Amino acid dipepetide frequency for Shigella flexneri

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.497AlaAla: 9.497 ± 0.124	1.136AlaCys: 1.136 ± 0.033	4.876AlaAsp: 4.876 ± 0.067	6.042AlaGlu: 6.042 ± 0.09	3.583AlaPhe: 3.583 ± 0.048	7.167AlaGly: 7.167 ± 0.095	1.859AlaHis: 1.859 ± 0.046	5.796AlaIle: 5.796 ± 0.088	4.08AlaLys: 4.08 ± 0.062	10.941AlaLeu: 10.941 ± 0.103	3.008AlaMet: 3.008 ± 0.051	3.058AlaAsn: 3.058 ± 0.054	3.521AlaPro: 3.521 ± 0.064	4.282AlaGln: 4.282 ± 0.078	5.331AlaArg: 5.331 ± 0.079	5.248AlaSer: 5.248 ± 0.064	4.664AlaThr: 4.664 ± 0.065	6.569AlaVal: 6.569 ± 0.074	1.517AlaTrp: 1.517 ± 0.041	2.066AlaTyr: 2.066 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	1.033CysAla: 1.033 ± 0.03	0.245CysCys: 0.245 ± 0.016	0.655CysAsp: 0.655 ± 0.024	0.686CysGlu: 0.686 ± 0.023	0.483CysPhe: 0.483 ± 0.019	1.144CysGly: 1.144 ± 0.029	0.373CysHis: 0.373 ± 0.019	0.613CysIle: 0.613 ± 0.024	0.388CysLys: 0.388 ± 0.017	1.053CysLeu: 1.053 ± 0.033	0.247CysMet: 0.247 ± 0.014	0.371CysAsn: 0.371 ± 0.017	0.597CysPro: 0.597 ± 0.023	0.506CysGln: 0.506 ± 0.021	0.698CysArg: 0.698 ± 0.023	0.774CysSer: 0.774 ± 0.027	0.566CysThr: 0.566 ± 0.02	0.776CysVal: 0.776 ± 0.026	0.218CysTrp: 0.218 ± 0.012	0.421CysTyr: 0.421 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	4.935AspAla: 4.935 ± 0.066	0.556AspCys: 0.556 ± 0.022	2.87AspAsp: 2.87 ± 0.055	3.582AspGlu: 3.582 ± 0.052	2.059AspPhe: 2.059 ± 0.045	3.708AspGly: 3.708 ± 0.052	0.957AspHis: 0.957 ± 0.031	3.474AspIle: 3.474 ± 0.05	2.666AspLys: 2.666 ± 0.052	4.623AspLeu: 4.623 ± 0.064	1.279AspMet: 1.279 ± 0.038	2.341AspAsn: 2.341 ± 0.041	2.089AspPro: 2.089 ± 0.045	1.603AspGln: 1.603 ± 0.036	2.594AspArg: 2.594 ± 0.06	2.75AspSer: 2.75 ± 0.05	2.483AspThr: 2.483 ± 0.045	3.776AspVal: 3.776 ± 0.054	0.84AspTrp: 0.84 ± 0.028	1.89AspTyr: 1.89 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	5.203GluAla: 5.203 ± 0.078	0.533GluCys: 0.533 ± 0.022	2.377GluAsp: 2.377 ± 0.048	3.622GluGlu: 3.622 ± 0.068	1.84GluPhe: 1.84 ± 0.038	3.579GluGly: 3.579 ± 0.054	1.506GluHis: 1.506 ± 0.036	3.643GluIle: 3.643 ± 0.066	3.526GluLys: 3.526 ± 0.057	6.309GluLeu: 6.309 ± 0.077	1.945GluMet: 1.945 ± 0.045	2.639GluAsn: 2.639 ± 0.046	2.068GluPro: 2.068 ± 0.041	3.453GluGln: 3.453 ± 0.056	3.861GluArg: 3.861 ± 0.061	3.097GluSer: 3.097 ± 0.05	2.906GluThr: 2.906 ± 0.055	3.837GluVal: 3.837 ± 0.064	0.814GluTrp: 0.814 ± 0.024	1.595GluTyr: 1.595 ± 0.035	0.0GluXaa: 0.0 ± 0.0
Phe	3.722PheAla: 3.722 ± 0.055	0.595PheCys: 0.595 ± 0.021	2.273PheAsp: 2.273 ± 0.041	1.782PheGlu: 1.782 ± 0.039	1.685PhePhe: 1.685 ± 0.041	3.153PheGly: 3.153 ± 0.061	0.807PheHis: 0.807 ± 0.023	2.499PheIle: 2.499 ± 0.045	1.348PheLys: 1.348 ± 0.032	3.315PheLeu: 3.315 ± 0.055	1.04PheMet: 1.04 ± 0.029	1.772PheAsn: 1.772 ± 0.041	1.552PhePro: 1.552 ± 0.037	1.048PheGln: 1.048 ± 0.03	1.916PheArg: 1.916 ± 0.045	3.123PheSer: 3.123 ± 0.051	2.412PheThr: 2.412 ± 0.047	2.566PheVal: 2.566 ± 0.046	0.649PheTrp: 0.649 ± 0.025	1.234PheTyr: 1.234 ± 0.034	0.0PheXaa: 0.0 ± 0.0
Gly	5.934GlyAla: 5.934 ± 0.081	1.066GlyCys: 1.066 ± 0.029	3.644GlyAsp: 3.644 ± 0.059	4.533GlyGlu: 4.533 ± 0.066	3.175GlyPhe: 3.175 ± 0.063	5.145GlyGly: 5.145 ± 0.085	1.621GlyHis: 1.621 ± 0.041	5.034GlyIle: 5.034 ± 0.072	4.161GlyLys: 4.161 ± 0.059	7.291GlyLeu: 7.291 ± 0.086	2.394GlyMet: 2.394 ± 0.047	2.897GlyAsn: 2.897 ± 0.049	1.922GlyPro: 1.922 ± 0.044	2.738GlyGln: 2.738 ± 0.047	3.761GlyArg: 3.761 ± 0.058	4.036GlySer: 4.036 ± 0.055	3.69GlyThr: 3.69 ± 0.066	5.727GlyVal: 5.727 ± 0.065	1.3GlyTrp: 1.3 ± 0.031	2.723GlyTyr: 2.723 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	1.905HisAla: 1.905 ± 0.037	0.341HisCys: 0.341 ± 0.016	1.132HisAsp: 1.132 ± 0.031	1.164HisGlu: 1.164 ± 0.033	1.001HisPhe: 1.001 ± 0.032	1.733HisGly: 1.733 ± 0.036	0.786HisHis: 0.786 ± 0.028	1.379HisIle: 1.379 ± 0.037	0.838HisLys: 0.838 ± 0.025	2.256HisLeu: 2.256 ± 0.049	0.532HisMet: 0.532 ± 0.021	0.946HisAsn: 0.946 ± 0.027	1.33HisPro: 1.33 ± 0.032	1.125HisGln: 1.125 ± 0.029	1.241HisArg: 1.241 ± 0.032	1.306HisSer: 1.306 ± 0.036	1.074HisThr: 1.074 ± 0.031	1.408HisVal: 1.408 ± 0.036	0.435HisTrp: 0.435 ± 0.018	0.973HisTyr: 0.973 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	6.342IleAla: 6.342 ± 0.081	0.779IleCys: 0.779 ± 0.03	3.457IleAsp: 3.457 ± 0.051	3.322IleGlu: 3.322 ± 0.06	2.177IlePhe: 2.177 ± 0.047	4.543IleGly: 4.543 ± 0.073	1.249IleHis: 1.249 ± 0.032	3.614IleIle: 3.614 ± 0.057	2.58IleLys: 2.58 ± 0.044	5.044IleLeu: 5.044 ± 0.076	1.434IleMet: 1.434 ± 0.036	2.834IleAsn: 2.834 ± 0.054	2.838IlePro: 2.838 ± 0.051	1.856IleGln: 1.856 ± 0.039	3.1IleArg: 3.1 ± 0.054	4.048IleSer: 4.048 ± 0.06	3.54IleThr: 3.54 ± 0.048	4.047IleVal: 4.047 ± 0.054	0.71IleTrp: 0.71 ± 0.025	1.565IleTyr: 1.565 ± 0.039	0.0IleXaa: 0.0 ± 0.0
Lys	4.381LysAla: 4.381 ± 0.072	0.318LysCys: 0.318 ± 0.017	2.192LysAsp: 2.192 ± 0.049	2.807LysGlu: 2.807 ± 0.054	1.248LysPhe: 1.248 ± 0.035	3.065LysGly: 3.065 ± 0.053	0.953LysHis: 0.953 ± 0.027	2.553LysIle: 2.553 ± 0.047	2.433LysLys: 2.433 ± 0.056	4.612LysLeu: 4.612 ± 0.076	1.4LysMet: 1.4 ± 0.036	1.997LysAsn: 1.997 ± 0.048	2.26LysPro: 2.26 ± 0.049	2.134LysGln: 2.134 ± 0.039	2.807LysArg: 2.807 ± 0.047	2.515LysSer: 2.515 ± 0.046	2.684LysThr: 2.684 ± 0.052	3.154LysVal: 3.154 ± 0.052	0.504LysTrp: 0.504 ± 0.023	1.245LysTyr: 1.245 ± 0.037	0.0LysXaa: 0.0 ± 0.0
Leu	11.146LeuAla: 11.146 ± 0.116	1.3LeuCys: 1.3 ± 0.034	5.154LeuAsp: 5.154 ± 0.069	5.63LeuGlu: 5.63 ± 0.085	4.152LeuPhe: 4.152 ± 0.064	7.054LeuGly: 7.054 ± 0.087	2.293LeuHis: 2.293 ± 0.047	5.775LeuIle: 5.775 ± 0.084	4.902LeuLys: 4.902 ± 0.066	11.572LeuLeu: 11.572 ± 0.153	3.025LeuMet: 3.025 ± 0.056	4.439LeuAsn: 4.439 ± 0.069	5.73LeuPro: 5.73 ± 0.087	4.076LeuGln: 4.076 ± 0.058	6.227LeuArg: 6.227 ± 0.085	6.942LeuSer: 6.942 ± 0.072	6.235LeuThr: 6.235 ± 0.089	6.935LeuVal: 6.935 ± 0.084	1.449LeuTrp: 1.449 ± 0.037	2.626LeuTyr: 2.626 ± 0.045	0.0LeuXaa: 0.0 ± 0.0
Met	2.983MetAla: 2.983 ± 0.05	0.254MetCys: 0.254 ± 0.015	1.228MetAsp: 1.228 ± 0.03	1.348MetGlu: 1.348 ± 0.034	0.931MetPhe: 0.931 ± 0.026	1.954MetGly: 1.954 ± 0.041	0.513MetHis: 0.513 ± 0.022	1.535MetIle: 1.535 ± 0.037	1.46MetLys: 1.46 ± 0.035	3.283MetLeu: 3.283 ± 0.052	0.903MetMet: 0.903 ± 0.027	1.113MetAsn: 1.113 ± 0.037	1.389MetPro: 1.389 ± 0.035	1.274MetGln: 1.274 ± 0.037	1.561MetArg: 1.561 ± 0.041	1.9MetSer: 1.9 ± 0.038	1.88MetThr: 1.88 ± 0.037	2.03MetVal: 2.03 ± 0.041	0.263MetTrp: 0.263 ± 0.014	0.556MetTyr: 0.556 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	3.647AsnAla: 3.647 ± 0.048	0.395AsnCys: 0.395 ± 0.021	2.081AsnAsp: 2.081 ± 0.04	2.13AsnGlu: 2.13 ± 0.042	1.387AsnPhe: 1.387 ± 0.038	3.324AsnGly: 3.324 ± 0.063	0.9AsnHis: 0.9 ± 0.028	2.539AsnIle: 2.539 ± 0.047	1.753AsnLys: 1.753 ± 0.045	3.727AsnLeu: 3.727 ± 0.06	0.995AsnMet: 0.995 ± 0.028	1.786AsnAsn: 1.786 ± 0.047	2.129AsnPro: 2.129 ± 0.045	1.705AsnGln: 1.705 ± 0.037	2.164AsnArg: 2.164 ± 0.044	2.104AsnSer: 2.104 ± 0.042	2.108AsnThr: 2.108 ± 0.039	2.615AsnVal: 2.615 ± 0.048	0.614AsnTrp: 0.614 ± 0.024	1.211AsnTyr: 1.211 ± 0.037	0.0AsnXaa: 0.0 ± 0.0
Pro	4.127ProAla: 4.127 ± 0.064	0.395ProCys: 0.395 ± 0.021	2.711ProAsp: 2.711 ± 0.044	3.561ProGlu: 3.561 ± 0.05	1.819ProPhe: 1.819 ± 0.038	3.349ProGly: 3.349 ± 0.051	1.047ProHis: 1.047 ± 0.03	1.913ProIle: 1.913 ± 0.047	1.603ProLys: 1.603 ± 0.039	4.959ProLeu: 4.959 ± 0.073	1.092ProMet: 1.092 ± 0.028	1.316ProAsn: 1.316 ± 0.034	1.66ProPro: 1.66 ± 0.038	2.273ProGln: 2.273 ± 0.048	1.999ProArg: 1.999 ± 0.042	2.185ProSer: 2.185 ± 0.046	2.132ProThr: 2.132 ± 0.035	3.828ProVal: 3.828 ± 0.059	0.724ProTrp: 0.724 ± 0.024	1.14ProTyr: 1.14 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	4.29GlnAla: 4.29 ± 0.068	0.406GlnCys: 0.406 ± 0.017	1.678GlnAsp: 1.678 ± 0.038	2.318GlnGlu: 2.318 ± 0.043	1.516GlnPhe: 1.516 ± 0.035	2.858GlnGly: 2.858 ± 0.048	1.252GlnHis: 1.252 ± 0.032	2.317GlnIle: 2.317 ± 0.05	1.951GlnLys: 1.951 ± 0.041	4.977GlnLeu: 4.977 ± 0.07	1.275GlnMet: 1.275 ± 0.033	1.532GlnAsn: 1.532 ± 0.036	2.056GlnPro: 2.056 ± 0.052	3.164GlnGln: 3.164 ± 0.068	3.059GlnArg: 3.059 ± 0.057	2.32GlnSer: 2.32 ± 0.046	2.209GlnThr: 2.209 ± 0.043	2.854GlnVal: 2.854 ± 0.044	0.694GlnTrp: 0.694 ± 0.025	1.272GlnTyr: 1.272 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	4.395ArgAla: 4.395 ± 0.066	0.71ArgCys: 0.71 ± 0.026	3.058ArgAsp: 3.058 ± 0.053	3.902ArgGlu: 3.902 ± 0.068	2.5ArgPhe: 2.5 ± 0.045	3.413ArgGly: 3.413 ± 0.047	1.617ArgHis: 1.617 ± 0.037	3.355ArgIle: 3.355 ± 0.056	2.789ArgLys: 2.789 ± 0.057	6.504ArgLeu: 6.504 ± 0.088	1.636ArgMet: 1.636 ± 0.038	2.184ArgAsn: 2.184 ± 0.04	2.136ArgPro: 2.136 ± 0.044	3.075ArgGln: 3.075 ± 0.054	3.68ArgArg: 3.68 ± 0.059	2.817ArgSer: 2.817 ± 0.052	2.628ArgThr: 2.628 ± 0.048	3.793ArgVal: 3.793 ± 0.056	1.022ArgTrp: 1.022 ± 0.032	2.143ArgTyr: 2.143 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	5.238SerAla: 5.238 ± 0.065	0.718SerCys: 0.718 ± 0.027	3.092SerAsp: 3.092 ± 0.044	3.284SerGlu: 3.284 ± 0.053	2.346SerPhe: 2.346 ± 0.041	5.212SerGly: 5.212 ± 0.071	1.396SerHis: 1.396 ± 0.033	3.062SerIle: 3.062 ± 0.05	2.191SerLys: 2.191 ± 0.045	6.608SerLeu: 6.608 ± 0.076	1.602SerMet: 1.602 ± 0.033	2.002SerAsn: 2.002 ± 0.04	2.559SerPro: 2.559 ± 0.043	2.524SerGln: 2.524 ± 0.044	3.691SerArg: 3.691 ± 0.066	3.536SerSer: 3.536 ± 0.069	2.929SerThr: 2.929 ± 0.056	4.159SerVal: 4.159 ± 0.06	0.96SerTrp: 0.96 ± 0.027	1.619SerTyr: 1.619 ± 0.037	0.0SerXaa: 0.0 ± 0.0
Thr	4.922ThrAla: 4.922 ± 0.063	0.584ThrCys: 0.584 ± 0.022	2.707ThrAsp: 2.707 ± 0.051	2.707ThrGlu: 2.707 ± 0.049	2.111ThrPhe: 2.111 ± 0.036	4.476ThrGly: 4.476 ± 0.057	1.248ThrHis: 1.248 ± 0.029	3.016ThrIle: 3.016 ± 0.048	1.675ThrLys: 1.675 ± 0.037	7.069ThrLeu: 7.069 ± 0.09	1.201ThrMet: 1.201 ± 0.035	1.621ThrAsn: 1.621 ± 0.041	3.077ThrPro: 3.077 ± 0.054	2.157ThrGln: 2.157 ± 0.037	2.969ThrArg: 2.969 ± 0.048	2.89ThrSer: 2.89 ± 0.057	2.923ThrThr: 2.923 ± 0.053	3.939ThrVal: 3.939 ± 0.056	0.836ThrTrp: 0.836 ± 0.025	1.266ThrTyr: 1.266 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	7.02ValAla: 7.02 ± 0.09	0.839ValCys: 0.839 ± 0.027	3.648ValAsp: 3.648 ± 0.059	4.005ValGlu: 4.005 ± 0.063	2.591ValPhe: 2.591 ± 0.046	4.802ValGly: 4.802 ± 0.068	1.282ValHis: 1.282 ± 0.034	4.694ValIle: 4.694 ± 0.064	3.287ValLys: 3.287 ± 0.058	7.157ValLeu: 7.157 ± 0.089	2.213ValMet: 2.213 ± 0.039	2.833ValAsn: 2.833 ± 0.048	2.91ValPro: 2.91 ± 0.046	2.26ValGln: 2.26 ± 0.053	3.652ValArg: 3.652 ± 0.062	4.497ValSer: 4.497 ± 0.068	4.221ValThr: 4.221 ± 0.06	5.58ValVal: 5.58 ± 0.082	1.015ValTrp: 1.015 ± 0.032	1.779ValTyr: 1.779 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	1.033TrpAla: 1.033 ± 0.03	0.243TrpCys: 0.243 ± 0.014	0.62TrpAsp: 0.62 ± 0.021	0.577TrpGlu: 0.577 ± 0.02	0.685TrpPhe: 0.685 ± 0.025	0.941TrpGly: 0.941 ± 0.034	0.493TrpHis: 0.493 ± 0.023	0.753TrpIle: 0.753 ± 0.026	0.584TrpLys: 0.584 ± 0.021	2.297TrpLeu: 2.297 ± 0.046	0.485TrpMet: 0.485 ± 0.018	0.517TrpAsn: 0.517 ± 0.019	0.674TrpPro: 0.674 ± 0.023	1.192TrpGln: 1.192 ± 0.037	1.102TrpArg: 1.102 ± 0.035	0.822TrpSer: 0.822 ± 0.025	0.551TrpThr: 0.551 ± 0.024	0.983TrpVal: 0.983 ± 0.03	0.238TrpTrp: 0.238 ± 0.015	0.452TrpTyr: 0.452 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.487TyrAla: 2.487 ± 0.042	0.436TyrCys: 0.436 ± 0.019	1.621TyrAsp: 1.621 ± 0.042	1.349TyrGlu: 1.349 ± 0.028	1.202TyrPhe: 1.202 ± 0.03	2.177TyrGly: 2.177 ± 0.046	0.753TyrHis: 0.753 ± 0.026	1.513TyrIle: 1.513 ± 0.037	1.047TyrLys: 1.047 ± 0.035	3.065TyrLeu: 3.065 ± 0.048	0.65TyrMet: 0.65 ± 0.024	1.115TyrAsn: 1.115 ± 0.028	1.335TyrPro: 1.335 ± 0.038	1.553TyrGln: 1.553 ± 0.034	1.942TyrArg: 1.942 ± 0.043	1.816TyrSer: 1.816 ± 0.042	1.562TyrThr: 1.562 ± 0.04	1.708TyrVal: 1.708 ± 0.043	0.443TyrTrp: 0.443 ± 0.018	1.0TyrTyr: 1.0 ± 0.032	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4104 proteins (1230400 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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