Amino acid dipepetide frequency for Massilia sp. K1S02-61

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.051AlaAla: 18.051 ± 0.186
1.316AlaCys: 1.316 ± 0.032
6.296AlaAsp: 6.296 ± 0.07
6.469AlaGlu: 6.469 ± 0.078
3.998AlaPhe: 3.998 ± 0.054
10.93AlaGly: 10.93 ± 0.098
2.527AlaHis: 2.527 ± 0.048
5.938AlaIle: 5.938 ± 0.067
4.301AlaLys: 4.301 ± 0.077
13.396AlaLeu: 13.396 ± 0.126
3.606AlaMet: 3.606 ± 0.05
3.401AlaAsn: 3.401 ± 0.047
6.17AlaPro: 6.17 ± 0.09
4.97AlaGln: 4.97 ± 0.061
8.623AlaArg: 8.623 ± 0.1
7.041AlaSer: 7.041 ± 0.071
5.95AlaThr: 5.95 ± 0.066
8.675AlaVal: 8.675 ± 0.091
1.668AlaTrp: 1.668 ± 0.038
2.709AlaTyr: 2.709 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
1.255CysAla: 1.255 ± 0.03
0.141CysCys: 0.141 ± 0.009
0.54CysAsp: 0.54 ± 0.019
0.499CysGlu: 0.499 ± 0.018
0.328CysPhe: 0.328 ± 0.015
0.984CysGly: 0.984 ± 0.028
0.245CysHis: 0.245 ± 0.013
0.446CysIle: 0.446 ± 0.019
0.283CysLys: 0.283 ± 0.014
0.797CysLeu: 0.797 ± 0.023
0.235CysMet: 0.235 ± 0.011
0.257CysAsn: 0.257 ± 0.011
0.441CysPro: 0.441 ± 0.017
0.267CysGln: 0.267 ± 0.013
0.601CysArg: 0.601 ± 0.02
0.566CysSer: 0.566 ± 0.023
0.523CysThr: 0.523 ± 0.02
0.668CysVal: 0.668 ± 0.021
0.12CysTrp: 0.12 ± 0.01
0.255CysTyr: 0.255 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
6.883AspAla: 6.883 ± 0.079
0.488AspCys: 0.488 ± 0.019
2.833AspAsp: 2.833 ± 0.045
3.259AspGlu: 3.259 ± 0.049
2.164AspPhe: 2.164 ± 0.04
4.706AspGly: 4.706 ± 0.066
1.039AspHis: 1.039 ± 0.027
2.78AspIle: 2.78 ± 0.043
2.202AspLys: 2.202 ± 0.04
5.155AspLeu: 5.155 ± 0.062
1.453AspMet: 1.453 ± 0.029
1.47AspAsn: 1.47 ± 0.034
2.852AspPro: 2.852 ± 0.041
1.797AspGln: 1.797 ± 0.036
3.142AspArg: 3.142 ± 0.053
2.667AspSer: 2.667 ± 0.047
2.697AspThr: 2.697 ± 0.041
3.93AspVal: 3.93 ± 0.056
0.796AspTrp: 0.796 ± 0.023
1.573AspTyr: 1.573 ± 0.034
0.0AspXaa: 0.0 ± 0.0
Glu
6.443GluAla: 6.443 ± 0.078
0.429GluCys: 0.429 ± 0.017
2.133GluAsp: 2.133 ± 0.037
2.717GluGlu: 2.717 ± 0.052
1.973GluPhe: 1.973 ± 0.034
3.739GluGly: 3.739 ± 0.048
1.308GluHis: 1.308 ± 0.029
2.865GluIle: 2.865 ± 0.046
2.2GluLys: 2.2 ± 0.043
5.884GluLeu: 5.884 ± 0.077
1.468GluMet: 1.468 ± 0.033
1.597GluAsn: 1.597 ± 0.035
2.321GluPro: 2.321 ± 0.047
2.711GluGln: 2.711 ± 0.045
4.406GluArg: 4.406 ± 0.059
2.55GluSer: 2.55 ± 0.047
2.612GluThr: 2.612 ± 0.042
3.886GluVal: 3.886 ± 0.051
0.782GluTrp: 0.782 ± 0.025
1.285GluTyr: 1.285 ± 0.029
0.0GluXaa: 0.0 ± 0.0
Phe
4.317PheAla: 4.317 ± 0.05
0.415PheCys: 0.415 ± 0.02
2.669PheAsp: 2.669 ± 0.045
1.952PheGlu: 1.952 ± 0.035
1.423PhePhe: 1.423 ± 0.034
3.593PheGly: 3.593 ± 0.067
0.788PheHis: 0.788 ± 0.023
1.701PheIle: 1.701 ± 0.036
1.328PheLys: 1.328 ± 0.03
3.031PheLeu: 3.031 ± 0.051
0.93PheMet: 0.93 ± 0.024
1.285PheAsn: 1.285 ± 0.03
1.576PhePro: 1.576 ± 0.03
1.044PheGln: 1.044 ± 0.025
2.046PheArg: 2.046 ± 0.039
2.413PheSer: 2.413 ± 0.039
2.012PheThr: 2.012 ± 0.04
2.627PheVal: 2.627 ± 0.042
0.457PheTrp: 0.457 ± 0.019
1.013PheTyr: 1.013 ± 0.027
0.0PheXaa: 0.0 ± 0.0
Gly
9.642GlyAla: 9.642 ± 0.096
0.889GlyCys: 0.889 ± 0.024
4.13GlyAsp: 4.13 ± 0.059
4.332GlyGlu: 4.332 ± 0.061
3.416GlyPhe: 3.416 ± 0.049
7.071GlyGly: 7.071 ± 0.085
1.87GlyHis: 1.87 ± 0.036
4.339GlyIle: 4.339 ± 0.063
4.057GlyLys: 4.057 ± 0.065
7.925GlyLeu: 7.925 ± 0.071
2.686GlyMet: 2.686 ± 0.044
2.438GlyAsn: 2.438 ± 0.043
2.966GlyPro: 2.966 ± 0.05
3.073GlyGln: 3.073 ± 0.047
5.206GlyArg: 5.206 ± 0.068
4.825GlySer: 4.825 ± 0.064
4.375GlyThr: 4.375 ± 0.057
6.314GlyVal: 6.314 ± 0.068
1.336GlyTrp: 1.336 ± 0.037
2.431GlyTyr: 2.431 ± 0.044
0.0GlyXaa: 0.0 ± 0.0
His
2.741HisAla: 2.741 ± 0.045
0.291HisCys: 0.291 ± 0.015
1.16HisAsp: 1.16 ± 0.03
1.162HisGlu: 1.162 ± 0.03
0.868HisPhe: 0.868 ± 0.023
2.123HisGly: 2.123 ± 0.041
0.588HisHis: 0.588 ± 0.022
1.011HisIle: 1.011 ± 0.025
0.698HisLys: 0.698 ± 0.023
2.067HisLeu: 2.067 ± 0.039
0.538HisMet: 0.538 ± 0.021
0.552HisAsn: 0.552 ± 0.018
1.348HisPro: 1.348 ± 0.029
0.764HisGln: 0.764 ± 0.024
1.3HisArg: 1.3 ± 0.029
1.091HisSer: 1.091 ± 0.027
1.047HisThr: 1.047 ± 0.026
1.526HisVal: 1.526 ± 0.033
0.332HisTrp: 0.332 ± 0.017
0.671HisTyr: 0.671 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
6.455IleAla: 6.455 ± 0.066
0.451IleCys: 0.451 ± 0.017
3.566IleAsp: 3.566 ± 0.048
3.172IleGlu: 3.172 ± 0.056
1.455IlePhe: 1.455 ± 0.036
4.633IleGly: 4.633 ± 0.06
0.932IleHis: 0.932 ± 0.027
1.975IleIle: 1.975 ± 0.045
1.826IleLys: 1.826 ± 0.039
3.898IleLeu: 3.898 ± 0.058
0.981IleMet: 0.981 ± 0.032
1.69IleAsn: 1.69 ± 0.034
2.212IlePro: 2.212 ± 0.043
1.262IleGln: 1.262 ± 0.028
2.798IleArg: 2.798 ± 0.04
2.727IleSer: 2.727 ± 0.044
2.477IleThr: 2.477 ± 0.041
3.743IleVal: 3.743 ± 0.059
0.468IleTrp: 0.468 ± 0.018
1.081IleTyr: 1.081 ± 0.027
0.0IleXaa: 0.0 ± 0.0
Lys
4.449LysAla: 4.449 ± 0.068
0.213LysCys: 0.213 ± 0.011
1.922LysAsp: 1.922 ± 0.04
1.922LysGlu: 1.922 ± 0.044
1.198LysPhe: 1.198 ± 0.028
2.695LysGly: 2.695 ± 0.043
0.778LysHis: 0.778 ± 0.023
1.816LysIle: 1.816 ± 0.042
1.668LysLys: 1.668 ± 0.048
4.156LysLeu: 4.156 ± 0.053
1.122LysMet: 1.122 ± 0.028
1.263LysAsn: 1.263 ± 0.032
2.308LysPro: 2.308 ± 0.047
1.466LysGln: 1.466 ± 0.029
2.631LysArg: 2.631 ± 0.041
2.029LysSer: 2.029 ± 0.038
2.074LysThr: 2.074 ± 0.034
2.981LysVal: 2.981 ± 0.055
0.435LysTrp: 0.435 ± 0.016
0.888LysTyr: 0.888 ± 0.027
0.0LysXaa: 0.0 ± 0.0
Leu
14.081LeuAla: 14.081 ± 0.13
0.959LeuCys: 0.959 ± 0.029
5.969LeuAsp: 5.969 ± 0.07
5.205LeuGlu: 5.205 ± 0.064
3.406LeuPhe: 3.406 ± 0.055
8.018LeuGly: 8.018 ± 0.087
2.147LeuHis: 2.147 ± 0.039
4.231LeuIle: 4.231 ± 0.063
3.902LeuLys: 3.902 ± 0.054
9.809LeuLeu: 9.809 ± 0.111
2.52LeuMet: 2.52 ± 0.045
2.896LeuAsn: 2.896 ± 0.046
5.409LeuPro: 5.409 ± 0.06
3.406LeuGln: 3.406 ± 0.044
6.884LeuArg: 6.884 ± 0.079
5.956LeuSer: 5.956 ± 0.073
4.986LeuThr: 4.986 ± 0.062
7.386LeuVal: 7.386 ± 0.069
1.094LeuTrp: 1.094 ± 0.03
2.164LeuTyr: 2.164 ± 0.036
0.0LeuXaa: 0.0 ± 0.0
Met
3.239MetAla: 3.239 ± 0.054
0.209MetCys: 0.209 ± 0.013
1.242MetAsp: 1.242 ± 0.026
1.266MetGlu: 1.266 ± 0.028
0.902MetPhe: 0.902 ± 0.027
1.871MetGly: 1.871 ± 0.039
0.621MetHis: 0.621 ± 0.019
1.204MetIle: 1.204 ± 0.033
1.233MetLys: 1.233 ± 0.031
2.912MetLeu: 2.912 ± 0.047
0.705MetMet: 0.705 ± 0.023
1.024MetAsn: 1.024 ± 0.03
1.577MetPro: 1.577 ± 0.034
1.118MetGln: 1.118 ± 0.027
1.87MetArg: 1.87 ± 0.037
1.689MetSer: 1.689 ± 0.034
1.667MetThr: 1.667 ± 0.028
1.961MetVal: 1.961 ± 0.039
0.255MetTrp: 0.255 ± 0.014
0.542MetTyr: 0.542 ± 0.023
0.0MetXaa: 0.0 ± 0.0
Asn
3.585AsnAla: 3.585 ± 0.056
0.303AsnCys: 0.303 ± 0.015
1.586AsnAsp: 1.586 ± 0.034
1.414AsnGlu: 1.414 ± 0.033
1.152AsnPhe: 1.152 ± 0.03
2.643AsnGly: 2.643 ± 0.044
0.595AsnHis: 0.595 ± 0.021
1.577AsnIle: 1.577 ± 0.036
1.143AsnLys: 1.143 ± 0.031
2.918AsnLeu: 2.918 ± 0.046
0.717AsnMet: 0.717 ± 0.021
0.983AsnAsn: 0.983 ± 0.029
2.004AsnPro: 2.004 ± 0.032
0.936AsnGln: 0.936 ± 0.025
1.824AsnArg: 1.824 ± 0.034
1.496AsnSer: 1.496 ± 0.039
1.629AsnThr: 1.629 ± 0.031
2.297AsnVal: 2.297 ± 0.04
0.421AsnTrp: 0.421 ± 0.019
0.892AsnTyr: 0.892 ± 0.029
0.0AsnXaa: 0.0 ± 0.0
Pro
6.908ProAla: 6.908 ± 0.083
0.35ProCys: 0.35 ± 0.016
3.095ProAsp: 3.095 ± 0.054
3.25ProGlu: 3.25 ± 0.048
1.776ProPhe: 1.776 ± 0.04
4.493ProGly: 4.493 ± 0.068
1.06ProHis: 1.06 ± 0.028
1.899ProIle: 1.899 ± 0.041
1.665ProLys: 1.665 ± 0.037
4.748ProLeu: 4.748 ± 0.065
1.281ProMet: 1.281 ± 0.031
1.421ProAsn: 1.421 ± 0.028
2.266ProPro: 2.266 ± 0.048
1.822ProGln: 1.822 ± 0.037
2.691ProArg: 2.691 ± 0.044
2.666ProSer: 2.666 ± 0.042
2.24ProThr: 2.24 ± 0.038
4.241ProVal: 4.241 ± 0.06
0.59ProTrp: 0.59 ± 0.021
1.186ProTyr: 1.186 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
4.874GlnAla: 4.874 ± 0.061
0.281GlnCys: 0.281 ± 0.013
1.601GlnAsp: 1.601 ± 0.031
1.701GlnGlu: 1.701 ± 0.037
1.407GlnPhe: 1.407 ± 0.027
2.592GlnGly: 2.592 ± 0.049
0.881GlnHis: 0.881 ± 0.023
1.632GlnIle: 1.632 ± 0.038
1.285GlnLys: 1.285 ± 0.035
4.062GlnLeu: 4.062 ± 0.06
1.028GlnMet: 1.028 ± 0.026
0.955GlnAsn: 0.955 ± 0.026
1.891GlnPro: 1.891 ± 0.035
1.86GlnGln: 1.86 ± 0.045
2.878GlnArg: 2.878 ± 0.046
1.903GlnSer: 1.903 ± 0.037
1.624GlnThr: 1.624 ± 0.034
2.956GlnVal: 2.956 ± 0.047
0.46GlnTrp: 0.46 ± 0.019
0.882GlnTyr: 0.882 ± 0.026
0.0GlnXaa: 0.0 ± 0.0
Arg
7.574ArgAla: 7.574 ± 0.074
0.574ArgCys: 0.574 ± 0.022
3.542ArgAsp: 3.542 ± 0.049
3.819ArgGlu: 3.819 ± 0.053
2.798ArgPhe: 2.798 ± 0.043
4.515ArgGly: 4.515 ± 0.054
1.728ArgHis: 1.728 ± 0.032
3.642ArgIle: 3.642 ± 0.052
2.506ArgLys: 2.506 ± 0.043
6.85ArgLeu: 6.85 ± 0.082
2.017ArgMet: 2.017 ± 0.035
2.073ArgAsn: 2.073 ± 0.036
2.915ArgPro: 2.915 ± 0.04
2.639ArgGln: 2.639 ± 0.045
4.593ArgArg: 4.593 ± 0.066
3.552ArgSer: 3.552 ± 0.05
3.247ArgThr: 3.247 ± 0.047
4.576ArgVal: 4.576 ± 0.057
0.99ArgTrp: 0.99 ± 0.024
1.918ArgTyr: 1.918 ± 0.035
0.0ArgXaa: 0.0 ± 0.0
Ser
6.509SerAla: 6.509 ± 0.071
0.548SerCys: 0.548 ± 0.02
2.876SerAsp: 2.876 ± 0.039
2.689SerGlu: 2.689 ± 0.041
2.245SerPhe: 2.245 ± 0.045
5.471SerGly: 5.471 ± 0.069
1.301SerHis: 1.301 ± 0.033
2.713SerIle: 2.713 ± 0.048
2.005SerLys: 2.005 ± 0.039
5.572SerLeu: 5.572 ± 0.068
1.574SerMet: 1.574 ± 0.033
1.649SerAsn: 1.649 ± 0.037
2.685SerPro: 2.685 ± 0.043
1.827SerGln: 1.827 ± 0.037
3.468SerArg: 3.468 ± 0.047
3.248SerSer: 3.248 ± 0.054
2.96SerThr: 2.96 ± 0.05
4.196SerVal: 4.196 ± 0.056
0.753SerTrp: 0.753 ± 0.02
1.587SerTyr: 1.587 ± 0.035
0.0SerXaa: 0.0 ± 0.0
Thr
5.808ThrAla: 5.808 ± 0.063
0.436ThrCys: 0.436 ± 0.017
2.545ThrAsp: 2.545 ± 0.043
2.492ThrGlu: 2.492 ± 0.038
1.822ThrPhe: 1.822 ± 0.034
4.797ThrGly: 4.797 ± 0.064
1.072ThrHis: 1.072 ± 0.024
2.654ThrIle: 2.654 ± 0.045
1.468ThrLys: 1.468 ± 0.036
5.652ThrLeu: 5.652 ± 0.055
1.324ThrMet: 1.324 ± 0.028
1.435ThrAsn: 1.435 ± 0.036
3.09ThrPro: 3.09 ± 0.052
1.685ThrGln: 1.685 ± 0.03
3.225ThrArg: 3.225 ± 0.048
2.85ThrSer: 2.85 ± 0.049
2.732ThrThr: 2.732 ± 0.044
4.182ThrVal: 4.182 ± 0.062
0.655ThrTrp: 0.655 ± 0.02
1.191ThrTyr: 1.191 ± 0.033
0.0ThrXaa: 0.0 ± 0.0
Val
9.264ValAla: 9.264 ± 0.083
0.735ValCys: 0.735 ± 0.025
4.094ValAsp: 4.094 ± 0.065
4.158ValGlu: 4.158 ± 0.053
2.675ValPhe: 2.675 ± 0.046
5.429ValGly: 5.429 ± 0.065
1.52ValHis: 1.52 ± 0.031
3.703ValIle: 3.703 ± 0.055
2.785ValLys: 2.785 ± 0.052
7.473ValLeu: 7.473 ± 0.075
1.961ValMet: 1.961 ± 0.038
2.432ValAsn: 2.432 ± 0.05
3.773ValPro: 3.773 ± 0.048
2.518ValGln: 2.518 ± 0.041
4.973ValArg: 4.973 ± 0.056
4.312ValSer: 4.312 ± 0.054
4.248ValThr: 4.248 ± 0.061
5.84ValVal: 5.84 ± 0.079
0.87ValTrp: 0.87 ± 0.026
1.729ValTyr: 1.729 ± 0.035
0.0ValXaa: 0.0 ± 0.0
Trp
1.128TrpAla: 1.128 ± 0.028
0.14TrpCys: 0.14 ± 0.01
0.639TrpAsp: 0.639 ± 0.021
0.584TrpGlu: 0.584 ± 0.02
0.552TrpPhe: 0.552 ± 0.017
0.807TrpGly: 0.807 ± 0.022
0.353TrpHis: 0.353 ± 0.017
0.666TrpIle: 0.666 ± 0.023
0.541TrpLys: 0.541 ± 0.021
1.592TrpLeu: 1.592 ± 0.04
0.442TrpMet: 0.442 ± 0.021
0.516TrpAsn: 0.516 ± 0.02
0.582TrpPro: 0.582 ± 0.021
0.611TrpGln: 0.611 ± 0.023
1.044TrpArg: 1.044 ± 0.029
0.823TrpSer: 0.823 ± 0.025
0.689TrpThr: 0.689 ± 0.022
0.838TrpVal: 0.838 ± 0.025
0.198TrpTrp: 0.198 ± 0.011
0.315TrpTyr: 0.315 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.83TyrAla: 2.83 ± 0.044
0.283TyrCys: 0.283 ± 0.013
1.446TyrAsp: 1.446 ± 0.031
1.244TyrGlu: 1.244 ± 0.026
1.06TyrPhe: 1.06 ± 0.028
2.212TyrGly: 2.212 ± 0.037
0.526TyrHis: 0.526 ± 0.019
1.011TyrIle: 1.011 ± 0.028
0.917TyrLys: 0.917 ± 0.027
2.531TyrLeu: 2.531 ± 0.044
0.52TyrMet: 0.52 ± 0.018
0.776TyrAsn: 0.776 ± 0.024
1.264TyrPro: 1.264 ± 0.028
0.979TyrGln: 0.979 ± 0.025
1.866TyrArg: 1.866 ± 0.035
1.473TyrSer: 1.473 ± 0.031
1.326TyrThr: 1.326 ± 0.035
1.667TyrVal: 1.667 ± 0.034
0.381TyrTrp: 0.381 ± 0.017
0.715TyrTyr: 0.715 ± 0.026
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4570 proteins (1497045 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski