Amino acid dipepetide frequency for Clostridium sp. CAG:306

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.766AlaAla: 5.766 ± 0.143	0.929AlaCys: 0.929 ± 0.048	4.034AlaAsp: 4.034 ± 0.09	4.134AlaGlu: 4.134 ± 0.099	3.0AlaPhe: 3.0 ± 0.074	4.803AlaGly: 4.803 ± 0.127	1.177AlaHis: 1.177 ± 0.043	5.56AlaIle: 5.56 ± 0.116	6.756AlaLys: 6.756 ± 0.128	6.96AlaLeu: 6.96 ± 0.124	2.111AlaMet: 2.111 ± 0.062	3.487AlaAsn: 3.487 ± 0.09	2.152AlaPro: 2.152 ± 0.074	3.749AlaGln: 3.749 ± 0.094	2.371AlaArg: 2.371 ± 0.064	3.992AlaSer: 3.992 ± 0.087	3.034AlaThr: 3.034 ± 0.081	5.079AlaVal: 5.079 ± 0.092	0.377AlaTrp: 0.377 ± 0.027	2.541AlaTyr: 2.541 ± 0.077	0.003AlaXaa: 0.003 ± 0.003
Cys	1.086CysAla: 1.086 ± 0.045	0.249CysCys: 0.249 ± 0.021	0.794CysAsp: 0.794 ± 0.033	0.849CysGlu: 0.849 ± 0.044	0.637CysPhe: 0.637 ± 0.032	0.958CysGly: 0.958 ± 0.044	0.216CysHis: 0.216 ± 0.018	0.947CysIle: 0.947 ± 0.042	1.008CysLys: 1.008 ± 0.037	0.925CysLeu: 0.925 ± 0.039	0.299CysMet: 0.299 ± 0.022	0.618CysAsn: 0.618 ± 0.034	0.6CysPro: 0.6 ± 0.038	0.292CysGln: 0.292 ± 0.022	0.364CysArg: 0.364 ± 0.023	0.81CysSer: 0.81 ± 0.036	0.642CysThr: 0.642 ± 0.033	0.834CysVal: 0.834 ± 0.037	0.105CysTrp: 0.105 ± 0.012	0.484CysTyr: 0.484 ± 0.025	0.0CysXaa: 0.0 ± 0.0
Asp	3.76AspAla: 3.76 ± 0.09	0.77AspCys: 0.77 ± 0.037	3.02AspAsp: 3.02 ± 0.085	4.415AspGlu: 4.415 ± 0.089	3.299AspPhe: 3.299 ± 0.079	3.339AspGly: 3.339 ± 0.098	0.54AspHis: 0.54 ± 0.027	5.669AspIle: 5.669 ± 0.107	5.581AspLys: 5.581 ± 0.101	4.735AspLeu: 4.735 ± 0.086	1.566AspMet: 1.566 ± 0.057	3.318AspAsn: 3.318 ± 0.081	1.613AspPro: 1.613 ± 0.056	0.873AspGln: 0.873 ± 0.04	1.855AspArg: 1.855 ± 0.06	3.312AspSer: 3.312 ± 0.087	3.04AspThr: 3.04 ± 0.074	3.752AspVal: 3.752 ± 0.074	0.487AspTrp: 0.487 ± 0.025	2.748AspTyr: 2.748 ± 0.08	0.002AspXaa: 0.002 ± 0.002
Glu	4.591GluAla: 4.591 ± 0.095	0.669GluCys: 0.669 ± 0.033	3.441GluAsp: 3.441 ± 0.076	5.134GluGlu: 5.134 ± 0.119	3.109GluPhe: 3.109 ± 0.072	2.921GluGly: 2.921 ± 0.074	1.121GluHis: 1.121 ± 0.044	6.159GluIle: 6.159 ± 0.11	7.173GluLys: 7.173 ± 0.116	6.496GluLeu: 6.496 ± 0.122	1.747GluMet: 1.747 ± 0.056	4.912GluAsn: 4.912 ± 0.11	1.795GluPro: 1.795 ± 0.06	2.794GluGln: 2.794 ± 0.074	2.347GluArg: 2.347 ± 0.051	3.218GluSer: 3.218 ± 0.058	3.551GluThr: 3.551 ± 0.077	3.529GluVal: 3.529 ± 0.082	0.393GluTrp: 0.393 ± 0.026	2.732GluTyr: 2.732 ± 0.063	0.002GluXaa: 0.002 ± 0.002
Phe	3.232PheAla: 3.232 ± 0.078	0.741PheCys: 0.741 ± 0.04	3.294PheAsp: 3.294 ± 0.08	3.275PheGlu: 3.275 ± 0.088	2.033PhePhe: 2.033 ± 0.069	2.611PheGly: 2.611 ± 0.069	0.616PheHis: 0.616 ± 0.034	3.564PheIle: 3.564 ± 0.092	3.808PheLys: 3.808 ± 0.087	3.962PheLeu: 3.962 ± 0.092	1.071PheMet: 1.071 ± 0.043	2.807PheAsn: 2.807 ± 0.078	1.21PhePro: 1.21 ± 0.045	1.158PheGln: 1.158 ± 0.042	1.271PheArg: 1.271 ± 0.05	3.189PheSer: 3.189 ± 0.086	2.14PheThr: 2.14 ± 0.062	3.032PheVal: 3.032 ± 0.077	0.326PheTrp: 0.326 ± 0.027	1.745PheTyr: 1.745 ± 0.059	0.002PheXaa: 0.002 ± 0.002
Gly	4.509GlyAla: 4.509 ± 0.132	0.917GlyCys: 0.917 ± 0.046	2.89GlyAsp: 2.89 ± 0.078	3.294GlyGlu: 3.294 ± 0.074	2.853GlyPhe: 2.853 ± 0.071	4.022GlyGly: 4.022 ± 0.188	1.063GlyHis: 1.063 ± 0.036	4.878GlyIle: 4.878 ± 0.104	4.872GlyLys: 4.872 ± 0.1	5.115GlyLeu: 5.115 ± 0.103	1.632GlyMet: 1.632 ± 0.054	2.882GlyAsn: 2.882 ± 0.098	1.051GlyPro: 1.051 ± 0.053	1.737GlyGln: 1.737 ± 0.056	2.095GlyArg: 2.095 ± 0.058	3.519GlySer: 3.519 ± 0.105	3.216GlyThr: 3.216 ± 0.096	3.943GlyVal: 3.943 ± 0.081	0.533GlyTrp: 0.533 ± 0.03	2.644GlyTyr: 2.644 ± 0.068	0.0GlyXaa: 0.0 ± 0.0
His	0.963HisAla: 0.963 ± 0.041	0.249HisCys: 0.249 ± 0.02	0.774HisAsp: 0.774 ± 0.034	0.883HisGlu: 0.883 ± 0.035	0.746HisPhe: 0.746 ± 0.035	0.985HisGly: 0.985 ± 0.042	0.367HisHis: 0.367 ± 0.027	1.329HisIle: 1.329 ± 0.049	1.215HisLys: 1.215 ± 0.045	1.29HisLeu: 1.29 ± 0.046	0.329HisMet: 0.329 ± 0.023	0.928HisAsn: 0.928 ± 0.042	0.774HisPro: 0.774 ± 0.032	0.453HisGln: 0.453 ± 0.026	0.522HisArg: 0.522 ± 0.031	1.054HisSer: 1.054 ± 0.038	0.8HisThr: 0.8 ± 0.035	0.784HisVal: 0.784 ± 0.035	0.152HisTrp: 0.152 ± 0.016	0.632HisTyr: 0.632 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	6.173IleAla: 6.173 ± 0.119	1.1IleCys: 1.1 ± 0.042	5.108IleAsp: 5.108 ± 0.095	5.986IleGlu: 5.986 ± 0.107	3.379IlePhe: 3.379 ± 0.085	4.498IleGly: 4.498 ± 0.107	1.131IleHis: 1.131 ± 0.044	6.079IleIle: 6.079 ± 0.126	7.657IleLys: 7.657 ± 0.127	6.952IleLeu: 6.952 ± 0.137	1.753IleMet: 1.753 ± 0.057	5.375IleAsn: 5.375 ± 0.117	3.01IlePro: 3.01 ± 0.069	2.521IleGln: 2.521 ± 0.063	2.563IleArg: 2.563 ± 0.06	5.429IleSer: 5.429 ± 0.089	4.669IleThr: 4.669 ± 0.084	4.923IleVal: 4.923 ± 0.089	0.436IleTrp: 0.436 ± 0.027	2.716IleTyr: 2.716 ± 0.066	0.002IleXaa: 0.002 ± 0.002
Lys	6.205LysAla: 6.205 ± 0.122	0.851LysCys: 0.851 ± 0.04	5.849LysAsp: 5.849 ± 0.102	7.534LysGlu: 7.534 ± 0.125	3.467LysPhe: 3.467 ± 0.081	4.401LysGly: 4.401 ± 0.094	1.381LysHis: 1.381 ± 0.044	7.606LysIle: 7.606 ± 0.133	8.224LysLys: 8.224 ± 0.147	7.463LysLeu: 7.463 ± 0.133	2.338LysMet: 2.338 ± 0.058	6.932LysAsn: 6.932 ± 0.12	2.861LysPro: 2.861 ± 0.079	3.465LysGln: 3.465 ± 0.085	3.036LysArg: 3.036 ± 0.073	4.819LysSer: 4.819 ± 0.099	5.53LysThr: 5.53 ± 0.1	4.658LysVal: 4.658 ± 0.087	0.525LysTrp: 0.525 ± 0.034	3.826LysTyr: 3.826 ± 0.077	0.0LysXaa: 0.0 ± 0.0
Leu	6.339LeuAla: 6.339 ± 0.111	1.306LeuCys: 1.306 ± 0.049	4.891LeuAsp: 4.891 ± 0.093	5.843LeuGlu: 5.843 ± 0.119	3.867LeuPhe: 3.867 ± 0.102	5.131LeuGly: 5.131 ± 0.112	1.38LeuHis: 1.38 ± 0.056	6.64LeuIle: 6.64 ± 0.115	8.92LeuLys: 8.92 ± 0.138	7.358LeuLeu: 7.358 ± 0.148	2.207LeuMet: 2.207 ± 0.06	5.78LeuAsn: 5.78 ± 0.119	3.264LeuPro: 3.264 ± 0.08	2.842LeuGln: 2.842 ± 0.066	2.98LeuArg: 2.98 ± 0.08	6.018LeuSer: 6.018 ± 0.107	4.998LeuThr: 4.998 ± 0.104	4.821LeuVal: 4.821 ± 0.097	0.596LeuTrp: 0.596 ± 0.032	3.253LeuTyr: 3.253 ± 0.08	0.005LeuXaa: 0.005 ± 0.003
Met	1.966MetAla: 1.966 ± 0.064	0.227MetCys: 0.227 ± 0.02	1.378MetAsp: 1.378 ± 0.045	1.535MetGlu: 1.535 ± 0.048	1.065MetPhe: 1.065 ± 0.042	1.536MetGly: 1.536 ± 0.053	0.404MetHis: 0.404 ± 0.026	1.841MetIle: 1.841 ± 0.055	1.982MetLys: 1.982 ± 0.054	2.381MetLeu: 2.381 ± 0.067	0.667MetMet: 0.667 ± 0.032	1.533MetAsn: 1.533 ± 0.048	1.242MetPro: 1.242 ± 0.046	1.121MetGln: 1.121 ± 0.046	0.795MetArg: 0.795 ± 0.033	1.768MetSer: 1.768 ± 0.063	1.499MetThr: 1.499 ± 0.047	1.415MetVal: 1.415 ± 0.059	0.158MetTrp: 0.158 ± 0.013	0.787MetTyr: 0.787 ± 0.04	0.0MetXaa: 0.0 ± 0.0
Asn	4.417AsnAla: 4.417 ± 0.09	0.76AsnCys: 0.76 ± 0.039	3.069AsnAsp: 3.069 ± 0.083	3.63AsnGlu: 3.63 ± 0.079	2.858AsnPhe: 2.858 ± 0.07	3.594AsnGly: 3.594 ± 0.112	0.811AsnHis: 0.811 ± 0.037	5.712AsnIle: 5.712 ± 0.12	5.581AsnLys: 5.581 ± 0.104	5.581AsnLeu: 5.581 ± 0.093	1.56AsnMet: 1.56 ± 0.053	4.233AsnAsn: 4.233 ± 0.1	2.761AsnPro: 2.761 ± 0.065	1.846AsnGln: 1.846 ± 0.065	1.881AsnArg: 1.881 ± 0.054	3.633AsnSer: 3.633 ± 0.087	3.471AsnThr: 3.471 ± 0.078	3.444AsnVal: 3.444 ± 0.085	0.479AsnTrp: 0.479 ± 0.025	2.547AsnTyr: 2.547 ± 0.073	0.002AsnXaa: 0.002 ± 0.002
Pro	2.405ProAla: 2.405 ± 0.079	0.438ProCys: 0.438 ± 0.036	2.449ProAsp: 2.449 ± 0.069	2.804ProGlu: 2.804 ± 0.074	1.562ProPhe: 1.562 ± 0.047	1.496ProGly: 1.496 ± 0.053	0.533ProHis: 0.533 ± 0.031	2.186ProIle: 2.186 ± 0.053	2.464ProLys: 2.464 ± 0.066	2.747ProLeu: 2.747 ± 0.074	0.738ProMet: 0.738 ± 0.035	1.594ProAsn: 1.594 ± 0.056	0.949ProPro: 0.949 ± 0.041	1.56ProGln: 1.56 ± 0.054	0.921ProArg: 0.921 ± 0.041	2.039ProSer: 2.039 ± 0.062	1.555ProThr: 1.555 ± 0.056	3.136ProVal: 3.136 ± 0.083	0.233ProTrp: 0.233 ± 0.019	1.4ProTyr: 1.4 ± 0.052	0.002ProXaa: 0.002 ± 0.002
Gln	2.6GlnAla: 2.6 ± 0.075	0.331GlnCys: 0.331 ± 0.025	1.749GlnAsp: 1.749 ± 0.056	2.619GlnGlu: 2.619 ± 0.073	1.145GlnPhe: 1.145 ± 0.047	1.848GlnGly: 1.848 ± 0.054	0.473GlnHis: 0.473 ± 0.026	2.919GlnIle: 2.919 ± 0.077	3.957GlnLys: 3.957 ± 0.087	2.739GlnLeu: 2.739 ± 0.075	1.032GlnMet: 1.032 ± 0.041	2.628GlnAsn: 2.628 ± 0.064	0.995GlnPro: 0.995 ± 0.048	1.59GlnGln: 1.59 ± 0.062	1.314GlnArg: 1.314 ± 0.043	1.982GlnSer: 1.982 ± 0.057	2.031GlnThr: 2.031 ± 0.063	1.76GlnVal: 1.76 ± 0.056	0.259GlnTrp: 0.259 ± 0.021	1.368GlnTyr: 1.368 ± 0.05	0.002GlnXaa: 0.002 ± 0.002
Arg	2.346ArgAla: 2.346 ± 0.064	0.423ArgCys: 0.423 ± 0.029	1.646ArgAsp: 1.646 ± 0.052	2.216ArgGlu: 2.216 ± 0.06	1.547ArgPhe: 1.547 ± 0.047	1.701ArgGly: 1.701 ± 0.054	0.589ArgHis: 0.589 ± 0.032	2.93ArgIle: 2.93 ± 0.067	2.576ArgLys: 2.576 ± 0.068	3.155ArgLeu: 3.155 ± 0.082	0.901ArgMet: 0.901 ± 0.045	1.919ArgAsn: 1.919 ± 0.054	1.103ArgPro: 1.103 ± 0.051	1.241ArgGln: 1.241 ± 0.046	1.252ArgArg: 1.252 ± 0.053	1.704ArgSer: 1.704 ± 0.055	1.673ArgThr: 1.673 ± 0.049	2.103ArgVal: 2.103 ± 0.054	0.287ArgTrp: 0.287 ± 0.023	1.444ArgTyr: 1.444 ± 0.049	0.002ArgXaa: 0.002 ± 0.002
Ser	4.334SerAla: 4.334 ± 0.09	0.679SerCys: 0.679 ± 0.035	3.61SerAsp: 3.61 ± 0.094	3.483SerGlu: 3.483 ± 0.078	2.999SerPhe: 2.999 ± 0.076	3.96SerGly: 3.96 ± 0.108	0.985SerHis: 0.985 ± 0.042	4.596SerIle: 4.596 ± 0.081	5.027SerLys: 5.027 ± 0.099	5.649SerLeu: 5.649 ± 0.098	1.531SerMet: 1.531 ± 0.053	3.36SerAsn: 3.36 ± 0.092	1.816SerPro: 1.816 ± 0.061	2.387SerGln: 2.387 ± 0.069	2.002SerArg: 2.002 ± 0.063	4.121SerSer: 4.121 ± 0.094	3.197SerThr: 3.197 ± 0.077	4.099SerVal: 4.099 ± 0.091	0.388SerTrp: 0.388 ± 0.027	2.41SerTyr: 2.41 ± 0.068	0.002SerXaa: 0.002 ± 0.002
Thr	4.136ThrAla: 4.136 ± 0.092	0.517ThrCys: 0.517 ± 0.029	3.294ThrAsp: 3.294 ± 0.074	3.128ThrGlu: 3.128 ± 0.069	2.228ThrPhe: 2.228 ± 0.061	3.903ThrGly: 3.903 ± 0.098	0.806ThrHis: 0.806 ± 0.036	4.275ThrIle: 4.275 ± 0.089	4.334ThrLys: 4.334 ± 0.089	4.944ThrLeu: 4.944 ± 0.101	1.167ThrMet: 1.167 ± 0.041	3.072ThrAsn: 3.072 ± 0.075	2.397ThrPro: 2.397 ± 0.058	2.168ThrGln: 2.168 ± 0.063	1.709ThrArg: 1.709 ± 0.051	3.098ThrSer: 3.098 ± 0.076	3.031ThrThr: 3.031 ± 0.074	3.58ThrVal: 3.58 ± 0.09	0.271ThrTrp: 0.271 ± 0.023	1.86ThrTyr: 1.86 ± 0.054	0.002ThrXaa: 0.002 ± 0.002
Val	4.093ValAla: 4.093 ± 0.102	0.912ValCys: 0.912 ± 0.034	3.553ValAsp: 3.553 ± 0.075	4.094ValGlu: 4.094 ± 0.085	3.024ValPhe: 3.024 ± 0.072	3.347ValGly: 3.347 ± 0.086	0.845ValHis: 0.845 ± 0.039	5.036ValIle: 5.036 ± 0.103	5.563ValLys: 5.563 ± 0.106	5.771ValLeu: 5.771 ± 0.106	1.626ValMet: 1.626 ± 0.051	3.481ValAsn: 3.481 ± 0.075	2.065ValPro: 2.065 ± 0.057	1.967ValGln: 1.967 ± 0.061	1.851ValArg: 1.851 ± 0.064	4.148ValSer: 4.148 ± 0.087	3.313ValThr: 3.313 ± 0.068	4.172ValVal: 4.172 ± 0.092	0.401ValTrp: 0.401 ± 0.025	2.312ValTyr: 2.312 ± 0.055	0.0ValXaa: 0.0 ± 0.0
Trp	0.46TrpAla: 0.46 ± 0.029	0.097TrpCys: 0.097 ± 0.013	0.374TrpAsp: 0.374 ± 0.027	0.425TrpGlu: 0.425 ± 0.027	0.318TrpPhe: 0.318 ± 0.025	0.506TrpGly: 0.506 ± 0.037	0.161TrpHis: 0.161 ± 0.017	0.509TrpIle: 0.509 ± 0.026	0.476TrpLys: 0.476 ± 0.028	0.752TrpLeu: 0.752 ± 0.032	0.212TrpMet: 0.212 ± 0.018	0.407TrpAsn: 0.407 ± 0.026	0.16TrpPro: 0.16 ± 0.017	0.321TrpGln: 0.321 ± 0.022	0.2TrpArg: 0.2 ± 0.017	0.315TrpSer: 0.315 ± 0.021	0.3TrpThr: 0.3 ± 0.021	0.426TrpVal: 0.426 ± 0.025	0.075TrpTrp: 0.075 ± 0.014	0.287TrpTyr: 0.287 ± 0.023	0.002TrpXaa: 0.002 ± 0.002
Tyr	2.636TyrAla: 2.636 ± 0.069	0.551TyrCys: 0.551 ± 0.033	2.475TyrAsp: 2.475 ± 0.083	2.55TyrGlu: 2.55 ± 0.08	1.95TyrPhe: 1.95 ± 0.061	2.106TyrGly: 2.106 ± 0.071	0.631TyrHis: 0.631 ± 0.034	3.028TyrIle: 3.028 ± 0.073	3.791TyrLys: 3.791 ± 0.089	3.463TyrLeu: 3.463 ± 0.077	0.81TyrMet: 0.81 ± 0.037	2.6TyrAsn: 2.6 ± 0.078	1.391TyrPro: 1.391 ± 0.046	1.249TyrGln: 1.249 ± 0.053	1.429TyrArg: 1.429 ± 0.047	2.465TyrSer: 2.465 ± 0.073	2.228TyrThr: 2.228 ± 0.061	2.084TyrVal: 2.084 ± 0.072	0.302TyrTrp: 0.302 ± 0.026	1.718TyrTyr: 1.718 ± 0.064	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.002XaaAsp: 0.002 ± 0.002	0.002XaaGlu: 0.002 ± 0.002	0.003XaaPhe: 0.003 ± 0.002	0.002XaaGly: 0.002 ± 0.002	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.008XaaLys: 0.008 ± 0.003	0.0XaaLeu: 0.0 ± 0.0	0.002XaaMet: 0.002 ± 0.002	0.003XaaAsn: 0.003 ± 0.002	0.002XaaPro: 0.002 ± 0.002	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.002XaaSer: 0.002 ± 0.002	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.021XaaXaa: 0.021 ± 0.008

Statistics based on 2130 proteins (626252 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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