Beihai sobemo-like virus 4

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KE91|A0A1L3KE91_9VIRU Uncharacterized protein OS=Beihai sobemo-like virus 4 OX=1922701 PE=4 SV=1
MM1 pKa = 7.64RR2 pKa = 11.84LEE4 pKa = 4.15PSGGSGFDD12 pKa = 2.93EE13 pKa = 4.35DD14 pKa = 5.94FRR16 pKa = 11.84VNWRR20 pKa = 11.84WSEE23 pKa = 3.92PQPTTCGEE31 pKa = 4.12SALPDD36 pKa = 3.4ARR38 pKa = 11.84VSRR41 pKa = 11.84SLDD44 pKa = 3.09QTLWKK49 pKa = 10.68GVFRR53 pKa = 11.84VNTSSGVYY61 pKa = 10.48DD62 pKa = 4.02SMGLNIAGCALLCGHH77 pKa = 6.2SVRR80 pKa = 11.84GKK82 pKa = 10.63DD83 pKa = 3.72FVVLSGGNSKK93 pKa = 10.71AAGVKK98 pKa = 9.4IAVSRR103 pKa = 11.84FSIPLSYY110 pKa = 11.17VEE112 pKa = 5.92GGATDD117 pKa = 3.93FAVARR122 pKa = 11.84LTGNEE127 pKa = 3.51WAAIGAKK134 pKa = 10.06ALSSKK139 pKa = 10.91DD140 pKa = 3.43FASPNAGSIDD150 pKa = 3.3IQYY153 pKa = 10.04GQGSEE158 pKa = 4.59GILFVSEE165 pKa = 3.98GRR167 pKa = 11.84LPQQAKK173 pKa = 9.06AVEE176 pKa = 3.95RR177 pKa = 11.84AGLVFHH183 pKa = 6.36QVSTQPGSSGGALRR197 pKa = 11.84RR198 pKa = 11.84FVGGVPKK205 pKa = 10.85YY206 pKa = 10.89VGFHH210 pKa = 4.78VARR213 pKa = 11.84PQGKK217 pKa = 8.51FVDD220 pKa = 3.94LTGKK224 pKa = 10.67YY225 pKa = 9.32NVAVSFDD232 pKa = 3.88IIFSYY237 pKa = 9.45MRR239 pKa = 11.84SVGLYY244 pKa = 8.25YY245 pKa = 10.7DD246 pKa = 3.74SVFAAVMKK254 pKa = 10.5AAVGEE259 pKa = 4.22SLDD262 pKa = 3.71YY263 pKa = 11.67DD264 pKa = 3.83NDD266 pKa = 3.67NVRR269 pKa = 11.84DD270 pKa = 3.75PLDD273 pKa = 3.33FHH275 pKa = 7.31RR276 pKa = 11.84VSQEE280 pKa = 3.8CYY282 pKa = 10.13EE283 pKa = 5.23DD284 pKa = 4.55DD285 pKa = 4.53EE286 pKa = 4.66LWEE289 pKa = 4.26AAQDD293 pKa = 3.9RR294 pKa = 11.84IISEE298 pKa = 4.15IYY300 pKa = 9.75GDD302 pKa = 3.44RR303 pKa = 11.84GQAYY307 pKa = 8.38EE308 pKa = 3.86GHH310 pKa = 6.28IGRR313 pKa = 11.84RR314 pKa = 11.84FRR316 pKa = 11.84RR317 pKa = 11.84RR318 pKa = 11.84GDD320 pKa = 3.54DD321 pKa = 3.61EE322 pKa = 4.43PVGEE326 pKa = 4.36SLPVLVEE333 pKa = 3.99NDD335 pKa = 3.6DD336 pKa = 3.87EE337 pKa = 5.02YY338 pKa = 11.59EE339 pKa = 4.17DD340 pKa = 4.29VEE342 pKa = 4.35EE343 pKa = 4.71ALADD347 pKa = 3.81SSTVSHH353 pKa = 7.54DD354 pKa = 3.82GPPAQPSLLPPKK366 pKa = 10.48SIGEE370 pKa = 4.27SAISLSTVAEE380 pKa = 4.28EE381 pKa = 4.25TADD384 pKa = 3.55AAAVCLPLALDD395 pKa = 3.89LADD398 pKa = 3.99VKK400 pKa = 11.03KK401 pKa = 10.61QVINGDD407 pKa = 3.6FSFAPAVRR415 pKa = 11.84SAVEE419 pKa = 3.63LHH421 pKa = 6.3GVDD424 pKa = 4.71AIHH427 pKa = 6.49EE428 pKa = 4.44FVMSSDD434 pKa = 3.11AFSVYY439 pKa = 9.67RR440 pKa = 11.84SYY442 pKa = 11.3MDD444 pKa = 3.27VVQPTWFPFGEE455 pKa = 4.3VMPDD459 pKa = 3.16EE460 pKa = 4.37NGKK463 pKa = 10.17AFFEE467 pKa = 4.49KK468 pKa = 10.14VGEE471 pKa = 4.0YY472 pKa = 10.14RR473 pKa = 11.84VDD475 pKa = 3.55GVKK478 pKa = 10.35SATTPDD484 pKa = 3.31RR485 pKa = 11.84KK486 pKa = 10.33KK487 pKa = 10.59KK488 pKa = 10.45SKK490 pKa = 9.99PLSDD494 pKa = 3.52VAKK497 pKa = 10.07EE498 pKa = 3.27RR499 pKa = 11.84AAAIKK504 pKa = 10.55ALLKK508 pKa = 10.93DD509 pKa = 4.5LGCEE513 pKa = 3.78DD514 pKa = 5.13DD515 pKa = 3.89EE516 pKa = 4.74WVTPEE521 pKa = 3.96NSRR524 pKa = 11.84ANISASMRR532 pKa = 11.84AHH534 pKa = 7.03AKK536 pKa = 9.62LASVDD541 pKa = 3.77PPAASAADD549 pKa = 3.47WEE551 pKa = 4.56RR552 pKa = 11.84ALEE555 pKa = 4.24AGVADD560 pKa = 5.28FDD562 pKa = 4.24TTVLKK567 pKa = 10.8SHH569 pKa = 6.72AQQGFEE575 pKa = 3.76GWYY578 pKa = 10.45KK579 pKa = 10.65LAATLADD586 pKa = 3.56TSSGVSARR594 pKa = 11.84FRR596 pKa = 11.84RR597 pKa = 11.84QNKK600 pKa = 8.46RR601 pKa = 11.84QWATDD606 pKa = 3.78PEE608 pKa = 4.62LLLAMIDD615 pKa = 3.83LVQCRR620 pKa = 11.84LVLMLIHH627 pKa = 7.12ADD629 pKa = 3.46SVSGYY634 pKa = 8.19TPEE637 pKa = 3.98QAVKK641 pKa = 10.78YY642 pKa = 9.55GLKK645 pKa = 10.41DD646 pKa = 3.51VLLLSVKK653 pKa = 9.95QEE655 pKa = 3.47PHH657 pKa = 5.93APKK660 pKa = 9.98KK661 pKa = 10.39AKK663 pKa = 9.21QGRR666 pKa = 11.84YY667 pKa = 8.17RR668 pKa = 11.84MIWISSLIDD677 pKa = 3.46SFVQKK682 pKa = 10.55LLHH685 pKa = 6.34KK686 pKa = 10.25ALNARR691 pKa = 11.84DD692 pKa = 3.44IDD694 pKa = 4.2HH695 pKa = 6.06YY696 pKa = 11.35QSGEE700 pKa = 4.17RR701 pKa = 11.84YY702 pKa = 9.34HH703 pKa = 6.63SAAGMGHH710 pKa = 6.46HH711 pKa = 7.67DD712 pKa = 3.91EE713 pKa = 5.32GIKK716 pKa = 10.25HH717 pKa = 6.28LCSAFDD723 pKa = 3.45AVFGDD728 pKa = 4.01EE729 pKa = 5.3EE730 pKa = 4.49FLLTCDD736 pKa = 3.15ASMWDD741 pKa = 3.52FTMDD745 pKa = 3.18KK746 pKa = 10.19QAHH749 pKa = 6.06LNHH752 pKa = 6.81AKK754 pKa = 9.57RR755 pKa = 11.84RR756 pKa = 11.84CMSCDD761 pKa = 3.42DD762 pKa = 4.43PAVSSLIMTLAHH774 pKa = 6.95LNYY777 pKa = 10.52KK778 pKa = 9.29HH779 pKa = 6.23VCEE782 pKa = 4.41NKK784 pKa = 10.29GDD786 pKa = 3.41VWRR789 pKa = 11.84CNKK792 pKa = 10.27EE793 pKa = 3.93GVNTSGQSSTTADD806 pKa = 3.24NTFTRR811 pKa = 11.84HH812 pKa = 4.82SQAKK816 pKa = 8.52VCGARR821 pKa = 11.84KK822 pKa = 9.0FVGNGDD828 pKa = 4.0DD829 pKa = 3.43MVADD833 pKa = 3.58VGFDD837 pKa = 3.33PEE839 pKa = 4.08AAKK842 pKa = 10.75KK843 pKa = 10.38FGTKK847 pKa = 9.95SRR849 pKa = 11.84DD850 pKa = 3.48VVIQPAGVVPFTSHH864 pKa = 6.24HH865 pKa = 6.48VDD867 pKa = 3.4RR868 pKa = 11.84KK869 pKa = 10.31HH870 pKa = 5.82CTASYY875 pKa = 10.8DD876 pKa = 3.99RR877 pKa = 11.84PEE879 pKa = 4.3KK880 pKa = 10.56LAWNLFANCTSNDD893 pKa = 2.77FGLRR897 pKa = 11.84VDD899 pKa = 4.13AMLSVVRR906 pKa = 11.84NSPDD910 pKa = 3.23SLSKK914 pKa = 10.48FKK916 pKa = 10.89KK917 pKa = 9.71HH918 pKa = 6.33LGPFAQGTDD927 pKa = 3.36TCNKK931 pKa = 9.73DD932 pKa = 3.77LLWAII937 pKa = 4.69

Molecular weight: 102.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KE91|A0A1L3KE91_9VIRU Uncharacterized protein OS=Beihai sobemo-like virus 4 OX=1922701 PE=4 SV=1
MM1 pKa = 7.43ARR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84AIKK8 pKa = 9.41DD9 pKa = 3.05TTLTVGIKK17 pKa = 9.95QGTGAVTNAPFGSSGSHH34 pKa = 6.25RR35 pKa = 11.84PRR37 pKa = 11.84GGQRR41 pKa = 11.84SARR44 pKa = 11.84RR45 pKa = 11.84GSRR48 pKa = 11.84KK49 pKa = 9.6GRR51 pKa = 11.84GGGGRR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84GLNLSCWNAFAEE70 pKa = 4.28PHH72 pKa = 6.4LPLPRR77 pKa = 11.84AVAPYY82 pKa = 9.57TIIRR86 pKa = 11.84TTAIWNPSSDD96 pKa = 3.33HH97 pKa = 5.1QRR99 pKa = 11.84RR100 pKa = 11.84LALFGPIMDD109 pKa = 4.21VGSDD113 pKa = 2.83AGQWSNYY120 pKa = 8.67YY121 pKa = 10.37SISANVTLSSLRR133 pKa = 11.84NSANGAYY140 pKa = 9.94LSSFGSMSSSSWGAASVTPAAFSVQILNKK169 pKa = 9.91EE170 pKa = 4.08ALQTSTGMVYY180 pKa = 10.09IGRR183 pKa = 11.84CKK185 pKa = 10.8NKK187 pKa = 9.07VHH189 pKa = 6.57LAEE192 pKa = 5.34GDD194 pKa = 3.83NSTSWQDD201 pKa = 3.37LADD204 pKa = 3.89NLVSYY209 pKa = 10.18SNPRR213 pKa = 11.84MCSAGKK219 pKa = 9.04LALRR223 pKa = 11.84GVHH226 pKa = 6.33VDD228 pKa = 3.63AVPNNMSEE236 pKa = 4.17LAKK239 pKa = 9.89FTTLHH244 pKa = 5.74QSGDD248 pKa = 3.43APITIDD254 pKa = 3.42ATDD257 pKa = 3.82LTHH260 pKa = 7.5HH261 pKa = 6.66EE262 pKa = 4.38GFNPIFIYY270 pKa = 10.99NPDD273 pKa = 3.36AVALQVLVCCEE284 pKa = 3.28WRR286 pKa = 11.84VRR288 pKa = 11.84FDD290 pKa = 3.72PSNPAYY296 pKa = 10.08AACRR300 pKa = 11.84MHH302 pKa = 7.03KK303 pKa = 10.28PSTDD307 pKa = 3.48TSWAEE312 pKa = 3.83HH313 pKa = 5.81LQQAVALGNGVADD326 pKa = 3.27IVEE329 pKa = 4.45VVARR333 pKa = 11.84AGNALRR339 pKa = 11.84AVWW342 pKa = 3.49

Molecular weight: 36.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1279

342

937

639.5

69.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.946 ± 0.5

1.798 ± 0.021

7.115 ± 1.308

4.535 ± 1.053

3.987 ± 0.65

7.975 ± 0.382

2.893 ± 0.015

3.362 ± 0.351

4.848 ± 1.062

7.428 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.955 ± 0.044

3.753 ± 1.004

4.378 ± 0.284

3.206 ± 0.005

6.02 ± 0.758

9.226 ± 0.483

4.3 ± 0.742

7.975 ± 0.599

1.798 ± 0.259

2.502 ± 0.078
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski