American dog tick phlebovirus
Average proteome isoelectric point is 7.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A076E9Z2|A0A076E9Z2_9VIRU Nucleocapsid protein OS=American dog tick phlebovirus OX=1517960 PE=4 SV=1
MM1 pKa = 7.61 SNPSSLAVLVGQNPPPNDD19 pKa = 3.15 EE20 pKa = 5.4 RR21 pKa = 11.84 FFCPGPNVYY30 pKa = 10.67 YY31 pKa = 10.72 YY32 pKa = 8.61 DD33 pKa = 3.72 TQDD36 pKa = 3.2 QVIPHH41 pKa = 5.92 HH42 pKa = 5.64 TSVRR46 pKa = 11.84 RR47 pKa = 11.84 GAFIDD52 pKa = 3.65 VTFEE56 pKa = 3.82 TSALTGSTGSSIVQAPKK73 pKa = 10.38 SQLTVSLSHH82 pKa = 7.15 KK83 pKa = 9.22 YY84 pKa = 9.8 LHH86 pKa = 7.29 DD87 pKa = 3.6 FTFHH91 pKa = 6.56 HH92 pKa = 6.66 LSEE95 pKa = 4.52 RR96 pKa = 11.84 TDD98 pKa = 2.95 SRR100 pKa = 11.84 LRR102 pKa = 11.84 DD103 pKa = 3.51 TFKK106 pKa = 10.61 TVNDD110 pKa = 5.12 GYY112 pKa = 11.2 DD113 pKa = 2.85 NHH115 pKa = 6.67 TPDD118 pKa = 4.3 VIRR121 pKa = 11.84 SEE123 pKa = 3.87 PSGRR127 pKa = 11.84 VVAIEE132 pKa = 3.69 FTTTRR137 pKa = 11.84 KK138 pKa = 9.49 NKK140 pKa = 8.98 QRR142 pKa = 11.84 SVMEE146 pKa = 4.27 AYY148 pKa = 9.24 KK149 pKa = 10.82 KK150 pKa = 10.98 KK151 pKa = 9.48 MGKK154 pKa = 9.47 YY155 pKa = 9.73 CPPCEE160 pKa = 4.18 TRR162 pKa = 11.84 STDD165 pKa = 3.09 KK166 pKa = 10.72 PLSLFCIAVSEE177 pKa = 4.49 SMVVSNLILEE187 pKa = 4.29 QADD190 pKa = 3.67 VNEE193 pKa = 3.93 LCFRR197 pKa = 11.84 FNAAVDD203 pKa = 4.04 VYY205 pKa = 11.57 SFLQRR210 pKa = 11.84 TDD212 pKa = 3.33 VIPRR216 pKa = 11.84 LDD218 pKa = 4.09 DD219 pKa = 4.16 GEE221 pKa = 4.53 TTSIANQVDD230 pKa = 3.58 ATFQDD235 pKa = 3.44 IQFDD239 pKa = 3.73 WKK241 pKa = 9.44 VTEE244 pKa = 4.28 EE245 pKa = 4.12 KK246 pKa = 10.59 FPPFSKK252 pKa = 10.21 EE253 pKa = 4.78 LYY255 pKa = 9.98 DD256 pKa = 3.25 IWSSDD261 pKa = 3.94 DD262 pKa = 3.43 YY263 pKa = 11.69 DD264 pKa = 4.1 LEE266 pKa = 4.77 YY267 pKa = 9.38 IQKK270 pKa = 9.36 MMVHH274 pKa = 5.63 CTEE277 pKa = 4.39 RR278 pKa = 11.84 ACSKK282 pKa = 10.74 LVDD285 pKa = 3.6 DD286 pKa = 5.57 HH287 pKa = 6.99 LIKK290 pKa = 10.04 MDD292 pKa = 3.67 IPRR295 pKa = 11.84 KK296 pKa = 9.6 AKK298 pKa = 10.51 LDD300 pKa = 3.35 ISFKK304 pKa = 9.95 KK305 pKa = 9.04 TLEE308 pKa = 4.11 SVGKK312 pKa = 9.74 YY313 pKa = 9.53 RR314 pKa = 11.84 EE315 pKa = 4.01 NFTQHH320 pKa = 5.53 GKK322 pKa = 8.31 MRR324 pKa = 11.84 SPNYY328 pKa = 9.57 HH329 pKa = 7.02 KK330 pKa = 10.5 STIPFPAVVPRR341 pKa = 11.84 VSRR344 pKa = 11.84 VASISIKK351 pKa = 10.59 DD352 pKa = 3.66 LVRR355 pKa = 11.84 GKK357 pKa = 10.86 EE358 pKa = 3.98 DD359 pKa = 3.57 FPEE362 pKa = 4.23 VKK364 pKa = 10.44 NATGKK369 pKa = 9.9 LWRR372 pKa = 11.84 EE373 pKa = 3.88 VFKK376 pKa = 10.93 AVAMGRR382 pKa = 11.84 VKK384 pKa = 10.36 RR385 pKa = 11.84 VEE387 pKa = 3.88 EE388 pKa = 4.15 DD389 pKa = 3.26 QQTEE393 pKa = 3.97 EE394 pKa = 4.22 AFLMGEE400 pKa = 4.11 VTKK403 pKa = 10.67 DD404 pKa = 3.34 AEE406 pKa = 4.53 HH407 pKa = 6.35 TASAMRR413 pKa = 11.84 TSFHH417 pKa = 6.62 RR418 pKa = 11.84 VTVKK422 pKa = 10.63 LDD424 pKa = 2.9 GDD426 pKa = 3.93 ARR428 pKa = 11.84 VEE430 pKa = 3.88 LATLGVEE437 pKa = 4.88 GKK439 pKa = 9.62 HH440 pKa = 5.84 LAHH443 pKa = 6.8 EE444 pKa = 4.35 PLVKK448 pKa = 10.41 EE449 pKa = 3.95 NRR451 pKa = 11.84 SLKK454 pKa = 10.72 KK455 pKa = 10.34 KK456 pKa = 10.15 GFPLNTDD463 pKa = 2.79 VSDD466 pKa = 3.62 INGYY470 pKa = 10.66 LSDD473 pKa = 4.18 TKK475 pKa = 11.04 DD476 pKa = 3.13 GLLDD480 pKa = 3.56 HH481 pKa = 6.95 SVVGMPEE488 pKa = 3.7 HH489 pKa = 6.62 MEE491 pKa = 5.43 DD492 pKa = 3.65 ILKK495 pKa = 11.02 ANIKK499 pKa = 10.56 SMEE502 pKa = 3.73 AHH504 pKa = 6.55 GFMEE508 pKa = 5.01 SDD510 pKa = 4.29 LPLMDD515 pKa = 3.41 QVSSFWEE522 pKa = 4.32 SPLMAWCQMVTAIGIEE538 pKa = 3.73 LAAASKK544 pKa = 7.25 QHH546 pKa = 6.16 CKK548 pKa = 10.51 GGEE551 pKa = 4.06 FIVKK555 pKa = 10.23 KK556 pKa = 10.13 LRR558 pKa = 11.84 HH559 pKa = 5.54 YY560 pKa = 10.93 DD561 pKa = 2.83 IFLVIKK567 pKa = 7.22 PTRR570 pKa = 11.84 SDD572 pKa = 2.43 SHH574 pKa = 6.17 MFVSLAWYY582 pKa = 9.96 KK583 pKa = 11.34 DD584 pKa = 4.47 DD585 pKa = 4.88 IDD587 pKa = 4.56 CSPLSSSMVFKK598 pKa = 10.87 SSHH601 pKa = 5.89 CQGGWCWTEE610 pKa = 3.38 FHH612 pKa = 6.65 SFKK615 pKa = 10.11 PSKK618 pKa = 10.31 ISTLVRR624 pKa = 11.84 TASTMNNLYY633 pKa = 9.73 WFWRR637 pKa = 11.84 EE638 pKa = 3.81 QYY640 pKa = 10.34 DD641 pKa = 3.61 IKK643 pKa = 10.83 PWDD646 pKa = 3.93 SFPDD650 pKa = 3.97 PLIMASVSKK659 pKa = 10.25 MMKK662 pKa = 9.93 LSLMVMLEE670 pKa = 4.08 DD671 pKa = 3.87 KK672 pKa = 10.99 AATEE676 pKa = 4.11 EE677 pKa = 4.35 LCTNLRR683 pKa = 11.84 YY684 pKa = 8.12 TLLEE688 pKa = 4.15 GFVSEE693 pKa = 4.74 PCLPLPGKK701 pKa = 9.75 MIEE704 pKa = 4.51 KK705 pKa = 9.56 LPSVARR711 pKa = 11.84 TKK713 pKa = 10.65 LQVWLIQKK721 pKa = 9.63 SLSCMCRR728 pKa = 11.84 IAHH731 pKa = 6.07 QPFEE735 pKa = 4.3 PRR737 pKa = 11.84 AKK739 pKa = 9.73 DD740 pKa = 4.14 GRR742 pKa = 11.84 MIWDD746 pKa = 3.74 GLYY749 pKa = 10.99 NPFTEE754 pKa = 4.88 EE755 pKa = 4.25 PVTDD759 pKa = 4.43 PYY761 pKa = 11.59 RR762 pKa = 11.84 LVNLMYY768 pKa = 10.8 LGYY771 pKa = 10.48 LKK773 pKa = 10.96 NKK775 pKa = 10.18 DD776 pKa = 3.52 EE777 pKa = 4.52 SPEE780 pKa = 4.09 KK781 pKa = 10.43 NGCPALVRR789 pKa = 11.84 KK790 pKa = 8.54 IVKK793 pKa = 9.23 MEE795 pKa = 3.7 EE796 pKa = 3.95 AHH798 pKa = 6.26 PGRR801 pKa = 11.84 YY802 pKa = 8.98 EE803 pKa = 3.65 YY804 pKa = 10.71 LGRR807 pKa = 11.84 SDD809 pKa = 5.23 PPLDD813 pKa = 4.95 DD814 pKa = 3.77 IRR816 pKa = 11.84 THH818 pKa = 6.57 EE819 pKa = 4.23 YY820 pKa = 10.11 SVSFQKK826 pKa = 10.38 YY827 pKa = 7.01 VCAISNIMLRR837 pKa = 11.84 TMWGEE842 pKa = 4.32 SIHH845 pKa = 6.31 HH846 pKa = 5.89 TMHH849 pKa = 6.55 MDD851 pKa = 3.17 ILNAFSSLTLDD862 pKa = 3.61 SVSTLKK868 pKa = 10.86 ASSAFDD874 pKa = 3.64 EE875 pKa = 4.2 MWYY878 pKa = 10.3 DD879 pKa = 3.29 SDD881 pKa = 5.01 GSVPYY886 pKa = 10.01 HH887 pKa = 6.18 RR888 pKa = 11.84 KK889 pKa = 9.64 KK890 pKa = 10.25 VVEE893 pKa = 4.03 NFRR896 pKa = 11.84 KK897 pKa = 10.02 FMTEE901 pKa = 3.68 GNTHH905 pKa = 4.33 VHH907 pKa = 5.9 HH908 pKa = 6.74 VLRR911 pKa = 11.84 EE912 pKa = 3.93 CLEE915 pKa = 4.35 TIEE918 pKa = 5.29 EE919 pKa = 4.35 RR920 pKa = 11.84 GCMHH924 pKa = 7.32 IDD926 pKa = 2.87 IFKK929 pKa = 10.25 KK930 pKa = 9.69 NQHH933 pKa = 5.46 GGLRR937 pKa = 11.84 EE938 pKa = 3.94 IYY940 pKa = 10.47 VLGPEE945 pKa = 4.07 EE946 pKa = 4.94 RR947 pKa = 11.84 VVQLALEE954 pKa = 4.71 TIARR958 pKa = 11.84 QVCKK962 pKa = 10.36 RR963 pKa = 11.84 FRR965 pKa = 11.84 SEE967 pKa = 3.87 TLTNPDD973 pKa = 3.26 QKK975 pKa = 10.01 TKK977 pKa = 10.38 IPEE980 pKa = 3.93 SHH982 pKa = 6.1 GRR984 pKa = 11.84 RR985 pKa = 11.84 ARR987 pKa = 11.84 AAQRR991 pKa = 11.84 PNDD994 pKa = 3.2 ITVNMEE1000 pKa = 4.15 TVGTSDD1006 pKa = 4.54 DD1007 pKa = 4.18 LKK1009 pKa = 10.43 TFNQTQHH1016 pKa = 4.39 TTKK1019 pKa = 10.74 LALTLIKK1026 pKa = 9.5 FTRR1029 pKa = 11.84 PSLHH1033 pKa = 6.45 PFIIRR1038 pKa = 11.84 ACSLFMRR1045 pKa = 11.84 KK1046 pKa = 9.18 RR1047 pKa = 11.84 IKK1049 pKa = 10.09 MDD1051 pKa = 4.63 DD1052 pKa = 4.17 DD1053 pKa = 3.89 LLQIIVKK1060 pKa = 10.57 NSDD1063 pKa = 3.65 LKK1065 pKa = 10.44 TDD1067 pKa = 3.96 DD1068 pKa = 3.78 PTLEE1072 pKa = 4.21 SLYY1075 pKa = 10.72 NAYY1078 pKa = 10.25 RR1079 pKa = 11.84 GNTVPCPRR1087 pKa = 11.84 WAEE1090 pKa = 3.95 EE1091 pKa = 3.82 GKK1093 pKa = 10.71 SYY1095 pKa = 11.03 IKK1097 pKa = 10.1 TEE1099 pKa = 3.66 TGMLQGILHH1108 pKa = 7.13 LLSSLHH1114 pKa = 6.05 HH1115 pKa = 5.25 TTIQVFYY1122 pKa = 10.27 RR1123 pKa = 11.84 YY1124 pKa = 9.35 YY1125 pKa = 10.84 AYY1127 pKa = 10.5 HH1128 pKa = 7.45 EE1129 pKa = 4.23 LTKK1132 pKa = 10.49 IHH1134 pKa = 7.41 DD1135 pKa = 3.72 GRR1137 pKa = 11.84 AGKK1140 pKa = 10.18 VLIDD1144 pKa = 3.83 IMQSSDD1150 pKa = 3.28 DD1151 pKa = 3.86 SAVLISYY1158 pKa = 7.75 PWTDD1162 pKa = 3.38 DD1163 pKa = 4.02 DD1164 pKa = 4.33 KK1165 pKa = 11.2 GKK1167 pKa = 10.64 KK1168 pKa = 8.92 SRR1170 pKa = 11.84 VMSAILLLLKK1180 pKa = 10.51 KK1181 pKa = 10.26 EE1182 pKa = 3.66 IGIFVGLYY1190 pKa = 9.93 PSVKK1194 pKa = 9.37 CTTNTLFFVEE1204 pKa = 5.07 FNSEE1208 pKa = 4.1 FFFHH1212 pKa = 7.04 NDD1214 pKa = 3.19 HH1215 pKa = 6.99 IRR1217 pKa = 11.84 PTVKK1221 pKa = 9.75 WVIACDD1227 pKa = 3.41 QVSEE1231 pKa = 4.27 QEE1233 pKa = 4.14 ALVARR1238 pKa = 11.84 QEE1240 pKa = 4.12 EE1241 pKa = 4.65 MASSLTGILEE1251 pKa = 4.57 GGGSISLCHH1260 pKa = 6.68 LCQVGQSILHH1270 pKa = 4.74 YY1271 pKa = 8.98 HH1272 pKa = 6.9 LLGASVSFLFSRR1284 pKa = 11.84 YY1285 pKa = 7.24 MNEE1288 pKa = 3.46 AKK1290 pKa = 10.4 KK1291 pKa = 10.81 FKK1293 pKa = 10.54 DD1294 pKa = 3.2 PSLGFFLLDD1303 pKa = 3.39 HH1304 pKa = 7.03 PFGAGITGFKK1314 pKa = 9.93 YY1315 pKa = 10.68 NLWNQVRR1322 pKa = 11.84 HH1323 pKa = 5.68 GSLGAIYY1330 pKa = 10.56 KK1331 pKa = 10.57 LFLTEE1336 pKa = 4.98 IKK1338 pKa = 10.59 ASTPPAVKK1346 pKa = 10.0 EE1347 pKa = 3.49 KK1348 pKa = 10.42 AYY1350 pKa = 10.7 RR1351 pKa = 11.84 SLEE1354 pKa = 4.18 TTTCGALASSVTVRR1368 pKa = 11.84 WGNRR1372 pKa = 11.84 KK1373 pKa = 8.67 KK1374 pKa = 9.69 WQALLDD1380 pKa = 4.02 RR1381 pKa = 11.84 LQVPSDD1387 pKa = 3.34 WEE1389 pKa = 4.24 EE1390 pKa = 4.04 KK1391 pKa = 10.01 QDD1393 pKa = 4.97 ANPEE1397 pKa = 3.85 CCYY1400 pKa = 10.17 RR1401 pKa = 11.84 HH1402 pKa = 6.04 PRR1404 pKa = 11.84 SAEE1407 pKa = 3.82 EE1408 pKa = 3.94 VLLKK1412 pKa = 10.74 VAEE1415 pKa = 4.28 KK1416 pKa = 10.26 LHH1418 pKa = 6.25 APGVSSSLTRR1428 pKa = 11.84 GDD1430 pKa = 4.49 HH1431 pKa = 5.61 ISKK1434 pKa = 10.04 IVSSSVYY1441 pKa = 8.45 ILSRR1445 pKa = 11.84 SVVGGGVSWMTPEE1458 pKa = 3.51 TDD1460 pKa = 3.37 TYY1462 pKa = 11.42 VKK1464 pKa = 10.64 EE1465 pKa = 3.88 PLLRR1469 pKa = 11.84 LLLKK1473 pKa = 10.9 QNDD1476 pKa = 3.38 IDD1478 pKa = 4.2 PNKK1481 pKa = 10.08 VMHH1484 pKa = 7.1 LDD1486 pKa = 3.74 DD1487 pKa = 4.61 EE1488 pKa = 4.7 EE1489 pKa = 4.43 VRR1491 pKa = 11.84 TLFPMDD1497 pKa = 3.65 SEE1499 pKa = 4.5 YY1500 pKa = 11.58 KK1501 pKa = 9.34 HH1502 pKa = 6.1 VKK1504 pKa = 10.0 NVLCSYY1510 pKa = 10.99 GSVEE1514 pKa = 3.96 GRR1516 pKa = 11.84 NQIGRR1521 pKa = 11.84 RR1522 pKa = 11.84 RR1523 pKa = 11.84 TVQTRR1528 pKa = 11.84 VIVFQRR1534 pKa = 11.84 EE1535 pKa = 3.38 EE1536 pKa = 3.73 MMRR1539 pKa = 11.84 AKK1541 pKa = 10.35 PEE1543 pKa = 4.12 DD1544 pKa = 3.9 VITDD1548 pKa = 3.39 KK1549 pKa = 9.93 WWAMGRR1555 pKa = 11.84 SGLTAIALKK1564 pKa = 10.83 DD1565 pKa = 3.48 HH1566 pKa = 7.01 FEE1568 pKa = 3.83 QLQRR1572 pKa = 11.84 VIPWLHH1578 pKa = 6.5 NDD1580 pKa = 3.3 PEE1582 pKa = 4.4 EE1583 pKa = 4.1 TLRR1586 pKa = 11.84 RR1587 pKa = 11.84 SPFLHH1592 pKa = 6.08 HH1593 pKa = 5.99 HH1594 pKa = 5.86 QIRR1597 pKa = 11.84 NFFSRR1602 pKa = 11.84 MDD1604 pKa = 3.28 YY1605 pKa = 10.58 HH1606 pKa = 8.17 GRR1608 pKa = 11.84 EE1609 pKa = 4.28 LKK1611 pKa = 10.87 LVGAPLRR1618 pKa = 11.84 QKK1620 pKa = 10.78 VEE1622 pKa = 4.12 TNIATTVARR1631 pKa = 11.84 NYY1633 pKa = 9.54 FPQWEE1638 pKa = 4.73 LSLQPDD1644 pKa = 3.07 VRR1646 pKa = 11.84 ARR1648 pKa = 11.84 DD1649 pKa = 3.23 RR1650 pKa = 11.84 ARR1652 pKa = 11.84 RR1653 pKa = 11.84 SDD1655 pKa = 3.36 AMKK1658 pKa = 10.26 HH1659 pKa = 4.29 FCYY1662 pKa = 10.54 LLTSGPYY1669 pKa = 10.4 DD1670 pKa = 3.29 SDD1672 pKa = 3.35 RR1673 pKa = 11.84 QTRR1676 pKa = 11.84 IFCDD1680 pKa = 3.12 VMRR1683 pKa = 11.84 YY1684 pKa = 9.91 VDD1686 pKa = 4.92 DD1687 pKa = 5.11 LDD1689 pKa = 4.03 VVAGAGKK1696 pKa = 8.19 TRR1698 pKa = 11.84 SNTLALMQKK1707 pKa = 8.97 YY1708 pKa = 9.84 VKK1710 pKa = 10.46 LDD1712 pKa = 3.49 NTQSSILNNEE1722 pKa = 3.52 IRR1724 pKa = 11.84 RR1725 pKa = 11.84 ANCGIVGGFHH1735 pKa = 7.4 RR1736 pKa = 11.84 PQQAKK1741 pKa = 10.13 EE1742 pKa = 3.55 IDD1744 pKa = 3.81 GKK1746 pKa = 10.63 VVYY1749 pKa = 9.84 QGPGTWIGTVHH1760 pKa = 5.65 GHH1762 pKa = 5.1 RR1763 pKa = 11.84 VEE1765 pKa = 4.46 IEE1767 pKa = 3.58 VDD1769 pKa = 3.55 SIGRR1773 pKa = 11.84 RR1774 pKa = 11.84 TEE1776 pKa = 3.78 VMAVRR1781 pKa = 11.84 VDD1783 pKa = 3.39 SSEE1786 pKa = 4.01 FARR1789 pKa = 11.84 MHH1791 pKa = 6.6 LVPFMKK1797 pKa = 10.11 QWGKK1801 pKa = 10.94 DD1802 pKa = 3.23 MNVCNTYY1809 pKa = 9.19 RR1810 pKa = 11.84 TVTAEE1815 pKa = 3.92 ASYY1818 pKa = 11.66 CMFNFEE1824 pKa = 4.42 IYY1826 pKa = 10.2 SYY1828 pKa = 10.56 GTGCPVFVHH1837 pKa = 6.08 EE1838 pKa = 5.16 RR1839 pKa = 11.84 LGVMPDD1845 pKa = 3.24 FQMGQMEE1852 pKa = 4.92 LLVNGHH1858 pKa = 7.1 CISVVCSAGSGRR1870 pKa = 11.84 RR1871 pKa = 11.84 VRR1873 pKa = 11.84 ILSFHH1878 pKa = 6.23 GRR1880 pKa = 11.84 PSDD1883 pKa = 3.71 VSKK1886 pKa = 11.15 PNADD1890 pKa = 3.43 QLEE1893 pKa = 4.31 DD1894 pKa = 4.71 EE1895 pKa = 4.86 IGQMLWAQKK1904 pKa = 10.02 EE1905 pKa = 4.28 PSHH1908 pKa = 6.88 SWFFLKK1914 pKa = 9.94 TMHH1917 pKa = 6.65 EE1918 pKa = 4.26 NTLAAHH1924 pKa = 7.26 LSHH1927 pKa = 7.27 IDD1929 pKa = 3.17 RR1930 pKa = 11.84 GKK1932 pKa = 10.2 ILPGTNTQVYY1942 pKa = 10.79 LDD1944 pKa = 3.99 ILRR1947 pKa = 11.84 DD1948 pKa = 3.46 ATGYY1952 pKa = 8.98 YY1953 pKa = 9.86 LRR1955 pKa = 11.84 SHH1957 pKa = 7.35 GYY1959 pKa = 8.19 TIHH1962 pKa = 7.37 DD1963 pKa = 4.08 MPRR1966 pKa = 11.84 ATISMGEE1973 pKa = 4.0 AEE1975 pKa = 3.95 AHH1977 pKa = 4.85 IRR1979 pKa = 11.84 QMEE1982 pKa = 4.11 EE1983 pKa = 3.07 ASAKK1987 pKa = 10.58 VMISDD1992 pKa = 3.74 VLVSVGLMTARR2003 pKa = 11.84 QDD2005 pKa = 3.65 EE2006 pKa = 4.54 AQQEE2010 pKa = 4.35 ADD2012 pKa = 3.94 GPPPSEE2018 pKa = 4.14 TTPTEE2023 pKa = 3.87 TGIIAAALEE2032 pKa = 4.28 EE2033 pKa = 4.26 TWAEE2037 pKa = 3.44 IMEE2040 pKa = 4.87 NIEE2043 pKa = 3.78 HH2044 pKa = 6.29 QARR2047 pKa = 11.84 GLQQDD2052 pKa = 3.47 EE2053 pKa = 4.83 MEE2055 pKa = 4.56 TDD2057 pKa = 3.48 DD2058 pKa = 4.03 VKK2060 pKa = 11.18 PRR2062 pKa = 11.84 TTPAIFLDD2070 pKa = 3.73 MPNLVVRR2077 pKa = 11.84 DD2078 pKa = 3.92 IFDD2081 pKa = 3.13 VDD2083 pKa = 3.67 AFGEE2087 pKa = 4.26 TDD2089 pKa = 3.86 FEE2091 pKa = 4.43 IMPRR2095 pKa = 11.84 SVYY2098 pKa = 10.38 NHH2100 pKa = 6.94 PSLVSRR2106 pKa = 11.84 YY2107 pKa = 8.34 IEE2109 pKa = 3.99 TKK2111 pKa = 7.2 ITHH2114 pKa = 7.27 IITPSEE2120 pKa = 3.91 MALLLTSNNILASKK2134 pKa = 10.34 SGAARR2139 pKa = 11.84 LLFRR2143 pKa = 11.84 LLQRR2147 pKa = 11.84 DD2148 pKa = 3.7 PEE2150 pKa = 4.75 RR2151 pKa = 11.84 INEE2154 pKa = 4.04 LTVPVTYY2161 pKa = 10.53 TEE2163 pKa = 4.6 DD2164 pKa = 4.03 LDD2166 pKa = 4.11 VASEE2170 pKa = 4.23 KK2171 pKa = 10.46 VDD2173 pKa = 3.08 EE2174 pKa = 4.64 SMFGG2178 pKa = 3.15
Molecular weight: 248.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.185
IPC2_protein 6.122
IPC_protein 6.198
Toseland 6.547
ProMoST 6.62
Dawson 6.547
Bjellqvist 6.547
Wikipedia 6.561
Rodwell 6.532
Grimsley 6.707
Solomon 6.547
Lehninger 6.547
Nozaki 6.839
DTASelect 7.0
Thurlkill 7.029
EMBOSS 7.0
Sillero 6.942
Patrickios 4.304
IPC_peptide 6.561
IPC2_peptide 6.825
IPC2.peptide.svr19 6.783
Protein with the highest isoelectric point:
>tr|A0A076E9Z2|A0A076E9Z2_9VIRU Nucleocapsid protein OS=American dog tick phlebovirus OX=1517960 PE=4 SV=1
MM1 pKa = 7.47 SKK3 pKa = 10.51 KK4 pKa = 9.63 PDD6 pKa = 3.5 QPSTSHH12 pKa = 7.04 RR13 pKa = 11.84 GRR15 pKa = 11.84 GSGRR19 pKa = 11.84 GTKK22 pKa = 9.47 KK23 pKa = 10.61 APPLAPKK30 pKa = 9.85 PKK32 pKa = 9.4 RR33 pKa = 11.84 ASIGDD38 pKa = 4.33 LISRR42 pKa = 11.84 SFSSTSSSEE51 pKa = 3.64 ADD53 pKa = 3.18 SPQEE57 pKa = 4.01 PEE59 pKa = 4.04 PTGQSAKK66 pKa = 10.04 EE67 pKa = 4.54 DD68 pKa = 4.09 PPTMPGRR75 pKa = 11.84 GGVGGANEE83 pKa = 4.53 TDD85 pKa = 3.3 PTAPGGGGAGGAGAGGANGGAGGGAGAGVVQPNAGDD121 pKa = 3.6 VMPEE125 pKa = 3.69 GTIIEE130 pKa = 4.23 LLGWALEE137 pKa = 4.39 RR138 pKa = 11.84 VTPEE142 pKa = 4.11 HH143 pKa = 7.08 KK144 pKa = 10.53 CLLTNAQLKK153 pKa = 10.3 RR154 pKa = 11.84 LLQAAVPYY162 pKa = 9.02 RR163 pKa = 11.84 TDD165 pKa = 2.84 EE166 pKa = 4.47 AYY168 pKa = 10.93 VLGVAQAVEE177 pKa = 4.2 YY178 pKa = 10.86 QGFDD182 pKa = 3.16 PAMVAKK188 pKa = 10.2 RR189 pKa = 11.84 VLEE192 pKa = 4.16 LAHH195 pKa = 6.39 QDD197 pKa = 3.12 RR198 pKa = 11.84 KK199 pKa = 10.02 EE200 pKa = 4.33 AEE202 pKa = 4.17 DD203 pKa = 4.01 VILDD207 pKa = 4.4 IITMVGLYY215 pKa = 7.63 LTRR218 pKa = 11.84 GTNLTKK224 pKa = 9.68 MEE226 pKa = 4.25 KK227 pKa = 9.94 RR228 pKa = 11.84 ISPEE232 pKa = 3.03 GLAILRR238 pKa = 11.84 RR239 pKa = 11.84 VSQRR243 pKa = 11.84 YY244 pKa = 7.76 NLKK247 pKa = 10.4 KK248 pKa = 10.56 GAVGPNEE255 pKa = 3.93 LTLSRR260 pKa = 11.84 VALTYY265 pKa = 10.91 ASITVRR271 pKa = 11.84 CAVQLKK277 pKa = 10.45 DD278 pKa = 3.71 RR279 pKa = 11.84 LAVPHH284 pKa = 7.48 DD285 pKa = 3.96 EE286 pKa = 4.7 MLDD289 pKa = 3.72 LSPGYY294 pKa = 9.46 PPALMTQAFASLIPKK309 pKa = 9.24 GHH311 pKa = 5.81 VEE313 pKa = 4.16 EE314 pKa = 4.89 EE315 pKa = 4.47 VLKK318 pKa = 10.64 HH319 pKa = 5.15 AHH321 pKa = 6.41 CLFLVKK327 pKa = 10.44 FSEE330 pKa = 4.62 VVNPQLRR337 pKa = 11.84 GKK339 pKa = 9.62 PGAEE343 pKa = 4.1 LKK345 pKa = 10.77 RR346 pKa = 11.84 SFTAALKK353 pKa = 10.64 ASLEE357 pKa = 4.14 KK358 pKa = 10.71 RR359 pKa = 11.84 EE360 pKa = 4.17 SRR362 pKa = 11.84 DD363 pKa = 3.37 NLRR366 pKa = 11.84 KK367 pKa = 9.31 IAFLKK372 pKa = 10.56 EE373 pKa = 3.81 MGVLSQEE380 pKa = 4.17 DD381 pKa = 3.97 TAVEE385 pKa = 4.26 AVRR388 pKa = 11.84 HH389 pKa = 5.13 AAAKK393 pKa = 7.48 FTNTYY398 pKa = 10.34 GPLEE402 pKa = 4.08
Molecular weight: 42.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.42
IPC2_protein 8.287
IPC_protein 8.229
Toseland 8.975
ProMoST 8.756
Dawson 9.238
Bjellqvist 9.048
Wikipedia 9.37
Rodwell 9.502
Grimsley 9.238
Solomon 9.341
Lehninger 9.311
Nozaki 9.224
DTASelect 8.96
Thurlkill 9.136
EMBOSS 9.428
Sillero 9.282
Patrickios 5.118
IPC_peptide 9.341
IPC2_peptide 7.629
IPC2.peptide.svr19 7.635
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
2580
402
2178
1290.0
145.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.55 ± 2.297
1.628 ± 0.414
5.891 ± 0.898
6.783 ± 0.202
3.76 ± 0.831
6.085 ± 2.165
3.372 ± 0.649
4.884 ± 0.892
6.085 ± 0.18
8.798 ± 0.541
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.333 ± 0.514
3.14 ± 0.306
5.078 ± 1.003
3.45 ± 0.015
6.124 ± 0.045
7.481 ± 0.592
6.24 ± 0.244
7.132 ± 0.195
1.279 ± 0.484
2.907 ± 0.431
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here