Cylindrobasidium torrendii FP15055 ss-10
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13901 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D7BB58|A0A0D7BB58_9AGAR Uncharacterized protein OS=Cylindrobasidium torrendii FP15055 ss-10 OX=1314674 GN=CYLTODRAFT_454512 PE=4 SV=1
MM1 pKa = 7.17 FALAALSLTVLPFVSADD18 pKa = 3.48 TALDD22 pKa = 3.6 LAGIKK27 pKa = 10.15 AHH29 pKa = 6.02 FTQSKK34 pKa = 9.54 IVPDD38 pKa = 4.1 LFEE41 pKa = 4.59 SFNPEE46 pKa = 3.03 AVLTVNFPGVGDD58 pKa = 3.72 ITPGQTLTQAEE69 pKa = 4.31 AGGYY73 pKa = 9.54 PSIKK77 pKa = 8.77 VTAANSSVDD86 pKa = 3.58 LGSTFTVAMVDD97 pKa = 3.33 ASYY100 pKa = 11.23 VGQDD104 pKa = 3.43 DD105 pKa = 5.55 DD106 pKa = 5.19 DD107 pKa = 4.31 VTRR110 pKa = 11.84 HH111 pKa = 5.15 WLMNGCTLSDD121 pKa = 3.48 GTISNSSATSITEE134 pKa = 3.96 YY135 pKa = 10.72 GGPAPPEE142 pKa = 4.11 GEE144 pKa = 3.76 PAHH147 pKa = 6.66 RR148 pKa = 11.84 YY149 pKa = 9.2 VIAVYY154 pKa = 8.16 QQPDD158 pKa = 3.63 SFSAPEE164 pKa = 4.44 GFNQANMGISTFDD177 pKa = 3.47 FEE179 pKa = 6.83 DD180 pKa = 3.91 YY181 pKa = 9.67 LTNSKK186 pKa = 10.54 LGALVGATYY195 pKa = 7.7 MTVQTGEE202 pKa = 3.86 ATTSVEE208 pKa = 4.1 ATSSVDD214 pKa = 3.49 SQTLSAASQTGSSSSGASATTGASGSSSSGAPDD247 pKa = 3.49 GASALTASSAAVALGFLGFLFAA269 pKa = 6.43
Molecular weight: 27.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.704
IPC2_protein 3.795
IPC_protein 3.783
Toseland 3.567
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.719
Rodwell 3.605
Grimsley 3.478
Solomon 3.77
Lehninger 3.719
Nozaki 3.897
DTASelect 4.126
Thurlkill 3.63
EMBOSS 3.732
Sillero 3.91
Patrickios 0.998
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.801
Protein with the highest isoelectric point:
>tr|A0A0D7B3P3|A0A0D7B3P3_9AGAR Ribosomal protein OS=Cylindrobasidium torrendii FP15055 ss-10 OX=1314674 GN=CYLTODRAFT_456859 PE=3 SV=1
CC1 pKa = 7.47 LLAKK5 pKa = 10.53 LLLRR9 pKa = 11.84 TPTSPRR15 pKa = 11.84 VVLRR19 pKa = 11.84 ASRR22 pKa = 11.84 SSRR25 pKa = 11.84 SQLKK29 pKa = 10.05 SPRR32 pKa = 11.84 RR33 pKa = 11.84 PPRR36 pKa = 11.84 NPAPKK41 pKa = 10.16 KK42 pKa = 9.71 PIRR45 pKa = 11.84 LRR47 pKa = 11.84 TARR50 pKa = 11.84 RR51 pKa = 11.84 KK52 pKa = 9.25 PRR54 pKa = 11.84 NLRR57 pKa = 11.84 ARR59 pKa = 11.84 SARR62 pKa = 11.84 LLKK65 pKa = 9.92 TLTKK69 pKa = 9.7 PPSPRR74 pKa = 11.84 RR75 pKa = 11.84 PNPPAKK81 pKa = 9.98 RR82 pKa = 11.84 SLLPRR87 pKa = 11.84 QSPPARR93 pKa = 11.84 LSQRR97 pKa = 11.84 PRR99 pKa = 11.84 QHH101 pKa = 4.75 QHH103 pKa = 6.22 RR104 pKa = 11.84 SLHH107 pKa = 5.82 PALLRR112 pKa = 11.84 PSQRR116 pKa = 11.84 LVLLLSQRR124 pKa = 11.84 LGLRR128 pKa = 11.84 QQSLPLVPPPRR139 pKa = 11.84 NLHH142 pKa = 5.96 RR143 pKa = 11.84 ALGPRR148 pKa = 11.84 SRR150 pKa = 11.84 HH151 pKa = 5.15 PRR153 pKa = 11.84 LVRR156 pKa = 11.84 PPLPLLRR163 pKa = 11.84 PPPRR167 pKa = 11.84 RR168 pKa = 11.84 QSLKK172 pKa = 10.59 SRR174 pKa = 11.84 NPLKK178 pKa = 9.71 KK179 pKa = 8.88 TKK181 pKa = 9.98 LRR183 pKa = 11.84 PPLKK187 pKa = 8.14 TRR189 pKa = 11.84 PRR191 pKa = 11.84 RR192 pKa = 11.84 RR193 pKa = 11.84 CPRR196 pKa = 11.84 RR197 pKa = 11.84 RR198 pKa = 11.84 KK199 pKa = 9.56 PEE201 pKa = 3.76 NGRR204 pKa = 11.84 GWWAIGYY211 pKa = 9.39 RR212 pKa = 11.84 LL213 pKa = 3.88
Molecular weight: 24.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.515
IPC2_protein 11.242
IPC_protein 12.778
Toseland 12.939
ProMoST 13.437
Dawson 12.939
Bjellqvist 12.939
Wikipedia 13.422
Rodwell 12.559
Grimsley 12.983
Solomon 13.437
Lehninger 13.334
Nozaki 12.939
DTASelect 12.939
Thurlkill 12.939
EMBOSS 13.437
Sillero 12.939
Patrickios 12.266
IPC_peptide 13.437
IPC2_peptide 12.427
IPC2.peptide.svr19 9.174
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13892
9
13901
5747761
50
4803
413.5
45.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.91 ± 0.019
1.314 ± 0.009
5.796 ± 0.016
5.907 ± 0.02
3.737 ± 0.013
6.414 ± 0.021
2.523 ± 0.008
4.729 ± 0.014
4.558 ± 0.019
9.116 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.234 ± 0.008
3.378 ± 0.011
6.436 ± 0.03
3.644 ± 0.013
6.276 ± 0.018
8.307 ± 0.026
6.073 ± 0.012
6.398 ± 0.014
1.509 ± 0.009
2.741 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here