Amino acid dipepetide frequency for Salmonella phage SKML-39

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.246AlaAla: 6.246 ± 0.411
0.674AlaCys: 0.674 ± 0.123
4.675AlaAsp: 4.675 ± 0.399
4.797AlaGlu: 4.797 ± 0.376
2.633AlaPhe: 2.633 ± 0.267
5.307AlaGly: 5.307 ± 0.425
1.449AlaHis: 1.449 ± 0.218
4.328AlaIle: 4.328 ± 0.328
4.246AlaLys: 4.246 ± 0.32
6.063AlaLeu: 6.063 ± 0.364
1.898AlaMet: 1.898 ± 0.215
3.266AlaAsn: 3.266 ± 0.281
2.654AlaPro: 2.654 ± 0.251
3.082AlaGln: 3.082 ± 0.334
3.429AlaArg: 3.429 ± 0.248
3.899AlaSer: 3.899 ± 0.298
4.491AlaThr: 4.491 ± 0.328
5.409AlaVal: 5.409 ± 0.373
0.98AlaTrp: 0.98 ± 0.15
2.674AlaTyr: 2.674 ± 0.241
0.0AlaXaa: 0.0 ± 0.0
Cys
0.714CysAla: 0.714 ± 0.134
0.163CysCys: 0.163 ± 0.066
0.817CysAsp: 0.817 ± 0.15
0.694CysGlu: 0.694 ± 0.12
0.388CysPhe: 0.388 ± 0.089
0.674CysGly: 0.674 ± 0.118
0.347CysHis: 0.347 ± 0.082
0.857CysIle: 0.857 ± 0.126
0.674CysLys: 0.674 ± 0.116
0.817CysLeu: 0.817 ± 0.149
0.245CysMet: 0.245 ± 0.068
0.592CysAsn: 0.592 ± 0.099
0.531CysPro: 0.531 ± 0.099
0.306CysGln: 0.306 ± 0.079
0.51CysArg: 0.51 ± 0.106
0.796CysSer: 0.796 ± 0.129
0.531CysThr: 0.531 ± 0.094
1.041CysVal: 1.041 ± 0.138
0.122CysTrp: 0.122 ± 0.047
0.51CysTyr: 0.51 ± 0.116
0.0CysXaa: 0.0 ± 0.0
Asp
4.879AspAla: 4.879 ± 0.343
0.755AspCys: 0.755 ± 0.14
4.021AspAsp: 4.021 ± 0.374
4.083AspGlu: 4.083 ± 0.32
3.205AspPhe: 3.205 ± 0.249
4.899AspGly: 4.899 ± 0.294
1.0AspHis: 1.0 ± 0.131
4.511AspIle: 4.511 ± 0.28
3.858AspLys: 3.858 ± 0.338
5.532AspLeu: 5.532 ± 0.32
1.919AspMet: 1.919 ± 0.213
2.756AspAsn: 2.756 ± 0.261
3.041AspPro: 3.041 ± 0.273
2.245AspGln: 2.245 ± 0.281
2.694AspArg: 2.694 ± 0.252
3.368AspSer: 3.368 ± 0.253
3.674AspThr: 3.674 ± 0.313
4.613AspVal: 4.613 ± 0.298
1.041AspTrp: 1.041 ± 0.147
3.327AspTyr: 3.327 ± 0.262
0.0AspXaa: 0.0 ± 0.0
Glu
4.613GluAla: 4.613 ± 0.426
0.714GluCys: 0.714 ± 0.12
4.062GluAsp: 4.062 ± 0.331
4.695GluGlu: 4.695 ± 0.405
3.021GluPhe: 3.021 ± 0.258
4.246GluGly: 4.246 ± 0.324
1.449GluHis: 1.449 ± 0.181
3.736GluIle: 3.736 ± 0.293
3.286GluLys: 3.286 ± 0.307
6.308GluLeu: 6.308 ± 0.403
2.184GluMet: 2.184 ± 0.184
2.776GluAsn: 2.776 ± 0.266
2.0GluPro: 2.0 ± 0.203
2.735GluGln: 2.735 ± 0.234
3.838GluArg: 3.838 ± 0.29
3.144GluSer: 3.144 ± 0.268
3.797GluThr: 3.797 ± 0.255
4.838GluVal: 4.838 ± 0.318
1.184GluTrp: 1.184 ± 0.142
2.715GluTyr: 2.715 ± 0.222
0.0GluXaa: 0.0 ± 0.0
Phe
2.531PheAla: 2.531 ± 0.275
0.367PheCys: 0.367 ± 0.088
2.735PheAsp: 2.735 ± 0.25
3.327PheGlu: 3.327 ± 0.292
1.694PhePhe: 1.694 ± 0.176
3.103PheGly: 3.103 ± 0.293
0.796PheHis: 0.796 ± 0.115
2.797PheIle: 2.797 ± 0.248
2.572PheLys: 2.572 ± 0.224
3.001PheLeu: 3.001 ± 0.266
1.347PheMet: 1.347 ± 0.159
2.49PheAsn: 2.49 ± 0.244
1.511PhePro: 1.511 ± 0.199
1.572PheGln: 1.572 ± 0.161
2.245PheArg: 2.245 ± 0.217
3.103PheSer: 3.103 ± 0.238
2.674PheThr: 2.674 ± 0.242
2.817PheVal: 2.817 ± 0.214
0.612PheTrp: 0.612 ± 0.126
1.49PheTyr: 1.49 ± 0.178
0.0PheXaa: 0.0 ± 0.0
Gly
4.103GlyAla: 4.103 ± 0.309
0.878GlyCys: 0.878 ± 0.125
3.94GlyAsp: 3.94 ± 0.311
4.613GlyGlu: 4.613 ± 0.324
2.858GlyPhe: 2.858 ± 0.259
5.328GlyGly: 5.328 ± 0.515
1.572GlyHis: 1.572 ± 0.215
4.593GlyIle: 4.593 ± 0.362
5.205GlyLys: 5.205 ± 0.352
5.328GlyLeu: 5.328 ± 0.354
1.939GlyMet: 1.939 ± 0.21
3.368GlyAsn: 3.368 ± 0.369
1.123GlyPro: 1.123 ± 0.147
2.715GlyGln: 2.715 ± 0.219
3.246GlyArg: 3.246 ± 0.241
4.307GlySer: 4.307 ± 0.388
4.062GlyThr: 4.062 ± 0.408
6.205GlyVal: 6.205 ± 0.434
1.327GlyTrp: 1.327 ± 0.164
2.388GlyTyr: 2.388 ± 0.24
0.0GlyXaa: 0.0 ± 0.0
His
1.184HisAla: 1.184 ± 0.17
0.327HisCys: 0.327 ± 0.084
1.306HisAsp: 1.306 ± 0.177
0.653HisGlu: 0.653 ± 0.116
1.082HisPhe: 1.082 ± 0.167
1.164HisGly: 1.164 ± 0.183
0.49HisHis: 0.49 ± 0.117
1.531HisIle: 1.531 ± 0.201
1.266HisLys: 1.266 ± 0.186
1.613HisLeu: 1.613 ± 0.184
0.612HisMet: 0.612 ± 0.093
0.755HisAsn: 0.755 ± 0.122
0.98HisPro: 0.98 ± 0.154
0.674HisGln: 0.674 ± 0.14
1.021HisArg: 1.021 ± 0.138
0.959HisSer: 0.959 ± 0.145
1.123HisThr: 1.123 ± 0.172
1.184HisVal: 1.184 ± 0.172
0.204HisTrp: 0.204 ± 0.077
0.776HisTyr: 0.776 ± 0.136
0.0HisXaa: 0.0 ± 0.0
Ile
4.511IleAla: 4.511 ± 0.298
0.817IleCys: 0.817 ± 0.137
5.511IleAsp: 5.511 ± 0.388
4.634IleGlu: 4.634 ± 0.304
1.858IlePhe: 1.858 ± 0.206
3.919IleGly: 3.919 ± 0.285
1.184IleHis: 1.184 ± 0.178
3.389IleIle: 3.389 ± 0.339
3.613IleLys: 3.613 ± 0.346
3.899IleLeu: 3.899 ± 0.291
1.368IleMet: 1.368 ± 0.18
3.348IleAsn: 3.348 ± 0.332
3.001IlePro: 3.001 ± 0.246
2.694IleGln: 2.694 ± 0.255
3.021IleArg: 3.021 ± 0.245
3.205IleSer: 3.205 ± 0.263
4.103IleThr: 4.103 ± 0.289
3.797IleVal: 3.797 ± 0.225
0.633IleTrp: 0.633 ± 0.109
1.858IleTyr: 1.858 ± 0.198
0.0IleXaa: 0.0 ± 0.0
Lys
4.552LysAla: 4.552 ± 0.45
0.572LysCys: 0.572 ± 0.125
4.389LysAsp: 4.389 ± 0.304
4.083LysGlu: 4.083 ± 0.32
2.919LysPhe: 2.919 ± 0.246
4.409LysGly: 4.409 ± 0.339
1.123LysHis: 1.123 ± 0.169
3.633LysIle: 3.633 ± 0.271
4.47LysLys: 4.47 ± 0.38
5.266LysLeu: 5.266 ± 0.337
2.633LysMet: 2.633 ± 0.279
2.837LysAsn: 2.837 ± 0.2
2.368LysPro: 2.368 ± 0.258
2.388LysGln: 2.388 ± 0.253
3.47LysArg: 3.47 ± 0.31
3.838LysSer: 3.838 ± 0.301
3.491LysThr: 3.491 ± 0.297
4.593LysVal: 4.593 ± 0.329
0.959LysTrp: 0.959 ± 0.12
1.98LysTyr: 1.98 ± 0.213
0.0LysXaa: 0.0 ± 0.0
Leu
6.205LeuAla: 6.205 ± 0.363
0.714LeuCys: 0.714 ± 0.127
5.266LeuAsp: 5.266 ± 0.401
5.022LeuGlu: 5.022 ± 0.32
3.593LeuPhe: 3.593 ± 0.286
5.369LeuGly: 5.369 ± 0.297
1.388LeuHis: 1.388 ± 0.19
3.96LeuIle: 3.96 ± 0.305
6.369LeuLys: 6.369 ± 0.41
6.369LeuLeu: 6.369 ± 0.506
2.062LeuMet: 2.062 ± 0.223
4.266LeuAsn: 4.266 ± 0.277
3.593LeuPro: 3.593 ± 0.273
2.756LeuGln: 2.756 ± 0.256
3.94LeuArg: 3.94 ± 0.29
5.43LeuSer: 5.43 ± 0.323
4.96LeuThr: 4.96 ± 0.322
6.022LeuVal: 6.022 ± 0.376
0.857LeuTrp: 0.857 ± 0.17
3.001LeuTyr: 3.001 ± 0.259
0.0LeuXaa: 0.0 ± 0.0
Met
2.409MetAla: 2.409 ± 0.229
0.367MetCys: 0.367 ± 0.101
1.531MetAsp: 1.531 ± 0.199
1.429MetGlu: 1.429 ± 0.192
1.551MetPhe: 1.551 ± 0.186
1.592MetGly: 1.592 ± 0.173
0.408MetHis: 0.408 ± 0.101
1.674MetIle: 1.674 ± 0.178
2.347MetLys: 2.347 ± 0.242
2.368MetLeu: 2.368 ± 0.231
0.817MetMet: 0.817 ± 0.129
1.449MetAsn: 1.449 ± 0.207
1.021MetPro: 1.021 ± 0.162
1.021MetGln: 1.021 ± 0.139
1.653MetArg: 1.653 ± 0.178
1.98MetSer: 1.98 ± 0.195
1.572MetThr: 1.572 ± 0.182
1.878MetVal: 1.878 ± 0.226
0.265MetTrp: 0.265 ± 0.071
0.878MetTyr: 0.878 ± 0.135
0.0MetXaa: 0.0 ± 0.0
Asn
4.083AsnAla: 4.083 ± 0.312
0.572AsnCys: 0.572 ± 0.109
2.45AsnAsp: 2.45 ± 0.239
2.552AsnGlu: 2.552 ± 0.261
2.143AsnPhe: 2.143 ± 0.202
4.123AsnGly: 4.123 ± 0.319
0.98AsnHis: 0.98 ± 0.147
3.123AsnIle: 3.123 ± 0.269
2.776AsnLys: 2.776 ± 0.197
3.94AsnLeu: 3.94 ± 0.323
1.286AsnMet: 1.286 ± 0.158
2.817AsnAsn: 2.817 ± 0.277
2.47AsnPro: 2.47 ± 0.247
1.531AsnGln: 1.531 ± 0.156
2.327AsnArg: 2.327 ± 0.241
2.817AsnSer: 2.817 ± 0.278
3.082AsnThr: 3.082 ± 0.288
3.613AsnVal: 3.613 ± 0.319
0.633AsnTrp: 0.633 ± 0.119
1.898AsnTyr: 1.898 ± 0.236
0.0AsnXaa: 0.0 ± 0.0
Pro
2.592ProAla: 2.592 ± 0.255
0.449ProCys: 0.449 ± 0.1
3.021ProAsp: 3.021 ± 0.223
3.491ProGlu: 3.491 ± 0.287
1.817ProPhe: 1.817 ± 0.199
2.409ProGly: 2.409 ± 0.22
0.653ProHis: 0.653 ± 0.115
1.858ProIle: 1.858 ± 0.177
2.388ProLys: 2.388 ± 0.206
3.001ProLeu: 3.001 ± 0.28
0.959ProMet: 0.959 ± 0.146
1.694ProAsn: 1.694 ± 0.171
1.204ProPro: 1.204 ± 0.193
1.531ProGln: 1.531 ± 0.208
1.592ProArg: 1.592 ± 0.193
2.878ProSer: 2.878 ± 0.259
2.47ProThr: 2.47 ± 0.202
3.062ProVal: 3.062 ± 0.262
0.592ProTrp: 0.592 ± 0.107
1.286ProTyr: 1.286 ± 0.179
0.0ProXaa: 0.0 ± 0.0
Gln
2.817GlnAla: 2.817 ± 0.272
0.408GlnCys: 0.408 ± 0.098
2.49GlnAsp: 2.49 ± 0.273
2.327GlnGlu: 2.327 ± 0.257
1.837GlnPhe: 1.837 ± 0.185
2.225GlnGly: 2.225 ± 0.227
0.653GlnHis: 0.653 ± 0.127
2.286GlnIle: 2.286 ± 0.19
2.429GlnLys: 2.429 ± 0.256
2.939GlnLeu: 2.939 ± 0.231
1.143GlnMet: 1.143 ± 0.148
1.633GlnAsn: 1.633 ± 0.203
1.347GlnPro: 1.347 ± 0.165
1.715GlnGln: 1.715 ± 0.273
1.858GlnArg: 1.858 ± 0.189
2.368GlnSer: 2.368 ± 0.263
2.307GlnThr: 2.307 ± 0.204
2.776GlnVal: 2.776 ± 0.215
0.531GlnTrp: 0.531 ± 0.111
1.694GlnTyr: 1.694 ± 0.155
0.0GlnXaa: 0.0 ± 0.0
Arg
3.103ArgAla: 3.103 ± 0.265
0.776ArgCys: 0.776 ± 0.144
2.939ArgAsp: 2.939 ± 0.215
3.184ArgGlu: 3.184 ± 0.275
2.062ArgPhe: 2.062 ± 0.253
2.98ArgGly: 2.98 ± 0.262
0.939ArgHis: 0.939 ± 0.155
3.164ArgIle: 3.164 ± 0.25
3.184ArgLys: 3.184 ± 0.289
4.593ArgLeu: 4.593 ± 0.322
1.776ArgMet: 1.776 ± 0.202
2.347ArgAsn: 2.347 ± 0.213
1.837ArgPro: 1.837 ± 0.194
1.939ArgGln: 1.939 ± 0.202
3.001ArgArg: 3.001 ± 0.272
2.899ArgSer: 2.899 ± 0.254
2.572ArgThr: 2.572 ± 0.232
3.164ArgVal: 3.164 ± 0.268
0.755ArgTrp: 0.755 ± 0.132
2.021ArgTyr: 2.021 ± 0.236
0.0ArgXaa: 0.0 ± 0.0
Ser
4.083SerAla: 4.083 ± 0.366
0.469SerCys: 0.469 ± 0.134
3.695SerAsp: 3.695 ± 0.245
3.552SerGlu: 3.552 ± 0.221
2.47SerPhe: 2.47 ± 0.239
4.797SerGly: 4.797 ± 0.458
1.0SerHis: 1.0 ± 0.164
4.083SerIle: 4.083 ± 0.312
3.572SerLys: 3.572 ± 0.301
5.307SerLeu: 5.307 ± 0.327
1.551SerMet: 1.551 ± 0.174
3.552SerAsn: 3.552 ± 0.385
2.164SerPro: 2.164 ± 0.2
2.184SerGln: 2.184 ± 0.221
2.735SerArg: 2.735 ± 0.211
4.042SerSer: 4.042 ± 0.418
3.307SerThr: 3.307 ± 0.342
4.552SerVal: 4.552 ± 0.368
0.551SerTrp: 0.551 ± 0.102
2.409SerTyr: 2.409 ± 0.256
0.0SerXaa: 0.0 ± 0.0
Thr
4.777ThrAla: 4.777 ± 0.38
0.572ThrCys: 0.572 ± 0.127
3.593ThrAsp: 3.593 ± 0.296
3.94ThrGlu: 3.94 ± 0.285
2.674ThrPhe: 2.674 ± 0.252
4.552ThrGly: 4.552 ± 0.364
0.98ThrHis: 0.98 ± 0.162
3.96ThrIle: 3.96 ± 0.307
3.695ThrLys: 3.695 ± 0.267
5.103ThrLeu: 5.103 ± 0.36
0.959ThrMet: 0.959 ± 0.153
2.715ThrAsn: 2.715 ± 0.253
3.389ThrPro: 3.389 ± 0.269
1.96ThrGln: 1.96 ± 0.212
2.98ThrArg: 2.98 ± 0.206
3.123ThrSer: 3.123 ± 0.29
3.776ThrThr: 3.776 ± 0.384
4.613ThrVal: 4.613 ± 0.4
0.735ThrTrp: 0.735 ± 0.129
1.735ThrTyr: 1.735 ± 0.204
0.0ThrXaa: 0.0 ± 0.0
Val
4.838ValAla: 4.838 ± 0.357
0.939ValCys: 0.939 ± 0.15
5.287ValAsp: 5.287 ± 0.32
5.103ValGlu: 5.103 ± 0.382
2.817ValPhe: 2.817 ± 0.271
4.838ValGly: 4.838 ± 0.301
1.204ValHis: 1.204 ± 0.152
4.225ValIle: 4.225 ± 0.281
5.083ValLys: 5.083 ± 0.331
5.328ValLeu: 5.328 ± 0.377
1.919ValMet: 1.919 ± 0.198
3.715ValAsn: 3.715 ± 0.29
2.797ValPro: 2.797 ± 0.228
2.837ValGln: 2.837 ± 0.219
3.123ValArg: 3.123 ± 0.241
4.899ValSer: 4.899 ± 0.381
5.062ValThr: 5.062 ± 0.392
6.328ValVal: 6.328 ± 0.435
1.245ValTrp: 1.245 ± 0.161
3.082ValTyr: 3.082 ± 0.273
0.0ValXaa: 0.0 ± 0.0
Trp
1.102TrpAla: 1.102 ± 0.159
0.265TrpCys: 0.265 ± 0.073
0.919TrpAsp: 0.919 ± 0.166
1.164TrpGlu: 1.164 ± 0.122
0.674TrpPhe: 0.674 ± 0.118
0.735TrpGly: 0.735 ± 0.11
0.225TrpHis: 0.225 ± 0.07
0.653TrpIle: 0.653 ± 0.114
0.959TrpLys: 0.959 ± 0.158
1.429TrpLeu: 1.429 ± 0.176
0.449TrpMet: 0.449 ± 0.109
0.796TrpAsn: 0.796 ± 0.147
0.429TrpPro: 0.429 ± 0.098
0.347TrpGln: 0.347 ± 0.088
0.837TrpArg: 0.837 ± 0.124
0.674TrpSer: 0.674 ± 0.124
0.572TrpThr: 0.572 ± 0.108
1.041TrpVal: 1.041 ± 0.144
0.204TrpTrp: 0.204 ± 0.073
0.429TrpTyr: 0.429 ± 0.105
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.858TyrAla: 2.858 ± 0.243
0.531TyrCys: 0.531 ± 0.102
2.776TyrAsp: 2.776 ± 0.227
2.0TyrGlu: 2.0 ± 0.194
1.49TyrPhe: 1.49 ± 0.161
2.429TyrGly: 2.429 ± 0.261
1.061TyrHis: 1.061 ± 0.159
2.0TyrIle: 2.0 ± 0.208
1.98TyrLys: 1.98 ± 0.185
2.96TyrLeu: 2.96 ± 0.22
1.123TyrMet: 1.123 ± 0.129
2.041TyrAsn: 2.041 ± 0.193
1.49TyrPro: 1.49 ± 0.155
1.551TyrGln: 1.551 ± 0.179
1.715TyrArg: 1.715 ± 0.211
2.347TyrSer: 2.347 ± 0.242
2.205TyrThr: 2.205 ± 0.273
3.123TyrVal: 3.123 ± 0.238
0.469TyrTrp: 0.469 ± 0.089
1.429TyrTyr: 1.429 ± 0.141
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 208 proteins (48990 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski