Amino acid dipepetide frequency for Eubacterium sp. CAG:115

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.189AlaAla: 11.189 ± 0.22	1.299AlaCys: 1.299 ± 0.044	6.084AlaAsp: 6.084 ± 0.101	7.619AlaGlu: 7.619 ± 0.136	3.207AlaPhe: 3.207 ± 0.076	7.049AlaGly: 7.049 ± 0.123	1.218AlaHis: 1.218 ± 0.046	4.645AlaIle: 4.645 ± 0.099	4.969AlaLys: 4.969 ± 0.1	8.039AlaLeu: 8.039 ± 0.114	2.366AlaMet: 2.366 ± 0.067	2.694AlaAsn: 2.694 ± 0.058	2.934AlaPro: 2.934 ± 0.07	2.835AlaGln: 2.835 ± 0.07	3.351AlaArg: 3.351 ± 0.069	4.402AlaSer: 4.402 ± 0.09	2.895AlaThr: 2.895 ± 0.081	8.142AlaVal: 8.142 ± 0.129	0.583AlaTrp: 0.583 ± 0.033	2.543AlaTyr: 2.543 ± 0.057	0.006AlaXaa: 0.006 ± 0.003
Cys	1.655CysAla: 1.655 ± 0.053	0.381CysCys: 0.381 ± 0.028	1.04CysAsp: 1.04 ± 0.042	1.122CysGlu: 1.122 ± 0.04	0.723CysPhe: 0.723 ± 0.037	2.066CysGly: 2.066 ± 0.061	0.251CysHis: 0.251 ± 0.016	1.024CysIle: 1.024 ± 0.038	0.628CysLys: 0.628 ± 0.03	1.119CysLeu: 1.119 ± 0.039	0.419CysMet: 0.419 ± 0.023	0.561CysAsn: 0.561 ± 0.027	0.689CysPro: 0.689 ± 0.04	0.354CysGln: 0.354 ± 0.021	0.978CysArg: 0.978 ± 0.037	1.188CysSer: 1.188 ± 0.039	0.963CysThr: 0.963 ± 0.034	1.22CysVal: 1.22 ± 0.045	0.151CysTrp: 0.151 ± 0.014	0.555CysTyr: 0.555 ± 0.029	0.0CysXaa: 0.0 ± 0.0
Asp	4.074AspAla: 4.074 ± 0.085	0.973AspCys: 0.973 ± 0.038	3.366AspAsp: 3.366 ± 0.087	4.675AspGlu: 4.675 ± 0.085	2.843AspPhe: 2.843 ± 0.066	5.164AspGly: 5.164 ± 0.095	0.794AspHis: 0.794 ± 0.032	5.28AspIle: 5.28 ± 0.088	3.703AspLys: 3.703 ± 0.078	4.114AspLeu: 4.114 ± 0.08	1.813AspMet: 1.813 ± 0.055	2.391AspAsn: 2.391 ± 0.064	2.275AspPro: 2.275 ± 0.058	1.023AspGln: 1.023 ± 0.038	2.734AspArg: 2.734 ± 0.067	3.886AspSer: 3.886 ± 0.084	2.92AspThr: 2.92 ± 0.062	4.015AspVal: 4.015 ± 0.078	0.581AspTrp: 0.581 ± 0.034	2.627AspTyr: 2.627 ± 0.072	0.0AspXaa: 0.0 ± 0.0
Glu	5.417GluAla: 5.417 ± 0.103	0.967GluCys: 0.967 ± 0.036	3.457GluAsp: 3.457 ± 0.07	4.92GluGlu: 4.92 ± 0.107	2.929GluPhe: 2.929 ± 0.068	3.831GluGly: 3.831 ± 0.073	1.287GluHis: 1.287 ± 0.037	5.185GluIle: 5.185 ± 0.083	5.253GluLys: 5.253 ± 0.099	7.049GluLeu: 7.049 ± 0.123	2.258GluMet: 2.258 ± 0.054	3.911GluAsn: 3.911 ± 0.081	2.262GluPro: 2.262 ± 0.064	2.844GluGln: 2.844 ± 0.079	3.517GluArg: 3.517 ± 0.081	3.443GluSer: 3.443 ± 0.069	3.407GluThr: 3.407 ± 0.072	3.989GluVal: 3.989 ± 0.078	0.592GluTrp: 0.592 ± 0.032	2.977GluTyr: 2.977 ± 0.066	0.004GluXaa: 0.004 ± 0.002
Phe	3.408PheAla: 3.408 ± 0.08	0.814PheCys: 0.814 ± 0.041	2.786PheAsp: 2.786 ± 0.067	2.405PheGlu: 2.405 ± 0.059	1.622PhePhe: 1.622 ± 0.05	3.417PheGly: 3.417 ± 0.073	0.554PheHis: 0.554 ± 0.025	2.819PheIle: 2.819 ± 0.082	1.791PheLys: 1.791 ± 0.054	3.32PheLeu: 3.32 ± 0.079	1.139PheMet: 1.139 ± 0.042	1.664PheAsn: 1.664 ± 0.052	1.528PhePro: 1.528 ± 0.044	0.913PheGln: 0.913 ± 0.037	2.234PheArg: 2.234 ± 0.059	3.375PheSer: 3.375 ± 0.075	2.978PheThr: 2.978 ± 0.069	2.829PheVal: 2.829 ± 0.074	0.357PheTrp: 0.357 ± 0.021	1.549PheTyr: 1.549 ± 0.047	0.0PheXaa: 0.0 ± 0.0
Gly	6.469GlyAla: 6.469 ± 0.122	1.514GlyCys: 1.514 ± 0.051	4.133GlyAsp: 4.133 ± 0.081	5.26GlyGlu: 5.26 ± 0.075	3.516GlyPhe: 3.516 ± 0.083	5.959GlyGly: 5.959 ± 0.114	1.181GlyHis: 1.181 ± 0.045	5.788GlyIle: 5.788 ± 0.108	5.031GlyLys: 5.031 ± 0.098	5.877GlyLeu: 5.877 ± 0.103	2.529GlyMet: 2.529 ± 0.066	3.015GlyAsn: 3.015 ± 0.07	0.97GlyPro: 0.97 ± 0.039	1.81GlyGln: 1.81 ± 0.061	3.432GlyArg: 3.432 ± 0.077	5.105GlySer: 5.105 ± 0.094	4.502GlyThr: 4.502 ± 0.094	5.812GlyVal: 5.812 ± 0.092	0.791GlyTrp: 0.791 ± 0.036	2.983GlyTyr: 2.983 ± 0.063	0.001GlyXaa: 0.001 ± 0.001
His	1.126HisAla: 1.126 ± 0.04	0.289HisCys: 0.289 ± 0.023	0.966HisAsp: 0.966 ± 0.039	1.016HisGlu: 1.016 ± 0.039	0.782HisPhe: 0.782 ± 0.035	1.4HisGly: 1.4 ± 0.046	0.356HisHis: 0.356 ± 0.041	1.252HisIle: 1.252 ± 0.042	0.751HisLys: 0.751 ± 0.031	1.157HisLeu: 1.157 ± 0.038	0.373HisMet: 0.373 ± 0.022	0.67HisAsn: 0.67 ± 0.029	0.724HisPro: 0.724 ± 0.03	0.353HisGln: 0.353 ± 0.023	0.811HisArg: 0.811 ± 0.035	1.157HisSer: 1.157 ± 0.043	0.914HisThr: 0.914 ± 0.038	0.918HisVal: 0.918 ± 0.038	0.151HisTrp: 0.151 ± 0.012	0.622HisTyr: 0.622 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	6.029IleAla: 6.029 ± 0.089	1.281IleCys: 1.281 ± 0.039	4.152IleAsp: 4.152 ± 0.077	4.039IleGlu: 4.039 ± 0.087	2.557IlePhe: 2.557 ± 0.066	4.811IleGly: 4.811 ± 0.091	1.003IleHis: 1.003 ± 0.04	4.846IleIle: 4.846 ± 0.099	3.729IleLys: 3.729 ± 0.079	5.454IleLeu: 5.454 ± 0.1	1.905IleMet: 1.905 ± 0.06	2.875IleAsn: 2.875 ± 0.064	3.295IlePro: 3.295 ± 0.068	1.764IleGln: 1.764 ± 0.055	3.306IleArg: 3.306 ± 0.072	5.68IleSer: 5.68 ± 0.106	4.505IleThr: 4.505 ± 0.088	4.715IleVal: 4.715 ± 0.079	0.482IleTrp: 0.482 ± 0.028	2.322IleTyr: 2.322 ± 0.057	0.001IleXaa: 0.001 ± 0.001
Lys	4.798LysAla: 4.798 ± 0.096	0.789LysCys: 0.789 ± 0.037	2.896LysAsp: 2.896 ± 0.07	3.566LysGlu: 3.566 ± 0.074	2.098LysPhe: 2.098 ± 0.053	3.518LysGly: 3.518 ± 0.076	1.013LysHis: 1.013 ± 0.039	3.933LysIle: 3.933 ± 0.083	4.29LysLys: 4.29 ± 0.092	5.866LysLeu: 5.866 ± 0.104	1.743LysMet: 1.743 ± 0.054	2.751LysAsn: 2.751 ± 0.07	2.537LysPro: 2.537 ± 0.072	1.937LysGln: 1.937 ± 0.061	2.97LysArg: 2.97 ± 0.073	3.488LysSer: 3.488 ± 0.074	3.358LysThr: 3.358 ± 0.072	3.384LysVal: 3.384 ± 0.074	0.528LysTrp: 0.528 ± 0.026	2.5LysTyr: 2.5 ± 0.07	0.0LysXaa: 0.0 ± 0.0
Leu	7.171LeuAla: 7.171 ± 0.111	1.834LeuCys: 1.834 ± 0.06	5.216LeuAsp: 5.216 ± 0.088	5.123LeuGlu: 5.123 ± 0.105	3.567LeuPhe: 3.567 ± 0.078	6.01LeuGly: 6.01 ± 0.121	1.489LeuHis: 1.489 ± 0.048	5.431LeuIle: 5.431 ± 0.098	4.922LeuLys: 4.922 ± 0.089	7.599LeuLeu: 7.599 ± 0.147	2.608LeuMet: 2.608 ± 0.069	3.637LeuAsn: 3.637 ± 0.071	3.647LeuPro: 3.647 ± 0.08	1.961LeuGln: 1.961 ± 0.057	4.611LeuArg: 4.611 ± 0.095	6.44LeuSer: 6.44 ± 0.101	5.472LeuThr: 5.472 ± 0.091	5.292LeuVal: 5.292 ± 0.104	0.657LeuTrp: 0.657 ± 0.029	2.976LeuTyr: 2.976 ± 0.07	0.004LeuXaa: 0.004 ± 0.003
Met	2.368MetAla: 2.368 ± 0.064	0.44MetCys: 0.44 ± 0.024	1.447MetAsp: 1.447 ± 0.048	1.77MetGlu: 1.77 ± 0.047	1.117MetPhe: 1.117 ± 0.044	1.933MetGly: 1.933 ± 0.059	0.433MetHis: 0.433 ± 0.026	1.939MetIle: 1.939 ± 0.05	2.099MetLys: 2.099 ± 0.051	2.811MetLeu: 2.811 ± 0.071	0.921MetMet: 0.921 ± 0.037	1.598MetAsn: 1.598 ± 0.043	1.213MetPro: 1.213 ± 0.044	0.895MetGln: 0.895 ± 0.037	1.373MetArg: 1.373 ± 0.042	1.893MetSer: 1.893 ± 0.046	1.954MetThr: 1.954 ± 0.053	1.618MetVal: 1.618 ± 0.05	0.236MetTrp: 0.236 ± 0.019	0.99MetTyr: 0.99 ± 0.038	0.0MetXaa: 0.0 ± 0.0
Asn	3.718AsnAla: 3.718 ± 0.068	0.709AsnCys: 0.709 ± 0.035	2.297AsnAsp: 2.297 ± 0.072	2.547AsnGlu: 2.547 ± 0.054	1.544AsnPhe: 1.544 ± 0.048	3.982AsnGly: 3.982 ± 0.1	0.593AsnHis: 0.593 ± 0.034	3.295AsnIle: 3.295 ± 0.074	2.141AsnLys: 2.141 ± 0.055	2.998AsnLeu: 2.998 ± 0.072	1.199AsnMet: 1.199 ± 0.045	1.8AsnAsn: 1.8 ± 0.055	1.872AsnPro: 1.872 ± 0.054	0.939AsnGln: 0.939 ± 0.038	1.851AsnArg: 1.851 ± 0.051	2.936AsnSer: 2.936 ± 0.073	2.423AsnThr: 2.423 ± 0.058	2.807AsnVal: 2.807 ± 0.068	0.374AsnTrp: 0.374 ± 0.024	1.643AsnTyr: 1.643 ± 0.052	0.001AsnXaa: 0.001 ± 0.001
Pro	3.715ProAla: 3.715 ± 0.083	0.581ProCys: 0.581 ± 0.031	2.796ProAsp: 2.796 ± 0.07	4.121ProGlu: 4.121 ± 0.097	1.524ProPhe: 1.524 ± 0.053	2.488ProGly: 2.488 ± 0.063	0.617ProHis: 0.617 ± 0.03	1.87ProIle: 1.87 ± 0.053	1.869ProLys: 1.869 ± 0.051	3.016ProLeu: 3.016 ± 0.066	0.959ProMet: 0.959 ± 0.038	1.206ProAsn: 1.206 ± 0.04	1.017ProPro: 1.017 ± 0.047	1.295ProGln: 1.295 ± 0.049	1.313ProArg: 1.313 ± 0.047	1.968ProSer: 1.968 ± 0.061	1.717ProThr: 1.717 ± 0.055	3.063ProVal: 3.063 ± 0.065	0.306ProTrp: 0.306 ± 0.019	1.203ProTyr: 1.203 ± 0.045	0.003ProXaa: 0.003 ± 0.002
Gln	2.338GlnAla: 2.338 ± 0.059	0.289GlnCys: 0.289 ± 0.018	1.364GlnAsp: 1.364 ± 0.044	1.899GlnGlu: 1.899 ± 0.062	1.143GlnPhe: 1.143 ± 0.04	1.659GlnGly: 1.659 ± 0.055	0.502GlnHis: 0.502 ± 0.024	1.962GlnIle: 1.962 ± 0.051	1.826GlnLys: 1.826 ± 0.057	2.716GlnLeu: 2.716 ± 0.073	0.868GlnMet: 0.868 ± 0.034	1.368GlnAsn: 1.368 ± 0.051	1.184GlnPro: 1.184 ± 0.043	1.235GlnGln: 1.235 ± 0.055	1.583GlnArg: 1.583 ± 0.049	1.625GlnSer: 1.625 ± 0.05	1.654GlnThr: 1.654 ± 0.049	1.534GlnVal: 1.534 ± 0.045	0.201GlnTrp: 0.201 ± 0.016	1.055GlnTyr: 1.055 ± 0.042	0.0GlnXaa: 0.0 ± 0.0
Arg	3.654ArgAla: 3.654 ± 0.08	0.835ArgCys: 0.835 ± 0.033	2.427ArgAsp: 2.427 ± 0.062	3.837ArgGlu: 3.837 ± 0.082	2.14ArgPhe: 2.14 ± 0.052	3.076ArgGly: 3.076 ± 0.075	0.78ArgHis: 0.78 ± 0.032	3.563ArgIle: 3.563 ± 0.075	2.857ArgLys: 2.857 ± 0.055	4.585ArgLeu: 4.585 ± 0.084	1.477ArgMet: 1.477 ± 0.046	1.936ArgAsn: 1.936 ± 0.056	1.513ArgPro: 1.513 ± 0.047	1.527ArgGln: 1.527 ± 0.051	3.178ArgArg: 3.178 ± 0.077	3.103ArgSer: 3.103 ± 0.066	2.603ArgThr: 2.603 ± 0.064	3.07ArgVal: 3.07 ± 0.074	0.426ArgTrp: 0.426 ± 0.03	1.71ArgTyr: 1.71 ± 0.05	0.0ArgXaa: 0.0 ± 0.0
Ser	6.861SerAla: 6.861 ± 0.112	0.977SerCys: 0.977 ± 0.037	4.354SerAsp: 4.354 ± 0.095	4.808SerGlu: 4.808 ± 0.076	2.652SerPhe: 2.652 ± 0.064	6.675SerGly: 6.675 ± 0.124	0.967SerHis: 0.967 ± 0.037	3.799SerIle: 3.799 ± 0.079	2.903SerLys: 2.903 ± 0.062	5.072SerLeu: 5.072 ± 0.091	1.779SerMet: 1.779 ± 0.045	2.147SerAsn: 2.147 ± 0.055	2.158SerPro: 2.158 ± 0.049	1.805SerGln: 1.805 ± 0.057	3.263SerArg: 3.263 ± 0.07	4.346SerSer: 4.346 ± 0.115	3.411SerThr: 3.411 ± 0.079	5.094SerVal: 5.094 ± 0.096	0.562SerTrp: 0.562 ± 0.037	2.232SerTyr: 2.232 ± 0.062	0.003SerXaa: 0.003 ± 0.002
Thr	6.008ThrAla: 6.008 ± 0.115	0.685ThrCys: 0.685 ± 0.031	3.659ThrAsp: 3.659 ± 0.077	3.877ThrGlu: 3.877 ± 0.074	2.053ThrPhe: 2.053 ± 0.051	4.755ThrGly: 4.755 ± 0.091	0.851ThrHis: 0.851 ± 0.039	3.587ThrIle: 3.587 ± 0.077	2.671ThrLys: 2.671 ± 0.062	4.833ThrLeu: 4.833 ± 0.092	1.393ThrMet: 1.393 ± 0.042	1.937ThrAsn: 1.937 ± 0.068	2.511ThrPro: 2.511 ± 0.056	1.451ThrGln: 1.451 ± 0.046	2.208ThrArg: 2.208 ± 0.055	3.271ThrSer: 3.271 ± 0.074	2.897ThrThr: 2.897 ± 0.083	4.895ThrVal: 4.895 ± 0.088	0.427ThrTrp: 0.427 ± 0.027	1.833ThrTyr: 1.833 ± 0.057	0.001ThrXaa: 0.001 ± 0.001
Val	4.48ValAla: 4.48 ± 0.098	1.548ValCys: 1.548 ± 0.044	3.816ValAsp: 3.816 ± 0.078	4.105ValGlu: 4.105 ± 0.084	3.34ValPhe: 3.34 ± 0.075	4.391ValGly: 4.391 ± 0.089	1.121ValHis: 1.121 ± 0.041	5.391ValIle: 5.391 ± 0.094	4.222ValLys: 4.222 ± 0.095	6.307ValLeu: 6.307 ± 0.131	2.247ValMet: 2.247 ± 0.056	3.248ValAsn: 3.248 ± 0.061	2.79ValPro: 2.79 ± 0.063	1.858ValGln: 1.858 ± 0.048	3.407ValArg: 3.407 ± 0.073	5.262ValSer: 5.262 ± 0.087	4.455ValThr: 4.455 ± 0.089	4.927ValVal: 4.927 ± 0.105	0.582ValTrp: 0.582 ± 0.03	2.574ValTyr: 2.574 ± 0.068	0.0ValXaa: 0.0 ± 0.0
Trp	0.579TrpAla: 0.579 ± 0.027	0.197TrpCys: 0.197 ± 0.019	0.525TrpAsp: 0.525 ± 0.033	0.558TrpGlu: 0.558 ± 0.028	0.458TrpPhe: 0.458 ± 0.026	0.622TrpGly: 0.622 ± 0.035	0.179TrpHis: 0.179 ± 0.018	0.498TrpIle: 0.498 ± 0.029	0.444TrpLys: 0.444 ± 0.027	0.895TrpLeu: 0.895 ± 0.04	0.158TrpMet: 0.158 ± 0.016	0.511TrpAsn: 0.511 ± 0.026	0.12TrpPro: 0.12 ± 0.013	0.315TrpGln: 0.315 ± 0.023	0.371TrpArg: 0.371 ± 0.024	0.518TrpSer: 0.518 ± 0.027	0.451TrpThr: 0.451 ± 0.029	0.516TrpVal: 0.516 ± 0.03	0.105TrpTrp: 0.105 ± 0.012	0.357TrpTyr: 0.357 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.003TyrAla: 3.003 ± 0.074	0.687TyrCys: 0.687 ± 0.034	2.47TyrAsp: 2.47 ± 0.064	2.23TyrGlu: 2.23 ± 0.056	1.689TyrPhe: 1.689 ± 0.051	2.869TyrGly: 2.869 ± 0.071	0.597TyrHis: 0.597 ± 0.03	2.646TyrIle: 2.646 ± 0.065	1.865TyrLys: 1.865 ± 0.057	2.839TyrLeu: 2.839 ± 0.069	0.89TyrMet: 0.89 ± 0.039	1.75TyrAsn: 1.75 ± 0.06	1.405TyrPro: 1.405 ± 0.047	0.888TyrGln: 0.888 ± 0.034	1.796TyrArg: 1.796 ± 0.05	2.783TyrSer: 2.783 ± 0.061	2.327TyrThr: 2.327 ± 0.064	2.227TyrVal: 2.227 ± 0.059	0.286TyrTrp: 0.286 ± 0.02	1.658TyrTyr: 1.658 ± 0.061	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.006XaaAla: 0.006 ± 0.003	0.001XaaCys: 0.001 ± 0.001	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.003XaaPro: 0.003 ± 0.002	0.001XaaGln: 0.001 ± 0.001	0.006XaaArg: 0.006 ± 0.003	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.033XaaXaa: 0.033 ± 0.009

Statistics based on 2289 proteins (716508 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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