Vibrio phage ICP1_2011_A
Average proteome isoelectric point is 5.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 222 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A385IH78|A0A385IH78_9CAUD Uncharacterized protein OS=Vibrio phage ICP1_2011_A OX=1296592 GN=ICP12011A_219 PE=4 SV=1
MM1 pKa = 7.63 LYY3 pKa = 10.5 DD4 pKa = 3.49 VYY6 pKa = 10.97 RR7 pKa = 11.84 VDD9 pKa = 4.42 RR10 pKa = 11.84 HH11 pKa = 5.55 PHH13 pKa = 6.54 DD14 pKa = 4.06 IDD16 pKa = 5.12 DD17 pKa = 4.27 VVEE20 pKa = 4.19 TCIGVDD26 pKa = 3.67 LSDD29 pKa = 4.41 MEE31 pKa = 4.61 VKK33 pKa = 10.76 DD34 pKa = 6.27 LIATQNGYY42 pKa = 10.73 LEE44 pKa = 4.28 IFGVDD49 pKa = 3.55 SEE51 pKa = 4.61 GLVYY55 pKa = 10.91 DD56 pKa = 4.35 KK57 pKa = 11.4 VGEE60 pKa = 4.06
Molecular weight: 6.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.923
IPC_protein 3.859
Toseland 3.656
ProMoST 3.999
Dawson 3.872
Bjellqvist 4.101
Wikipedia 3.821
Rodwell 3.694
Grimsley 3.567
Solomon 3.846
Lehninger 3.808
Nozaki 3.999
DTASelect 4.228
Thurlkill 3.719
EMBOSS 3.834
Sillero 3.986
Patrickios 1.914
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.921
Protein with the highest isoelectric point:
>tr|A0A385IGW7|A0A385IGW7_9CAUD Uncharacterized protein OS=Vibrio phage ICP1_2011_A OX=1296592 GN=ICP12011A_119 PE=4 SV=1
MM1 pKa = 7.54 NNLKK5 pKa = 10.34 QKK7 pKa = 9.41 WNNLAKK13 pKa = 9.06 WQKK16 pKa = 9.94 ISAGIIGVVVAIYY29 pKa = 8.12 MASYY33 pKa = 10.63 LYY35 pKa = 10.82 GKK37 pKa = 7.86 TVFNPQQCYY46 pKa = 10.14 ALYY49 pKa = 10.72 GNRR52 pKa = 11.84 LFTAASLQALHH63 pKa = 6.37 VNSVGTTLPPIIKK76 pKa = 9.89 HH77 pKa = 5.16 GVVNGAKK84 pKa = 9.9 KK85 pKa = 10.26 RR86 pKa = 11.84 IHH88 pKa = 5.63 EE89 pKa = 4.56 CYY91 pKa = 9.66 MEE93 pKa = 4.54 GKK95 pKa = 10.12
Molecular weight: 10.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.164
IPC2_protein 9.487
IPC_protein 9.385
Toseland 9.911
ProMoST 9.721
Dawson 10.16
Bjellqvist 9.853
Wikipedia 10.335
Rodwell 10.628
Grimsley 10.233
Solomon 10.189
Lehninger 10.145
Nozaki 9.955
DTASelect 9.838
Thurlkill 9.999
EMBOSS 10.321
Sillero 10.072
Patrickios 10.16
IPC_peptide 10.175
IPC2_peptide 8.609
IPC2.peptide.svr19 8.309
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
222
0
222
36800
19
930
165.8
18.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.003 ± 0.235
1.791 ± 0.105
6.465 ± 0.141
7.704 ± 0.194
4.334 ± 0.107
6.321 ± 0.158
1.997 ± 0.087
6.345 ± 0.099
8.049 ± 0.202
8.473 ± 0.167
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.519 ± 0.084
5.375 ± 0.123
2.818 ± 0.116
3.418 ± 0.102
4.16 ± 0.138
6.446 ± 0.136
5.772 ± 0.188
6.739 ± 0.168
1.552 ± 0.076
4.72 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here