bacterium endosymbiont of Pedicinus badii

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 8.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 529 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V8NCW4|A0A1V8NCW4_9BACT UDP-N-acetylglucosamine 1-carboxyvinyltransferase OS=bacterium endosymbiont of Pedicinus badii OX=1719126 GN=murA PE=3 SV=1
MM1 pKa = 7.41NSIEE5 pKa = 4.5KK6 pKa = 9.76KK7 pKa = 7.69VKK9 pKa = 10.35KK10 pKa = 10.37IIVEE14 pKa = 3.74QLGVKK19 pKa = 10.05KK20 pKa = 10.81EE21 pKa = 4.16EE22 pKa = 4.32VVNTASFVEE31 pKa = 4.26DD32 pKa = 4.48LGADD36 pKa = 3.39SLDD39 pKa = 3.64TVEE42 pKa = 5.9LVMALEE48 pKa = 4.32EE49 pKa = 4.24EE50 pKa = 4.31FDD52 pKa = 3.79IEE54 pKa = 4.81IPDD57 pKa = 3.67EE58 pKa = 4.11EE59 pKa = 4.53AEE61 pKa = 5.03KK62 pKa = 9.69ITTVQAAIDD71 pKa = 4.15FIKK74 pKa = 10.65KK75 pKa = 9.35NKK77 pKa = 9.01KK78 pKa = 9.38

Molecular weight: 8.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V8NCL3|A0A1V8NCL3_9BACT Uncharacterized protein OS=bacterium endosymbiont of Pedicinus badii OX=1719126 GN=AOQ89_01435 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.21QPSVLKK11 pKa = 10.67KK12 pKa = 9.43NRR14 pKa = 11.84KK15 pKa = 8.27HH16 pKa = 5.79GFRR19 pKa = 11.84SRR21 pKa = 11.84ISTKK25 pKa = 9.85NGRR28 pKa = 11.84IILARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84MKK37 pKa = 10.8NRR39 pKa = 11.84IHH41 pKa = 6.09LTVSSKK47 pKa = 11.21

Molecular weight: 5.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

529

0

529

164887

38

1414

311.7

36.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.383 ± 0.09

1.289 ± 0.03

3.243 ± 0.058

5.785 ± 0.103

6.814 ± 0.143

4.937 ± 0.097

1.43 ± 0.035

12.892 ± 0.108

14.184 ± 0.17

8.467 ± 0.09

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.876 ± 0.038

6.775 ± 0.081

2.555 ± 0.051

2.661 ± 0.051

3.402 ± 0.062

7.298 ± 0.079

3.789 ± 0.053

4.475 ± 0.075

0.753 ± 0.029

3.991 ± 0.07
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski