Salmon gill poxvirus
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 206 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0H4Y1D3|A0A0H4Y1D3_9POXV Uncharacterized protein OS=Salmon gill poxvirus OX=1680908 GN=SGPV181 PE=4 SV=1
MM1 pKa = 7.58 SNHH4 pKa = 7.08 LYY6 pKa = 10.74 LFPDD10 pKa = 3.47 YY11 pKa = 10.86 TLVDD15 pKa = 4.53 DD16 pKa = 4.35 NTNPLVDD23 pKa = 3.92 VNIVTFDD30 pKa = 3.23 KK31 pKa = 10.68 EE32 pKa = 4.27 AYY34 pKa = 9.28 SNKK37 pKa = 10.02 QIVLPDD43 pKa = 3.22 INFIPDD49 pKa = 3.61 IMPIEE54 pKa = 4.29 EE55 pKa = 4.28 SVKK58 pKa = 10.3 FIPLEE63 pKa = 4.33 TVFSRR68 pKa = 11.84 QYY70 pKa = 10.79 NDD72 pKa = 4.55 FISEE76 pKa = 4.27 EE77 pKa = 4.15 IEE79 pKa = 4.39 SDD81 pKa = 3.44 EE82 pKa = 5.28 LDD84 pKa = 3.59 YY85 pKa = 11.63 DD86 pKa = 4.02 SDD88 pKa = 4.08 SVQYY92 pKa = 8.28 EE93 pKa = 4.13 TGEE96 pKa = 3.77 YY97 pKa = 10.12 DD98 pKa = 3.75 YY99 pKa = 10.92 EE100 pKa = 4.23 NTVNSVGLHH109 pKa = 6.57 DD110 pKa = 4.92 DD111 pKa = 2.71 MWMEE115 pKa = 4.4 SDD117 pKa = 4.54 HH118 pKa = 7.73 DD119 pKa = 4.12 SDD121 pKa = 5.21 YY122 pKa = 11.79 EE123 pKa = 4.06 NDD125 pKa = 4.26 CEE127 pKa = 5.45 DD128 pKa = 3.74 SYY130 pKa = 11.8 ILQSHH135 pKa = 7.65 FIHH138 pKa = 7.33
Molecular weight: 16.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.834
IPC2_protein 3.617
IPC_protein 3.617
Toseland 3.401
ProMoST 3.783
Dawson 3.617
Bjellqvist 3.77
Wikipedia 3.554
Rodwell 3.452
Grimsley 3.312
Solomon 3.605
Lehninger 3.554
Nozaki 3.732
DTASelect 3.961
Thurlkill 3.465
EMBOSS 3.567
Sillero 3.745
Patrickios 0.223
IPC_peptide 3.592
IPC2_peptide 3.719
IPC2.peptide.svr19 3.686
Protein with the highest isoelectric point:
>tr|A0A0H4XWP4|A0A0H4XWP4_9POXV G1L metaloprotease-like protein OS=Salmon gill poxvirus OX=1680908 GN=SGPV149 PE=3 SV=1
MM1 pKa = 7.71 SNRR4 pKa = 11.84 KK5 pKa = 8.76 HH6 pKa = 6.4 DD7 pKa = 4.08 RR8 pKa = 11.84 PRR10 pKa = 11.84 VITSWTSCVIIKK22 pKa = 9.83 IRR24 pKa = 11.84 YY25 pKa = 7.0 QYY27 pKa = 10.53 TYY29 pKa = 9.33 GLVRR33 pKa = 11.84 IVRR36 pKa = 11.84 LFCSGLVRR44 pKa = 11.84 IVRR47 pKa = 11.84 IVRR50 pKa = 11.84 NLSSRR55 pKa = 11.84 NLSSRR60 pKa = 11.84 NLSSRR65 pKa = 11.84 NLSSRR70 pKa = 11.84 NLSSRR75 pKa = 11.84 ILSRR79 pKa = 11.84 ILSRR83 pKa = 11.84 NLSSRR88 pKa = 11.84 NLSSRR93 pKa = 11.84 NLSSRR98 pKa = 11.84 NLSSRR103 pKa = 11.84 NLSSXKK109 pKa = 9.9 PQQ111 pKa = 3.24
Molecular weight: 12.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.254
IPC2_protein 10.774
IPC_protein 12.12
Toseland 12.266
ProMoST 12.764
Dawson 12.281
Bjellqvist 12.266
Wikipedia 12.749
Rodwell 11.857
Grimsley 12.31
Solomon 12.764
Lehninger 12.676
Nozaki 12.266
DTASelect 12.266
Thurlkill 12.266
EMBOSS 12.778
Sillero 12.281
Patrickios 11.579
IPC_peptide 12.778
IPC2_peptide 11.769
IPC2.peptide.svr19 9.17
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
206
0
206
75061
53
2493
364.4
41.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.896 ± 0.135
1.921 ± 0.095
6.539 ± 0.134
5.705 ± 0.128
4.925 ± 0.112
4.584 ± 0.141
2.417 ± 0.089
7.702 ± 0.181
7.992 ± 0.175
8.5 ± 0.136
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.026 ± 0.072
6.499 ± 0.172
4.109 ± 0.131
2.946 ± 0.086
3.514 ± 0.126
7.57 ± 0.144
7.181 ± 0.133
6.503 ± 0.116
1.298 ± 0.064
4.169 ± 0.096
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here