAVSFTMTGQWIRR35 pKa = 11.84 WHH37 pKa = 5.75 MMGSRR42 pKa = 11.84 HH43 pKa = 6.42 SFSLQNGVLAALFTGLFALLALVGGIAIARR73 pKa = 11.84 RR74 pKa = 11.84 RR75 pKa = 3.59
Molecular weight: 8.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.462
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.29
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.281
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.047
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.161
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2527
0
2527
744264
37
3266
294.5
31.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.476 ± 0.086
0.661 ± 0.015
8.6 ± 0.078
8.101 ± 0.089
3.393 ± 0.035
8.266 ± 0.045
2.083 ± 0.026
3.585 ± 0.04
1.921 ± 0.03
9.056 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.664 ± 0.019
2.457 ± 0.032
4.552 ± 0.032
2.461 ± 0.032
6.519 ± 0.05
5.367 ± 0.033
6.441 ± 0.06
9.42 ± 0.057
1.116 ± 0.018
2.862 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here