Psychrosphaera saromensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Pseudoalteromonadaceae; Psychrosphaera

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3139 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S7UZP6|A0A2S7UZP6_9GAMM dTDP-4-dehydrorhamnose reductase OS=Psychrosphaera saromensis OX=716813 GN=BTO11_14565 PE=3 SV=1
MM1 pKa = 7.93LSRR4 pKa = 11.84LRR6 pKa = 11.84NRR8 pKa = 11.84LIPINSALSTNLNSSSNVLSFSKK31 pKa = 10.64INFQMLDD38 pKa = 3.83LIKK41 pKa = 10.46RR42 pKa = 11.84SALIISTVFILAGCSSDD59 pKa = 4.12DD60 pKa = 3.59RR61 pKa = 11.84SSEE64 pKa = 4.02DD65 pKa = 3.18VVEE68 pKa = 4.22EE69 pKa = 4.39VEE71 pKa = 4.15AGNAVGYY78 pKa = 10.31VITVNGALIVDD89 pKa = 4.43WPQGFDD95 pKa = 3.83YY96 pKa = 10.67IDD98 pKa = 3.97AGAMVYY104 pKa = 10.71DD105 pKa = 4.2EE106 pKa = 5.99GGDD109 pKa = 3.51FYY111 pKa = 11.8VFTDD115 pKa = 3.56GDD117 pKa = 3.8SRR119 pKa = 11.84NIDD122 pKa = 3.26TSILGEE128 pKa = 4.08HH129 pKa = 5.99TVTFSLIDD137 pKa = 3.46SEE139 pKa = 4.97GNEE142 pKa = 4.22TVATRR147 pKa = 11.84TVNVFAPIPFITTWTTTEE165 pKa = 4.07SNQSIRR171 pKa = 11.84IPIYY175 pKa = 10.74SFNTYY180 pKa = 10.62YY181 pKa = 10.91DD182 pKa = 4.47FSIDD186 pKa = 3.47WGDD189 pKa = 3.44GSQIDD194 pKa = 4.07EE195 pKa = 4.39NQTDD199 pKa = 4.49SVTHH203 pKa = 6.74IYY205 pKa = 8.64STAGTYY211 pKa = 7.64TVSITGIFPHH221 pKa = 6.94MYY223 pKa = 9.66NDD225 pKa = 3.84EE226 pKa = 4.18NSSVDD231 pKa = 2.97KK232 pKa = 11.19LMSVEE237 pKa = 3.56QWGNIRR243 pKa = 11.84WSSMRR248 pKa = 11.84YY249 pKa = 6.27MFRR252 pKa = 11.84GAHH255 pKa = 5.54NLVINATDD263 pKa = 3.71APVLSDD269 pKa = 3.94VEE271 pKa = 4.35SMYY274 pKa = 11.69AMFYY278 pKa = 10.29GASSFNTDD286 pKa = 2.17ISHH289 pKa = 6.88WDD291 pKa = 3.44VSNVTDD297 pKa = 3.95MSVMFYY303 pKa = 10.84GAAAFNQPIDD313 pKa = 3.36GWDD316 pKa = 3.45VSNVTNMVWMFFNAAVFNQPLNSWDD341 pKa = 3.57VSNVTDD347 pKa = 3.77MSGMFYY353 pKa = 10.67VAAEE357 pKa = 4.22FNQPIDD363 pKa = 3.05SWDD366 pKa = 3.5ASNVTNMSFMFQLAEE381 pKa = 4.38SFNQPIDD388 pKa = 3.19SWDD391 pKa = 3.52VSNVTDD397 pKa = 3.89MSWMFGGTAAFNQPLDD413 pKa = 3.46SWDD416 pKa = 3.56VSNVTDD422 pKa = 3.71MSGMFAGAGVFNQPLDD438 pKa = 3.41SWDD441 pKa = 3.41VSRR444 pKa = 11.84VTNMSIMFHH453 pKa = 6.61RR454 pKa = 11.84LAAFNQPLDD463 pKa = 3.46SWDD466 pKa = 3.56VSNVTDD472 pKa = 3.96MEE474 pKa = 5.25SMFSGTTAFNQPLDD488 pKa = 3.54SWDD491 pKa = 3.58VSNVTTMINMLNSSGLSTDD510 pKa = 4.04NYY512 pKa = 11.16DD513 pKa = 3.61ALLNGWSIQILQPDD527 pKa = 4.91LIFDD531 pKa = 3.86VSATYY536 pKa = 10.57SSSSQAARR544 pKa = 11.84QSIIDD549 pKa = 3.82TYY551 pKa = 11.6NWTINDD557 pKa = 4.97DD558 pKa = 3.91GLQPP562 pKa = 4.09

Molecular weight: 62.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S7USA2|A0A2S7USA2_9GAMM Dipeptidase OS=Psychrosphaera saromensis OX=716813 GN=BTO11_03480 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.42RR12 pKa = 11.84KK13 pKa = 9.36RR14 pKa = 11.84SHH16 pKa = 6.15GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.39GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3139

0

3139

1031951

37

1982

328.8

36.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.835 ± 0.038

0.893 ± 0.014

5.885 ± 0.042

6.204 ± 0.042

4.399 ± 0.034

6.317 ± 0.04

1.99 ± 0.02

6.992 ± 0.036

6.231 ± 0.036

10.197 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.433 ± 0.021

5.112 ± 0.036

3.502 ± 0.027

4.397 ± 0.034

3.666 ± 0.028

7.08 ± 0.044

5.705 ± 0.031

6.866 ± 0.032

1.116 ± 0.018

3.18 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski