Influenza A virus (A/mallard/Wisconsin/08OS2271/2008(H11N9))
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E8Z093|E8Z093_9INFA Non-structural protein 1 OS=Influenza A virus (A/mallard/Wisconsin/08OS2271/2008(H11N9)) OX=878462 GN=NS1 PE=3 SV=1
MM1 pKa = 8.19 DD2 pKa = 5.4 SNTVSSFQDD11 pKa = 2.71 ILMRR15 pKa = 11.84 MSKK18 pKa = 8.28 MQLGSSSEE26 pKa = 4.23 DD27 pKa = 3.28 LNGMITQFEE36 pKa = 4.44 SLKK39 pKa = 10.58 LYY41 pKa = 10.39 RR42 pKa = 11.84 DD43 pKa = 3.79 SLGEE47 pKa = 3.64 AVMRR51 pKa = 11.84 MGDD54 pKa = 3.59 LHH56 pKa = 7.5 SLQSRR61 pKa = 11.84 NGKK64 pKa = 8.04 WRR66 pKa = 11.84 EE67 pKa = 3.59 QLSQKK72 pKa = 10.19 FEE74 pKa = 4.44 EE75 pKa = 4.25 IRR77 pKa = 11.84 WLIEE81 pKa = 3.91 EE82 pKa = 3.9 VRR84 pKa = 11.84 HH85 pKa = 5.48 RR86 pKa = 11.84 LKK88 pKa = 9.97 VTEE91 pKa = 4.3 NSFEE95 pKa = 4.33 QITFMQALQLLLEE108 pKa = 4.42 VEE110 pKa = 4.13 QEE112 pKa = 3.38 IRR114 pKa = 11.84 TFSFQLII121 pKa = 3.9
Molecular weight: 14.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.247
IPC2_protein 5.258
IPC_protein 5.105
Toseland 5.054
ProMoST 5.219
Dawson 5.067
Bjellqvist 5.207
Wikipedia 4.914
Rodwell 5.003
Grimsley 4.978
Solomon 5.067
Lehninger 5.029
Nozaki 5.194
DTASelect 5.283
Thurlkill 5.041
EMBOSS 4.952
Sillero 5.27
Patrickios 4.647
IPC_peptide 5.08
IPC2_peptide 5.27
IPC2.peptide.svr19 5.312
Protein with the highest isoelectric point:
>tr|E8Z092|E8Z092_9INFA Nuclear export protein OS=Influenza A virus (A/mallard/Wisconsin/08OS2271/2008(H11N9)) OX=878462 GN=NEP PE=3 SV=1
MM1 pKa = 7.78 ASQGTKK7 pKa = 9.71 RR8 pKa = 11.84 SYY10 pKa = 10.97 EE11 pKa = 3.79 QMEE14 pKa = 4.48 TSGEE18 pKa = 4.06 RR19 pKa = 11.84 QNATEE24 pKa = 3.76 IRR26 pKa = 11.84 ASVGRR31 pKa = 11.84 MVGGIGRR38 pKa = 11.84 FYY40 pKa = 10.72 IQMCTEE46 pKa = 4.87 LKK48 pKa = 10.71 LSDD51 pKa = 3.6 YY52 pKa = 10.66 EE53 pKa = 4.36 GRR55 pKa = 11.84 LIQNSITIEE64 pKa = 4.06 RR65 pKa = 11.84 MVLSAFDD72 pKa = 3.57 EE73 pKa = 4.31 RR74 pKa = 11.84 RR75 pKa = 11.84 NKK77 pKa = 10.12 YY78 pKa = 10.45 LEE80 pKa = 3.98 EE81 pKa = 4.54 HH82 pKa = 6.8 PSAGKK87 pKa = 10.07 DD88 pKa = 3.33 PKK90 pKa = 10.14 KK91 pKa = 9.42 TGGPIYY97 pKa = 10.21 RR98 pKa = 11.84 RR99 pKa = 11.84 RR100 pKa = 11.84 DD101 pKa = 3.62 GKK103 pKa = 8.92 WVRR106 pKa = 11.84 EE107 pKa = 4.0 LILYY111 pKa = 10.04 DD112 pKa = 3.4 KK113 pKa = 11.39 EE114 pKa = 3.93 EE115 pKa = 3.69 IRR117 pKa = 11.84 RR118 pKa = 11.84 IWRR121 pKa = 11.84 QANNGEE127 pKa = 4.33 DD128 pKa = 3.23 ATAGLTHH135 pKa = 6.13 MMIWHH140 pKa = 6.62 SNLNDD145 pKa = 3.0 ATYY148 pKa = 10.71 QRR150 pKa = 11.84 TRR152 pKa = 11.84 ALVRR156 pKa = 11.84 TGMDD160 pKa = 2.98 PRR162 pKa = 11.84 MCSLMQGSTLPRR174 pKa = 11.84 RR175 pKa = 11.84 SGAAGAAVKK184 pKa = 10.56 GVGTMVMEE192 pKa = 5.61 LIRR195 pKa = 11.84 MIKK198 pKa = 10.19 RR199 pKa = 11.84 GINDD203 pKa = 3.34 RR204 pKa = 11.84 NFWRR208 pKa = 11.84 GEE210 pKa = 3.54 NGRR213 pKa = 11.84 RR214 pKa = 11.84 TRR216 pKa = 11.84 IAYY219 pKa = 9.45 EE220 pKa = 3.95 RR221 pKa = 11.84 MCNILKK227 pKa = 10.75 GKK229 pKa = 9.34 FQTAAQRR236 pKa = 11.84 AMMDD240 pKa = 3.11 QVRR243 pKa = 11.84 EE244 pKa = 4.04 SRR246 pKa = 11.84 NPGNAEE252 pKa = 3.62 IEE254 pKa = 4.16 DD255 pKa = 4.8 LIFLARR261 pKa = 11.84 SALILRR267 pKa = 11.84 GSVAHH272 pKa = 6.78 KK273 pKa = 10.44 SCLPACVYY281 pKa = 10.62 GLAVASGYY289 pKa = 10.7 DD290 pKa = 3.72 FEE292 pKa = 6.06 RR293 pKa = 11.84 EE294 pKa = 4.56 GYY296 pKa = 9.53 SLVGIDD302 pKa = 5.28 PFRR305 pKa = 11.84 LLQNSQVFSLIRR317 pKa = 11.84 PNEE320 pKa = 4.06 NPAHH324 pKa = 6.86 KK325 pKa = 10.49 SQLVWMACHH334 pKa = 6.25 SAAFEE339 pKa = 4.24 DD340 pKa = 4.17 LRR342 pKa = 11.84 VSSFIRR348 pKa = 11.84 GTRR351 pKa = 11.84 VVPRR355 pKa = 11.84 GQLSTRR361 pKa = 11.84 GVQIASNEE369 pKa = 3.94 NMEE372 pKa = 4.48 TMDD375 pKa = 4.72 SSTLEE380 pKa = 3.59 LRR382 pKa = 11.84 SRR384 pKa = 11.84 YY385 pKa = 7.55 WAIRR389 pKa = 11.84 TRR391 pKa = 11.84 SGGNTNQQRR400 pKa = 11.84 ASAGQISVQPTFSVQRR416 pKa = 11.84 NLPFEE421 pKa = 4.38 RR422 pKa = 11.84 ATIMAAFTGNTEE434 pKa = 3.86 GRR436 pKa = 11.84 TSDD439 pKa = 3.19 MRR441 pKa = 11.84 TEE443 pKa = 4.9 IIRR446 pKa = 11.84 MMEE449 pKa = 3.73 NARR452 pKa = 11.84 PEE454 pKa = 4.17 DD455 pKa = 3.45 VSFQGRR461 pKa = 11.84 GVFEE465 pKa = 4.95 LSDD468 pKa = 3.67 EE469 pKa = 4.35 KK470 pKa = 10.77 ATNPIVPSFDD480 pKa = 3.22 MSNEE484 pKa = 3.24 GSYY487 pKa = 11.04 FFGDD491 pKa = 3.16 NAEE494 pKa = 4.48 EE495 pKa = 4.16 YY496 pKa = 11.1 DD497 pKa = 3.62 NN498 pKa = 4.9
Molecular weight: 56.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.887
IPC2_protein 8.77
IPC_protein 8.843
Toseland 9.253
ProMoST 9.194
Dawson 9.604
Bjellqvist 9.458
Wikipedia 9.823
Rodwell 9.692
Grimsley 9.692
Solomon 9.663
Lehninger 9.604
Nozaki 9.502
DTASelect 9.385
Thurlkill 9.443
EMBOSS 9.721
Sillero 9.604
Patrickios 5.003
IPC_peptide 9.648
IPC2_peptide 8.346
IPC2.peptide.svr19 7.763
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2035
121
716
407.0
46.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.848 ± 0.647
2.26 ± 0.656
4.816 ± 0.419
8.452 ± 1.126
3.882 ± 0.576
6.437 ± 0.707
1.523 ± 0.173
6.634 ± 0.341
4.963 ± 1.006
7.715 ± 0.972
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.538 ± 0.706
5.455 ± 0.94
4.079 ± 0.549
3.735 ± 0.664
7.42 ± 1.252
7.813 ± 0.465
5.946 ± 0.508
4.914 ± 0.285
1.867 ± 0.321
2.703 ± 0.537
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here