Streptococcus phage IPP60

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5SF14|A0A1S5SF14_9CAUD Putative small subunit of the terminase OS=Streptococcus phage IPP60 OX=1916196 GN=IPP60_00037 PE=4 SV=1
MM1 pKa = 7.32IPKK4 pKa = 9.71FRR6 pKa = 11.84VWVKK10 pKa = 9.13IGKK13 pKa = 9.94RR14 pKa = 11.84MVFSDD19 pKa = 5.09DD20 pKa = 3.17ILAIDD25 pKa = 4.21YY26 pKa = 7.8EE27 pKa = 4.32NKK29 pKa = 10.46EE30 pKa = 3.92IVTQQVYY37 pKa = 9.68FEE39 pKa = 4.85SGLAVEE45 pKa = 4.77RR46 pKa = 11.84DD47 pKa = 3.38IYY49 pKa = 11.22CYY51 pKa = 10.95DD52 pKa = 3.57FDD54 pKa = 7.47DD55 pKa = 4.65IEE57 pKa = 4.99LMQSTGLKK65 pKa = 10.14DD66 pKa = 3.56KK67 pKa = 10.8NGKK70 pKa = 9.57EE71 pKa = 3.8IFEE74 pKa = 4.1GDD76 pKa = 2.94ILKK79 pKa = 10.86FNDD82 pKa = 3.03EE83 pKa = 4.11WNEE86 pKa = 3.91YY87 pKa = 8.78CHH89 pKa = 6.66EE90 pKa = 4.76GYY92 pKa = 10.91VDD94 pKa = 4.12GSVEE98 pKa = 3.79GVNYY102 pKa = 10.34VEE104 pKa = 4.4VVKK107 pKa = 11.26GEE109 pKa = 3.93ACFEE113 pKa = 4.26FGKK116 pKa = 8.67TRR118 pKa = 11.84YY119 pKa = 9.13PEE121 pKa = 4.12SSLFIYY127 pKa = 10.17MEE129 pKa = 4.56DD130 pKa = 3.22EE131 pKa = 4.15HH132 pKa = 8.96LSFAEE137 pKa = 4.08LVKK140 pKa = 11.17DD141 pKa = 3.37KK142 pKa = 11.42DD143 pKa = 3.67FGFEE147 pKa = 3.77IVGNVYY153 pKa = 10.76EE154 pKa = 4.11NLEE157 pKa = 4.09FLEE160 pKa = 4.71DD161 pKa = 3.35

Molecular weight: 18.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5SER8|A0A1S5SER8_9CAUD Uncharacterized protein OS=Streptococcus phage IPP60 OX=1916196 GN=IPP60_00051 PE=4 SV=1
MM1 pKa = 7.81RR2 pKa = 11.84PRR4 pKa = 11.84RR5 pKa = 11.84YY6 pKa = 9.18PYY8 pKa = 10.05SGKK11 pKa = 10.15KK12 pKa = 9.81EE13 pKa = 4.19STFVKK18 pKa = 10.34ADD20 pKa = 3.45PEE22 pKa = 4.2LVEE25 pKa = 4.92KK26 pKa = 10.39ILRR29 pKa = 11.84NISFLEE35 pKa = 3.81HH36 pKa = 6.02LQVVLATKK44 pKa = 10.33LL45 pKa = 3.41

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

13032

34

2402

206.9

23.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.052 ± 0.513

0.476 ± 0.06

6.231 ± 0.283

8.118 ± 0.47

4.197 ± 0.225

6.07 ± 0.478

1.443 ± 0.165

6.292 ± 0.237

8.679 ± 0.407

8.126 ± 0.24

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.463 ± 0.239

5.54 ± 0.291

2.785 ± 0.25

4.029 ± 0.227

4.543 ± 0.277

6.185 ± 0.418

6.139 ± 0.394

6.446 ± 0.283

1.289 ± 0.135

3.898 ± 0.296
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski