Mucilaginibacter gracilis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Mucilaginibacter

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5778 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A495J4Y1|A0A495J4Y1_9SPHI PKD domain-containing protein OS=Mucilaginibacter gracilis OX=423350 GN=BDD43_4253 PE=4 SV=1
MM1 pKa = 7.69EE2 pKa = 6.36DD3 pKa = 3.77EE4 pKa = 5.13NPDD7 pKa = 3.23DD8 pKa = 4.25VYY10 pKa = 11.74AVAEE14 pKa = 4.43DD15 pKa = 3.8PAPFEE20 pKa = 4.66PEE22 pKa = 4.6DD23 pKa = 3.68INIVNLRR30 pKa = 11.84DD31 pKa = 3.76LQWNVINLDD40 pKa = 3.41STRR43 pKa = 11.84FNIYY47 pKa = 9.56IYY49 pKa = 10.52LFKK52 pKa = 11.04EE53 pKa = 4.26PFCLIDD59 pKa = 3.16LHH61 pKa = 7.46

Molecular weight: 7.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A495IZQ7|A0A495IZQ7_9SPHI Cell division protein ZapA OS=Mucilaginibacter gracilis OX=423350 GN=BDD43_2386 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 10.49KK3 pKa = 10.45FILIIALAVTGSLYY17 pKa = 11.25ANAQTRR23 pKa = 11.84VVVTPARR30 pKa = 11.84KK31 pKa = 8.66VVVVRR36 pKa = 11.84RR37 pKa = 11.84PVRR40 pKa = 11.84PVVVAPARR48 pKa = 11.84AVVVRR53 pKa = 11.84PHH55 pKa = 4.9VTVVRR60 pKa = 11.84PVVRR64 pKa = 11.84KK65 pKa = 9.16RR66 pKa = 11.84VVIVHH71 pKa = 6.44HH72 pKa = 6.59

Molecular weight: 7.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5778

0

5778

1913951

29

2792

331.2

37.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.704 ± 0.028

0.82 ± 0.011

5.339 ± 0.02

5.123 ± 0.034

4.775 ± 0.026

6.668 ± 0.034

1.906 ± 0.015

7.258 ± 0.026

6.998 ± 0.034

9.58 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.252 ± 0.014

5.801 ± 0.027

3.839 ± 0.018

3.971 ± 0.023

3.851 ± 0.021

6.222 ± 0.026

6.136 ± 0.041

6.342 ± 0.024

1.147 ± 0.013

4.269 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski