Klebsiella phage NL_ZS_2
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7G9UTH3|A0A7G9UTH3_9CAUD Uncharacterized protein OS=Klebsiella phage NL_ZS_2 OX=2769331 PE=4 SV=1
MM1 pKa = 7.37 EE2 pKa = 5.49 RR3 pKa = 11.84 NANAYY8 pKa = 10.1 YY9 pKa = 10.68 DD10 pKa = 4.19 LLAATVEE17 pKa = 4.25 LFNEE21 pKa = 5.42 RR22 pKa = 11.84 IQQDD26 pKa = 4.36 EE27 pKa = 4.43 LTEE30 pKa = 4.32 DD31 pKa = 4.55 ADD33 pKa = 3.65 WSDD36 pKa = 3.57 ALHH39 pKa = 6.1 EE40 pKa = 4.49 VVDD43 pKa = 4.59 GQVPHH48 pKa = 6.66 YY49 pKa = 9.14 YY50 pKa = 10.31 SEE52 pKa = 4.24 IFTVMAADD60 pKa = 5.45 GIDD63 pKa = 3.77 HH64 pKa = 6.79 EE65 pKa = 5.26 FDD67 pKa = 4.04 DD68 pKa = 5.49 SGLIPDD74 pKa = 4.27 TKK76 pKa = 10.52 DD77 pKa = 3.0 VSRR80 pKa = 11.84 ICQARR85 pKa = 11.84 IYY87 pKa = 9.16 EE88 pKa = 4.1 ALYY91 pKa = 10.87 NDD93 pKa = 3.61 VSNDD97 pKa = 3.09 SGIIWWEE104 pKa = 3.81 EE105 pKa = 3.75 PEE107 pKa = 5.89 DD108 pKa = 4.06 EE109 pKa = 6.18 DD110 pKa = 5.71 EE111 pKa = 4.61 EE112 pKa = 5.45 CSDD115 pKa = 4.78 DD116 pKa = 3.91
Molecular weight: 13.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.772
IPC2_protein 3.592
IPC_protein 3.592
Toseland 3.376
ProMoST 3.719
Dawson 3.579
Bjellqvist 3.77
Wikipedia 3.516
Rodwell 3.414
Grimsley 3.287
Solomon 3.567
Lehninger 3.528
Nozaki 3.706
DTASelect 3.91
Thurlkill 3.439
EMBOSS 3.528
Sillero 3.706
Patrickios 0.655
IPC_peptide 3.567
IPC2_peptide 3.681
IPC2.peptide.svr19 3.702
Protein with the highest isoelectric point:
>tr|A0A7G9UTD2|A0A7G9UTD2_9CAUD Nucleotide kinase OS=Klebsiella phage NL_ZS_2 OX=2769331 PE=4 SV=1
MM1 pKa = 7.88 RR2 pKa = 11.84 LHH4 pKa = 6.7 FNKK7 pKa = 10.46 SNGIFSVRR15 pKa = 11.84 RR16 pKa = 11.84 EE17 pKa = 4.04 DD18 pKa = 3.55 RR19 pKa = 11.84 STVAASEE26 pKa = 4.13 RR27 pKa = 11.84 HH28 pKa = 4.99 GKK30 pKa = 8.95 IPRR33 pKa = 11.84 IGDD36 pKa = 3.66 TFEE39 pKa = 4.6 LAPSVHH45 pKa = 6.56 ILVTRR50 pKa = 11.84 GLYY53 pKa = 9.93 EE54 pKa = 4.12 LAQTKK59 pKa = 9.86 SRR61 pKa = 11.84 PFVPVVVTKK70 pKa = 9.98 WPRR73 pKa = 11.84 LRR75 pKa = 11.84 LFWEE79 pKa = 4.85 RR80 pKa = 11.84 IKK82 pKa = 11.06 EE83 pKa = 4.25 VVNDD87 pKa = 3.83
Molecular weight: 10.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 9.663
IPC_protein 10.687
Toseland 11.038
ProMoST 11.023
Dawson 11.067
Bjellqvist 10.862
Wikipedia 11.359
Rodwell 11.169
Grimsley 11.096
Solomon 11.33
Lehninger 11.286
Nozaki 11.008
DTASelect 10.862
Thurlkill 11.023
EMBOSS 11.462
Sillero 11.023
Patrickios 10.935
IPC_peptide 11.345
IPC2_peptide 9.882
IPC2.peptide.svr19 8.922
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
49
0
49
12401
36
1321
253.1
28.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.894 ± 0.531
0.911 ± 0.16
6.411 ± 0.201
6.943 ± 0.533
3.701 ± 0.17
7.975 ± 0.384
1.951 ± 0.198
4.83 ± 0.135
6.709 ± 0.376
8.193 ± 0.339
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.589 ± 0.158
4.29 ± 0.245
3.782 ± 0.157
4.056 ± 0.317
5.379 ± 0.201
5.975 ± 0.364
5.588 ± 0.333
6.83 ± 0.366
1.492 ± 0.178
3.5 ± 0.198
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here