Streptococcus phage phi-SsuFJZZ39_rum 
Average proteome isoelectric point is 6.19 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|A0A7G3MJG0|A0A7G3MJG0_9CAUD Uncharacterized protein OS=Streptococcus phage phi-SsuFJZZ39_rum OX=2664397 PE=4 SV=1 
MM1 pKa = 7.55  NDD3 pKa = 4.25  EE4 pKa = 4.47  INLDD8 pKa = 4.15  KK9 pKa = 10.9  IVLTAIEE16 pKa = 4.56  TEE18 pKa = 4.53  MKK20 pKa = 10.02  CKK22 pKa = 10.42  NYY24 pKa = 10.72  LMLKK28 pKa = 9.49  SHH30 pKa = 7.39  GIDD33 pKa = 3.32  IISDD37 pKa = 3.63  YY38 pKa = 11.14  YY39 pKa = 11.4  YY40 pKa = 10.68  VDD42 pKa = 3.32  EE43 pKa = 5.2  DD44 pKa = 3.92  FAVITFSRR52 pKa = 11.84  NEE54 pKa = 3.94  DD55 pKa = 3.07  KK56 pKa = 9.03  MTIRR60 pKa = 11.84  VEE62 pKa = 3.96  NQEE65 pKa = 4.84  CIVCVEE71 pKa = 3.93  NHH73 pKa = 4.89  VVKK76 pKa = 10.85  AFEE79 pKa = 4.3  SFLEE83 pKa = 4.65  GEE85 pKa = 5.13  DD86 pKa = 3.59  IDD88 pKa = 4.27  DD89 pKa = 4.71  QIEE92 pKa = 4.0  ILIKK96 pKa = 9.26  QLCIGKK102 pKa = 8.17  WQIFIEE108 pKa = 4.4  DD109 pKa = 3.6  VANNSLVDD117 pKa = 4.85  AIQKK121 pKa = 10.32  HH122 pKa = 5.36  GFDD125 pKa = 5.13  DD126 pKa = 4.3  YY127 pKa = 11.97  SNGPDD132 pKa = 3.45  AIIYY136 pKa = 7.4  FF137 pKa = 4.24   
 Molecular weight: 15.88 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  4.106 
IPC2_protein 4.164 
IPC_protein 4.113 
Toseland    3.923 
ProMoST     4.253 
Dawson      4.088 
Bjellqvist  4.24 
Wikipedia   3.999 
Rodwell     3.948 
Grimsley    3.834 
Solomon     4.088 
Lehninger   4.037 
Nozaki      4.202 
DTASelect   4.406 
Thurlkill   3.961 
EMBOSS      4.012 
Sillero     4.24 
Patrickios  3.071 
IPC_peptide 4.088 
IPC2_peptide  4.215 
IPC2.peptide.svr19  4.142 
 Protein with the highest isoelectric point: 
>tr|A0A7G3MJC1|A0A7G3MJC1_9CAUD Terminase small subunit OS=Streptococcus phage phi-SsuFJZZ39_rum OX=2664397 PE=4 SV=1 
MM1 pKa = 7.74  PRR3 pKa = 11.84  RR4 pKa = 11.84  PSTPCKK10 pKa = 10.41  QNGCPNLVPYY20 pKa = 8.51  GQKK23 pKa = 9.9  YY24 pKa = 8.86  CANHH28 pKa = 6.26  KK29 pKa = 10.31  VNHH32 pKa = 5.75  QLDD35 pKa = 3.96  AKK37 pKa = 8.2  STKK40 pKa = 10.13  AKK42 pKa = 10.15  GYY44 pKa = 9.42  NGQWTKK50 pKa = 11.25  ARR52 pKa = 11.84  LRR54 pKa = 11.84  YY55 pKa = 10.0  LKK57 pKa = 9.81  VHH59 pKa = 6.46  PLCVQCKK66 pKa = 9.75  AKK68 pKa = 10.52  GRR70 pKa = 11.84  LTKK73 pKa = 10.1  ATVVDD78 pKa = 5.28  HH79 pKa = 6.23  ITPHH83 pKa = 6.97  RR84 pKa = 11.84  GDD86 pKa = 5.06  QEE88 pKa = 5.0  LFWNQTNWQALCKK101 pKa = 9.93  SCHH104 pKa = 5.64  DD105 pKa = 4.22  RR106 pKa = 11.84  KK107 pKa = 8.93  TKK109 pKa = 8.13  TTDD112 pKa = 2.58  RR113 pKa = 11.84  YY114 pKa = 10.6  VEE116 pKa = 3.73  YY117 pKa = 9.41  TYY119 pKa = 11.18  RR120 pKa = 11.84  FF121 pKa = 3.55   
 Molecular weight: 14.15 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.185 
IPC2_protein 9.282 
IPC_protein 9.224 
Toseland    9.926 
ProMoST     9.633 
Dawson      10.145 
Bjellqvist  9.853 
Wikipedia   10.306 
Rodwell     10.613 
Grimsley    10.204 
Solomon     10.175 
Lehninger   10.145 
Nozaki      10.043 
DTASelect   9.809 
Thurlkill   9.999 
EMBOSS      10.335 
Sillero     10.087 
Patrickios  10.16 
IPC_peptide 10.175 
IPC2_peptide  8.829 
IPC2.peptide.svr19  8.062 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        79 
 
        
        0
 
        
        79 
         
        22191
 
        39
 
        1515
 
        280.9
 
        31.85
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        7.034 ± 0.691
0.816 ± 0.112
 
        5.795 ± 0.252
7.809 ± 0.371
 
        4.006 ± 0.286
5.768 ± 0.261
 
        1.766 ± 0.099
6.823 ± 0.296
       
        7.674 ± 0.251
9.067 ± 0.225
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        2.217 ± 0.136
5.408 ± 0.22
 
        2.771 ± 0.146
4.538 ± 0.227
 
        4.592 ± 0.168
6.624 ± 0.247
 
        6.147 ± 0.397
6.102 ± 0.173
       
        1.176 ± 0.102
3.866 ± 0.26
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here