Pseudomonas phage vB_Pae_PS44

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Pbunavirus; Pseudomonas virus PS44

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K0L952|A0A0K0L952_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_Pae_PS44 OX=1542090 GN=vB_Pae_PS44_00087 PE=4 SV=1
MM1 pKa = 7.53SGHH4 pKa = 6.25SLFDD8 pKa = 3.96FEE10 pKa = 5.22VDD12 pKa = 3.95CNSATNSATMEE23 pKa = 4.04IDD25 pKa = 3.14PGSFYY30 pKa = 11.36VGFGFGATALSVLIYY45 pKa = 10.73VDD47 pKa = 4.27GVWVTEE53 pKa = 4.03RR54 pKa = 11.84TPWIPDD60 pKa = 3.37PEE62 pKa = 4.71EE63 pKa = 4.03ISSS66 pKa = 3.54

Molecular weight: 7.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K0L944|A0A0K0L944_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_Pae_PS44 OX=1542090 GN=vB_Pae_PS44_00077 PE=4 SV=1
MM1 pKa = 7.73AEE3 pKa = 4.6HH4 pKa = 7.28IFTHH8 pKa = 6.53EE9 pKa = 4.01GTVVFEE15 pKa = 4.13GRR17 pKa = 11.84SRR19 pKa = 11.84TVKK22 pKa = 10.03LRR24 pKa = 11.84RR25 pKa = 11.84GNHH28 pKa = 5.13HH29 pKa = 6.3WVDD32 pKa = 4.7FDD34 pKa = 3.41RR35 pKa = 11.84HH36 pKa = 6.08RR37 pKa = 11.84YY38 pKa = 8.76SPQDD42 pKa = 3.0GRR44 pKa = 11.84AASQAIKK51 pKa = 10.58GAVLVLSTVRR61 pKa = 11.84WLPGAKK67 pKa = 9.2EE68 pKa = 3.62AAEE71 pKa = 3.87QAKK74 pKa = 10.1AFPYY78 pKa = 9.72KK79 pKa = 10.71GRR81 pKa = 11.84VHH83 pKa = 6.94ALGAAWHH90 pKa = 5.64YY91 pKa = 10.96VLLRR95 pKa = 11.84RR96 pKa = 11.84TKK98 pKa = 10.53NFYY101 pKa = 10.81VDD103 pKa = 3.42PQGRR107 pKa = 11.84KK108 pKa = 6.94YY109 pKa = 10.56RR110 pKa = 11.84RR111 pKa = 11.84NTGWSVDD118 pKa = 3.56GLLKK122 pKa = 10.91LSLDD126 pKa = 3.96SIKK129 pKa = 10.74PSVGEE134 pKa = 3.88RR135 pKa = 11.84KK136 pKa = 9.66YY137 pKa = 11.69VSII140 pKa = 5.08

Molecular weight: 15.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

20155

31

1035

214.4

23.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.933 ± 0.351

0.923 ± 0.088

6.261 ± 0.188

7.065 ± 0.41

4.158 ± 0.192

7.229 ± 0.247

1.737 ± 0.155

6.108 ± 0.198

5.354 ± 0.318

7.422 ± 0.223

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.545 ± 0.113

4.257 ± 0.294

4.53 ± 0.232

3.84 ± 0.168

6.222 ± 0.256

6.321 ± 0.199

5.096 ± 0.24

6.544 ± 0.229

1.583 ± 0.113

2.858 ± 0.116
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski