Eubacterium sp. CAG:115

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium; environmental samples

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2289 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5L9S3|R5L9S3_9FIRM Uncharacterized protein OS=Eubacterium sp. CAG:115 OX=1262878 GN=BN470_00254 PE=4 SV=1
MM1 pKa = 7.91KK2 pKa = 10.17LRR4 pKa = 11.84NIISAVAAAAVAASAASLCAFADD27 pKa = 4.04DD28 pKa = 4.03QPAGYY33 pKa = 10.26VYY35 pKa = 10.35FMAEE39 pKa = 4.11KK40 pKa = 7.79TTLGQGFEE48 pKa = 4.18VEE50 pKa = 4.45PVKK53 pKa = 11.01VPYY56 pKa = 10.68YY57 pKa = 10.31EE58 pKa = 5.06GEE60 pKa = 4.01TGLDD64 pKa = 2.95IVEE67 pKa = 4.22RR68 pKa = 11.84AAEE71 pKa = 4.33IKK73 pKa = 10.02TEE75 pKa = 4.03DD76 pKa = 3.13AGYY79 pKa = 10.35GAFITAFADD88 pKa = 3.39TDD90 pKa = 4.28SNDD93 pKa = 3.16VVLPEE98 pKa = 4.38AVAEE102 pKa = 4.32VCTIASGRR110 pKa = 11.84TEE112 pKa = 4.32EE113 pKa = 4.58GWLSAYY119 pKa = 10.45DD120 pKa = 3.73YY121 pKa = 9.48TAEE124 pKa = 4.16SGWTYY129 pKa = 10.36FVNDD133 pKa = 3.96EE134 pKa = 4.12YY135 pKa = 11.47AQVGIADD142 pKa = 3.98YY143 pKa = 10.03TPADD147 pKa = 3.45GDD149 pKa = 4.05VVVFSFTVYY158 pKa = 10.72GYY160 pKa = 10.9GADD163 pKa = 3.52LGVDD167 pKa = 3.29NSSWGGAAALKK178 pKa = 9.78EE179 pKa = 4.05QVKK182 pKa = 8.12TAEE185 pKa = 4.21LVKK188 pKa = 10.8LFADD192 pKa = 4.84NKK194 pKa = 10.69DD195 pKa = 3.95LLNGSDD201 pKa = 3.52DD202 pKa = 3.31QAYY205 pKa = 10.24AFTAAGEE212 pKa = 4.02ALAQYY217 pKa = 10.21EE218 pKa = 4.57STQEE222 pKa = 4.08DD223 pKa = 3.3VDD225 pKa = 3.95NAVDD229 pKa = 3.51RR230 pKa = 11.84LEE232 pKa = 5.11NILGTEE238 pKa = 4.11TLVTEE243 pKa = 4.35EE244 pKa = 4.22XXXNSCDD251 pKa = 3.25RR252 pKa = 11.84GKK254 pKa = 9.96PLSS257 pKa = 3.67

Molecular weight: 27.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5L4Q6|R5L4Q6_9FIRM Stage 0 sporulation protein A homolog OS=Eubacterium sp. CAG:115 OX=1262878 GN=BN470_00758 PE=4 SV=1
MM1 pKa = 7.2YY2 pKa = 10.32FLLRR6 pKa = 11.84RR7 pKa = 11.84IFLRR11 pKa = 11.84VDD13 pKa = 3.94LARR16 pKa = 11.84DD17 pKa = 3.47TVRR20 pKa = 11.84VSKK23 pKa = 10.86GLLLRR28 pKa = 11.84RR29 pKa = 11.84QFCIPFSAVTKK40 pKa = 9.63VEE42 pKa = 3.88VRR44 pKa = 11.84RR45 pKa = 11.84TPVLRR50 pKa = 11.84LLRR53 pKa = 11.84GKK55 pKa = 9.68RR56 pKa = 11.84VKK58 pKa = 10.4ISTLSGGVSFYY69 pKa = 10.61MKK71 pKa = 10.24AVEE74 pKa = 5.21HH75 pKa = 6.86IPFLPEE81 pKa = 3.2NDD83 pKa = 3.55GAYY86 pKa = 9.52IRR88 pKa = 11.84AKK90 pKa = 9.86PLQSVVGAFVDD101 pKa = 4.22TRR103 pKa = 11.84ALSGVITFGLFLNRR117 pKa = 11.84IGSVFGDD124 pKa = 3.51EE125 pKa = 4.21SFGRR129 pKa = 11.84IMTALAGAAEE139 pKa = 4.7GVTKK143 pKa = 10.79LLAALHH149 pKa = 6.2IGVPRR154 pKa = 11.84VTAFAAVFVGVAWLFVFLGKK174 pKa = 10.47ALGMLRR180 pKa = 11.84FRR182 pKa = 11.84LSCEE186 pKa = 3.8SGFITVQRR194 pKa = 11.84GILTLYY200 pKa = 9.48EE201 pKa = 4.1CRR203 pKa = 11.84LVRR206 pKa = 11.84NNLTGVLRR214 pKa = 11.84CDD216 pKa = 3.44TLTTLLLRR224 pKa = 11.84AAPLYY229 pKa = 10.9AHH231 pKa = 7.36DD232 pKa = 4.57VLLFPPVSRR241 pKa = 11.84RR242 pKa = 11.84TAEE245 pKa = 4.27RR246 pKa = 11.84ILTKK250 pKa = 9.52MCRR253 pKa = 11.84IPPPSGHH260 pKa = 5.95VKK262 pKa = 10.27PPFSAMFGHH271 pKa = 7.15CAAPLGWLGAFTAALLLTFIAEE293 pKa = 4.2PFAAGLVQSVLWCGIVVSLWFTAAYY318 pKa = 9.05AVYY321 pKa = 9.45MRR323 pKa = 11.84LSWLSSCPVEE333 pKa = 3.87TAGISFRR340 pKa = 11.84RR341 pKa = 11.84GARR344 pKa = 11.84LYY346 pKa = 8.84TAVMPAQKK354 pKa = 10.32LAFTITGRR362 pKa = 11.84NIFQRR367 pKa = 11.84FSGMCDD373 pKa = 2.49ITLCSAGRR381 pKa = 11.84KK382 pKa = 7.19QYY384 pKa = 11.05RR385 pKa = 11.84LRR387 pKa = 11.84NVPYY391 pKa = 10.6DD392 pKa = 3.37EE393 pKa = 4.31VRR395 pKa = 11.84RR396 pKa = 11.84IFPPEE401 pKa = 3.68KK402 pKa = 9.88PAA404 pKa = 4.96

Molecular weight: 44.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2289

0

2289

716508

31

2198

313.0

34.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.807 ± 0.067

1.709 ± 0.023

5.925 ± 0.045

6.95 ± 0.051

4.15 ± 0.035

7.568 ± 0.05

1.579 ± 0.024

6.875 ± 0.044

5.796 ± 0.05

8.567 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.905 ± 0.027

4.167 ± 0.04

3.665 ± 0.033

2.8 ± 0.029

4.829 ± 0.045

6.587 ± 0.047

5.581 ± 0.039

7.007 ± 0.04

0.838 ± 0.017

3.691 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski