Streptococcus phage phi-SsuFJZZ39_rum
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7G3MJG0|A0A7G3MJG0_9CAUD Uncharacterized protein OS=Streptococcus phage phi-SsuFJZZ39_rum OX=2664397 PE=4 SV=1
MM1 pKa = 7.55 NDD3 pKa = 4.25 EE4 pKa = 4.47 INLDD8 pKa = 4.15 KK9 pKa = 10.9 IVLTAIEE16 pKa = 4.56 TEE18 pKa = 4.53 MKK20 pKa = 10.02 CKK22 pKa = 10.42 NYY24 pKa = 10.72 LMLKK28 pKa = 9.49 SHH30 pKa = 7.39 GIDD33 pKa = 3.32 IISDD37 pKa = 3.63 YY38 pKa = 11.14 YY39 pKa = 11.4 YY40 pKa = 10.68 VDD42 pKa = 3.32 EE43 pKa = 5.2 DD44 pKa = 3.92 FAVITFSRR52 pKa = 11.84 NEE54 pKa = 3.94 DD55 pKa = 3.07 KK56 pKa = 9.03 MTIRR60 pKa = 11.84 VEE62 pKa = 3.96 NQEE65 pKa = 4.84 CIVCVEE71 pKa = 3.93 NHH73 pKa = 4.89 VVKK76 pKa = 10.85 AFEE79 pKa = 4.3 SFLEE83 pKa = 4.65 GEE85 pKa = 5.13 DD86 pKa = 3.59 IDD88 pKa = 4.27 DD89 pKa = 4.71 QIEE92 pKa = 4.0 ILIKK96 pKa = 9.26 QLCIGKK102 pKa = 8.17 WQIFIEE108 pKa = 4.4 DD109 pKa = 3.6 VANNSLVDD117 pKa = 4.85 AIQKK121 pKa = 10.32 HH122 pKa = 5.36 GFDD125 pKa = 5.13 DD126 pKa = 4.3 YY127 pKa = 11.97 SNGPDD132 pKa = 3.45 AIIYY136 pKa = 7.4 FF137 pKa = 4.24
Molecular weight: 15.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.106
IPC2_protein 4.164
IPC_protein 4.113
Toseland 3.923
ProMoST 4.253
Dawson 4.088
Bjellqvist 4.24
Wikipedia 3.999
Rodwell 3.948
Grimsley 3.834
Solomon 4.088
Lehninger 4.037
Nozaki 4.202
DTASelect 4.406
Thurlkill 3.961
EMBOSS 4.012
Sillero 4.24
Patrickios 3.071
IPC_peptide 4.088
IPC2_peptide 4.215
IPC2.peptide.svr19 4.142
Protein with the highest isoelectric point:
>tr|A0A7G3MJC1|A0A7G3MJC1_9CAUD Terminase small subunit OS=Streptococcus phage phi-SsuFJZZ39_rum OX=2664397 PE=4 SV=1
MM1 pKa = 7.74 PRR3 pKa = 11.84 RR4 pKa = 11.84 PSTPCKK10 pKa = 10.41 QNGCPNLVPYY20 pKa = 8.51 GQKK23 pKa = 9.9 YY24 pKa = 8.86 CANHH28 pKa = 6.26 KK29 pKa = 10.31 VNHH32 pKa = 5.75 QLDD35 pKa = 3.96 AKK37 pKa = 8.2 STKK40 pKa = 10.13 AKK42 pKa = 10.15 GYY44 pKa = 9.42 NGQWTKK50 pKa = 11.25 ARR52 pKa = 11.84 LRR54 pKa = 11.84 YY55 pKa = 10.0 LKK57 pKa = 9.81 VHH59 pKa = 6.46 PLCVQCKK66 pKa = 9.75 AKK68 pKa = 10.52 GRR70 pKa = 11.84 LTKK73 pKa = 10.1 ATVVDD78 pKa = 5.28 HH79 pKa = 6.23 ITPHH83 pKa = 6.97 RR84 pKa = 11.84 GDD86 pKa = 5.06 QEE88 pKa = 5.0 LFWNQTNWQALCKK101 pKa = 9.93 SCHH104 pKa = 5.64 DD105 pKa = 4.22 RR106 pKa = 11.84 KK107 pKa = 8.93 TKK109 pKa = 8.13 TTDD112 pKa = 2.58 RR113 pKa = 11.84 YY114 pKa = 10.6 VEE116 pKa = 3.73 YY117 pKa = 9.41 TYY119 pKa = 11.18 RR120 pKa = 11.84 FF121 pKa = 3.55
Molecular weight: 14.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.185
IPC2_protein 9.282
IPC_protein 9.224
Toseland 9.926
ProMoST 9.633
Dawson 10.145
Bjellqvist 9.853
Wikipedia 10.306
Rodwell 10.613
Grimsley 10.204
Solomon 10.175
Lehninger 10.145
Nozaki 10.043
DTASelect 9.809
Thurlkill 9.999
EMBOSS 10.335
Sillero 10.087
Patrickios 10.16
IPC_peptide 10.175
IPC2_peptide 8.829
IPC2.peptide.svr19 8.062
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
79
0
79
22191
39
1515
280.9
31.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.034 ± 0.691
0.816 ± 0.112
5.795 ± 0.252
7.809 ± 0.371
4.006 ± 0.286
5.768 ± 0.261
1.766 ± 0.099
6.823 ± 0.296
7.674 ± 0.251
9.067 ± 0.225
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.217 ± 0.136
5.408 ± 0.22
2.771 ± 0.146
4.538 ± 0.227
4.592 ± 0.168
6.624 ± 0.247
6.147 ± 0.397
6.102 ± 0.173
1.176 ± 0.102
3.866 ± 0.26
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here