Euzebya sp. DY32-46

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Nitriliruptoria; Euzebyales; Euzebyaceae; Euzebya

Average proteome isoelectric point is 5.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5625 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346Y6D3|A0A346Y6D3_9ACTN Chaperone protein DnaJ OS=Euzebya sp. DY32-46 OX=1608957 GN=DVS28_b0260 PE=4 SV=1
MM1 pKa = 7.83SDD3 pKa = 4.13LPATTPEE10 pKa = 4.23VIEE13 pKa = 4.78TIPVDD18 pKa = 3.77ADD20 pKa = 3.23MSDD23 pKa = 3.46SYY25 pKa = 11.62TFALIGRR32 pKa = 11.84VNGTDD37 pKa = 3.31ALVGTHH43 pKa = 5.56TTYY46 pKa = 10.79CQPDD50 pKa = 3.7YY51 pKa = 11.48GIDD54 pKa = 3.31HH55 pKa = 7.8WDD57 pKa = 3.31GGMAIADD64 pKa = 3.96YY65 pKa = 11.19APGEE69 pKa = 3.87IVARR73 pKa = 11.84GFGWQDD79 pKa = 2.69GHH81 pKa = 6.89FADD84 pKa = 5.05SCDD87 pKa = 3.84DD88 pKa = 4.04NGDD91 pKa = 3.66GTHH94 pKa = 5.32TVVWTDD100 pKa = 3.18YY101 pKa = 11.84DD102 pKa = 4.39DD103 pKa = 4.43DD104 pKa = 4.32TEE106 pKa = 4.29HH107 pKa = 7.55AMRR110 pKa = 11.84LPVAVDD116 pKa = 3.23GVIFVTLDD124 pKa = 3.07ARR126 pKa = 11.84RR127 pKa = 11.84LAPP130 pKa = 4.15

Molecular weight: 14.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346Y6K1|A0A346Y6K1_9ACTN Uncharacterized protein OS=Euzebya sp. DY32-46 OX=1608957 GN=DVS28_b0328 PE=4 SV=1
MM1 pKa = 7.55LFLAILAVTILALLGARR18 pKa = 11.84WVMSAVKK25 pKa = 10.04PNTGRR30 pKa = 11.84GARR33 pKa = 11.84WVMSAVKK40 pKa = 10.04PNTGRR45 pKa = 11.84GARR48 pKa = 11.84WVMSAVKK55 pKa = 10.04PNTGRR60 pKa = 11.84GARR63 pKa = 11.84WVMSAVKK70 pKa = 10.04PNTGRR75 pKa = 11.84GARR78 pKa = 11.84WVMSAVKK85 pKa = 10.04PNTGRR90 pKa = 11.84GARR93 pKa = 11.84WVMSAAA99 pKa = 3.76

Molecular weight: 10.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5625

0

5625

1909651

37

3356

339.5

36.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.092 ± 0.052

0.694 ± 0.008

7.557 ± 0.031

5.624 ± 0.028

2.619 ± 0.02

9.353 ± 0.032

2.263 ± 0.019

3.7 ± 0.022

1.373 ± 0.021

9.812 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.963 ± 0.015

1.823 ± 0.018

6.001 ± 0.032

2.774 ± 0.016

7.298 ± 0.042

4.986 ± 0.021

6.617 ± 0.034

9.336 ± 0.033

1.463 ± 0.014

1.653 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski