Streptomyces phage ShakeNBake

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 104 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G9W2Q0|A0A7G9W2Q0_9CAUD Uncharacterized protein OS=Streptomyces phage ShakeNBake OX=2768142 GN=96 PE=4 SV=1
MM1 pKa = 8.16DD2 pKa = 5.08EE3 pKa = 4.22NTNTEE8 pKa = 3.75HH9 pKa = 7.68DD10 pKa = 3.97KK11 pKa = 11.83YY12 pKa = 10.66EE13 pKa = 4.07LAVQISVLSYY23 pKa = 10.89DD24 pKa = 4.35LNVANPTVPTLLKK37 pKa = 10.85EE38 pKa = 4.09NTEE41 pKa = 4.14DD42 pKa = 3.54QLIAKK47 pKa = 9.4RR48 pKa = 11.84DD49 pKa = 3.66SLLAMIDD56 pKa = 3.7EE57 pKa = 4.44QVSLDD62 pKa = 3.5GFYY65 pKa = 10.69EE66 pKa = 4.75VPAHH70 pKa = 7.1MDD72 pKa = 3.48ATWLCTLLQTEE83 pKa = 4.66LGAYY87 pKa = 9.99VDD89 pKa = 4.07VDD91 pKa = 3.76PEE93 pKa = 4.22AEE95 pKa = 3.93QGKK98 pKa = 10.31CIVLVDD104 pKa = 4.0PSS106 pKa = 3.38

Molecular weight: 11.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G9W2F7|A0A7G9W2F7_9CAUD Uncharacterized protein OS=Streptomyces phage ShakeNBake OX=2768142 GN=3 PE=4 SV=1
MM1 pKa = 7.35HH2 pKa = 7.57RR3 pKa = 11.84NGYY6 pKa = 9.94CSLADD11 pKa = 3.33SSSKK15 pKa = 10.11HH16 pKa = 5.63KK17 pKa = 10.43RR18 pKa = 11.84YY19 pKa = 9.71EE20 pKa = 4.14CAWCGRR26 pKa = 11.84VRR28 pKa = 11.84RR29 pKa = 11.84SDD31 pKa = 3.12RR32 pKa = 11.84MRR34 pKa = 11.84RR35 pKa = 11.84WSGRR39 pKa = 11.84MEE41 pKa = 4.42CKK43 pKa = 10.08DD44 pKa = 3.3VHH46 pKa = 6.59SCARR50 pKa = 11.84AQRR53 pKa = 11.84QRR55 pKa = 11.84GMEE58 pKa = 3.93KK59 pKa = 10.36AAA61 pKa = 4.16

Molecular weight: 7.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

104

0

104

20523

46

1397

197.3

21.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.296 ± 0.456

1.223 ± 0.138

7.236 ± 0.192

7.09 ± 0.406

2.811 ± 0.133

7.869 ± 0.256

2.008 ± 0.191

4.376 ± 0.193

4.99 ± 0.346

7.889 ± 0.238

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.807 ± 0.187

3.289 ± 0.163

4.722 ± 0.201

3.815 ± 0.175

6.344 ± 0.364

5.696 ± 0.375

5.979 ± 0.461

6.807 ± 0.272

1.944 ± 0.153

2.811 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski