Amino acid dipepetide frequency for Enterobacteria phage phiP27

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.727AlaAla: 9.727 ± 0.981
1.098AlaCys: 1.098 ± 0.298
5.099AlaAsp: 5.099 ± 0.595
6.825AlaGlu: 6.825 ± 0.748
3.295AlaPhe: 3.295 ± 0.549
7.531AlaGly: 7.531 ± 0.753
1.412AlaHis: 1.412 ± 0.339
5.099AlaIle: 5.099 ± 0.643
4.236AlaLys: 4.236 ± 0.453
8.472AlaLeu: 8.472 ± 0.766
2.51AlaMet: 2.51 ± 0.396
2.196AlaAsn: 2.196 ± 0.454
2.824AlaPro: 2.824 ± 0.352
3.922AlaGln: 3.922 ± 0.589
6.903AlaArg: 6.903 ± 0.734
4.471AlaSer: 4.471 ± 0.57
4.707AlaThr: 4.707 ± 0.615
5.805AlaVal: 5.805 ± 0.85
1.647AlaTrp: 1.647 ± 0.345
2.353AlaTyr: 2.353 ± 0.382
0.0AlaXaa: 0.0 ± 0.0
Cys
0.863CysAla: 0.863 ± 0.281
0.078CysCys: 0.078 ± 0.083
0.863CysAsp: 0.863 ± 0.241
0.706CysGlu: 0.706 ± 0.251
0.235CysPhe: 0.235 ± 0.111
0.784CysGly: 0.784 ± 0.188
0.392CysHis: 0.392 ± 0.161
0.706CysIle: 0.706 ± 0.285
0.235CysLys: 0.235 ± 0.124
1.412CysLeu: 1.412 ± 0.297
0.235CysMet: 0.235 ± 0.14
0.392CysAsn: 0.392 ± 0.151
0.784CysPro: 0.784 ± 0.239
0.549CysGln: 0.549 ± 0.197
1.02CysArg: 1.02 ± 0.278
0.628CysSer: 0.628 ± 0.232
0.392CysThr: 0.392 ± 0.154
0.863CysVal: 0.863 ± 0.3
0.392CysTrp: 0.392 ± 0.178
0.314CysTyr: 0.314 ± 0.177
0.0CysXaa: 0.0 ± 0.0
Asp
6.275AspAla: 6.275 ± 0.65
0.941AspCys: 0.941 ± 0.256
4.707AspAsp: 4.707 ± 0.607
4.707AspGlu: 4.707 ± 0.486
2.432AspPhe: 2.432 ± 0.415
4.942AspGly: 4.942 ± 0.732
0.628AspHis: 0.628 ± 0.187
2.981AspIle: 2.981 ± 0.515
3.138AspLys: 3.138 ± 0.456
3.844AspLeu: 3.844 ± 0.496
1.255AspMet: 1.255 ± 0.332
2.118AspAsn: 2.118 ± 0.403
3.138AspPro: 3.138 ± 0.596
1.098AspGln: 1.098 ± 0.316
2.275AspArg: 2.275 ± 0.405
3.687AspSer: 3.687 ± 0.451
3.138AspThr: 3.138 ± 0.466
3.922AspVal: 3.922 ± 0.593
0.941AspTrp: 0.941 ± 0.287
2.589AspTyr: 2.589 ± 0.364
0.0AspXaa: 0.0 ± 0.0
Glu
6.354GluAla: 6.354 ± 0.764
0.706GluCys: 0.706 ± 0.246
3.922GluAsp: 3.922 ± 0.499
4.393GluGlu: 4.393 ± 0.727
2.432GluPhe: 2.432 ± 0.427
3.608GluGly: 3.608 ± 0.483
1.334GluHis: 1.334 ± 0.216
4.314GluIle: 4.314 ± 0.572
3.844GluLys: 3.844 ± 0.82
8.55GluLeu: 8.55 ± 0.769
2.275GluMet: 2.275 ± 0.386
2.667GluAsn: 2.667 ± 0.366
2.118GluPro: 2.118 ± 0.399
3.216GluGln: 3.216 ± 0.547
4.001GluArg: 4.001 ± 0.547
3.373GluSer: 3.373 ± 0.413
3.216GluThr: 3.216 ± 0.526
4.628GluVal: 4.628 ± 0.543
1.02GluTrp: 1.02 ± 0.316
2.118GluTyr: 2.118 ± 0.395
0.0GluXaa: 0.0 ± 0.0
Phe
1.804PheAla: 1.804 ± 0.384
0.392PheCys: 0.392 ± 0.181
2.04PheAsp: 2.04 ± 0.365
2.275PheGlu: 2.275 ± 0.414
1.804PhePhe: 1.804 ± 0.319
3.216PheGly: 3.216 ± 0.523
0.471PheHis: 0.471 ± 0.165
2.275PheIle: 2.275 ± 0.493
2.667PheLys: 2.667 ± 0.387
2.118PheLeu: 2.118 ± 0.363
1.255PheMet: 1.255 ± 0.382
2.275PheAsn: 2.275 ± 0.458
1.569PhePro: 1.569 ± 0.311
1.334PheGln: 1.334 ± 0.288
1.961PheArg: 1.961 ± 0.336
2.51PheSer: 2.51 ± 0.479
1.961PheThr: 1.961 ± 0.345
1.647PheVal: 1.647 ± 0.346
0.549PheTrp: 0.549 ± 0.199
1.02PheTyr: 1.02 ± 0.251
0.0PheXaa: 0.0 ± 0.0
Gly
5.726GlyAla: 5.726 ± 0.705
1.255GlyCys: 1.255 ± 0.27
4.707GlyAsp: 4.707 ± 0.636
6.04GlyGlu: 6.04 ± 0.518
2.589GlyPhe: 2.589 ± 0.433
5.57GlyGly: 5.57 ± 0.986
1.02GlyHis: 1.02 ± 0.249
4.314GlyIle: 4.314 ± 0.53
4.314GlyLys: 4.314 ± 0.523
7.138GlyLeu: 7.138 ± 0.754
2.746GlyMet: 2.746 ± 0.429
2.981GlyAsn: 2.981 ± 0.474
1.804GlyPro: 1.804 ± 0.333
1.726GlyGln: 1.726 ± 0.323
4.393GlyArg: 4.393 ± 0.618
4.236GlySer: 4.236 ± 0.502
4.55GlyThr: 4.55 ± 0.661
5.491GlyVal: 5.491 ± 0.765
1.334GlyTrp: 1.334 ± 0.309
2.746GlyTyr: 2.746 ± 0.442
0.0GlyXaa: 0.0 ± 0.0
His
0.941HisAla: 0.941 ± 0.211
0.0HisCys: 0.0 ± 0.0
1.098HisAsp: 1.098 ± 0.302
0.706HisGlu: 0.706 ± 0.194
0.628HisPhe: 0.628 ± 0.231
1.883HisGly: 1.883 ± 0.446
1.02HisHis: 1.02 ± 0.264
1.412HisIle: 1.412 ± 0.353
1.02HisLys: 1.02 ± 0.288
1.883HisLeu: 1.883 ± 0.38
0.549HisMet: 0.549 ± 0.21
0.549HisAsn: 0.549 ± 0.168
0.863HisPro: 0.863 ± 0.291
0.941HisGln: 0.941 ± 0.325
0.941HisArg: 0.941 ± 0.201
1.02HisSer: 1.02 ± 0.322
1.255HisThr: 1.255 ± 0.256
1.412HisVal: 1.412 ± 0.258
0.392HisTrp: 0.392 ± 0.158
0.628HisTyr: 0.628 ± 0.198
0.0HisXaa: 0.0 ± 0.0
Ile
4.864IleAla: 4.864 ± 0.788
1.02IleCys: 1.02 ± 0.247
3.844IleAsp: 3.844 ± 0.61
4.393IleGlu: 4.393 ± 0.596
1.255IlePhe: 1.255 ± 0.361
3.53IleGly: 3.53 ± 0.532
0.784IleHis: 0.784 ± 0.3
3.452IleIle: 3.452 ± 0.445
2.589IleLys: 2.589 ± 0.388
3.687IleLeu: 3.687 ± 0.6
1.098IleMet: 1.098 ± 0.303
3.138IleAsn: 3.138 ± 0.525
1.804IlePro: 1.804 ± 0.373
1.961IleGln: 1.961 ± 0.398
2.902IleArg: 2.902 ± 0.485
5.256IleSer: 5.256 ± 0.837
4.236IleThr: 4.236 ± 0.502
2.432IleVal: 2.432 ± 0.4
0.941IleTrp: 0.941 ± 0.249
1.569IleTyr: 1.569 ± 0.347
0.0IleXaa: 0.0 ± 0.0
Lys
4.864LysAla: 4.864 ± 0.656
0.549LysCys: 0.549 ± 0.204
2.04LysAsp: 2.04 ± 0.383
3.53LysGlu: 3.53 ± 0.621
2.04LysPhe: 2.04 ± 0.445
2.981LysGly: 2.981 ± 0.537
1.726LysHis: 1.726 ± 0.456
3.059LysIle: 3.059 ± 0.441
3.922LysLys: 3.922 ± 0.538
3.844LysLeu: 3.844 ± 0.711
1.569LysMet: 1.569 ± 0.353
3.138LysAsn: 3.138 ± 0.516
2.667LysPro: 2.667 ± 0.517
2.902LysGln: 2.902 ± 0.392
3.608LysArg: 3.608 ± 0.545
3.765LysSer: 3.765 ± 0.492
3.53LysThr: 3.53 ± 0.526
2.981LysVal: 2.981 ± 0.55
0.941LysTrp: 0.941 ± 0.239
0.941LysTyr: 0.941 ± 0.3
0.0LysXaa: 0.0 ± 0.0
Leu
7.687LeuAla: 7.687 ± 0.626
1.255LeuCys: 1.255 ± 0.37
4.079LeuAsp: 4.079 ± 0.451
5.962LeuGlu: 5.962 ± 0.961
3.373LeuPhe: 3.373 ± 0.589
5.413LeuGly: 5.413 ± 0.644
1.334LeuHis: 1.334 ± 0.335
4.785LeuIle: 4.785 ± 0.582
6.197LeuLys: 6.197 ± 0.802
7.295LeuLeu: 7.295 ± 0.874
2.04LeuMet: 2.04 ± 0.314
4.314LeuAsn: 4.314 ± 0.468
4.628LeuPro: 4.628 ± 0.674
2.981LeuGln: 2.981 ± 0.457
6.432LeuArg: 6.432 ± 0.516
5.962LeuSer: 5.962 ± 0.622
5.099LeuThr: 5.099 ± 0.668
5.57LeuVal: 5.57 ± 0.839
1.02LeuTrp: 1.02 ± 0.321
3.53LeuTyr: 3.53 ± 0.481
0.0LeuXaa: 0.0 ± 0.0
Met
2.824MetAla: 2.824 ± 0.47
0.157MetCys: 0.157 ± 0.105
1.255MetAsp: 1.255 ± 0.265
1.255MetGlu: 1.255 ± 0.348
0.549MetPhe: 0.549 ± 0.181
2.275MetGly: 2.275 ± 0.482
0.471MetHis: 0.471 ± 0.179
1.177MetIle: 1.177 ± 0.276
1.804MetLys: 1.804 ± 0.367
2.746MetLeu: 2.746 ± 0.479
1.02MetMet: 1.02 ± 0.24
1.412MetAsn: 1.412 ± 0.27
1.255MetPro: 1.255 ± 0.249
1.255MetGln: 1.255 ± 0.334
1.49MetArg: 1.49 ± 0.311
1.883MetSer: 1.883 ± 0.341
3.216MetThr: 3.216 ± 0.414
1.412MetVal: 1.412 ± 0.262
0.235MetTrp: 0.235 ± 0.139
0.392MetTyr: 0.392 ± 0.167
0.0MetXaa: 0.0 ± 0.0
Asn
4.158AsnAla: 4.158 ± 0.489
0.471AsnCys: 0.471 ± 0.178
2.51AsnAsp: 2.51 ± 0.405
2.196AsnGlu: 2.196 ± 0.348
1.334AsnPhe: 1.334 ± 0.303
3.216AsnGly: 3.216 ± 0.702
0.941AsnHis: 0.941 ± 0.277
2.04AsnIle: 2.04 ± 0.403
1.883AsnLys: 1.883 ± 0.478
3.373AsnLeu: 3.373 ± 0.509
0.863AsnMet: 0.863 ± 0.231
1.255AsnAsn: 1.255 ± 0.325
2.432AsnPro: 2.432 ± 0.47
1.726AsnGln: 1.726 ± 0.386
2.432AsnArg: 2.432 ± 0.375
2.04AsnSer: 2.04 ± 0.409
2.824AsnThr: 2.824 ± 0.652
2.196AsnVal: 2.196 ± 0.303
0.941AsnTrp: 0.941 ± 0.397
1.098AsnTyr: 1.098 ± 0.269
0.0AsnXaa: 0.0 ± 0.0
Pro
3.608ProAla: 3.608 ± 0.467
0.392ProCys: 0.392 ± 0.172
3.452ProAsp: 3.452 ± 0.588
3.373ProGlu: 3.373 ± 0.486
2.118ProPhe: 2.118 ± 0.358
4.158ProGly: 4.158 ± 0.537
0.863ProHis: 0.863 ± 0.341
1.334ProIle: 1.334 ± 0.425
2.04ProLys: 2.04 ± 0.389
2.981ProLeu: 2.981 ± 0.536
1.49ProMet: 1.49 ± 0.308
1.098ProAsn: 1.098 ± 0.255
1.098ProPro: 1.098 ± 0.251
2.04ProGln: 2.04 ± 0.461
1.412ProArg: 1.412 ± 0.299
2.432ProSer: 2.432 ± 0.436
1.334ProThr: 1.334 ± 0.38
3.844ProVal: 3.844 ± 0.647
0.235ProTrp: 0.235 ± 0.128
1.569ProTyr: 1.569 ± 0.301
0.0ProXaa: 0.0 ± 0.0
Gln
3.53GlnAla: 3.53 ± 0.513
0.235GlnCys: 0.235 ± 0.136
1.726GlnAsp: 1.726 ± 0.368
2.432GlnGlu: 2.432 ± 0.526
1.49GlnPhe: 1.49 ± 0.331
2.275GlnGly: 2.275 ± 0.338
1.098GlnHis: 1.098 ± 0.258
1.804GlnIle: 1.804 ± 0.431
2.824GlnLys: 2.824 ± 0.424
3.687GlnLeu: 3.687 ± 0.47
1.412GlnMet: 1.412 ± 0.339
2.353GlnAsn: 2.353 ± 0.372
1.961GlnPro: 1.961 ± 0.361
2.589GlnGln: 2.589 ± 0.438
3.53GlnArg: 3.53 ± 0.474
2.196GlnSer: 2.196 ± 0.414
2.353GlnThr: 2.353 ± 0.47
2.746GlnVal: 2.746 ± 0.399
0.863GlnTrp: 0.863 ± 0.243
1.49GlnTyr: 1.49 ± 0.376
0.0GlnXaa: 0.0 ± 0.0
Arg
4.55ArgAla: 4.55 ± 0.55
0.628ArgCys: 0.628 ± 0.213
4.236ArgAsp: 4.236 ± 0.535
4.393ArgGlu: 4.393 ± 0.559
2.51ArgPhe: 2.51 ± 0.502
3.138ArgGly: 3.138 ± 0.453
1.726ArgHis: 1.726 ± 0.36
3.452ArgIle: 3.452 ± 0.499
3.608ArgLys: 3.608 ± 0.523
5.648ArgLeu: 5.648 ± 0.631
1.49ArgMet: 1.49 ± 0.308
2.432ArgAsn: 2.432 ± 0.602
2.589ArgPro: 2.589 ± 0.445
4.236ArgGln: 4.236 ± 0.636
3.765ArgArg: 3.765 ± 0.563
2.746ArgSer: 2.746 ± 0.431
3.452ArgThr: 3.452 ± 0.522
3.53ArgVal: 3.53 ± 0.436
1.177ArgTrp: 1.177 ± 0.316
2.275ArgTyr: 2.275 ± 0.394
0.0ArgXaa: 0.0 ± 0.0
Ser
5.726SerAla: 5.726 ± 0.733
0.941SerCys: 0.941 ± 0.277
3.608SerAsp: 3.608 ± 0.471
4.079SerGlu: 4.079 ± 0.564
1.647SerPhe: 1.647 ± 0.384
6.746SerGly: 6.746 ± 0.717
1.334SerHis: 1.334 ± 0.312
2.432SerIle: 2.432 ± 0.467
2.196SerLys: 2.196 ± 0.446
6.197SerLeu: 6.197 ± 0.794
2.275SerMet: 2.275 ± 0.387
1.412SerAsn: 1.412 ± 0.313
2.196SerPro: 2.196 ± 0.446
2.353SerGln: 2.353 ± 0.453
3.765SerArg: 3.765 ± 0.574
4.079SerSer: 4.079 ± 0.653
2.275SerThr: 2.275 ± 0.395
4.707SerVal: 4.707 ± 0.698
0.549SerTrp: 0.549 ± 0.201
1.647SerTyr: 1.647 ± 0.458
0.0SerXaa: 0.0 ± 0.0
Thr
6.511ThrAla: 6.511 ± 0.88
0.628ThrCys: 0.628 ± 0.228
3.295ThrAsp: 3.295 ± 0.462
4.001ThrGlu: 4.001 ± 0.658
2.118ThrPhe: 2.118 ± 0.391
5.413ThrGly: 5.413 ± 0.713
0.784ThrHis: 0.784 ± 0.272
3.844ThrIle: 3.844 ± 0.512
1.883ThrLys: 1.883 ± 0.473
5.491ThrLeu: 5.491 ± 0.748
1.334ThrMet: 1.334 ± 0.255
1.804ThrAsn: 1.804 ± 0.3
3.059ThrPro: 3.059 ± 0.496
2.196ThrGln: 2.196 ± 0.347
2.981ThrArg: 2.981 ± 0.397
3.373ThrSer: 3.373 ± 0.547
3.844ThrThr: 3.844 ± 0.641
3.922ThrVal: 3.922 ± 0.552
0.941ThrTrp: 0.941 ± 0.31
1.334ThrTyr: 1.334 ± 0.325
0.0ThrXaa: 0.0 ± 0.0
Val
6.511ValAla: 6.511 ± 0.829
0.471ValCys: 0.471 ± 0.151
3.687ValAsp: 3.687 ± 0.437
3.844ValGlu: 3.844 ± 0.483
2.196ValPhe: 2.196 ± 0.34
4.314ValGly: 4.314 ± 0.546
0.941ValHis: 0.941 ± 0.237
4.393ValIle: 4.393 ± 0.463
4.001ValLys: 4.001 ± 0.541
5.334ValLeu: 5.334 ± 0.582
1.49ValMet: 1.49 ± 0.311
2.824ValAsn: 2.824 ± 0.508
2.196ValPro: 2.196 ± 0.43
2.589ValGln: 2.589 ± 0.553
3.765ValArg: 3.765 ± 0.456
4.079ValSer: 4.079 ± 0.675
4.471ValThr: 4.471 ± 0.606
4.942ValVal: 4.942 ± 0.696
0.784ValTrp: 0.784 ± 0.211
2.353ValTyr: 2.353 ± 0.458
0.0ValXaa: 0.0 ± 0.0
Trp
0.784TrpAla: 0.784 ± 0.221
0.078TrpCys: 0.078 ± 0.083
0.706TrpAsp: 0.706 ± 0.216
1.098TrpGlu: 1.098 ± 0.316
0.471TrpPhe: 0.471 ± 0.162
1.177TrpGly: 1.177 ± 0.336
0.314TrpHis: 0.314 ± 0.15
0.392TrpIle: 0.392 ± 0.197
0.628TrpLys: 0.628 ± 0.181
2.04TrpLeu: 2.04 ± 0.42
0.628TrpMet: 0.628 ± 0.211
0.549TrpAsn: 0.549 ± 0.231
0.706TrpPro: 0.706 ± 0.281
1.098TrpGln: 1.098 ± 0.281
1.726TrpArg: 1.726 ± 0.331
0.941TrpSer: 0.941 ± 0.271
0.706TrpThr: 0.706 ± 0.17
1.098TrpVal: 1.098 ± 0.314
0.157TrpTrp: 0.157 ± 0.118
0.157TrpTyr: 0.157 ± 0.109
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.902TyrAla: 2.902 ± 0.448
0.549TyrCys: 0.549 ± 0.26
1.883TyrAsp: 1.883 ± 0.392
1.883TyrGlu: 1.883 ± 0.359
0.628TyrPhe: 0.628 ± 0.32
2.51TyrGly: 2.51 ± 0.609
0.549TyrHis: 0.549 ± 0.174
1.49TyrIle: 1.49 ± 0.328
1.569TyrLys: 1.569 ± 0.417
3.216TyrLeu: 3.216 ± 0.432
0.471TyrMet: 0.471 ± 0.173
1.02TyrAsn: 1.02 ± 0.263
1.177TyrPro: 1.177 ± 0.313
1.883TyrGln: 1.883 ± 0.344
2.275TyrArg: 2.275 ± 0.384
1.49TyrSer: 1.49 ± 0.352
2.196TyrThr: 2.196 ± 0.453
2.04TyrVal: 2.04 ± 0.447
0.392TyrTrp: 0.392 ± 0.173
1.177TyrTyr: 1.177 ± 0.32
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (12749 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski