Mycobacterium phage CRB2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bclasvirinae; unclassified Bclasvirinae

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A455M871|A0A455M871_9CAUD Uncharacterized protein OS=Mycobacterium phage CRB2 OX=2483623 GN=CRB2_45 PE=4 SV=1
MM1 pKa = 7.48TSPAGAMPYY10 pKa = 9.83TYY12 pKa = 10.95GLQFEE17 pKa = 4.45QLQDD21 pKa = 3.71PSDD24 pKa = 4.16PDD26 pKa = 2.96SWATVTAPMPDD37 pKa = 3.28EE38 pKa = 4.4ATAEE42 pKa = 4.2SQIAYY47 pKa = 7.51YY48 pKa = 11.01ANYY51 pKa = 9.81FGEE54 pKa = 4.31SARR57 pKa = 11.84NFTVVRR63 pKa = 11.84APSPDD68 pKa = 2.83WFDD71 pKa = 3.47WNEE74 pKa = 3.72GDD76 pKa = 3.97PTDD79 pKa = 4.59DD80 pKa = 3.89AVFSFGWSYY89 pKa = 8.51TAPVYY94 pKa = 10.24PYY96 pKa = 8.83TVPPTYY102 pKa = 8.3EE103 pKa = 3.96TQIAPAISYY112 pKa = 9.81EE113 pKa = 3.98QMQGGWAEE121 pKa = 3.93NQARR125 pKa = 11.84PEE127 pKa = 4.1EE128 pKa = 4.17EE129 pKa = 4.17RR130 pKa = 11.84MTDD133 pKa = 3.49LKK135 pKa = 10.64RR136 pKa = 11.84IYY138 pKa = 9.49AAKK141 pKa = 10.6VEE143 pKa = 4.24WQPVPTTPSQQ153 pKa = 3.05

Molecular weight: 17.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A455LM40|A0A455LM40_9CAUD Portal protein OS=Mycobacterium phage CRB2 OX=2483623 GN=CRB2_11 PE=4 SV=1
MM1 pKa = 7.57ARR3 pKa = 11.84RR4 pKa = 11.84GPASARR10 pKa = 11.84RR11 pKa = 11.84GSRR14 pKa = 11.84RR15 pKa = 11.84SMARR19 pKa = 11.84SAPKK23 pKa = 8.95IARR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84GTAVAMSNQTGGIGQFSRR46 pKa = 11.84RR47 pKa = 11.84GGHH50 pKa = 4.68GTYY53 pKa = 10.22HH54 pKa = 6.56GFKK57 pKa = 10.42SKK59 pKa = 10.65KK60 pKa = 5.56QWRR63 pKa = 11.84WAFASKK69 pKa = 10.47QPWAHH74 pKa = 6.09EE75 pKa = 3.91KK76 pKa = 10.28AHH78 pKa = 5.66RR79 pKa = 11.84TKK81 pKa = 10.73GGPKK85 pKa = 8.98VRR87 pKa = 11.84YY88 pKa = 9.16RR89 pKa = 11.84RR90 pKa = 11.84LPASKK95 pKa = 10.38HH96 pKa = 5.76SGHH99 pKa = 6.61KK100 pKa = 10.08GSRR103 pKa = 11.84VPSS106 pKa = 3.7

Molecular weight: 11.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

96

0

96

22949

44

2160

239.1

25.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.933 ± 0.357

0.959 ± 0.121

6.0 ± 0.261

6.27 ± 0.362

2.514 ± 0.106

9.621 ± 0.61

2.074 ± 0.146

4.593 ± 0.15

3.046 ± 0.194

8.497 ± 0.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.384 ± 0.148

2.641 ± 0.149

6.462 ± 0.266

3.477 ± 0.209

6.51 ± 0.366

5.395 ± 0.188

5.878 ± 0.188

6.693 ± 0.178

2.009 ± 0.148

2.044 ± 0.117
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski